Rorug05G0192400

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000148
Physical Location & Seq
Reverse (-)
18865771 .. 18870756
4986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0192400.1

Sequence Viewer

Length: 3303 bp
ATGGTTACTTCTTCCCTGAAAATGCTATTACACTTGGGACTCCTCGTGTTATGTTTCAGTTTTGGCTCTACAAACTCATTAGATGAAGAAAGTCTTTTTCTTTTGGAGTTCAAGAGATCCCTATCTGATCCAAGGAACAATCTTGGAAGCTGGAATTCATCACATTTGAGTCCTTGCAATTGGACTGGTGTGAGGTGTCTCAAGTCCAAGGTAACTTCTATAAATCTCACTGGCCTTAATTTGTCTGGTGTTTTGTCTCCAGTCATTTGCAATCTTCCTTACTTGGTTGAATTCAATGTGTCCAAGAACTTCTTCTCTGGACCATTTCCCAATGATCTTGCTAACTGTCATAATCTAGCGTTTCTAGACCTTTGCACAAATAGGTTTCATGGGGAATTGGTTATTCCATTCACCAAAATGACCAACCTTAGGAAGTTGTATCTCTGTGAGAATTATGTGTTTGGTGAAATGCCTGAGGAGATAGGGAACTTAACTCTACTTGAAGAGCTTGTGATTTATAGTAATAATCTCACTGGCTCCATTCCTGGTTCAATTAGTAAGTTGAAAAAGTTAAAAGTCCTCAGGGCGGGTCGAAATTCTCTTTCTGGCCCAATACCAACTGTTATCAGTCAGTGTCAGAGCTTAGAAGTTTTGGGGTTATCACAGAATCATTTAGAAGGTTCAATTCCCAGGGAGCTTGAAGAACTTCAAAATCTGACTGATTTGATCCTTTGGCAAAATCATTTAACTGGTTCAGTTCCTGCTGAGATTGGTAATCTAAGTAGGCTAGAGTTGCTTGCCTTGCATGAAAACTCTTTCGGAGGAATGATCCCTAAGGAACTTGGGAAGTTATCACAGCTGAAGAGATTGTATATCTACACCAACCAGTTGAATGGAACTATCCCAAGTGAACTAGGGAATTGTACCAATGCGGTTCATATAGATTTTTCTGAAAACCAGTTAAGTGGAGTCATACCACGGGAGTTGGGTTACATTCCTAATCTTCTGTTGCTTCACCTCTTCGAAAATCATCTGGAAGGAAACATTCCGAGGGAGCTTGGCCAGTTGAAGCAGCTGCAGAAGTTAGACTTGTCCATAAACAATTTGACTGGCCCAATCCCACTAGAATTTCAGAACCTTACTTGCATGGATGAGTTGCAACTGTTTGATAATCATCTCGAGGGTAAAATTCCTCCTCTCCTTGGAGCTAACAGTAACCTCAGCATTCTTGACATGTCTGCAAATAAGCTTGTGGGTAGCATACCTGCGCATCTTTGCGAGTATGGGAAATTAGCATTTCTAAGCCTTGGGTCAAATAGGTTGTCTGGAAATATTCCTTATGGTATTAAAACATGCAAGTCTCTAGTCCAGCTAATGCTAGGAGACAACCATCTTACAGGAAGTCTCCCTATGGAGTTATATACTCTTTATGCTCTTGAAGTCTTTCAAAACCGATTCTCAGGACCTATACCTCCAGAGGTAGGCAGGTTTAAAAGTTTAGAAAGGCTACTGTTGTCAGATAACTATTTTATTGGGCATATTCCTCTTGAGATTGGCAACCTATCGCAGCTTGTGACCTTCAATGTTTCCTCTAACAGGCTCACGGGGAACATTCCTCACGAGCTGGGAAATTGTACAAAGCTACAGAGGCTTGATCTCAGTAGGAATTACTTCACTGGAGTTCTCCCGGAAGAACTTGGAAAGCTAGTGAATCTGGAACTTTTGAAGCTTTCTGATAACAAGTTGATGGGAGGGATACCGAGTACTCTAGGGGACCTTGCCCGATTGACTGAGTTGGAGATGGGGGGAAATCACTTTTCTGGTAGCATCCCTTTTCAGCTGGGCCAACTCGGTGCTCTACAGATTGCCCTCAATATTAGCCATAATAATCTTTCGGGTGAAATTCCTGAAAAGTTGGGGGACTTGCAGATGTTGGAATCCCTCTACTTGAATGACAATCAGCTTGTTGGTGAAATTCCTGCCTCAATTGGTGAGTTGCTTAGCCTTCTGGTGTGCAACCTCTCTAACAATAACCTAGTGGGAACTGTGCCAAACACACAAGTATTTCAAAGGATGGATTCTTCAAATTTTGCTGGAAACAATGGATTGTGCAGATCTGGATCATACCATTGTCATCAACCTCCAGTTCAATCTCATACTCCAAAACGGAGCTGGATAAAGGAGGGTTCATCTAAAGAGAAATTAGTAAGCATCATTGCTGCTGTAATTGGAGTAATTTCCTTGATTCTCATAGTTGGTTTCTGTTGGGCAATGAAGCGCTCCAGACCTACTTTTGTTCCACTTGAAGATCCAATCAAGCCAGACGTGTTGGACAACTATTACTTTCCAAAGGAAGGTTTCAAGTACCAGGATCTTGTTGTAGGCACCAACAATTTTTCAGACAATGTAGTTCTTGGAAGGGGAGCCTGTGGCACTGTGTACAAAGCCGTCATGGCTGATGGGGAGGTGATTGCAGTCAAAAAGCTCAAGGCACAAGGGGAAGGAGTCGGTGTCGATAGCAGCTTCCGCGCTGAGATATCAACCCTCGGGAAGATCAGGCATTGCAATATTGTGAAGCTCTATGGCTTCTGCTGTCACCAAGACTCGAATCTGCTCTTGTATGAGTACATGGAAAATGGCAGTTTAGGAGAACATCTCCATGGAAACGATCAAAGATGTTATCTAGACTGGAATACACGATGTAAGATTGCTCTTGGAGCAGCAGAGGGTTTGTGTTATCTCCATTATTACTGCAAGCCCCAAATCGTCCACCGTGACATAAAGTCAAATAATATCTTACTGGATGAAGTTCTTGAAGCACATGTGGGAGATTTCGGCTTGGCAAAATTGATTGAATTGCCATACTCAAAATCCATGTCTGCTGTTGCTGGCTCATATGGCTACATTGCACCAGAGTATGCTTACACAATGAAAGTGACTGAGAAGTGTGACATCTATAGTTTCGGAGTGGTTCTGCTGGAACTAGTAACCGGAAAGACACCCGTCCAACCACTGGAGGTGGGTGGAGACCTAGTCACTCTGGTAAGAAGAGCCATAAATAATTCAGTGCCAACATCCGAATTGTTCGACAAGAGGCTAGATCTGAGTATGAGGAGTACAACTGAAGAGATGACTCTATTTCTCAAGATTGCGTTGTTCTGTACCAGTGTGTCCCCAGTTAAAAGGCCAACAATGAGGGAGGTCATTGCGATGATGATTGATGCTAGGCGGTCCGTGACTGTGAGCAACTGCTCATCACCAACATCTGAGAGTCCTTTGGATGAAGATCCTTCAAGAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

1100

Amino Acids

121.39

Weight (kDa)

5.97

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 66 8.5e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 113 - 242 4e-13 Leucine-rich repeat region
LRR_8 PF13855 141 - 201 8e-06 Leucine rich repeat
LRR_8 PF13855 499 - 557 8.4e-06 Leucine rich repeat
LRR_8 PF13855 547 - 606 4.3e-08 Leucine rich repeat
Pkinase PF00069 801 - 1070 2.8e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 804 - 1069 8.4e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1269
Acc36I ACCTGC 2 cut(s) 1273, 1476
AccB1I GGYRCC 1 cut(s) 2384
AccB7I CCANNNNNTGG 1 cut(s) 3013
AccII CGCG 1 cut(s) 2529
AciI CCGC 4 cut(s) 587, 932, 2527, 3229
AclWI GGATC 8 cut(s) 111, 122, 721, 823, 2128, 2303, 2379, 3281
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 8 cut(s) 154, 290, 595, 1127, 1188, 1902, 1974, 2086
AcuI CTGAAG 2 cut(s) 881, 3144
