pycom03g06330

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000148
Physical Location & Seq
Reverse (-)
4950312 .. 4951470
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06330.2

Sequence Viewer

Length: 1065 bp
ATGTTATCTTCAGTTTCGACAATGCCCATGGGAAGAATTGTCAGGCTTGCCTGTAAATCTTCAGTACCGGTGGAAATAGGTTGTATGAGAAATTTGTATCAGAGTATTGCGCAATTTAATGTGCATAAGTTCCGGTCAGTGGCATGTGGACTTATGTTGTTACTTCCCTGCAATGCTGCTGATTGTCAGTGCAAGAACCTCAAGTTGAAAATTGACAAGGATGTGCCGACACGGTACCTTTTGTGCTCTGATGGTTGCTTCTTAAGTTATTATAGAGATATCCGTTGTCCTTCCTGTGGATCTCTTTTTAGTCGGGGGACAGATTTTCCAGTGGATAATACTCAAGTTGGAGGCGTCTTTGTGAAAGGGCAACCCCGGCTGATAATTACCGATGATTTACAGGTCATATCCCCAGTAAGTGCATTAAGCATTTGTTTGTTTTCGAAGCTAGGGGTCACAGATTCTAAATTTACGGAGGAGTTGACGTTCAATATGGGAGTTCAGGAGGCTTTGAATTTGCTTATGCATTCGTTTGTATCAAAGACACCGTTGACTGAAATTCTTCTGAAAAACGAACAAATACCAAGTTTGGGAGATGTACATTCCAGTCAAGGAATATCGATTCATTCTCAAATGCATGGCGACACAATCGATGAGGAAGAGAAAAACATTTCTCTTAAGCTTGTAGTTAGCAAGTCTAAAAAGATTGTTTGTTATGCAGAAGCACAAGAGGATTTTTTTAATCTACTCTGCAGTTTCTTGACTCTCCCACTTGGGTTCATATTAGAAAAGATGCAGAATGTTTCATGGAAGGGCTGTCTCGGTCAGTTGTACAAGAGTGTCGAAGATTTTGATGAGCAATACTTGAAGTCAAATTTCCACAAGGAATTGTTGGTTAATCCAAAGCTTGCACCTGGTTTTCGATACGAGAAAACTCTGTTAGGAATCGAGGAGACGTCCTTTTATTATGTTAAGTCTAAGTTAACTACTGATAAGTCCTGTATTCCTGATAATACTTCAGCGGAGTCAGTTAAACTTAATGTTGTGGATCCCAAATCTTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

355

Amino Acids

39.49

Weight (kDa)

6.96

Isoelectric Point (pI)

