Protein of unknown function (DUF674)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 294 |
| AccI | GTMKAC | 1 cut(s) | 463 |
| AciI | CCGC | 1 cut(s) | 5 |
| AclWI | GGATC | 2 cut(s) | 142, 260 |
| AcoI | YGGCCR | 1 cut(s) | 201 |
| AcuI | CTGAAG | 1 cut(s) | 384 |
| AfaI | GTAC | 1 cut(s) | 95 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 78, 294 |
| AjnI | CCWGG | 1 cut(s) | 287 |
| AluBI | AGCT | 4 cut(s) | 281, 329, 404, 451 |
| AluI | AGCT | 4 cut(s) | 281, 329, 404, 451 |
| Alw21I | GWGCWC | 1 cut(s) | 283 |
| Alw26I | GTCTC | 1 cut(s) | 46 |
| AlwI | GGATC | 2 cut(s) | 142, 260 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 294 |
| AoxI | GGCC | 2 cut(s) | 201, 285 |
| ApeKI | GCWGC | 1 cut(s) | 371 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 329, 361 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 397 |
| AspS9I | GGNCC | 2 cut(s) | 245, 345 |
| AvaII | GGWCC | 2 cut(s) | 245, 345 |
| BanII | GRGCYC | 1 cut(s) | 283 |
| Bbv12I | GWGCWC | 1 cut(s) | 283 |
| BbvI | GCAGC | 1 cut(s) | 383 |
| BceAI | ACGGC | 1 cut(s) | 196 |
| BciT130I | CCWGG | 1 cut(s) | 289 |
| BclI | TGATCA | 1 cut(s) | 301 |
| BcoDI | GTCTC | 1 cut(s) | 46 |
| BisI | GCNGC | 1 cut(s) | 372 |
| BlsI | GCNGC | 1 cut(s) | 373 |
| Bme1390I | CCNGG | 1 cut(s) | 289 |
| Bme18I | GGWCC | 2 cut(s) | 245, 345 |
| BmgT120I | GGNCC | 2 cut(s) | 245, 345 |
| BmrFI | CCNGG | 1 cut(s) | 289 |
| BoxI | GACNNNNGTC | 1 cut(s) | 243 |
| BpmI | CTGGAG | 1 cut(s) | 155 |
| Bpu10I | CCTNAGC | 1 cut(s) | 282 |
| BsaI | GGTCTC | 1 cut(s) | 46 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 77, 107 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 78, 294 |
| Bse1I | ACTGG | 1 cut(s) | 214 |
| BseBI | CCWGG | 1 cut(s) | 289 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 78, 294 |
| BseMII | CTCAG | 1 cut(s) | 296 |
| BseNI | ACTGG | 1 cut(s) | 214 |
| BseRI | GAGGAG | 2 cut(s) | 23, 116 |
| BseXI | GCAGC | 1 cut(s) | 383 |
| BseYI | CCCAGC | 1 cut(s) | 329 |
| BshFI | GGCC | 2 cut(s) | 203, 287 |
| BsiHKAI | GWGCWC | 1 cut(s) | 283 |
| BslFI | GGGAC | 3 cut(s) | 146, 346, 438 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 78, 294 |
| BsmAI | GTCTC | 1 cut(s) | 46 |
| BsmFI | GGGAC | 3 cut(s) | 146, 346, 438 |
| BsnI | GGCC | 2 cut(s) | 203, 287 |
| Bso31I | GGTCTC | 1 cut(s) | 46 |
| Bsp1286I | GDGCHC | 1 cut(s) | 283 |
| Bsp143I | GATC | 3 cut(s) | 147, 265, 301 |
| BspACI | CCGC | 1 cut(s) | 5 |
| BspANI | GGCC | 2 cut(s) | 203, 287 |
| BspCNI | CTCAG | 1 cut(s) | 295 |
