Protein of unknown function (DUF674)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 172 |
| AclWI | GGATC | 3 cut(s) | 615, 665, 678 |
| AcsI | RAATTY | 5 cut(s) | 190, 277, 496, 605, 662 |
| AcuI | CTGAAG | 1 cut(s) | 263 |
| AfaI | GTAC | 3 cut(s) | 258, 402, 810 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| AgsI | TTSAA | 5 cut(s) | 19, 190, 216, 302, 805 |
| AjnI | CCWGG | 2 cut(s) | 465, 535 |
| AluBI | AGCT | 6 cut(s) | 101, 124, 204, 358, 529, 546 |
| AluI | AGCT | 6 cut(s) | 101, 124, 204, 358, 529, 546 |
| Alw21I | GWGCWC | 1 cut(s) | 951 |
| AlwI | GGATC | 3 cut(s) | 615, 665, 678 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 743 |
| AoxI | GGCC | 3 cut(s) | 318, 576, 761 |
| ApeKI | GCWGC | 1 cut(s) | 98 |
| ApoI | RAATTY | 5 cut(s) | 190, 277, 496, 605, 662 |
| AspS9I | GGNCC | 1 cut(s) | 318 |
| AsuHPI | GGTGA | 1 cut(s) | 698 |
| AsuNHI | GCTAGC | 1 cut(s) | 101 |
| BamHI | GGATCC | 1 cut(s) | 670 |
| Bbv12I | GWGCWC | 1 cut(s) | 951 |
| BbvI | GCAGC | 1 cut(s) | 110 |
| BciT130I | CCWGG | 2 cut(s) | 467, 537 |
| BclI | TGATCA | 2 cut(s) | 445, 598 |
| BfaI | CTAG | 2 cut(s) | 102, 705 |
| BfuAI | ACCTGC | 1 cut(s) | 172 |
| BisI | GCNGC | 1 cut(s) | 99 |
| BlsI | GCNGC | 1 cut(s) | 100 |
| Bme1390I | CCNGG | 2 cut(s) | 467, 537 |
| BmgT120I | GGNCC | 1 cut(s) | 318 |
| BmiI | GGNNCC | 3 cut(s) | 130, 319, 672 |
| BmrFI | CCNGG | 2 cut(s) | 467, 537 |
| BmsI | GCATC | 1 cut(s) | 871 |
| BmtI | GCTAGC | 1 cut(s) | 105 |
| BsaAI | YACGTR | 1 cut(s) | 404 |
| BsaJI | CCNNGG | 4 cut(s) | 428, 536, 642, 682 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 424, 454, 566, 596 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| Bse1I | ACTGG | 1 cut(s) | 764 |
| Bse3DI | GCAATG | 1 cut(s) | 811 |
| BseBI | CCWGG | 2 cut(s) | 467, 537 |
| BseDI | CCNNGG | 4 cut(s) | 428, 536, 642, 682 |
| BseGI | GGATG | 2 cut(s) | 443, 760 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| BseMI | GCAATG | 1 cut(s) | 811 |
| BseMII | CTCAG | 1 cut(s) | 141 |
| BseNI | ACTGG | 1 cut(s) | 764 |
| BseRI | GAGGAG | 2 cut(s) | 167, 587 |
| BseXI | GCAGC | 1 cut(s) | 110 |
| BsgI | GTGCAG | 1 cut(s) | 117 |
| BshFI | GGCC | 3 cut(s) | 320, 578, 763 |
| BsiHKAI | GWGCWC | 1 cut(s) | 951 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 266, 434 |
| BsnI | GGCC | 3 cut(s) | 320, 578, 763 |
| Bsp1286I | GDGCHC | 1 cut(s) | 951 |
| Bsp143I | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| Bsp19I | CCATGG | 1 cut(s) | 682 |
| BspANI | GGCC | 3 cut(s) | 320, 578, 763 |
| BspCNI | CTCAG | 1 cut(s) | 142 |
| BspLI | GGNNCC | 3 cut(s) | 130, 319, 672 |
| BspMI | ACCTGC | 1 cut(s) | 172 |
| BspOI | GCTAGC | 1 cut(s) | 105 |
| BspPI | GGATC | 3 cut(s) | 615, 665, 678 |
| BsrDI | GCAATG | 1 cut(s) | 811 |
| BsrI | ACTGG | 1 cut(s) | 764 |
| BssECI | CCNNGG | 4 cut(s) | 428, 536, 642, 682 |