42.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 959
Acc16I TGCGCA 1 cut(s) 111
Acc65I GGTACC 1 cut(s) 234
AccB1I GGYRCC 1 cut(s) 234
AciI CCGC 1 cut(s) 1022
AclWI GGATC 3 cut(s) 307, 1043, 1056
AcsI RAATTY 5 cut(s) 91, 467, 514, 558, 874
AcuI CTGAAG 2 cut(s) 45, 1002
AcyI GRCGYC 2 cut(s) 354, 956
AfaI GTAC 4 cut(s) 66, 236, 600, 833
AfiI CCNNNNNNNGG 3 cut(s) 139, 296, 590
AflII CTTAAG 2 cut(s) 262, 677
AgeI ACCGGT 1 cut(s) 67
AgsI TTSAA 4 cut(s) 208, 490, 514, 868
AjnI CCWGG 1 cut(s) 913
AloI GAACNNNNNNTCC 2 cut(s) 470, 502
AluBI AGCT 3 cut(s) 448, 682, 907
AluI AGCT 3 cut(s) 448, 682, 907
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 2 cut(s) 824, 947
AlwI GGATC 3 cut(s) 307, 1043, 1056
ApeKI GCWGC 1 cut(s) 176
ApoI RAATTY 5 cut(s) 91, 467, 514, 558, 874
AsiGI ACCGGT 1 cut(s) 67
Asp700I GAANNNNTTC 1 cut(s) 561
Asp718I GGTACC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 112
AsuC2I CCSGG 1 cut(s) 376
AsuII TTCGAA 1 cut(s) 443
BamHI GGATCC 1 cut(s) 1048
BanI GGYRCC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 248
BbvI GCAGC 1 cut(s) 163
BccI CCATC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 915
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 824, 947
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 1 cut(s) 751
BfrI CTTAAG 2 cut(s) 262, 677
BisI GCNGC 1 cut(s) 177
BlsI GCNGC 1 cut(s) 178
Bme1390I CCNGG 2 cut(s) 376, 915
BmiI GGNNCC 2 cut(s) 236, 1050
BmrFI CCNGG 2 cut(s) 376, 915
BmrI ACTGGG 1 cut(s) 407
BmsI GCATC 1 cut(s) 783
BmuI ACTGGG 1 cut(s) 407
Bpu14I TTCGAA 1 cut(s) 443
BpuEI CTTGAG 2 cut(s) 185, 327
BpuMI CCSGG 1 cut(s) 376
Bsa29I ATCGAT 2 cut(s) 620, 651
BsaHI GRCGYC 2 cut(s) 354, 956
BsaJI CCNNGG 2 cut(s) 27, 374
BsaWI WCCGGW 2 cut(s) 67, 132
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 296, 590
Bse118I RCCGGY 1 cut(s) 67
Bse1I ACTGG 3 cut(s) 329, 413, 606
Bse3DI GCAATG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 915
BseCI ATCGAT 2 cut(s) 620, 651
BseDI CCNNGG 2 cut(s) 27, 374
BseGI GGATG 1 cut(s) 226
BseLI CCNNNNNNNGG 3 cut(s) 139, 296, 590
BseMI GCAATG 1 cut(s) 178
BseNI ACTGG 3 cut(s) 329, 413, 606
BseRI GAGGAG 2 cut(s) 491, 965
BseXI GCAGC 1 cut(s) 163
BshNI GGYRCC 1 cut(s) 234
BshTI ACCGGT 1 cut(s) 67
BshVI ATCGAT 2 cut(s) 620, 651
BsiHKAI GWGCWC 1 cut(s) 248
BsiSI CCGG 3 cut(s) 68, 133, 376
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 3 cut(s) 139, 296, 590
BsmAI GTCTC 2 cut(s) 824, 947
BsmBI CGTCTC 1 cut(s) 947
BsmFI GGGAC 1 cut(s) 331
BsmI GAATGC 1 cut(s) 526
Bsp119I TTCGAA 1 cut(s) 443
Bsp1286I GDGCHC 1 cut(s) 248
Bsp1407I TGTACA 2 cut(s) 598, 831
Bsp143I GATC 2 cut(s) 299, 1048
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 1 cut(s) 1022
BspDI ATCGAT 2 cut(s) 620, 651
BspHI TCATGA 1 cut(s) 1061
BspLI GGNNCC 2 cut(s) 236, 1050
BspMAI CTGCAG 1 cut(s) 755
BspPI GGATC 3 cut(s) 307, 1043, 1056
BspT104I TTCGAA 1 cut(s) 443
BspT107I GGYRCC 1 cut(s) 234
