Rorug05G0397100

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000148
Physical Location & Seq
Reverse (-)
54573760 .. 54579746
5987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0397100.1

Sequence Viewer

Length: 2922 bp
ATGTCTAATCGCGGCGGTTCATCCGGTGGTCGCGGCGGCGGAGGCCGCGGCCGAAGAGGAAATCCCGGCGGTCAGGCCAGAGGCTCAGGCTACACTCCGGCGCCCGCGCCTGCTCCAGCTCCGGCGCCGGCTCGTGCTCAGCCGCCTGCTGTGGCCTCGTCGAGCACGGTACCGGCGCCGGCTCGTACTCATCAGCCTGCTGTGGCCTCGTCGAGCAGGGCTCATGCTCCGGCTCCGGCGCCGGCTCCTGCTCCGGCTCCGGCACCTGTTTCATCGGCGGCGGCGGCTTCGCTGGCGAGTGAGATGGAAGAGAAGCTTGCATTGGCTCCGTCGTCATCCAAGGCCGTGAGGTTTCCAGTGAGGCCGGGGTTTGGTAGAGCTGGTCAAAGAGTCCAAGTCCGAGCCAATCATTTTCTGGTCGAGGTTGGGGAAAGAGATCTGCATCACTATGATGTATCAATTAGCCCTGAAGTTGCATCAAAGAAGATAAACAGAGATGTAATCAACCAGCTGGTTCAGATGTACTCTGAGTCTCACTTGGGAAAGAGAATGCCAGCATATGATGGCATGAAGAGTATCTACACTGCCGGGCCATTGCCCTTCTCTTCAAAGGAGTTTGTGGTCAAGCTACCTGAGAGAGAAGGTCGAGCTGCATCTGCATCAAGAAGGAAAGATAGAGACTTCAAGGTCTGCATTAAGTTGGCAAACAAGCCTGACCTGTATCAACTGCAGCAGTTCTTACGTGGAGGACTACACGAAACACCGCAAGAGACCATCCAAGTTCTTGATGTTGTTCTTAGAGCTGCACCATCACAGAAATACTGTGTGGTTGGTAGGTCATTTTTTAGCCCTGATCTGGGTCCTCGAGGTGACCTTGAATTTGGCTTGGAATACTGGAGAGGCTTTTACCAAAGCCTAAGGCCAACTCAGTTTGGTCTATCCCTAAATATTGATATCTCAGCAAGGGCTTTTTATGATCCGATTCTGGTGACAGAGTTTATCAAGAAACATTTTGGGTACAGAGATTTATCCAGGGGTCTGTCAGATCGTGATTGTCTGAAGCTGAAGAAAAATCTGAAAGGAATCAAGGTAACTATGAGCTGTGGAGGCAGCAGAAGTTATCGAATTACTGGAGTATCTGTGCAGCCACTAAGCCAGTTAACGTTCACTCTTGAGGATAATCAAACCAGAGTATCAGTGGTTCAGTATTATCGTGAGAAGTACAACATTAGCCTAAAACAGGTGGCTTTGCCTGCCCTTCAATCTGGCAATGATTCAAGGCCTGCTTATTTGCCCATGGAGCTCGCTTCAATTGTTCCTGGGCAGAGATACACGAAAAGACTCAATGAAAAGCAAGTGACAAACGTGCTGAGAGCAACTTGCCAACGGCCCCGGGAGAGGGAATCCAATATACTGAAGATGGTGAAGCACAATAATTACAAGGGAGAGGATCTTGTAAACAAAGAATTTGGAATACAAGTTAAAGAACAAATGGCACTGGTGGATGCTAGAGTATTACAACCTCCAATGCTTAAGTATCATGAGAGTGGTCGTGAAACAAAGGATACTCCCAGGATGGGTGCATGGAACATGATCAATAAGAAAATGGTTAATGGTGCCAAAGTGGATTTCTGGACTTTTGTCAATTTCTCTCGATTACGTGAAGATTTTAACTATCACTTTTGTGAGGATCTCGTCTCTATGTGCAACAGTAAAGGGGTGGAGTTCCATTCCGAGCCTTTACTTCCTGTACAGTCGGCTCATCCTAATCAAATTGAGAGGGTTCTGAGAGATATTCACAAGCAGTCAGTCCAGAGACTTAAGGAGATGGGACAAGCCAGCAAACATCTTCAGTTGTTGATTATCATCTTGCCTGATGTCAAAGGATCTTATGGTCCCATTAAAAGAATTTGTGAAACAGAGCTTGGAATTGTCTCCCAGTGCTGTAACCCTAAGCAAGCACAAAAGATTAGTAAGCAGTACCTTGAAAATCTGTCTCTTAAGATTAATGTCAAGGTTGGTGGTAGAAACACAGTCTTAAATGATGCCATTCAGAGGAGGATTCCTCTCGTCAGTGATCGTCCTACAATCATCCTTGGAGCTGATGTCACTCATCCACAAGCTGGGGAAGACAGTAGCCCATCTATAGCAGCAGTGGTTGCCTCAATGGACTGGCCAGAGGTCTCCAAGTATAGAGGAATCCTGTCAGCACAGAATCATCGTCAAGAAATCATCCAAGATCTTTACAAATTAACTCACGATGAGAAAAGAGGGCCGGTTCATTCAGGAATGATCAGAGAACTTTTCATTGCATTCCGAAGATCGACTGGCCAAAAGCCGCACAGAATCATCTTCTATAGGGATGGGGTGAGTGAAGGGCAATTTAGCCAAGTTCTGCTCTATGAGATGGATGCGATACGGAAGGCCTGTGCTTCTTTGGAGGATGGGTATATGCCACCAGTTACTTTTGTTGTGGTGCAGAAAAGGCATCACACGCGCCTTTTCCCTGCTGACAACCAGACAGATAGGAGTGGGAATATTCAACCAGGTACTGTGGTGGATACCAAGATATGCCATCCAACAGAATTTGATTTCTATCTTAATAGCCATGCAGGAATTCAGGGAACAAGTCGCCCAGCTCATTATCATGTCCTGTTTGATGAGAACAACTTCACTGCTGATGGCTTGCAGATTCTCACCAACAATCTGTGCTACACATATGCAAGGTGCACCCGCTCAGTTTCCATAGTGCCTCCTGCCTATTATGCGCATCTAGCTGCGTTTAGAGCACGCTATTACATTGAGGGTGATTCATCAGATGGTGCTTCTTCAGTTGGCAGAGGCAGCGGTGGCAGTGCTGCACAAACCCAGGTCCTTCCAAATGTGCATGAGTATGTCAAGGATGTGATGTTTTTCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

973

Amino Acids

108.07

Weight (kDa)

9.54

Isoelectric Point (pI)

51.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 129 - 266 2.9e-37 N-terminal domain of argonaute
ArgoL1 PF08699 277 - 325 3.4e-18 Argonaute linker 1 domain
PAZ PF02170 353 - 456 6e-26 PAZ domain
ArgoL2 PF16488 466 - 513 1.2e-13 Argonaute linker 2 domain
ArgoMid PF16487 523 - 601 3.2e-09 Mid domain of argonaute
Piwi PF02171 620 - 934 1e-107 Piwi domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 686
AbsI CCTCGAGG 1 cut(s) 864
Acc16I TGCGCA 1 cut(s) 2769
Acc65I GGTACC 1 cut(s) 169
AccB1I GGYRCC 7 cut(s) 100, 124, 169, 175, 238, 262, 1616
AccB7I CCANNNNNTGG 1 cut(s) 2123
AccBSI CCGCTC 1 cut(s) 2736
AccII CGCG 5 cut(s) 12, 33, 48, 107, 2497
AclI AACGTT 1 cut(s) 1163
AclWI GGATC 4 cut(s) 971, 1458, 1698, 1894
AcoI YGGCCR 3 cut(s) 49, 2174, 2329
AcsI RAATTY 5 cut(s) 878, 1466, 1908, 2585, 2616
AcuI CTGAAG 6 cut(s) 489, 1079, 1085, 1436, 1835, 2814
AcyI GRCGYC 4 cut(s) 101, 125, 176, 239
AfaI GTAC 8 cut(s) 171, 187, 524, 1019, 1223, 1752, 1982, 2551
AfiI CCNNNNNNNGG 9 cut(s) 371, 856, 857, 1240, 1398, 1399, 1577, 2055, 2123
AflII CTTAAG 3 cut(s) 1532, 1820, 2000
AgsI TTSAA 8 cut(s) 609, 685, 878, 1262, 1278, 1311, 1988, 2543
AjnI CCWGG 5 cut(s) 1031, 1318, 1571, 2545, 2868
AjuI GAANNNNNNNTTGG 6 cut(s) 305, 337, 1613, 1645, 1908, 1940
AleI CACNNNNGTG 1 cut(s) 1683
Alw21I GWGCWC 5 cut(s) 139, 167, 1305, 2732, 2791
Alw26I GTCTC 8 cut(s) 537, 672, 764, 1702, 1810, 1939, 2001, 2188
Alw44I GTGCAC 1 cut(s) 2728
AlwI GGATC 4 cut(s) 971, 1458, 1698, 1894