| BssMI | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| BssT1I | CCWWGG | 3 cut(s) | 428, 642, 682 |
| Bst2UI | CCWGG | 2 cut(s) | 467, 537 |
| Bst4CI | ACNGT | 2 cut(s) | 739, 786 |
| BstBAI | YACGTR | 1 cut(s) | 404 |
| BstC8I | GCNNGC | 2 cut(s) | 103, 933 |
| BstDEI | CTNAG | 1 cut(s) | 150 |
| BstDSI | CCRYGG | 1 cut(s) | 682 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 432 |
| BstF5I | GGATG | 2 cut(s) | 443, 760 |
| BstKTI | GATC | 5 cut(s) | 448, 601, 623, 673, 832 |
| BstMBI | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 928 |
| BstNI | CCWGG | 2 cut(s) | 467, 537 |
| BstNSI | RCATGY | 1 cut(s) | 845 |
| BstSCI | CCNGG | 2 cut(s) | 465, 535 |
| BstV1I | GCAGC | 1 cut(s) | 110 |
| BstX2I | RGATCY | 2 cut(s) | 620, 670 |
| BstYI | RGATCY | 2 cut(s) | 620, 670 |
| BsuRI | GGCC | 3 cut(s) | 320, 578, 763 |
| BtgI | CCRYGG | 1 cut(s) | 682 |
| BtsCI | GGATG | 2 cut(s) | 443, 760 |
| BtsIMutI | CAGTG | 4 cut(s) | 147, 228, 816, 949 |
| BveI | ACCTGC | 1 cut(s) | 172 |
| Cac8I | GCNNGC | 2 cut(s) | 103, 933 |
| CaiI | CAGNNNCTG | 1 cut(s) | 743 |
| Cfr13I | GGNCC | 1 cut(s) | 318 |
| CseI | GACGC | 1 cut(s) | 71 |
| Csp6I | GTAC | 3 cut(s) | 257, 401, 809 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 363, 398 |
| CviAII | CATG | 4 cut(s) | 516, 580, 683, 842 |
| CviQI | GTAC | 3 cut(s) | 257, 401, 809 |
| DdeI | CTNAG | 1 cut(s) | 150 |
| DpnI | GATC | 5 cut(s) | 447, 600, 622, 672, 831 |
| DpnII | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| Eco130I | CCWWGG | 3 cut(s) | 428, 642, 682 |
| Eco57I | CTGAAG | 1 cut(s) | 263 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 432 |
| EcoO109I | RGGNCCY | 1 cut(s) | 318 |
| EcoRII | CCWGG | 2 cut(s) | 465, 535 |
| EcoT14I | CCWWGG | 3 cut(s) | 428, 642, 682 |
| ErhI | CCWWGG | 3 cut(s) | 428, 642, 682 |
| FaeI | CATG | 4 cut(s) | 519, 583, 686, 845 |
| FatI | CATG | 4 cut(s) | 515, 579, 682, 841 |
| FbaI | TGATCA | 2 cut(s) | 445, 598 |
| Fnu4HI | GCNGC | 1 cut(s) | 99 |
| FokI | GGATG | 2 cut(s) | 450, 767 |
| Fsp4HI | GCNGC | 1 cut(s) | 99 |
| FspBI | CTAG | 2 cut(s) | 102, 705 |
| GluI | GCNGC | 1 cut(s) | 99 |
| HaeIII | GGCC | 3 cut(s) | 320, 578, 763 |
| HgaI | GACGC | 1 cut(s) | 71 |
| Hin1II | CATG | 4 cut(s) | 519, 583, 686, 845 |
| HincII | GTYRAC | 1 cut(s) | 940 |
| HindII | GTYRAC | 1 cut(s) | 940 |
| HindIII | AAGCTT | 2 cut(s) | 122, 527 |
| HinfI | GANTC | 6 cut(s) | 82, 174, 302, 371, 422, 711 |
| HphI | GGTGA | 1 cut(s) | 698 |
| Hpy166II | GTNNAC | 2 cut(s) | 117, 940 |
| Hpy188I | TCNGA | 2 cut(s) | 598, 889 |
| Hpy8I | GTNNAC | 2 cut(s) | 117, 940 |
| HpyAV | CCTTC | 3 cut(s) | 13, 642, 799 |
| HpyCH4III | ACNGT | 2 cut(s) | 739, 786 |
| HpyCH4IV | ACGT | 2 cut(s) | 403, 471 |
| HpyCH4V | TGCA | 7 cut(s) | 98, 395, 456, 519, 816, 841, 862 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 928 |
| HpyF3I | CTNAG | 1 cut(s) | 150 |
| HpySE526I | ACGT | 2 cut(s) | 403, 471 |