AfaI GTAC 8 cut(s) 925, 1636, 1765, 2366, 2441, 2627, 3118, 3163
AfeI AGCGCT 1 cut(s) 2279
AfiI CCNNNNNNNGG 8 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013, 3296
AflIII ACRYGT 3 cut(s) 1233, 2325, 2821
AhdI GACNNNNNGTC 1 cut(s) 2782
AhlI ACTAGT 1 cut(s) 2983
AjiI CACGTC 1 cut(s) 2326
AjnI CCWGG 3 cut(s) 544, 689, 2367
AjuI GAANNNNNNNTTGG 2 cut(s) 296, 328
Alw21I GWGCWC 1 cut(s) 1858
Alw26I GTCTC 6 cut(s) 203, 261, 1365, 1377, 1409, 3021
AlwI GGATC 8 cut(s) 111, 122, 721, 823, 2128, 2303, 2379, 3281
AlwNI CAGNNNCTG 1 cut(s) 761
Ama87I CYCGRG 2 cut(s) 1178, 2546
Aor51HI AGCGCT 1 cut(s) 2279
AoxI GGCC 6 cut(s) 232, 607, 1060, 1111, 1843, 3185
ApeKI GCWGC 6 cut(s) 1072, 1075, 1567, 2219, 2520, 2720
ApoI RAATTY 8 cut(s) 154, 290, 595, 1127, 1188, 1902, 1974, 2086
ArsI GACNNNNNNTTYG 4 cut(s) 1307, 1339, 2861, 2893
Asp700I GAANNNNTTC 4 cut(s) 311, 705, 1443, 1670
AspLEI GCGC 3 cut(s) 1270, 2280, 2531
AspS9I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
AsuC2I CCSGG 1 cut(s) 1688
AsuHPI GGTGA 9 cut(s) 403, 476, 1007, 1910, 1982, 2003, 2479, 2588, 3249
AsuII TTCGAA 1 cut(s) 1023
AvaI CYCGRG 2 cut(s) 1178, 2546
AvaII GGWCC 4 cut(s) 320, 1463, 1774, 3231
AxyI CCTNAGG 4 cut(s) 428, 474, 581, 834
BalI TGGCCA 1 cut(s) 1062
BanI GGYRCC 1 cut(s) 2384
BauI CACGAG 2 cut(s) 44, 1619
Bbv12I GWGCWC 1 cut(s) 1858
BbvCI CCTCAGC 1 cut(s) 1220
BbvI GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
BccI CCATC 5 cut(s) 1398, 1741, 1795, 2068, 2453
BceAI ACGGC 1 cut(s) 2432
BciT130I CCWGG 3 cut(s) 546, 691, 2369
BciVI GTATCC 1 cut(s) 1749
BcnI CCSGG 1 cut(s) 1688
BcoDI GTCTC 6 cut(s) 203, 261, 1365, 1377, 1409, 3021
BcuI ACTAGT 1 cut(s) 2983
BfmI CTRYAG 4 cut(s) 1076, 1643, 1859, 2956
BfoI RGCGCY 1 cut(s) 2281
BfuAI ACCTGC 2 cut(s) 1273, 1476
BfuI GTATCC 1 cut(s) 1749
BglI GCCNNNNNGGC 1 cut(s) 2453
BglII AGATCT 2 cut(s) 2114, 3100
BisI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
BlpI GCTNAGC 1 cut(s) 2000
BlsI GCNGC 6 cut(s) 1074, 1077, 1569, 2221, 2522, 2722
BmcAI AGTACT 1 cut(s) 1765
Bme1390I CCNGG 4 cut(s) 546, 691, 1688, 2369
Bme18I GGWCC 4 cut(s) 320, 1463, 1774, 3231
BmeRI GACNNNNNGTC 1 cut(s) 2782
BmeT110I CYCGRG 2 cut(s) 1178, 2546
BmgBI CACGTC 1 cut(s) 2326
BmgT120I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