AlwNI CAGNNNCTG 1 cut(s) 2552
Ama87I CYCGRG 2 cut(s) 864, 1392
ApaLI GTGCAC 1 cut(s) 2728
ApeKI GCWGC 9 cut(s) 650, 730, 803, 1110, 1144, 2150, 2777, 2844, 2858
ApoI RAATTY 5 cut(s) 878, 1466, 1908, 2585, 2616
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AseI ATTAAT 1 cut(s) 2007
Asp700I GAANNNNTTC 1 cut(s) 2669
Asp718I GGTACC 1 cut(s) 169
AspLEI GCGC 7 cut(s) 103, 109, 127, 178, 241, 2499, 2770
AspS9I GGNCC 6 cut(s) 590, 860, 1389, 1895, 2273, 2872
AsuC2I CCSGG 5 cut(s) 66, 366, 589, 1393, 1394
AsuHPI GGTGA 6 cut(s) 881, 1000, 1435, 2380, 2689, 2819
AvaI CYCGRG 2 cut(s) 864, 1392
AvaII GGWCC 3 cut(s) 860, 1895, 2872
AxyI CCTNAGG 1 cut(s) 917
BaeGI GKGCMC 1 cut(s) 2732
BaeI ACNNNNGTAYC 6 cut(s) 1119, 1152, 1176, 1209, 2541, 2574
BalI TGGCCA 2 cut(s) 2176, 2331
BanI GGYRCC 7 cut(s) 100, 124, 169, 175, 238, 262, 1616
BanII GRGCYC 2 cut(s) 223, 1305
BarI GAAGNNNNNNTAC 2 cut(s) 563, 595
BauI CACGAG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 2136
Bbv12I GWGCWC 5 cut(s) 139, 167, 1305, 2732, 2791
BbvI GCAGC 9 cut(s) 637, 742, 790, 1122, 1156, 2162, 2764, 2845, 2856
BceAI ACGGC 2 cut(s) 329, 1403
BciT130I CCWGG 5 cut(s) 1033, 1320, 1573, 2547, 2870
BciVI GTATCC 2 cut(s) 1558, 2554
BclI TGATCA 2 cut(s) 1593, 2292
BcnI CCSGG 5 cut(s) 66, 366, 589, 1393, 1394
BcoDI GTCTC 8 cut(s) 537, 672, 764, 1702, 1810, 1939, 2001, 2188
BfaI CTAG 2 cut(s) 1509, 2774
BfmI CTRYAG 3 cut(s) 728, 2145, 2356
BfoI RGCGCY 4 cut(s) 104, 128, 179, 242
BfrI CTTAAG 3 cut(s) 1532, 1820, 2000
BfuI GTATCC 2 cut(s) 1558, 2554
BglII AGATCT 2 cut(s) 436, 2239
BlpI GCTNAGC 1 cut(s) 138
Bme18I GGWCC 3 cut(s) 860, 1895, 2872
BmeT110I CYCGRG 2 cut(s) 864, 1392
BmgT120I GGNCC 6 cut(s) 590, 860, 1389, 1895, 2273, 2872
BmrI ACTGGG 1 cut(s) 1933
BmsI GCATC 9 cut(s) 451, 485, 662, 668, 1495, 2035, 2401, 2497, 2779
BmuI ACTGGG 1 cut(s) 1933
BoxI GACNNNNGTC 1 cut(s) 1640
BpiI GAAGAC 1 cut(s) 2136
BplI GAGNNNNNCTC 4 cut(s) 205, 237, 2050, 2082
BpmI CTGGAG 3 cut(s) 99, 916, 1152
Bpu10I CCTNAGC 2 cut(s) 85, 1953
Bpu1102I GCTNAGC 1 cut(s) 138
BpuEI CTTGAG 1 cut(s) 1193
BpuMI CCSGG 5 cut(s) 66, 366, 589, 1393, 1394
BsaAI YACGTR 2 cut(s) 743, 1661
BsaBI GATNNNNATC 3 cut(s) 441, 1865, 2595
BsaHI GRCGYC 4 cut(s) 101, 125, 176, 239
BsaI GGTCTC 2 cut(s) 764, 2188
BsaWI WCCGGW 1 cut(s) 23
BsaXI ACNNNNNCTCC 6 cut(s) 1125, 1155, 2091, 2121, 2432, 2462
