MD11G1084000.v1.1

Protein of unknown function (DUF674)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000148
Physical Location & Seq
Reverse (-)
7095013 .. 7095783
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1084000.v1.1.491

Sequence Viewer

Length: 657 bp
ATGTATAAGGAAGAACAAAAGATTCCTATCAAGCTTATAGTGAGCAAATCTAAGACGAAGGTTTGTTATGCAGAAGCCGGGGTGGATTTCGTTAACTTAATCTTCAGTTTCTTGACTCTACCACTAGGTTTTATAGTAAAACAGATGCAAAATAACTCTATGAAAGGTAGCAATGACCGCCTTTACAGAGGTGTCCGCGATCTTGATGAGCAGTACTTGAAGTCATATCTCCACAAGAAAATGTTCCTGAGTCCGGAGCTTCCCCCTAAATATGGCTATCAGAATCATCCTTTGGGAATTGAGGAATCCTCGTATGAAGATATATCTGGCGTGCCCGCGAAAGTGATTGACTTCGTAGACCCCAAATCGCATGACAATGAAGATATTAAAGCTTGTGGATTCATGAAAGGACTAGAGATGTTCATGGTAACTGACAATCTCAATGTGAGTCCGATATCTGCAATGCTTGGCATGTCGATACTACGCGAACTGGATGTACCAGTCACTGACATTGAGGTACGAGTTGCTCATGTGAGCGCGGAAGAGGCGTTCCGTCTTTTGGTGGCTTCTTTTGTTTGCAACGCTGCTCTAACTAGTGCCTTCCTTGCGAAGCCAAAGCGAGATGGAGAATTGGGATACCTTGGTTTTCTAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF674

Protein Analysis

219

Amino Acids

24.48

Weight (kDa)

