AT3G14250

E3 ubiquitin-protein ligase RNF217

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000271
Physical Location & Seq
Reverse (-)
4745926 .. 4746958
1033 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G14250.1

Sequence Viewer

Length: 912 bp
ATGGGTTCATCATCATCGTCGTCAAGCACAAAGGTGGACACCGATATGTTGGTAGACGAAACCTACCTCTCTGCTTTATTCGATTACGATGAAATTTTCCCAATCGCCGACGAAAGCTATGCTACGGAGATCCATCTCCAAGAAGCTATGTTTTCTTCTTTTGGCGCTTCTACTGTTGGTGTTAATCATCATCCTCAAGTCCATAGGAATCTAGTCACGCTGGTTAAGCAGGAACCGGAGATTAAAGCCGAGAATGAACCCATGGAACCATCAAGAAGGTTATGTATGATCTGTATGGACGAAAAACCTTCCTCAGACATCTTCCGAGGAACCACTAACTGCACTCACGCCTATTGCACCGACTGTACTGTCCGTTACGTGGCAACTAAGATAAAAGAAAACGCATCGAGGATCAAATGCCCTGACGTGGAGTGCACGCGACTGATAGAGCCTTACACGTGTCGAGATCTAATCCCTAAGGATGTATTCGACCGTTGGGAGAAAATCTTGTGCGAGTCTTTGATCTCCTCGTGGGACAAATTCTATTGTCCGTTCAAAGATTGTTCGGCGATGATGGTGAACAATGAAAATGGAGATGCTAACGTGACGCAGACGGAGTGTCGTTCTTGTCATAGACTGTTCTGCGTGCAGTGTAAGGTGACATGGCATGCGGGGATTGGGTGTGATGAGTTTCAAAGATTTGGGAATACGAAGAAGAAGAGTAGTGATGAAGACGATGCTTTGTTGATTCAGATGGCAAAAAATAAGCAGTGGAGAAGGTGTCCTAGTTGCAAATTCTATGTTGATAAAGTCGAGGGTTGTCAACATATAAAATGCAGGTGTGGATATCAGTTTTGCTATGGTTGTGGATCTGTGTGGAGTTCTTCTCACGCATGCCAAATTCGTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-C3HC4 zf-RING_UBOX

Protein Analysis

303

Amino Acids

34.61

Weight (kDa)

5.88

Isoelectric Point (pI)