Bsc4I CCNNNNNNNGG 9 cut(s) 371, 856, 857, 1240, 1398, 1399, 1577, 2055, 2123
Bse118I RCCGGY 5 cut(s) 127, 172, 178, 241, 2275
Bse1I ACTGG 9 cut(s) 356, 899, 1135, 1156, 1503, 1939, 2177, 2332, 2459
Bse21I CCTNAGG 1 cut(s) 917
Bse3DI GCAATG 3 cut(s) 593, 1276, 2307
Bse8I GATNNNNATC 3 cut(s) 441, 1865, 2595
BseBI CCWGG 5 cut(s) 1033, 1320, 1573, 2547, 2870
BseJI GATNNNNATC 3 cut(s) 441, 1865, 2595
BseLI CCNNNNNNNGG 9 cut(s) 371, 856, 857, 1240, 1398, 1399, 1577, 2055, 2123
BseMI GCAATG 3 cut(s) 593, 1276, 2307
BseMII CTCAG 9 cut(s) 99, 152, 519, 624, 941, 972, 1361, 1778, 2751
BseNI ACTGG 9 cut(s) 356, 899, 1135, 1156, 1503, 1939, 2177, 2332, 2459
BseRI GAGGAG 1 cut(s) 2071
BseSI GKGCMC 1 cut(s) 2732
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 9 cut(s) 637, 742, 790, 1122, 1156, 2162, 2764, 2845, 2856
BseYI CCCAGC 2 cut(s) 2123, 2635
BsgI GTGCAG 4 cut(s) 789, 1163, 2498, 2844
Bsh1236I CGCG 5 cut(s) 12, 33, 48, 107, 2497
Bsh1285I CGRYCG 1 cut(s) 52
BshNI GGYRCC 7 cut(s) 100, 124, 169, 175, 238, 262, 1616
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 5 cut(s) 139, 167, 1305, 2732, 2791
BsiHKCI CYCGRG 2 cut(s) 864, 1392
BslFI GGGAC 2 cut(s) 1845, 1881
BslI CCNNNNNNNGG 9 cut(s) 371, 856, 857, 1240, 1398, 1399, 1577, 2055, 2123
BsmAI GTCTC 8 cut(s) 537, 672, 764, 1702, 1810, 1939, 2001, 2188
BsmBI CGTCTC 1 cut(s) 1702
BsmFI GGGAC 2 cut(s) 1845, 1881
BsmI GAATGC 2 cut(s) 555, 2312
Bso31I GGTCTC 2 cut(s) 764, 2188
BsoBI CYCGRG 2 cut(s) 864, 1392
Bsp1286I GDGCHC 6 cut(s) 139, 167, 223, 1305, 2732, 2791
Bsp1407I TGTACA 1 cut(s) 1750
Bsp1720I GCTNAGC 1 cut(s) 138
Bsp19I CCATGG 1 cut(s) 1296
BspCNI CTCAG 9 cut(s) 98, 151, 520, 625, 940, 971, 1362, 1779, 2750
BspFNI CGCG 5 cut(s) 12, 33, 48, 107, 2497
BspHI TCATGA 1 cut(s) 1540
BspMAI CTGCAG 1 cut(s) 732
BspPI GGATC 4 cut(s) 971, 1458, 1698, 1894
BspT107I GGYRCC 7 cut(s) 100, 124, 169, 175, 238, 262, 1616
BspTI CTTAAG 3 cut(s) 1532, 1820, 2000
BspTNI GGTCTC 2 cut(s) 764, 2188
BsrBI CCGCTC 1 cut(s) 2736
BsrDI GCAATG 3 cut(s) 593, 1276, 2307
BsrFI RCCGGY 5 cut(s) 127, 172, 178, 241, 2275
BsrGI TGTACA 1 cut(s) 1750
BsrI ACTGG 9 cut(s) 356, 899, 1135, 1156, 1503, 1939, 2177, 2332, 2459
BssAI RCCGGY 5 cut(s) 127, 172, 178, 241, 2275
BssNI GRCGYC 4 cut(s) 101, 125, 176, 239
BssSI CACGAG 1 cut(s) 132
BssT1I CCWWGG 3 cut(s) 339, 1296, 2095
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 5 cut(s) 1033, 1320, 1573, 2547, 2870