BmiI GGNNCC 4 cut(s) 538, 1775, 2386, 2425
BmrFI CCNGG 4 cut(s) 546, 691, 1688, 2369
BmrI ACTGGG 1 cut(s) 3170
BmsI GCATC 4 cut(s) 1279, 1836, 2220, 3211
BmuI ACTGGG 1 cut(s) 3170
BoxI GACNNNNGTC 1 cut(s) 3002
BplI GAGNNNNNCTC 2 cut(s) 2640, 2672
BpmI CTGGAG 6 cut(s) 243, 1458, 1698, 2127, 2266, 3035
Bpu10I CCTNAGC 1 cut(s) 1220
Bpu1102I GCTNAGC 1 cut(s) 2000
Bpu14I TTCGAA 1 cut(s) 1023
BpuEI CTTGAG 4 cut(s) 185, 1568, 2471, 3128
BpuMI CCSGG 1 cut(s) 1688
BsaBI GATNNNNATC 4 cut(s) 121, 1173, 2119, 3285
BsaI GGTCTC 1 cut(s) 3021
BsaWI WCCGGW 1 cut(s) 2990
BsaXI ACNNNNNCTCC 4 cut(s) 2496, 2526, 3018, 3048
Bsc4I CCNNNNNNNGG 8 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013, 3296
Bse21I CCTNAGG 4 cut(s) 428, 474, 581, 834
Bse3DI GCAATG 5 cut(s) 2214, 2277, 2560, 2904, 3204
Bse8I GATNNNNATC 4 cut(s) 121, 1173, 2119, 3285
BseBI CCWGG 3 cut(s) 546, 691, 2369
BseGI GGATG 6 cut(s) 1156, 1827, 2079, 2809, 3074, 3286
BseJI GATNNNNATC 4 cut(s) 121, 1173, 2119, 3285
BseLI CCNNNNNNNGG 8 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013, 3296
BseMI GCAATG 5 cut(s) 2214, 2277, 2560, 2904, 3204
BseRI GAGGAG 4 cut(s) 32, 491, 1185, 3127
BseXI GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
BseYI CCCAGC 2 cut(s) 1624, 1840
BsgI GTGCAG 1 cut(s) 2131
Bsh1236I CGCG 1 cut(s) 2529
BshFI GGCC 6 cut(s) 234, 609, 1062, 1113, 1845, 3187
BshNI GGYRCC 1 cut(s) 2384
BsiHKAI GWGCWC 1 cut(s) 1858
BsiHKCI CYCGRG 2 cut(s) 1178, 2546
BsiSI CCGG 2 cut(s) 1688, 2991
BslFI GGGAC 4 cut(s) 51, 1787, 1934, 3157
BslI CCNNNNNNNGG 8 cut(s) 429, 586, 1202, 1481, 1596, 2354, 3013, 3296
BsmAI GTCTC 6 cut(s) 203, 261, 1365, 1377, 1409, 3021
BsmFI GGGAC 4 cut(s) 51, 1787, 1934, 3157
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 6 cut(s) 234, 609, 1062, 1113, 1845, 3187
Bso31I GGTCTC 1 cut(s) 3021
BsoBI CYCGRG 2 cut(s) 1178, 2546
Bsp119I TTCGAA 1 cut(s) 1023
Bsp1286I GDGCHC 1 cut(s) 1858
Bsp1407I TGTACA 2 cut(s) 1634, 2439
Bsp1720I GCTNAGC 1 cut(s) 2000
Bsp19I CCATGG 1 cut(s) 2659
BspACI CCGC 4 cut(s) 587, 932, 2527, 3229
BspANI GGCC 6 cut(s) 234, 609, 1062, 1113, 1845, 3187
BspFNI CGCG 1 cut(s) 2529
BspLI GGNNCC 4 cut(s) 538, 1775, 2386, 2425
BspMAI CTGCAG 1 cut(s) 1080
BspMI ACCTGC 2 cut(s) 1273, 1476