| BspPI | GGATC | 2 cut(s) | 142, 260 |
| BspTNI | GGTCTC | 1 cut(s) | 46 |
| BsrI | ACTGG | 1 cut(s) | 214 |
| BssMI | GATC | 3 cut(s) | 147, 265, 301 |
| Bst2UI | CCWGG | 1 cut(s) | 289 |
| Bst4CI | ACNGT | 1 cut(s) | 489 |
| BstDEI | CTNAG | 1 cut(s) | 282 |
| BstKTI | GATC | 3 cut(s) | 150, 268, 304 |
| BstMAI | GTCTC | 1 cut(s) | 46 |
| BstMBI | GATC | 3 cut(s) | 147, 265, 301 |
| BstNI | CCWGG | 1 cut(s) | 289 |
| BstNSI | RCATGY | 1 cut(s) | 43 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 243 |
| BstSCI | CCNGG | 1 cut(s) | 287 |
| BstV1I | GCAGC | 1 cut(s) | 383 |
| BsuRI | GGCC | 2 cut(s) | 203, 287 |
| BtsI | GCAGTG | 1 cut(s) | 125 |
| BtsIMutI | CAGTG | 1 cut(s) | 125 |
| CaiI | CAGNNNCTG | 1 cut(s) | 294 |
| Cfr13I | GGNCC | 2 cut(s) | 245, 345 |
| Csp6I | GTAC | 1 cut(s) | 94 |
| CviAII | CATG | 2 cut(s) | 40, 49 |
| CviJI | RGCY | 9 cut(s) | 27, 203, 230, 281, 287, 329, 374, 404, 451 |
| CviKI_1 | RGCY | 9 cut(s) | 27, 203, 230, 281, 287, 329, 374, 404, 451 |
| CviQI | GTAC | 1 cut(s) | 94 |
| DdeI | CTNAG | 1 cut(s) | 282 |
| DpnI | GATC | 3 cut(s) | 149, 267, 303 |
| DpnII | GATC | 3 cut(s) | 147, 265, 301 |
| EaeI | YGGCCR | 1 cut(s) | 201 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Ecl136II | GAGCTC | 1 cut(s) | 281 |
| Eco24I | GRGCYC | 1 cut(s) | 283 |
| Eco31I | GGTCTC | 1 cut(s) | 46 |
| Eco47I | GGWCC | 2 cut(s) | 245, 345 |
| Eco53kI | GAGCTC | 1 cut(s) | 281 |
| Eco57I | CTGAAG | 1 cut(s) | 384 |
| EcoICRI | GAGCTC | 1 cut(s) | 281 |
| EcoO109I | RGGNCCY | 1 cut(s) | 245 |
| EcoRII | CCWGG | 1 cut(s) | 287 |
| EcoT38I | GRGCYC | 1 cut(s) | 283 |
| FaeI | CATG | 2 cut(s) | 43, 52 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 420, 452 |
| FaqI | GGGAC | 3 cut(s) | 146, 346, 438 |
| FatI | CATG | 2 cut(s) | 39, 48 |
| FbaI | TGATCA | 1 cut(s) | 301 |
| FblI | GTMKAC | 1 cut(s) | 463 |
| Fnu4HI | GCNGC | 1 cut(s) | 372 |
| FriOI | GRGCYC | 1 cut(s) | 283 |
| Fsp4HI | GCNGC | 1 cut(s) | 372 |
| GluI | GCNGC | 1 cut(s) | 372 |
| GsaI | CCCAGC | 1 cut(s) | 333 |
| GsuI | CTGGAG | 1 cut(s) | 155 |
| HaeIII | GGCC | 2 cut(s) | 203, 287 |
| Hin1II | CATG | 2 cut(s) | 43, 52 |
| HinfI | GANTC | 2 cut(s) | 142, 197 |
| Hpy166II | GTNNAC | 2 cut(s) | 348, 464 |
| Hpy188I | TCNGA | 3 cut(s) | 69, 147, 265 |
| Hpy188III | TCNNGA | 1 cut(s) | 134 |
| Hpy8I | GTNNAC | 2 cut(s) | 348, 464 |
| HpyAV | CCTTC | 3 cut(s) | 229, 373, 408 |
| HpyCH4III | ACNGT | 1 cut(s) | 489 |
| HpyF3I | CTNAG | 1 cut(s) | 282 |
| Hsp92II | CATG | 2 cut(s) | 43, 52 |