54.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 165 - 228 7.4e-11 IBR domain, a half RING-finger domain
IBR PF01485 252 - 299 3e-08 IBR domain, a half RING-finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14250
fragaria_vesca FvH4_1g09490 FvH4_1g09500 FvH4_1g09500 FvH4_1g09510 FvH4_3g00870 FvH4_3g03010 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g35700 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220
malus_domestica MD02G1102400.v1.1 MD02G1103700.v1.1 MD03G1111900.v1.1 MD09G1173800.v1.1 MD09G1230900.v1.1 MD09G1231400.v1.1 MD15G1224200.v1.1
prunus_persica Prupe.3G031900_v2.0.a1 Prupe.3G107200_v2.0.a1 Prupe.7G191200_v2.0.a1 Prupe.7G191800_v2.0.a1 Prupe.8G007700_v2.0.a1
pyrus_communis pycom02g08010 pycom02g08140 pycom09g14980 pycom09g15030 pycom15g19900
rosa_chinensis RchiOBHm_Chr2g0096041 RchiOBHm_Chr2g0096051 RchiOBHm_Chr2g0096061 RchiOBHm_Chr2g0096071 RchiOBHm_Chr2g0096081 RchiOBHm_Chr2g0096221 RchiOBHm_Chr2g0131511 RchiOBHm_Chr2g0147691 RchiOBHm_Chr5g0001281 RchiOBHm_Chr5g0001301
rosa_laevigata RLG00000016619 RLG00000016620 RLG00000016621 RLG00000016622 RLG00000019200 RLG00000020271 RLG00000025410 RLG00000030919 RLG00000031102 RLG00000031117 RLG00000031119 RLG00000031120
rosa_multiflora Rmu_co8160592.1_g000001 Rmu_sc0001706.1_g000008 Rmu_sc0002494.1_g000005 Rmu_sc0004189.1_g000040 Rmu_sc0005612.1_g000008 Rmu_sc0005612.1_g000011 Rmu_sc0005612.1_g000012 Rmu_sc0006926.1_g000015 Rmu_sc0013257.1_g000002 Rmu_sc0034363.1_g000001 Rmu_sc0034363.1_g000002
rosa_roxburghii Rroxscaffold_1G00072690 Rroxscaffold_1G00075020 Rroxscaffold_2G00145780 Rroxscaffold_2G00145790 Rroxscaffold_2G00145800 Rroxscaffold_2G00145810 Rroxscaffold_2G00145820 Rroxscaffold_2G00145830 Rroxscaffold_4G00300930 Rroxscaffold_6G00424750
rosa_rugosa Rorug02G0056000 Rorug02G0056100 Rorug02G0056200 Rorug02G0056300 Rorug02G0056500 Rorug02G0056700 Rorug02G0298000.1 Rorug02G0400900 Rorug02G0401000 Rorug02G0401100 Rorug04G0389500 Rorug04G0389600 Rorug04G0389700 Rorug04G0402300
rosa_samantha Rh2AG103800 Rh2AG458300 Rh2BG105400 Rh2BG105500 Rh2BG105600 Rh2BG105700 Rh2BG105900 Rh2BG357600 Rh2BG471000 Rh2DG106000 Rh2DG106100 Rh2DG106200 Rh2DG106300 Rh2DG106500 Rh2DG375400 Rh2DG480300 Rh5AG009500 Rh5AG009600 Rh5AG010000 Rh5AG028300 Rh5BG012400 Rh5BG012500 Rh5BG012700 Rh5BG028500
rosa_wichuraiana Rw2G007950 Rw2G007960 Rw2G007970 Rw2G028490 Rw5G000980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 828
AasI GACNNNNNNGTC 1 cut(s) 368
Acc36I ACCTGC 1 cut(s) 828
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 439
AciI CCGC 1 cut(s) 671
AclWI GGATC 3 cut(s) 124, 419, 877
AcsI RAATTY 4 cut(s) 93, 539, 794, 900
AcvI CACGTG 1 cut(s) 459
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 2 cut(s) 379, 427
AflIII ACRYGT 2 cut(s) 456, 458
AgsI TTSAA 2 cut(s) 556, 695
AhdI GACNNNNNGTC 1 cut(s) 618
AjiI CACGTC 1 cut(s) 427
AleI CACNNNNGTG 1 cut(s) 32
AluBI AGCT 2 cut(s) 117, 146
AluI AGCT 2 cut(s) 117, 146
Alw21I GWGCWC 1 cut(s) 437
Alw44I GTGCAC 1 cut(s) 433
AlwI GGATC 3 cut(s) 124, 419, 877
ApaLI GTGCAC 1 cut(s) 433
ApoI RAATTY 4 cut(s) 93, 539, 794, 900