BspPI GGATC 8 cut(s) 111, 122, 721, 823, 2128, 2303, 2379, 3281
BspQI GCTCTTC 2 cut(s) 498, 3043
BspT104I TTCGAA 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 2384
BspTNI GGTCTC 1 cut(s) 3021
BsrDI GCAATG 5 cut(s) 2214, 2277, 2560, 2904, 3204
BsrGI TGTACA 2 cut(s) 1634, 2439
BssSI CACGAG 2 cut(s) 44, 1619
BssT1I CCWWGG 5 cut(s) 131, 207, 1201, 1306, 2659
Bst2BI CACGAG 2 cut(s) 44, 1619
Bst2UI CCWGG 3 cut(s) 546, 691, 2369
Bst4CI ACNGT 9 cut(s) 347, 622, 1164, 1214, 1512, 2047, 2437, 2774, 3241
Bst6I CTCTTC 5 cut(s) 498, 857, 1025, 3043, 3120
BstAUI TGTACA 2 cut(s) 1634, 2439
BstBI TTCGAA 1 cut(s) 1023
BstC8I GCNNGC 3 cut(s) 798, 2756, 2890
BstDSI CCRYGG 2 cut(s) 977, 2659
BstENI CCTNNNNNAGG 2 cut(s) 1594, 3294
BstF5I GGATG 6 cut(s) 1156, 1827, 2079, 2809, 3074, 3286
BstFNI CGCG 1 cut(s) 2529
BstH2I RGCGCY 1 cut(s) 2281
BstHHI GCGC 3 cut(s) 1270, 2280, 2531
BstMAI GTCTC 6 cut(s) 203, 261, 1365, 1377, 1409, 3021
BstMWI GCNNNNNNNGC 7 cut(s) 793, 802, 1648, 2453, 2526, 2717, 2898
BstNI CCWGG 3 cut(s) 546, 691, 2369
BstNSI RCATGY 3 cut(s) 1237, 1356, 2825
BstPAI GACNNNNGTC 1 cut(s) 3002
BstSCI CCNGG 4 cut(s) 544, 689, 1686, 2367
BstSFI CTRYAG 4 cut(s) 1076, 1643, 1859, 2956
BstUI CGCG 1 cut(s) 2529
BstV1I GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
BstX2I RGATCY 6 cut(s) 116, 2114, 2308, 2371, 3100, 3286
BstXI CCANNNNNNTGG 2 cut(s) 893, 965
BstYI RGATCY 6 cut(s) 116, 2114, 2308, 2371, 3100, 3286
Bsu36I CCTNAGG 4 cut(s) 428, 474, 581, 834
BsuI GTATCC 1 cut(s) 1749
BsuRI GGCC 6 cut(s) 234, 609, 1062, 1113, 1845, 3187
BtgI CCRYGG 2 cut(s) 977, 2659
BtgZI GCGATG 1 cut(s) 3224
BtrI CACGTC 1 cut(s) 2326
BtsCI GGATG 6 cut(s) 1156, 1827, 2079, 2809, 3074, 3286
BtsIMutI CAGTG 8 cut(s) 228, 531, 638, 1674, 2433, 3011, 3072, 3172
BveI ACCTGC 2 cut(s) 1273, 1476
Cac8I GCNNGC 3 cut(s) 798, 2756, 2890
CaiI CAGNNNCTG 1 cut(s) 761
CfoI GCGC 3 cut(s) 1270, 2280, 2531
Cfr13I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
CpoI CGGWCCG 1 cut(s) 3231
Csp6I GTAC 8 cut(s) 924, 1635, 1764, 2365, 2440, 2626, 3117, 3162
CspCI CAANNNNNGTGG 2 cut(s) 966, 1001
CspI CGGWCCG 1 cut(s) 3231
CviQI GTAC 8 cut(s) 924, 1635, 1764, 2365, 2440, 2626, 3117, 3162
DraI TTTAAA 1 cut(s) 1492
DriI GACNNNNNGTC 1 cut(s) 2782