5.85

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF674 PF05056 3 - 179 6e-40 Protein of unknown function (DUF674)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G11145 AT3G09110 AT3G09120 AT3G09140 AT3G09140 AT5G01120 AT5G01130 AT5G01140 AT5G01150 AT5G37320 AT5G43240 AT5G43240 AT5G43240 AT5G43240
fragaria_vesca FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37570 FvH4_3g37580 FvH4_3g37580 FvH4_3g37590 FvH4_3g37590 FvH4_3g37590 FvH4_3g37601 FvH4_3g37610 FvH4_3g37610 FvH4_3g37640 FvH4_3g37640 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430 FvH4_6g48430
malus_domestica MD03G1079100.v1.1 MD03G1079200.v1.1 MD03G1079300.v1.1 MD03G1079500.v1.1 MD03G1079600.v1.1 MD03G1079700.v1.1 MD03G1079800.v1.1 MD11G1083300.v1.1 MD11G1083400.v1.1 MD11G1083500.v1.1 MD11G1083600.v1.1 MD11G1083700.v1.1 MD11G1083900.v1.1 MD11G1084000.v1.1 MD11G1084200.v1.1 MD11G1084300.v1.1
prunus_persica Prupe.1G077500_v2.0.a1 Prupe.6G063700_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063800_v2.0.a1 Prupe.6G063900_v2.0.a1 Prupe.6G064000_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064100_v2.0.a1 Prupe.6G064300_v2.0.a1 Prupe.6G064400_v2.0.a1
pyrus_communis pycom03g06310 pycom03g06320 pycom03g06330 pycom03g06340 pycom03g06350 pycom11g07140 pycom11g07160 pycom11g07170 pycom11g07190 pycom11g07200 pycom11g07230 pycom11g07240 pycom11g07250 pycom11g07260 pycom11g07270 pycom11g07280
rosa_chinensis RchiOBHm_Chr3g0490491 RchiOBHm_Chr5g0055481 RchiOBHm_Chr5g0067411 RchiOBHm_Chr5g0067441 RchiOBHm_Chr5g0067471 RchiOBHm_Chr5g0067491 RchiOBHm_Chr5g0067511 RchiOBHm_Chr5g0067521 RchiOBHm_Chr5g0067571 RchiOBHm_Chr5g0067581 RchiOBHm_Chr5g0067661 RchiOBHm_Chr5g0067671 RchiOBHm_Chr5g0067691 RchiOBHm_Chr5g0067721 RchiOBHm_Chr5g0067751 RchiOBHm_Chr5g0067801 RchiOBHm_Chr5g0067821 RchiOBHm_Chr5g0067831 RchiOBHm_Chr5g0067841 RchiOBHm_Chr5g0067861 RchiOBHm_Chr5g0067891 RchiOBHm_Chr5g0068351 RchiOBHm_Chr6g0278351
rosa_laevigata RLG00000013235 RLG00000022886 RLG00000035934 RLG00000035935 RLG00000035936 RLG00000035938 RLG00000035939 RLG00000035940 RLG00000035942 RLG00000035968
rosa_multiflora Rmu_sc0000230.1_g000001 Rmu_sc0000435.1_g000052 Rmu_sc0000435.1_g000053 Rmu_sc0000435.1_g000057 Rmu_sc0000435.1_g000067 Rmu_sc0000435.1_g000080 Rmu_sc0001016.1_g000006 Rmu_sc0001418.1_g000006 Rmu_sc0001418.1_g000008 Rmu_sc0002253.1_g000012 Rmu_sc0002253.1_g000013 Rmu_sc0002253.1_g000038 Rmu_sc0002253.1_g000039 Rmu_sc0002253.1_g000044 Rmu_sc0002489.1_g000055 Rmu_sc0003862.1_g000013 Rmu_sc0007131.1_g000003 Rmu_sc0007131.1_g000004 Rmu_sc0008054.1_g000001 Rmu_sc0009958.1_g000001 Rmu_sc0016063.1_g000001 Rmu_sc0022825.1_g000001
rosa_roxburghii Rroxscaffold_1G00012750 Rroxscaffold_1G00012810 Rroxscaffold_1G00012820 Rroxscaffold_1G00012860 Rroxscaffold_1G00012880 Rroxscaffold_1G00012920 Rroxscaffold_1G00012930 Rroxscaffold_1G00012940 Rroxscaffold_1G00013430 Rroxscaffold_1G00013440 Rroxscaffold_1G00013460 Rroxscaffold_1G00013510 Rroxscaffold_1G00013530 Rroxscaffold_1G00013550 Rroxscaffold_1G00013570 Rroxscaffold_1G00013580 Rroxscaffold_1G00013610 Rroxscaffold_1G00013620 Rroxscaffold_1G00013630 Rroxscaffold_1G00013680 Rroxscaffold_7G00190460
rosa_rugosa Rorug05G0192300 Rorug05G0192400 Rorug05G0192500 Rorug05G0192600 Rorug05G0192700 Rorug05G0384600 Rorug05G0385300 Rorug05G0385400 Rorug05G0385400 Rorug05G0385500 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385600 Rorug05G0385800 Rorug05G0386000 Rorug05G0389000 Rorug05G0389100 Rorug05G0397000 Rorug05G0397100