| Ksp22I | TGATCA | 1 cut(s) | 301 |
| Kzo9I | GATC | 3 cut(s) | 147, 265, 301 |
| Lsp1109I | GCAGC | 1 cut(s) | 383 |
| MaeIII | GTNAC | 1 cut(s) | 476 |
| MalI | GATC | 3 cut(s) | 149, 267, 303 |
| MboI | GATC | 3 cut(s) | 147, 265, 301 |
| MboII | GAAGA | 1 cut(s) | 371 |
| MhlI | GDGCHC | 1 cut(s) | 283 |
| MlyI | GAGTC | 1 cut(s) | 206 |
| MmeI | TCCRAC | 1 cut(s) | 388 |
| MnlI | CCTC | 7 cut(s) | 4, 94, 97, 133, 220, 271, 404 |
| MroXI | GAANNNNTTC | 1 cut(s) | 397 |
| MspA1I | CMGCKG | 1 cut(s) | 404 |
| MspR9I | CCNGG | 1 cut(s) | 289 |
| MvaI | CCWGG | 1 cut(s) | 289 |
| NdeII | GATC | 3 cut(s) | 147, 265, 301 |
| NlaIII | CATG | 2 cut(s) | 43, 52 |
| NmuCI | GTSAC | 1 cut(s) | 476 |
| NspI | RCATGY | 1 cut(s) | 43 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 186 |
| PdmI | GAANNNNTTC | 1 cut(s) | 397 |
| PfeI | GAWTC | 1 cut(s) | 142 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 294 |
| PkrI | GCNGC | 1 cut(s) | 373 |
| PleI | GAGTC | 1 cut(s) | 205 |
| PpsI | GAGTC | 1 cut(s) | 205 |
| PpuMI | RGGWCCY | 1 cut(s) | 245 |
| PshAI | GACNNNNGTC | 1 cut(s) | 243 |
| Psp124BI | GAGCTC | 1 cut(s) | 283 |
| Psp5II | RGGWCCY | 1 cut(s) | 245 |
| Psp6I | CCWGG | 1 cut(s) | 287 |
| PspFI | CCCAGC | 1 cut(s) | 329 |
| PspGI | CCWGG | 1 cut(s) | 287 |
| PspPI | GGNCC | 2 cut(s) | 245, 345 |
| PspPPI | RGGWCCY | 1 cut(s) | 245 |
| PstNI | CAGNNNCTG | 1 cut(s) | 294 |
| PvuII | CAGCTG | 1 cut(s) | 404 |
| RsaI | GTAC | 1 cut(s) | 95 |
| RsaNI | GTAC | 1 cut(s) | 94 |
| SacI | GAGCTC | 1 cut(s) | 283 |
| SatI | GCNGC | 1 cut(s) | 372 |
| Sau3AI | GATC | 3 cut(s) | 147, 265, 301 |
| Sau96I | GGNCC | 2 cut(s) | 245, 345 |
| SchI | GAGTC | 1 cut(s) | 206 |
| ScrFI | CCNGG | 1 cut(s) | 289 |
| SduI | GDGCHC | 1 cut(s) | 283 |
| SinI | GGWCC | 2 cut(s) | 245, 345 |
| SsiI | CCGC | 1 cut(s) | 5 |
| SstI | GAGCTC | 1 cut(s) | 283 |
| StyD4I | CCNGG | 1 cut(s) | 287 |
| TaaI | ACNGT | 1 cut(s) | 489 |
| TaqI | TCGA | 1 cut(s) | 35 |
| TaqII | GACCGA | 1 cut(s) | 86 |
| TatI | WGTACW | 1 cut(s) | 93 |
| TfiI | GAWTC | 1 cut(s) | 142 |
| TscAI | CASTG | 1 cut(s) | 132 |
| TseFI | GTSAC | 1 cut(s) | 476 |
| TseI | GCWGC | 1 cut(s) | 371 |
| Tsp45I | GTSAC | 1 cut(s) | 476 |
| TspDTI | ATGAA | 2 cut(s) | 155, 339 |
| TspRI | CASTG | 1 cut(s) | 132 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 294 |
| VpaK11BI | GGWCC | 2 cut(s) | 245, 345 |
| XceI | RCATGY | 1 cut(s) | 43 |
| XmiI | GTMKAC | 1 cut(s) | 463 |
| XmnI | GAANNNNTTC | 1 cut(s) | 397 |