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 5 cut(s) 498, 857, 1025, 3043, 3120
Eam1105I GACNNNNNGTC 1 cut(s) 2782
EarI CTCTTC 5 cut(s) 498, 857, 1025, 3043, 3120
Eco130I CCWWGG 5 cut(s) 131, 207, 1201, 1306, 2659
Eco31I GGTCTC 1 cut(s) 3021
Eco32I GATATC 1 cut(s) 2538
Eco47I GGWCC 4 cut(s) 320, 1463, 1774, 3231
Eco47III AGCGCT 1 cut(s) 2279
Eco57I CTGAAG 2 cut(s) 881, 3144
Eco81I CCTNAGG 4 cut(s) 428, 474, 581, 834
Eco88I CYCGRG 2 cut(s) 1178, 2546
EcoNI CCTNNNNNAGG 2 cut(s) 1594, 3294
EcoO109I RGGNCCY 2 cut(s) 1463, 1774
EcoRI GAATTC 2 cut(s) 154, 290
EcoRII CCWGG 3 cut(s) 544, 689, 2367
EcoRV GATATC 1 cut(s) 2538
EcoT14I CCWWGG 5 cut(s) 131, 207, 1201, 1306, 2659
ErhI CCWWGG 5 cut(s) 131, 207, 1201, 1306, 2659
FalI AAGNNNNNCTT 6 cut(s) 78, 110, 296, 328, 1073, 1105
FaqI GGGAC 4 cut(s) 51, 1787, 1934, 3157
FauI CCCGC 1 cut(s) 580
FauNDI CATATG 1 cut(s) 2896
Fnu4HI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
FokI GGATG 6 cut(s) 1163, 1814, 2086, 2816, 3061, 3293
Fsp4HI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
FspI TGCGCA 1 cut(s) 1269
GlaI GCGC 3 cut(s) 1269, 2279, 2530
GluI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
GsaI CCCAGC 2 cut(s) 1628, 1844
GsuI CTGGAG 6 cut(s) 243, 1458, 1698, 2127, 2266, 3035
HaeII RGCGCY 1 cut(s) 2281
HaeIII GGCC 6 cut(s) 234, 609, 1062, 1113, 1845, 3187
HapII CCGG 2 cut(s) 1688, 2991
HhaI GCGC 3 cut(s) 1270, 2280, 2531
Hin6I GCGC 3 cut(s) 1268, 2278, 2529
HinP1I GCGC 3 cut(s) 1268, 2278, 2529
HindIII AAGCTT 2 cut(s) 1247, 1727
HpaII CCGG 2 cut(s) 1688, 2991
HphI GGTGA 9 cut(s) 403, 476, 1007, 1910, 1982, 2003, 2479, 2588, 3249
Hpy166II GTNNAC 3 cut(s) 911, 2440, 2770
Hpy8I GTNNAC 3 cut(s) 911, 2440, 2770
HpyAV CCTTC 8 cut(s) 671, 1031, 1588, 2015, 2348, 2412, 2495, 3300
HpyCH4III ACNGT 9 cut(s) 347, 622, 1164, 1214, 1512, 2047, 2437, 2774, 3241
HpyCH4IV ACGT 1 cut(s) 2325
HpyF10VI GCNNNNNNNGC 7 cut(s) 793, 802, 1648, 2453, 2526, 2717, 2898
HpySE526I ACGT 1 cut(s) 2325
HspAI GCGC 3 cut(s) 1268, 2278, 2529
LguI GCTCTTC 2 cut(s) 498, 3043
LmnI GCTCC 8 cut(s) 542, 694, 1054, 1205, 2169, 2285, 2423, 2717
Lsp1109I GCAGC 6 cut(s) 1062, 1084, 1579, 2206, 2532, 2732
LweI GCATC 4 cut(s) 1279, 1836, 2220, 3211
MaeII ACGT 1 cut(s) 2325