rosa_samantha Rh5BG461400 Rh5BG461600 Rh5BG461700 Rh5BG461900 Rh5BG462100 Rh6AG225700 Rh6AG225800
rosa_wichuraiana Rw0G017720 Rw3G025960 Rw5G041500 Rw5G041510 Rw5G041520 Rw5G041530 Rw5G041540 Rw5G041580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 357
AccII CGCG 4 cut(s) 198, 338, 486, 539
AccIII TCCGGA 1 cut(s) 253
AciI CCGC 4 cut(s) 178, 196, 336, 539
AcuI CTGAAG 1 cut(s) 88
AfaI GTAC 3 cut(s) 215, 498, 519
AfiI CCNNNNNNNGG 3 cut(s) 253, 272, 559
AgsI TTSAA 1 cut(s) 220
AhlI ACTAGT 1 cut(s) 593
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AloI GAACNNNNNNTCC 2 cut(s) 533, 565
AluBI AGCT 3 cut(s) 34, 259, 392
AluI AGCT 3 cut(s) 34, 259, 392
AlwNI CAGNNNCTG 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 253
ApeKI GCWGC 1 cut(s) 584
Asp700I GAANNNNTTC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 539
AsuC2I CCSGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 336
BaeI ACNNNNGTAYC 1 cut(s) 628
BbvI GCAGC 1 cut(s) 571
BccI CCATC 1 cut(s) 617
BciVI GTATCC 1 cut(s) 629
BcnI CCSGG 1 cut(s) 79
BcuI ACTAGT 1 cut(s) 593
BfaI CTAG 3 cut(s) 125, 413, 594
BfuI GTATCC 1 cut(s) 629
BisI GCNGC 1 cut(s) 585
BlsI GCNGC 1 cut(s) 586
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 135
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpuMI CCSGG 1 cut(s) 79
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 78, 640
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 272, 559
Bse1I ACTGG 2 cut(s) 495, 500
Bse3DI GCAATG 2 cut(s) 178, 468
Bse8I GATNNNNATC 1 cut(s) 26
BseAI TCCGGA 1 cut(s) 253
BseDI CCNNGG 2 cut(s) 78, 640
BseGI GGATG 2 cut(s) 286, 499
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 253, 272, 559
BseMI GCAATG 2 cut(s) 178, 468
BseMII CTCAG 1 cut(s) 239
BseNI ACTGG 2 cut(s) 495, 500
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 571
Bsh1236I CGCG 4 cut(s) 198, 338, 486, 539
BsiSI CCGG 2 cut(s) 78, 254
BslI CCNNNNNNNGG 3 cut(s) 253, 272, 559
Bsp1286I GDGCHC 1 cut(s) 336
Bsp13I TCCGGA 1 cut(s) 253
Bsp143I GATC 1 cut(s) 199
BspACI CCGC 4 cut(s) 178, 196, 336, 539
BspCNI CTCAG 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 253
BspFNI CGCG 4 cut(s) 198, 338, 486, 539
BspHI TCATGA 1 cut(s) 402
BsrDI GCAATG 2 cut(s) 178, 468
BsrI ACTGG 2 cut(s) 495, 500
BssECI CCNNGG 2 cut(s) 78, 640
BssMI GATC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 537
BstC8I GCNNGC 2 cut(s) 332, 336
BstDEI CTNAG 2 cut(s) 51, 248
BstF5I GGATG 2 cut(s) 286, 499
BstFNI CGCG 4 cut(s) 198, 338, 486, 539
BstHHI GCGC 1 cut(s) 539
BstKTI GATC 1 cut(s) 202
BstMBI GATC 1 cut(s) 199
BstMWI GCNNNNNNNGC 3 cut(s) 177, 545, 605
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 1 cut(s) 77
BstSLI GKGCMC 1 cut(s) 336
BstUI CGCG 4 cut(s) 198, 338, 486, 539
BstV1I GCAGC 1 cut(s) 571
BsuI GTATCC 1 cut(s) 629
BtsCI GGATG 2 cut(s) 286, 499
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 2 cut(s) 332, 336
CaiI CAGNNNCTG 1 cut(s) 506
CciI TCATGA 1 cut(s) 402
CfoI GCGC 1 cut(s) 539
Csp6I GTAC 3 cut(s) 214, 497, 518
CviAII CATG 5 cut(s) 371, 403, 424, 472, 530
CviJI RGCY 7 cut(s) 34, 77, 259, 276, 392, 566, 613
CviKI_1 RGCY 7 cut(s) 34, 77, 259, 276, 392, 566, 613
CviQI GTAC 3 cut(s) 214, 497, 518
DdeI CTNAG 2 cut(s) 51, 248
DpnI GATC 1 cut(s) 201
DpnII GATC 1 cut(s) 199
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 640
Eco32I GATATC 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 88
EcoRV GATATC 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 640