Bst4CI ACNGT 7 cut(s) 169, 824, 1712, 1755, 2035, 2135, 2554
Bst6I CTCTTC 4 cut(s) 49, 303, 566, 610
BstACI GRCGYC 4 cut(s) 101, 125, 176, 239
BstAFI CTTAAG 3 cut(s) 1532, 1820, 2000
BstAPI GCANNNNNTGC 1 cut(s) 2159
BstAUI TGTACA 1 cut(s) 1750
BstBAI YACGTR 2 cut(s) 743, 1661
BstDSI CCRYGG 2 cut(s) 46, 1296
BstEII GGTNACC 1 cut(s) 869
BstENI CCTNNNNNAGG 1 cut(s) 1238
BstFNI CGCG 5 cut(s) 12, 33, 48, 107, 2497
BstH2I RGCGCY 4 cut(s) 104, 128, 179, 242
BstHHI GCGC 7 cut(s) 103, 109, 127, 178, 241, 2499, 2770
BstMAI GTCTC 8 cut(s) 537, 672, 764, 1702, 1810, 1939, 2001, 2188
BstMCI CGRYCG 1 cut(s) 52
BstNI CCWGG 5 cut(s) 1033, 1320, 1573, 2547, 2870
BstPAI GACNNNNGTC 1 cut(s) 1640
BstPI GGTNACC 1 cut(s) 869
BstSFI CTRYAG 3 cut(s) 728, 2145, 2356
BstSLI GKGCMC 1 cut(s) 2732
BstUI CGCG 5 cut(s) 12, 33, 48, 107, 2497
BstV1I GCAGC 9 cut(s) 637, 742, 790, 1122, 1156, 2162, 2764, 2845, 2856
BstV2I GAAGAC 1 cut(s) 2136
BstX2I RGATCY 5 cut(s) 436, 1450, 1690, 1886, 2239
BstYI RGATCY 5 cut(s) 436, 1450, 1690, 1886, 2239
BstZI CGGCCG 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 917
BsuI GTATCC 2 cut(s) 1558, 2554
BtgI CCRYGG 2 cut(s) 46, 1296
BtsI GCAGTG 4 cut(s) 582, 2160, 2673, 2860
BtsIMutI CAGTG 9 cut(s) 363, 582, 1203, 1496, 1946, 2080, 2160, 2673, 2860
CaiI CAGNNNCTG 1 cut(s) 2552
CciI TCATGA 1 cut(s) 1540
CfoI GCGC 7 cut(s) 103, 109, 127, 178, 241, 2499, 2770
Cfr10I RCCGGY 5 cut(s) 127, 172, 178, 241, 2275
Cfr13I GGNCC 6 cut(s) 590, 860, 1389, 1895, 2273, 2872
Cfr42I CCGCGG 1 cut(s) 49
Cfr9I CCCGGG 1 cut(s) 1392
CsiI ACCWGGT 1 cut(s) 2545
Csp6I GTAC 8 cut(s) 170, 186, 523, 1018, 1222, 1751, 1981, 2550
CspCI CAANNNNNGTGG 2 cut(s) 2002, 2037
CviAII CATG 9 cut(s) 224, 568, 1297, 1541, 1584, 1591, 2609, 2648, 2888
CviQI GTAC 8 cut(s) 170, 186, 523, 1018, 1222, 1751, 1981, 2550
DinI GGCGCC 4 cut(s) 102, 126, 177, 240
DrdI GACNNNNNNGTC 1 cut(s) 686
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 3 cut(s) 49, 2174, 2329
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 4 cut(s) 49, 303, 566, 610
EarI CTCTTC 4 cut(s) 49, 303, 566, 610
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 1 cut(s) 1303
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 3 cut(s) 339, 1296, 2095
Eco147I AGGCCT 2 cut(s) 1282, 2426
Eco24I GRGCYC 2 cut(s) 223, 1305
Eco31I GGTCTC 2 cut(s) 764, 2188
Eco32I GATATC 1 cut(s) 955