BspTI CTTAAG 2 cut(s) 262, 677
BsrDI GCAATG 1 cut(s) 178
BsrFI RCCGGY 1 cut(s) 67
BsrGI TGTACA 2 cut(s) 598, 831
BsrI ACTGG 3 cut(s) 329, 413, 606
BssAI RCCGGY 1 cut(s) 67
BssECI CCNNGG 2 cut(s) 27, 374
BssMI GATC 2 cut(s) 299, 1048
BssNI GRCGYC 2 cut(s) 354, 956
BssT1I CCWWGG 1 cut(s) 27
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 2 cut(s) 234, 549
Bst6I CTCTTC 1 cut(s) 654
BstACI GRCGYC 2 cut(s) 354, 956
BstAFI CTTAAG 2 cut(s) 262, 677
BstAUI TGTACA 2 cut(s) 598, 831
BstBI TTCGAA 1 cut(s) 443
BstC8I GCNNGC 2 cut(s) 48, 909
BstDEI CTNAG 1 cut(s) 978
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 226
BstHHI GCGC 1 cut(s) 112
BstKTI GATC 2 cut(s) 302, 1051
BstMAI GTCTC 2 cut(s) 824, 947
BstMBI GATC 2 cut(s) 299, 1048
BstMWI GCNNNNNNNGC 1 cut(s) 376
BstNI CCWGG 1 cut(s) 915
BstNSI RCATGY 1 cut(s) 147
BstSCI CCNGG 2 cut(s) 374, 913
BstSFI CTRYAG 1 cut(s) 751
BstV1I GCAGC 1 cut(s) 163
BstX2I RGATCY 2 cut(s) 299, 1048
BstYI RGATCY 2 cut(s) 299, 1048
Bsu15I ATCGAT 2 cut(s) 620, 651
BsuTUI ATCGAT 2 cut(s) 620, 651
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 226
BtsIMutI CAGTG 3 cut(s) 144, 194, 336
Cac8I GCNNGC 2 cut(s) 48, 909
CciI TCATGA 1 cut(s) 1061
CfoI GCGC 1 cut(s) 112
Cfr10I RCCGGY 1 cut(s) 67
ClaI ATCGAT 2 cut(s) 620, 651
CseI GACGC 1 cut(s) 343
CsiI ACCWGGT 1 cut(s) 913
Csp6I GTAC 4 cut(s) 65, 235, 599, 832
CspAI ACCGGT 1 cut(s) 67
CviAII CATG 5 cut(s) 28, 144, 638, 807, 1062
CviJI RGCY 7 cut(s) 46, 379, 448, 509, 682, 816, 907
CviKI_1 RGCY 7 cut(s) 46, 379, 448, 509, 682, 816, 907
CviQI GTAC 4 cut(s) 65, 235, 599, 832
DdeI CTNAG 1 cut(s) 978
DpnI GATC 2 cut(s) 301, 1050
DpnII GATC 2 cut(s) 299, 1048
Eam1104I CTCTTC 1 cut(s) 654
EarI CTCTTC 1 cut(s) 654
Eco130I CCWWGG 1 cut(s) 27
Eco32I GATATC 1 cut(s) 280
Eco57I CTGAAG 2 cut(s) 45, 1002
EcoRII CCWGG 1 cut(s) 913
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 1 cut(s) 27
EcoT22I ATGCAT 2 cut(s) 528, 639
ErhI CCWWGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 947
FaeI CATG 5 cut(s) 31, 147, 641, 810, 1065
FaqI GGGAC 1 cut(s) 331
FatI CATG 5 cut(s) 27, 143, 637, 806, 1061
Fnu4HI GCNGC 1 cut(s) 177
FokI GGATG 1 cut(s) 233
Fsp4HI GCNGC 1 cut(s) 177
FspBI CTAG 1 cut(s) 449
FspI TGCGCA 1 cut(s) 111
GlaI GCGC 1 cut(s) 111
GluI GCNGC 1 cut(s) 177
HapII CCGG 3 cut(s) 68, 133, 376
HgaI GACGC 1 cut(s) 343
HhaI GCGC 1 cut(s) 112
Hin1I GRCGYC 2 cut(s) 354, 956
Hin1II CATG 5 cut(s) 31, 147, 641, 810, 1065
Hin6I GCGC 1 cut(s) 110
HinP1I GCGC 1 cut(s) 110
HincII GTYRAC 3 cut(s) 483, 552, 984
HindII GTYRAC 3 cut(s) 483, 552, 984
HindIII AAGCTT 2 cut(s) 680, 905
HinfI GANTC 5 cut(s) 461, 622, 763, 945, 1025
HpaI GTTAAC 1 cut(s) 984
HpaII CCGG 3 cut(s) 68, 133, 376
Hpy166II GTNNAC 4 cut(s) 149, 483, 552, 984
Hpy188I TCNGA 3 cut(s) 102, 250, 567
Hpy188III TCNNGA 4 cut(s) 503, 760, 1007, 1062
Hpy8I GTNNAC 4 cut(s) 149, 483, 552, 984
HpyAV CCTTC 2 cut(s) 300, 805