ErhI CCWWGG 1 cut(s) 640
FaeI CATG 5 cut(s) 374, 406, 427, 475, 533
FatI CATG 5 cut(s) 370, 402, 423, 471, 529
FauI CCCGC 1 cut(s) 343
FblI GTMKAC 1 cut(s) 357
Fnu4HI GCNGC 1 cut(s) 585
FokI GGATG 2 cut(s) 273, 506
Fsp4HI GCNGC 1 cut(s) 585
FspBI CTAG 3 cut(s) 125, 413, 594
GlaI GCGC 1 cut(s) 538
GluI GCNGC 1 cut(s) 585
HapII CCGG 2 cut(s) 78, 254
HhaI GCGC 1 cut(s) 539
Hin1II CATG 5 cut(s) 374, 406, 427, 475, 533
Hin6I GCGC 1 cut(s) 537
HinP1I GCGC 1 cut(s) 537
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 2 cut(s) 32, 390
HinfI GANTC 7 cut(s) 22, 115, 250, 283, 305, 399, 448
HpaI GTTAAC 1 cut(s) 94
HpaII CCGG 2 cut(s) 78, 254
Hpy166II GTNNAC 2 cut(s) 94, 358
Hpy188I TCNGA 2 cut(s) 282, 453
Hpy188III TCNNGA 5 cut(s) 112, 203, 247, 254, 403
Hpy8I GTNNAC 2 cut(s) 94, 358
HpyAV CCTTC 2 cut(s) 52, 610
HpyCH4V TGCA 4 cut(s) 71, 148, 461, 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 177, 545, 605
HpyF3I CTNAG 2 cut(s) 51, 248
Hsp92II CATG 5 cut(s) 374, 406, 427, 475, 533
HspAI GCGC 1 cut(s) 537
Kpn2I TCCGGA 1 cut(s) 253
KspAI GTTAAC 1 cut(s) 94
Kzo9I GATC 1 cut(s) 199
LmnI GCTCC 1 cut(s) 256
LpnPI CCDG 6 cut(s) 91, 260, 267, 312, 476, 513
Lsp1109I GCAGC 1 cut(s) 571
LweI GCATC 1 cut(s) 135
MaeI CTAG 3 cut(s) 125, 413, 594
MaeIII GTNAC 2 cut(s) 427, 502
MalI GATC 1 cut(s) 201
MboI GATC 1 cut(s) 199
MboII GAAGA 5 cut(s) 23, 94, 329, 392, 554
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 3 cut(s) 297, 629, 651
MlyI GAGTC 3 cut(s) 109, 259, 457
MnlI CCTC 5 cut(s) 182, 295, 319, 508, 538
MroI TCCGGA 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 242
MseI TTAA 4 cut(s) 93, 98, 387, 655
MslI CAYNNNNRTG 1 cut(s) 375
MspI CCGG 2 cut(s) 78, 254
MspR9I CCNGG 1 cut(s) 79
MvnI CGCG 4 cut(s) 198, 338, 486, 539
MwoI GCNNNNNNNGC 3 cut(s) 177, 545, 605
NciI CCSGG 1 cut(s) 79
NdeII GATC 1 cut(s) 199
NlaIII CATG 5 cut(s) 374, 406, 427, 475, 533
NmuCI GTSAC 1 cut(s) 502
NspI RCATGY 1 cut(s) 475
PagI TCATGA 1 cut(s) 402
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 4 cut(s) 22, 283, 305, 399
PkrI GCNGC 1 cut(s) 586
PleI GAGTC 3 cut(s) 109, 258, 456
PpsI GAGTC 3 cut(s) 109, 258, 456
PsrI GAACNNNNNNTAC 2 cut(s) 480, 512
PstNI CAGNNNCTG 1 cut(s) 506
RsaI GTAC 3 cut(s) 215, 498, 519
RsaNI GTAC 3 cut(s) 214, 497, 518
RseI CAYNNNNRTG 1 cut(s) 375
SaqAI TTAA 4 cut(s) 93, 98, 387, 655
SatI GCNGC 1 cut(s) 585
Sau3AI GATC 1 cut(s) 199
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 3 cut(s) 109, 259, 457
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 1 cut(s) 336
SetI ASST 9 cut(s) 36, 63, 130, 169, 193, 261, 394, 519, 642
SfaNI GCATC 1 cut(s) 135
SmiMI CAYNNNNRTG 1 cut(s) 375
SpeI ACTAGT 1 cut(s) 593
Sse9I AATT 3 cut(s) 297, 629, 651
SsiI CCGC 4 cut(s) 178, 196, 336, 539
SspMI CTAG 3 cut(s) 125, 413, 594
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 640
TaqI TCGA 1 cut(s) 476
TasI AATT 3 cut(s) 297, 629, 651
TatI WGTACW 1 cut(s) 213
TfiI GAWTC 4 cut(s) 22, 283, 305, 399
Tru1I TTAA 4 cut(s) 93, 98, 387, 655
Tru9I TTAA 4 cut(s) 93, 98, 387, 655
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 1 cut(s) 584
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 6 cut(s) 176, 330, 391, 393, 412, 419
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 1 cut(s) 511
XceI RCATGY 1 cut(s) 475
XmiI GTMKAC 1 cut(s) 357
XmnI GAANNNNTTC 1 cut(s) 242
XspI CTAG 3 cut(s) 125, 413, 594
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.