Eco47I GGWCC 3 cut(s) 860, 1895, 2872
Eco52I CGGCCG 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 1303
Eco57I CTGAAG 6 cut(s) 489, 1079, 1085, 1436, 1835, 2814
Eco81I CCTNAGG 1 cut(s) 917
Eco88I CYCGRG 2 cut(s) 864, 1392
Eco91I GGTNACC 1 cut(s) 869
EcoICRI GAGCTC 1 cut(s) 1303
EcoNI CCTNNNNNAGG 1 cut(s) 1238
EcoO109I RGGNCCY 2 cut(s) 860, 2872
EcoO65I GGTNACC 1 cut(s) 869
EcoRI GAATTC 1 cut(s) 2616
EcoRII CCWGG 5 cut(s) 1031, 1318, 1571, 2545, 2868
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 3 cut(s) 339, 1296, 2095
EcoT38I GRGCYC 2 cut(s) 223, 1305
EgeI GGCGCC 4 cut(s) 102, 126, 177, 240
EheI GGCGCC 4 cut(s) 102, 126, 177, 240
ErhI CCWWGG 3 cut(s) 339, 1296, 2095
Esp3I CGTCTC 1 cut(s) 1702
FaeI CATG 9 cut(s) 227, 571, 1300, 1544, 1587, 1594, 2612, 2651, 2891
FalI AAGNNNNNCTT 4 cut(s) 300, 332, 1270, 1302
FaqI GGGAC 2 cut(s) 1845, 1881
FatI CATG 9 cut(s) 223, 567, 1296, 1540, 1583, 1590, 2608, 2647, 2887
FauI CCCGC 2 cut(s) 112, 2741
FauNDI CATATG 2 cut(s) 559, 2719
FbaI TGATCA 2 cut(s) 1593, 2292
FriOI GRGCYC 2 cut(s) 223, 1305
FspAI RTGCGCAY 1 cut(s) 2769
FspBI CTAG 2 cut(s) 1509, 2774
FspI TGCGCA 1 cut(s) 2769
GlaI GCGC 7 cut(s) 102, 108, 126, 177, 240, 2498, 2769
GsaI CCCAGC 2 cut(s) 2127, 2639
GsuI CTGGAG 3 cut(s) 99, 916, 1152
HaeII RGCGCY 4 cut(s) 104, 128, 179, 242
HhaI GCGC 7 cut(s) 103, 109, 127, 178, 241, 2499, 2770
Hin1I GRCGYC 4 cut(s) 101, 125, 176, 239
Hin1II CATG 9 cut(s) 227, 571, 1300, 1544, 1587, 1594, 2612, 2651, 2891
Hin6I GCGC 7 cut(s) 101, 107, 125, 176, 239, 2497, 2768
HinP1I GCGC 7 cut(s) 101, 107, 125, 176, 239, 2497, 2768
HincII GTYRAC 1 cut(s) 1161
HindII GTYRAC 1 cut(s) 1161
HindIII AAGCTT 1 cut(s) 314
HpaI GTTAAC 1 cut(s) 1161
HphI GGTGA 6 cut(s) 881, 1000, 1435, 2380, 2689, 2819
Hpy166II GTNNAC 4 cut(s) 1161, 1167, 1459, 2730
Hpy8I GTNNAC 4 cut(s) 1161, 1167, 1459, 2730
Hpy99I CGWCG 3 cut(s) 163, 214, 334
HpyAV CCTTC 7 cut(s) 610, 635, 660, 1268, 2369, 2416, 2885
HpyCH4III ACNGT 7 cut(s) 169, 824, 1712, 1755, 2035, 2135, 2554
HpyCH4IV ACGT 4 cut(s) 742, 1163, 1365, 1660
HpySE526I ACGT 4 cut(s) 742, 1163, 1365, 1660
Hsp92I GRCGYC 4 cut(s) 101, 125, 176, 239
Hsp92II CATG 9 cut(s) 227, 571, 1300, 1544, 1587, 1594, 2612, 2651, 2891
HspAI GCGC 7 cut(s) 101, 107, 125, 176, 239, 2497, 2768
KasI GGCGCC 4 cut(s) 100, 124, 175, 238
KpnI GGTACC 1 cut(s) 173
KroI GCCGGC 3 cut(s) 127, 178, 241