HpyCH4III ACNGT 2 cut(s) 234, 549
HpyCH4IV ACGT 2 cut(s) 485, 956
HpyF10VI GCNNNNNNNGC 1 cut(s) 376
HpyF3I CTNAG 1 cut(s) 978
HpySE526I ACGT 2 cut(s) 485, 956
Hsp92I GRCGYC 2 cut(s) 354, 956
Hsp92II CATG 5 cut(s) 31, 147, 641, 810, 1065
HspAI GCGC 1 cut(s) 110
KpnI GGTACC 1 cut(s) 238
KspAI GTTAAC 1 cut(s) 984
Kzo9I GATC 2 cut(s) 299, 1048
Lsp1109I GCAGC 1 cut(s) 163
LweI GCATC 1 cut(s) 783
MabI ACCWGGT 1 cut(s) 913
MaeI CTAG 1 cut(s) 449
MaeII ACGT 2 cut(s) 485, 956
MaeIII GTNAC 2 cut(s) 159, 454
MalI GATC 2 cut(s) 301, 1050
MboI GATC 2 cut(s) 299, 1048
MboII GAAGA 6 cut(s) 45, 51, 554, 671, 857, 1050
MflI RGATCY 2 cut(s) 299, 1048
MhlI GDGCHC 1 cut(s) 248
MlyI GAGTC 2 cut(s) 757, 1034
MmeI TCCRAC 1 cut(s) 328
MnlI CCTC 7 cut(s) 209, 344, 469, 499, 649, 724, 943
Mph1103I ATGCAT 2 cut(s) 528, 639
MroXI GAANNNNTTC 1 cut(s) 561
MspA1I CMGCKG 1 cut(s) 1022
MspCI CTTAAG 2 cut(s) 262, 677
MspI CCGG 3 cut(s) 68, 133, 376
MspR9I CCNGG 2 cut(s) 376, 915
Mva1269I GAATGC 1 cut(s) 526
MvaI CCWGG 1 cut(s) 915
MwoI GCNNNNNNNGC 1 cut(s) 376
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 1 cut(s) 27
NdeII GATC 2 cut(s) 299, 1048
NlaIII CATG 5 cut(s) 31, 147, 641, 810, 1065
NlaIV GGNNCC 2 cut(s) 236, 1050
NmuCI GTSAC 1 cut(s) 454
NsbI TGCGCA 1 cut(s) 111
NsiI ATGCAT 2 cut(s) 528, 639
NspI RCATGY 1 cut(s) 147
NspV TTCGAA 1 cut(s) 443
PagI TCATGA 1 cut(s) 1061
PctI GAATGC 1 cut(s) 526
PdmI GAANNNNTTC 1 cut(s) 561
PfeI GAWTC 3 cut(s) 461, 622, 945
PinAI ACCGGT 1 cut(s) 67
PkrI GCNGC 1 cut(s) 178
PleI GAGTC 2 cut(s) 757, 1033
PpsI GAGTC 2 cut(s) 757, 1033
Psp6I CCWGG 1 cut(s) 913
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 2 cut(s) 236, 1050
PstI CTGCAG 1 cut(s) 755
PsuI RGATCY 2 cut(s) 299, 1048
RsaI GTAC 4 cut(s) 66, 236, 600, 833
RsaNI GTAC 4 cut(s) 65, 235, 599, 832
SatI GCNGC 1 cut(s) 177
Sau3AI GATC 2 cut(s) 299, 1048
SchI GAGTC 2 cut(s) 757, 1034
ScrFI CCNGG 2 cut(s) 376, 915
SduI GDGCHC 1 cut(s) 248
SexAI ACCWGGT 1 cut(s) 913
SfaNI GCATC 1 cut(s) 783
SfcI CTRYAG 1 cut(s) 751
SfuI TTCGAA 1 cut(s) 443
SmlI CTYRAG 4 cut(s) 200, 262, 342, 677
SmoI CTYRAG 4 cut(s) 200, 262, 342, 677
SsiI CCGC 1 cut(s) 1022
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 2 cut(s) 374, 913
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 2 cut(s) 234, 549
TaiI ACGT 2 cut(s) 488, 959
TaqI TCGA 7 cut(s) 17, 443, 620, 651, 843, 922, 948
TaqII GACCGA 1 cut(s) 812
TatI WGTACW 2 cut(s) 598, 831
TfiI GAWTC 3 cut(s) 461, 622, 945
TscAI CASTG 3 cut(s) 144, 194, 336
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 4 cut(s) 614, 769, 795, 1050
TspGWI ACGGA 2 cut(s) 272, 488
TspRI CASTG 3 cut(s) 144, 194, 336
Vha464I CTTAAG 2 cut(s) 262, 677
XapI RAATTY 5 cut(s) 91, 467, 514, 558, 874
XceI RCATGY 1 cut(s) 147
XmnI GAANNNNTTC 1 cut(s) 561
XspI CTAG 1 cut(s) 449
ZraI GACGTC 1 cut(s) 957
Zsp2I ATGCAT 2 cut(s) 528, 639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.