| Hsp92II | CATG | 4 cut(s) | 519, 583, 686, 845 |
| Ksp22I | TGATCA | 2 cut(s) | 445, 598 |
| Kzo9I | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| LmnI | GCTCC | 2 cut(s) | 128, 928 |
| LpnPI | CCDG | 9 cut(s) | 167, 267, 404, 452, 479, 522, 549, 777, 917 |
| Lsp1109I | GCAGC | 1 cut(s) | 110 |
| LweI | GCATC | 1 cut(s) | 871 |
| MaeI | CTAG | 2 cut(s) | 102, 705 |
| MaeII | ACGT | 2 cut(s) | 403, 471 |
| MaeIII | GTNAC | 1 cut(s) | 739 |
| MalI | GATC | 5 cut(s) | 447, 600, 622, 672, 831 |
| MboI | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| MboII | GAAGA | 1 cut(s) | 46 |
| MflI | RGATCY | 2 cut(s) | 620, 670 |
| MhlI | GDGCHC | 1 cut(s) | 951 |
| MlyI | GAGTC | 1 cut(s) | 183 |
| MnlI | CCTC | 8 cut(s) | 145, 325, 400, 428, 565, 568, 640, 847 |
| MseI | TTAA | 5 cut(s) | 92, 489, 660, 716, 795 |
| MspR9I | CCNGG | 2 cut(s) | 467, 537 |
| MvaI | CCWGG | 2 cut(s) | 467, 537 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 928 |
| NcoI | CCATGG | 1 cut(s) | 682 |
| NdeII | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| NheI | GCTAGC | 1 cut(s) | 101 |
| NlaIII | CATG | 4 cut(s) | 519, 583, 686, 845 |
| NlaIV | GGNNCC | 3 cut(s) | 130, 319, 672 |
| NspI | RCATGY | 1 cut(s) | 845 |
| PfeI | GAWTC | 5 cut(s) | 82, 302, 371, 422, 711 |
| PfoI | TCCNGGA | 1 cut(s) | 465 |
| PkrI | GCNGC | 1 cut(s) | 100 |
| PleI | GAGTC | 1 cut(s) | 182 |
| PpsI | GAGTC | 1 cut(s) | 182 |
| Ppu21I | YACGTR | 1 cut(s) | 404 |
| Psp6I | CCWGG | 2 cut(s) | 465, 535 |
| PspGI | CCWGG | 2 cut(s) | 465, 535 |
| PspN4I | GGNNCC | 3 cut(s) | 130, 319, 672 |
| PspPI | GGNCC | 1 cut(s) | 318 |
| PstNI | CAGNNNCTG | 1 cut(s) | 743 |
| PsuI | RGATCY | 2 cut(s) | 620, 670 |
| RsaI | GTAC | 3 cut(s) | 258, 402, 810 |
| RsaNI | GTAC | 3 cut(s) | 257, 401, 809 |
| SaqAI | TTAA | 5 cut(s) | 92, 489, 660, 716, 795 |
| SatI | GCNGC | 1 cut(s) | 99 |
| Sau3AI | GATC | 5 cut(s) | 445, 598, 620, 670, 829 |
| Sau96I | GGNCC | 1 cut(s) | 318 |
| SchI | GAGTC | 1 cut(s) | 183 |
| ScrFI | CCNGG | 2 cut(s) | 467, 537 |
| SduI | GDGCHC | 1 cut(s) | 951 |
| SfaNI | GCATC | 1 cut(s) | 871 |
| SspI | AATATT | 1 cut(s) | 732 |
| SspMI | CTAG | 2 cut(s) | 102, 705 |
| StyD4I | CCNGG | 2 cut(s) | 465, 535 |
| StyI | CCWWGG | 3 cut(s) | 428, 642, 682 |
| TaaI | ACNGT | 2 cut(s) | 739, 786 |
| TaiI | ACGT | 2 cut(s) | 406, 474 |
| TaqI | TCGA | 2 cut(s) | 177, 781 |
| TfiI | GAWTC | 5 cut(s) | 82, 302, 371, 422, 711 |
| Tru1I | TTAA | 5 cut(s) | 92, 489, 660, 716, 795 |
| Tru9I | TTAA | 5 cut(s) | 92, 489, 660, 716, 795 |
| TscAI | CASTG | 3 cut(s) | 154, 235, 823 |
| TseI | GCWGC | 1 cut(s) | 98 |
| TspDTI | ATGAA | 1 cut(s) | 46 |
| TspGWI | ACGGA | 1 cut(s) | 931 |
| TspRI | CASTG | 3 cut(s) | 154, 235, 823 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 432 |
| XapI | RAATTY | 5 cut(s) | 190, 277, 496, 605, 662 |
| XceI | RCATGY | 1 cut(s) | 845 |
| XspI | CTAG | 2 cut(s) | 102, 705 |