KroNI GCCGGC 3 cut(s) 129, 180, 243
Ksp22I TGATCA 2 cut(s) 1593, 2292
KspAI GTTAAC 1 cut(s) 1161
KspI CCGCGG 1 cut(s) 49
Lsp1109I GCAGC 9 cut(s) 637, 742, 790, 1122, 1156, 2162, 2764, 2845, 2856
LweI GCATC 9 cut(s) 451, 485, 662, 668, 1495, 2035, 2401, 2497, 2779
MabI ACCWGGT 1 cut(s) 2545
MaeI CTAG 2 cut(s) 1509, 2774
MaeII ACGT 4 cut(s) 742, 1163, 1365, 1660
MaeIII GTNAC 7 cut(s) 869, 988, 1090, 1357, 1946, 2107, 2461
MbiI CCGCTC 1 cut(s) 2736
MfeI CAATTG 1 cut(s) 1311
MflI RGATCY 5 cut(s) 436, 1450, 1690, 1886, 2239
MhlI GDGCHC 6 cut(s) 139, 167, 223, 1305, 2732, 2791
MlsI TGGCCA 2 cut(s) 2176, 2331
MluNI TGGCCA 2 cut(s) 2176, 2331
Mly113I GGCGCC 4 cut(s) 101, 125, 176, 239
MlyI GAGTC 3 cut(s) 399, 539, 1335
MmeI TCCRAC 1 cut(s) 2603
Mox20I TGGCCA 2 cut(s) 2176, 2331
MroNI GCCGGC 3 cut(s) 127, 178, 241
MroXI GAANNNNTTC 1 cut(s) 2669
MscI TGGCCA 2 cut(s) 2176, 2331
MslI CAYNNNNRTG 6 cut(s) 447, 450, 1545, 1683, 2646, 2892
Msp20I TGGCCA 2 cut(s) 2176, 2331
MspA1I CMGCKG 3 cut(s) 48, 511, 2847
MspCI CTTAAG 3 cut(s) 1532, 1820, 2000
MunI CAATTG 1 cut(s) 1311
Mva1269I GAATGC 2 cut(s) 555, 2312
MvaI CCWGG 5 cut(s) 1033, 1320, 1573, 2547, 2870
MvnI CGCG 5 cut(s) 12, 33, 48, 107, 2497
NaeI GCCGGC 3 cut(s) 129, 180, 243
NarI GGCGCC 4 cut(s) 101, 125, 176, 239
NciI CCSGG 5 cut(s) 66, 366, 589, 1393, 1394
NcoI CCATGG 1 cut(s) 1296
NdeI CATATG 2 cut(s) 559, 2719
NgoMIV GCCGGC 3 cut(s) 127, 178, 241
NlaIII CATG 9 cut(s) 227, 571, 1300, 1544, 1587, 1594, 2612, 2651, 2891
NmuCI GTSAC 4 cut(s) 869, 988, 1357, 2107
NsbI TGCGCA 1 cut(s) 2769
OliI CACNNNNGTG 1 cut(s) 1683
PaeR7I CTCGAG 1 cut(s) 864
PagI TCATGA 1 cut(s) 1540
PceI AGGCCT 2 cut(s) 1282, 2426
PctI GAATGC 2 cut(s) 555, 2312
PdiI GCCGGC 3 cut(s) 129, 180, 243
PdmI GAANNNNTTC 1 cut(s) 2669
PflMI CCANNNNNTGG 1 cut(s) 2123
PleI GAGTC 3 cut(s) 398, 538, 1335
PluTI GGCGCC 4 cut(s) 104, 128, 179, 242
PpsI GAGTC 3 cut(s) 398, 538, 1335
Ppu21I YACGTR 2 cut(s) 743, 1661
PpuMI RGGWCCY 2 cut(s) 860, 2872
PshAI GACNNNNGTC 1 cut(s) 1640
PshBI ATTAAT 1 cut(s) 2007
Psp124BI GAGCTC 1 cut(s) 1305
Psp1406I AACGTT 1 cut(s) 1163
Psp5II RGGWCCY 2 cut(s) 860, 2872
Psp6I CCWGG 5 cut(s) 1031, 1318, 1571, 2545, 2868
PspEI GGTNACC 1 cut(s) 869
PspFI CCCAGC 2 cut(s) 2123, 2635
PspGI CCWGG 5 cut(s) 1031, 1318, 1571, 2545, 2868
PspPI GGNCC 6 cut(s) 590, 860, 1389, 1895, 2273, 2872
PspPPI RGGWCCY 2 cut(s) 860, 2872