AspLEI GCGC 1 cut(s) 167
AsuHPI GGTGA 2 cut(s) 589, 670
AxyI CCTNAGG 1 cut(s) 477
BaeGI GKGCMC 1 cut(s) 437
BauI CACGAG 1 cut(s) 529
BbrPI CACGTG 1 cut(s) 459
BbsI GAAGAC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 437
BccI CCATC 4 cut(s) 141, 277, 568, 748
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BfaI CTAG 2 cut(s) 212, 786
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 1 cut(s) 828
BglII AGATCT 1 cut(s) 466
BmeRI GACNNNNNGTC 1 cut(s) 618
BmgBI CACGTC 1 cut(s) 427
BmiI GGNNCC 3 cut(s) 234, 267, 331
BmsI GCATC 3 cut(s) 413, 586, 727
BpiI GAAGAC 1 cut(s) 738
BplI GAGNNNNNCTC 2 cut(s) 871, 903
BpuEI CTTGAG 1 cut(s) 180
BsaAI YACGTR 2 cut(s) 379, 459
BsaJI CCNNGG 2 cut(s) 261, 325
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 427
Bse21I CCTNAGG 1 cut(s) 477
BseDI CCNNGG 2 cut(s) 261, 325
BseGI GGATG 2 cut(s) 190, 487
BseLI CCNNNNNNNGG 2 cut(s) 379, 427
BseMII CTCAG 1 cut(s) 327
BseRI GAGGAG 1 cut(s) 517
BseSI GKGCMC 1 cut(s) 437
BsgI GTGCAG 2 cut(s) 325, 668
Bsh1236I CGCG 1 cut(s) 439
Bsh1285I CGRYCG 1 cut(s) 493
BsiEI CGRYCG 1 cut(s) 493
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 236
BslFI GGGAC 1 cut(s) 548
BslI CCNNNNNNNGG 2 cut(s) 379, 427
BsmFI GGGAC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 6 cut(s) 129, 288, 411, 466, 522, 869
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 1 cut(s) 671
BspCNI CTCAG 1 cut(s) 326
BspFNI CGCG 1 cut(s) 439
BspLI GGNNCC 3 cut(s) 234, 267, 331
BspMI ACCTGC 1 cut(s) 828
BspPI GGATC 3 cut(s) 124, 419, 877
BssECI CCNNGG 2 cut(s) 261, 325
BssMI GATC 6 cut(s) 129, 288, 411, 466, 522, 869
BssSI CACGAG 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 261
Bst2BI CACGAG 1 cut(s) 529
Bst4CI ACNGT 5 cut(s) 175, 365, 370, 494, 639
Bst6I CTCTTC 1 cut(s) 713
BstBAI YACGTR 2 cut(s) 379, 459
BstC8I GCNNGC 4 cut(s) 437, 647, 669, 895
BstDEI CTNAG 3 cut(s) 313, 387, 477
BstDSI CCRYGG 1 cut(s) 261
BstF5I GGATG 2 cut(s) 190, 487
BstFNI CGCG 1 cut(s) 439
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 6 cut(s) 132, 291, 414, 469, 525, 872
BstMBI GATC 6 cut(s) 129, 288, 411, 466, 522, 869
BstMCI CGRYCG 1 cut(s) 493
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstNSI RCATGY 2 cut(s) 671, 897
BstSLI GKGCMC 1 cut(s) 437
BstUI CGCG 1 cut(s) 439
BstV2I GAAGAC 1 cut(s) 738
BstX2I RGATCY 3 cut(s) 129, 466, 869
BstYI RGATCY 3 cut(s) 129, 466, 869
Bsu36I CCTNAGG 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 584
BtrI CACGTC 1 cut(s) 427
BtsCI GGATG 2 cut(s) 190, 487
BtsI GCAGTG 2 cut(s) 656, 776
BtsIMutI CAGTG 2 cut(s) 656, 776
BveI ACCTGC 1 cut(s) 828
Cac8I GCNNGC 4 cut(s) 437, 647, 669, 895
CfoI GCGC 1 cut(s) 167
CseI GACGC 1 cut(s) 616
Csp6I GTAC 1 cut(s) 366
CviAII CATG 4 cut(s) 262, 663, 668, 894
CviJI RGCY 4 cut(s) 117, 146, 248, 451
CviKI_1 RGCY 4 cut(s) 117, 146, 248, 451
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 3 cut(s) 313, 387, 477
DpnI GATC 6 cut(s) 131, 290, 413, 468, 524, 871
DpnII GATC 6 cut(s) 129, 288, 411, 466, 522, 869
DrdI GACNNNNNNGTC 1 cut(s) 368
DriI GACNNNNNGTC 1 cut(s) 618
