pycom03g06320

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000148
Physical Location & Seq
Reverse (-)
4948658 .. 4948948
291 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06320.1

Sequence Viewer

Length: 291 bp
ATGGCTACTAATCCTACTATTTGCGTGAAGGCCTTGGTGGACAAGGGAAGTAACGAGGTTATTTTCGTAGAGTCTGGTAATGATTTGGTTGAGACTCTCTTCAGTTACTTGATAATCCCCATGGGAACAGTTATCGAGCTTGCTCGTATACATTCAGATCCTGCGGTCATTGGCTGCATGAAAAACTTGTACGCAAGTGTTGAGAGTATTGGGGAAGAGGAAATTTTGGGTAACATATTTAAGGACTTGTACATTCATAATGTTAGGCCGCTGCGCTACTATGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

97

Amino Acids

10.72

Weight (kDa)

4.8

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 4 - 85 4.1e-22 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 148
AciI CCGC 2 cut(s) 164, 269
AclWI GGATC 1 cut(s) 152
AcsI RAATTY 1 cut(s) 222
AcuI CTGAAG 1 cut(s) 85
AfaI GTAC 2 cut(s) 191, 251
AluBI AGCT 1 cut(s) 139
AluI AGCT 1 cut(s) 139
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 152
AlwNI CAGNNNCTG 1 cut(s) 161
AoxI GGCC 2 cut(s) 30, 266
ApeKI GCWGC 2 cut(s) 174, 271
ApoI RAATTY 1 cut(s) 222
AspLEI GCGC 1 cut(s) 276
BbvI GCAGC 2 cut(s) 161, 258
BcoDI GTCTC 1 cut(s) 86
BisI GCNGC 3 cut(s) 175, 269, 272
BlsI GCNGC 3 cut(s) 176, 270, 273
BsaJI CCNNGG 2 cut(s) 33, 120
BseDI CCNNGG 2 cut(s) 33, 120
BseXI GCAGC 2 cut(s) 161, 258
BshFI GGCC 2 cut(s) 32, 268
BsmAI GTCTC 1 cut(s) 86
BsnI GGCC 2 cut(s) 32, 268
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 1 cut(s) 157
Bsp19I CCATGG 1 cut(s) 120
BspACI CCGC 2 cut(s) 164, 269
BspANI GGCC 2 cut(s) 32, 268
BspPI GGATC 1 cut(s) 152
BsrGI TGTACA 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 33, 120
BssMI GATC 1 cut(s) 157
BssNAI GTATAC 1 cut(s) 149
BssT1I CCWWGG 2 cut(s) 33, 120
Bst1107I GTATAC 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 130
Bst6I CTCTTC 2 cut(s) 104, 210
BstAUI TGTACA 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 141
BstDSI CCRYGG 1 cut(s) 120
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 1 cut(s) 160
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 1 cut(s) 157
BstV1I GCAGC 2 cut(s) 161, 258
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BstZ17I GTATAC 1 cut(s) 149
BsuRI GGCC 2 cut(s) 32, 268
BtgI CCRYGG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 161
CfoI GCGC 1 cut(s) 276
Csp6I GTAC 2 cut(s) 190, 250
CviAII CATG 2 cut(s) 121, 178
CviJI RGCY 5 cut(s) 5, 32, 139, 174, 268
CviKI_1 RGCY 5 cut(s) 5, 32, 139, 174, 268
CviQI GTAC 2 cut(s) 190, 250
DpnI GATC 1 cut(s) 159
DpnII GATC 1 cut(s) 157
Eam1104I CTCTTC 2 cut(s) 104, 210
EarI CTCTTC 2 cut(s) 104, 210
Eco130I CCWWGG 2 cut(s) 33, 120
Eco147I AGGCCT 1 cut(s) 32
Eco57I CTGAAG 1 cut(s) 85
EcoT14I CCWWGG 2 cut(s) 33, 120
ErhI CCWWGG 2 cut(s) 33, 120
FaeI CATG 2 cut(s) 124, 181
FaiI YATR 6 cut(s) 122, 149, 179, 236, 258, 282
FatI CATG 2 cut(s) 120, 177
FblI GTMKAC 1 cut(s) 148
Fnu4HI GCNGC 3 cut(s) 175, 269, 272
Fsp4HI GCNGC 3 cut(s) 175, 269, 272
GlaI GCGC 1 cut(s) 275
GluI GCNGC 3 cut(s) 175, 269, 272
HaeIII GGCC 2 cut(s) 32, 268
HhaI GCGC 1 cut(s) 276
Hin1II CATG 2 cut(s) 124, 181
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 2 cut(s) 71, 94
Hpy166II GTNNAC 2 cut(s) 40, 149
Hpy188I TCNGA 1 cut(s) 157
Hpy8I GTNNAC 2 cut(s) 40, 149
HpyAV CCTTC 1 cut(s) 22
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4V TGCA 1 cut(s) 177
Hsp92II CATG 2 cut(s) 124, 181
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 1 cut(s) 157
LpnPI CCDG 2 cut(s) 60, 174
Lsp1109I GCAGC 2 cut(s) 161, 258
MaeIII GTNAC 3 cut(s) 50, 104, 230
MalI GATC 1 cut(s) 159
MboI GATC 1 cut(s) 157
MboII GAAGA 2 cut(s) 91, 227
MflI RGATCY 1 cut(s) 157
MluCI AATT 1 cut(s) 222
MlyI GAGTC 2 cut(s) 80, 88
MnlI CCTC 2 cut(s) 49, 211
MseI TTAA 1 cut(s) 240
MspA1I CMGCKG 1 cut(s) 271
NcoI CCATGG 1 cut(s) 120
NdeII GATC 1 cut(s) 157
NlaIII CATG 2 cut(s) 124, 181
PceI AGGCCT 1 cut(s) 32
PkrI GCNGC 3 cut(s) 176, 270, 273
PleI GAGTC 2 cut(s) 79, 88
PpsI GAGTC 2 cut(s) 79, 88
PstNI CAGNNNCTG 1 cut(s) 161
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 2 cut(s) 191, 251
RsaNI GTAC 2 cut(s) 190, 250
SaqAI TTAA 1 cut(s) 240
SatI GCNGC 3 cut(s) 175, 269, 272
Sau3AI GATC 1 cut(s) 157
SchI GAGTC 2 cut(s) 80, 88
SetI ASST 2 cut(s) 60, 141
Sse9I AATT 1 cut(s) 222
SseBI AGGCCT 1 cut(s) 32
SsiI CCGC 2 cut(s) 164, 269
StuI AGGCCT 1 cut(s) 32
StyI CCWWGG 2 cut(s) 33, 120
TaaI ACNGT 1 cut(s) 130
TaqI TCGA 1 cut(s) 135
TasI AATT 1 cut(s) 222
TatI WGTACW 1 cut(s) 249
TauI GCSGC 1 cut(s) 271
Tru1I TTAA 1 cut(s) 240
Tru9I TTAA 1 cut(s) 240
TseI GCWGC 2 cut(s) 174, 271
TspDTI ATGAA 2 cut(s) 194, 245
XapI RAATTY 1 cut(s) 222
XmiI GTMKAC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.