MfeI CAATTG 2 cut(s) 178, 1986
MflI RGATCY 6 cut(s) 116, 2114, 2308, 2371, 3100, 3286
MhlI GDGCHC 1 cut(s) 1858
MlsI TGGCCA 1 cut(s) 1062
MluNI TGGCCA 1 cut(s) 1062
MlyI GAGTC 7 cut(s) 33, 178, 978, 2514, 2597, 3127, 3280
MmeI TCCRAC 4 cut(s) 1776, 1914, 2310, 3031
Mox20I TGGCCA 1 cut(s) 1062
MroXI GAANNNNTTC 4 cut(s) 311, 705, 1443, 1670
MscI TGGCCA 1 cut(s) 1062
MseI TTAA 8 cut(s) 237, 491, 572, 746, 962, 1347, 1491, 3180
MslI CAYNNNNRTG 3 cut(s) 416, 2658, 3209
Msp20I TGGCCA 1 cut(s) 1062
MspA1I CMGCKG 3 cut(s) 859, 1075, 1840
MspI CCGG 2 cut(s) 1688, 2991
MspR9I CCNGG 4 cut(s) 546, 691, 1688, 2369
MunI CAATTG 2 cut(s) 178, 1986
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 3 cut(s) 546, 691, 2369
MvnI CGCG 1 cut(s) 2529
MwoI GCNNNNNNNGC 7 cut(s) 793, 802, 1648, 2453, 2526, 2717, 2898
NciI CCSGG 1 cut(s) 1688
NcoI CCATGG 1 cut(s) 2659
NdeI CATATG 1 cut(s) 2896
NlaIV GGNNCC 4 cut(s) 538, 1775, 2386, 2425
NmuCI GTSAC 7 cut(s) 1573, 2594, 2774, 2935, 2948, 3034, 3235
NsbI TGCGCA 1 cut(s) 1269
NspI RCATGY 3 cut(s) 1237, 1356, 2825
NspV TTCGAA 1 cut(s) 1023
PaeR7I CTCGAG 1 cut(s) 1178
PasI CCCWGGG 1 cut(s) 690
PciI ACATGT 2 cut(s) 1233, 2821
PciSI GCTCTTC 2 cut(s) 498, 3043
PctI GAATGC 1 cut(s) 1224
PdmI GAANNNNTTC 4 cut(s) 311, 705, 1443, 1670
PfeI GAWTC 7 cut(s) 667, 1455, 1711, 1937, 2078, 2245, 2608
PflFI GACNNNGTC 1 cut(s) 3032
PflMI CCANNNNNTGG 1 cut(s) 3013
PfoI TCCNGGA 1 cut(s) 1686
PkrI GCNGC 6 cut(s) 1074, 1077, 1569, 2221, 2522, 2722
PleI GAGTC 7 cut(s) 33, 177, 977, 2513, 2597, 3127, 3279
PpsI GAGTC 7 cut(s) 33, 177, 977, 2513, 2597, 3127, 3279
PpuMI RGGWCCY 2 cut(s) 1463, 1774
PscI ACATGT 2 cut(s) 1233, 2821
PshAI GACNNNNGTC 1 cut(s) 3002
Psp5II RGGWCCY 2 cut(s) 1463, 1774
Psp6I CCWGG 3 cut(s) 544, 689, 2367
PspFI CCCAGC 2 cut(s) 1624, 1840
PspGI CCWGG 3 cut(s) 544, 689, 2367
PspN4I GGNNCC 4 cut(s) 538, 1775, 2386, 2425
PspPI GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
PspPPI RGGWCCY 2 cut(s) 1463, 1774
PstI CTGCAG 1 cut(s) 1080
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 6 cut(s) 116, 2114, 2308, 2371, 3100, 3286
PsyI GACNNNGTC 1 cut(s) 3032
PvuII CAGCTG 3 cut(s) 859, 1075, 1840
RsaI GTAC 8 cut(s) 925, 1636, 1765, 2366, 2441, 2627, 3118, 3163