PspXI VCTCGAGB 1 cut(s) 864
PsrI GAACNNNNNNTAC 2 cut(s) 1185, 1217
PstI CTGCAG 1 cut(s) 732
PstNI CAGNNNCTG 1 cut(s) 2552
PsuI RGATCY 5 cut(s) 436, 1450, 1690, 1886, 2239
PvuII CAGCTG 1 cut(s) 511
RsaI GTAC 8 cut(s) 171, 187, 524, 1019, 1223, 1752, 1982, 2551
RsaNI GTAC 8 cut(s) 170, 186, 523, 1018, 1222, 1751, 1981, 2550
RseI CAYNNNNRTG 6 cut(s) 447, 450, 1545, 1683, 2646, 2892
SacI GAGCTC 1 cut(s) 1305
SacII CCGCGG 1 cut(s) 49
Sau96I GGNCC 6 cut(s) 590, 860, 1389, 1895, 2273, 2872
SchI GAGTC 3 cut(s) 399, 539, 1335
SduI GDGCHC 6 cut(s) 139, 167, 223, 1305, 2732, 2791
SexAI ACCWGGT 1 cut(s) 2545
SfaNI GCATC 9 cut(s) 451, 485, 662, 668, 1495, 2035, 2401, 2497, 2779
SfcI CTRYAG 3 cut(s) 728, 2145, 2356
SfoI GGCGCC 4 cut(s) 102, 126, 177, 240
Sfr274I CTCGAG 1 cut(s) 864
Sfr303I CCGCGG 1 cut(s) 49
SgrBI CCGCGG 1 cut(s) 49
SinI GGWCC 3 cut(s) 860, 1895, 2872
SlaI CTCGAG 1 cut(s) 864
SmaI CCCGGG 1 cut(s) 1394
SmiMI CAYNNNNRTG 6 cut(s) 447, 450, 1545, 1683, 2646, 2892
SmlI CTYRAG 5 cut(s) 864, 1172, 1532, 1820, 2000
SmoI CTYRAG 5 cut(s) 864, 1172, 1532, 1820, 2000
SseBI AGGCCT 2 cut(s) 1282, 2426
SspDI GGCGCC 4 cut(s) 100, 124, 175, 238
SspI AATATT 2 cut(s) 949, 2539
SspMI CTAG 2 cut(s) 1509, 2774
SstI GAGCTC 1 cut(s) 1305
StuI AGGCCT 2 cut(s) 1282, 2426
StyI CCWWGG 3 cut(s) 339, 1296, 2095
TaaI ACNGT 7 cut(s) 169, 824, 1712, 1755, 2035, 2135, 2554
TaiI ACGT 4 cut(s) 745, 1166, 1368, 1663
TaqI TCGA 8 cut(s) 161, 212, 420, 646, 865, 1123, 1654, 2324
TatI WGTACW 3 cut(s) 522, 1221, 1750
TscAI CASTG 9 cut(s) 363, 589, 1203, 1503, 1946, 2080, 2160, 2680, 2860
TseFI GTSAC 4 cut(s) 869, 988, 1357, 2107
TseI GCWGC 9 cut(s) 650, 730, 803, 1110, 1144, 2150, 2777, 2844, 2858
Tsp45I GTSAC 4 cut(s) 869, 988, 1357, 2107
TspDTI ATGAA 7 cut(s) 9, 261, 584, 1362, 2270, 2296, 2802
TspGWI ACGGA 2 cut(s) 318, 2434
TspMI CCCGGG 1 cut(s) 1392
TspRI CASTG 9 cut(s) 363, 589, 1203, 1503, 1946, 2080, 2160, 2680, 2860
Van91I CCANNNNNTGG 1 cut(s) 2123
Vha464I CTTAAG 3 cut(s) 1532, 1820, 2000
VneI GTGCAC 1 cut(s) 2728
VpaK11BI GGWCC 3 cut(s) 860, 1895, 2872
VspI ATTAAT 1 cut(s) 2007
XagI CCTNNNNNAGG 1 cut(s) 1238
XapI RAATTY 5 cut(s) 878, 1466, 1908, 2585, 2616
XcmI CCANNNNNNNNNTGG 2 cut(s) 412, 1195
XhoI CTCGAG 1 cut(s) 864
XmaI CCCGGG 1 cut(s) 1392
XmnI GAANNNNTTC 1 cut(s) 2669
XspI CTAG 2 cut(s) 1509, 2774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.