RsaNI GTAC 8 cut(s) 924, 1635, 1764, 2365, 2440, 2626, 3117, 3162
RseI CAYNNNNRTG 3 cut(s) 416, 2658, 3209
Rsr2I CGGWCCG 1 cut(s) 3231
RsrII CGGWCCG 1 cut(s) 3231
SapI GCTCTTC 2 cut(s) 498, 3043
SaqAI TTAA 8 cut(s) 237, 491, 572, 746, 962, 1347, 1491, 3180
SatI GCNGC 6 cut(s) 1073, 1076, 1568, 2220, 2521, 2721
Sau96I GGNCC 7 cut(s) 320, 608, 1112, 1463, 1774, 1843, 3231
ScaI AGTACT 1 cut(s) 1765
SchI GAGTC 7 cut(s) 33, 178, 978, 2514, 2597, 3127, 3280
ScrFI CCNGG 4 cut(s) 546, 691, 1688, 2369
SduI GDGCHC 1 cut(s) 1858
SfaNI GCATC 4 cut(s) 1279, 1836, 2220, 3211
SfcI CTRYAG 4 cut(s) 1076, 1643, 1859, 2956
Sfr274I CTCGAG 1 cut(s) 1178
SfuI TTCGAA 1 cut(s) 1023
SinI GGWCC 4 cut(s) 320, 1463, 1774, 3231
SlaI CTCGAG 1 cut(s) 1178
SmiMI CAYNNNNRTG 3 cut(s) 416, 2658, 3209
SmlI CTYRAG 5 cut(s) 200, 1178, 1547, 2486, 3143
SmoI CTYRAG 5 cut(s) 200, 1178, 1547, 2486, 3143
SpeI ACTAGT 1 cut(s) 2983
SsiI CCGC 4 cut(s) 587, 932, 2527, 3229
SspI AATATT 3 cut(s) 1333, 1876, 2569
StyD4I CCNGG 4 cut(s) 544, 689, 1686, 2367
StyI CCWWGG 5 cut(s) 131, 207, 1201, 1306, 2659
TaaI ACNGT 9 cut(s) 347, 622, 1164, 1214, 1512, 2047, 2437, 2774, 3241
TaiI ACGT 1 cut(s) 2328
TaqI TCGA 6 cut(s) 592, 1023, 1179, 2514, 2606, 3087
TatI WGTACW 5 cut(s) 1634, 1763, 2439, 2625, 3116
TfiI GAWTC 7 cut(s) 667, 1455, 1711, 1937, 2078, 2245, 2608
Tru1I TTAA 8 cut(s) 237, 491, 572, 746, 962, 1347, 1491, 3180
Tru9I TTAA 8 cut(s) 237, 491, 572, 746, 962, 1347, 1491, 3180
TscAI CASTG 8 cut(s) 235, 538, 638, 1681, 2440, 3018, 3072, 3172
TseFI GTSAC 7 cut(s) 1573, 2594, 2774, 2935, 2948, 3034, 3235
TseI GCWGC 6 cut(s) 1072, 1075, 1567, 2219, 2520, 2720
Tsp45I GTSAC 7 cut(s) 1573, 2594, 2774, 2935, 2948, 3034, 3235
TspGWI ACGGA 2 cut(s) 2182, 3223
TspRI CASTG 8 cut(s) 235, 538, 638, 1681, 2440, 3018, 3072, 3172
Tth111I GACNNNGTC 1 cut(s) 3032
Van91I CCANNNNNTGG 1 cut(s) 3013
VpaK11BI GGWCC 4 cut(s) 320, 1463, 1774, 3231
XagI CCTNNNNNAGG 2 cut(s) 1594, 3294
XapI RAATTY 8 cut(s) 154, 290, 595, 1127, 1188, 1902, 1974, 2086
XbaI TCTAGA 2 cut(s) 364, 2683
XceI RCATGY 3 cut(s) 1237, 1356, 2825
XcmI CCANNNNNNNNNTGG 1 cut(s) 2169
XhoI CTCGAG 1 cut(s) 1178
XmnI GAANNNNTTC 4 cut(s) 311, 705, 1443, 1670
ZrmI AGTACT 1 cut(s) 1765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.