DseDI GACNNNNNNGTC 1 cut(s) 368
Eam1104I CTCTTC 1 cut(s) 713
Eam1105I GACNNNNNGTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 713
Eco130I CCWWGG 1 cut(s) 261
Eco32I GATATC 1 cut(s) 848
Eco72I CACGTG 1 cut(s) 459
Eco81I CCTNAGG 1 cut(s) 477
EcoRV GATATC 1 cut(s) 848
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 4 cut(s) 265, 666, 671, 897
FaqI GGGAC 1 cut(s) 548
FatI CATG 4 cut(s) 261, 662, 667, 893
FauI CCCGC 1 cut(s) 664
FblI GTMKAC 1 cut(s) 54
FokI GGATG 2 cut(s) 177, 494
FspBI CTAG 2 cut(s) 212, 786
GlaI GCGC 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 168
HapII CCGG 1 cut(s) 236
HgaI GACGC 1 cut(s) 616
HhaI GCGC 1 cut(s) 167
Hin1II CATG 4 cut(s) 265, 666, 671, 897
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HincII GTYRAC 1 cut(s) 824
HindII GTYRAC 1 cut(s) 824
HinfI GANTC 3 cut(s) 208, 515, 748
HpaII CCGG 1 cut(s) 236
HphI GGTGA 2 cut(s) 589, 670
Hpy166II GTNNAC 5 cut(s) 37, 55, 435, 580, 824
Hpy188I TCNGA 3 cut(s) 316, 326, 753
Hpy188III TCNNGA 2 cut(s) 273, 464
Hpy8I GTNNAC 5 cut(s) 37, 55, 435, 580, 824
Hpy99I CGWCG 2 cut(s) 22, 113
HpyAV CCTTC 3 cut(s) 270, 318, 771
HpyCH4III ACNGT 5 cut(s) 175, 365, 370, 494, 639
HpyCH4IV ACGT 4 cut(s) 378, 426, 458, 603
HpyCH4V TGCA 6 cut(s) 342, 357, 435, 649, 792, 837
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 3 cut(s) 313, 387, 477
HpySE526I ACGT 4 cut(s) 378, 426, 458, 603
Hsp92II CATG 4 cut(s) 265, 666, 671, 897
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 6 cut(s) 129, 288, 411, 466, 522, 869
LpnPI CCDG 5 cut(s) 206, 215, 249, 435, 823
LweI GCATC 3 cut(s) 413, 586, 727
MaeI CTAG 2 cut(s) 212, 786
MaeII ACGT 4 cut(s) 378, 426, 458, 603
MaeIII GTNAC 4 cut(s) 214, 374, 604, 658
MalI GATC 6 cut(s) 131, 290, 413, 468, 524, 871
MboI GATC 6 cut(s) 129, 288, 411, 466, 522, 869
MboII GAAGA 7 cut(s) 147, 313, 724, 727, 730, 743, 876
MflI RGATCY 3 cut(s) 129, 466, 869
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 4 cut(s) 93, 539, 794, 900
MlyI GAGTC 1 cut(s) 524
MnlI CCTC 7 cut(s) 77, 204, 320, 322, 402, 538, 808
MseI TTAA 3 cut(s) 183, 225, 243
MslI CAYNNNNRTG 2 cut(s) 32, 44
MspI CCGG 1 cut(s) 236
MvnI CGCG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 226
NcoI CCATGG 1 cut(s) 261
NdeII GATC 6 cut(s) 129, 288, 411, 466, 522, 869
NlaIII CATG 4 cut(s) 265, 666, 671, 897
NlaIV GGNNCC 3 cut(s) 234, 267, 331
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 3 cut(s) 214, 604, 658
NspI RCATGY 2 cut(s) 671, 897
OliI CACNNNNGTG 1 cut(s) 32
PaeI GCATGC 2 cut(s) 671, 897
PaqCI CACCTGC 1 cut(s) 828
PfeI GAWTC 2 cut(s) 208, 748
PleI GAGTC 1 cut(s) 523
PmaCI CACGTG 1 cut(s) 459
PmlI CACGTG 1 cut(s) 459
PpsI GAGTC 1 cut(s) 523
Ppu21I YACGTR 2 cut(s) 379, 459
PspCI CACGTG 1 cut(s) 459
PspN4I GGNNCC 3 cut(s) 234, 267, 331
PsuI RGATCY 3 cut(s) 129, 466, 869
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
RseI CAYNNNNRTG 2 cut(s) 32, 44
SaqAI TTAA 3 cut(s) 183, 225, 243
Sau3AI GATC 6 cut(s) 129, 288, 411, 466, 522, 869
SchI GAGTC 1 cut(s) 524
SduI GDGCHC 1 cut(s) 437
SfaNI GCATC 3 cut(s) 413, 586, 727
SmiMI CAYNNNNRTG 2 cut(s) 32, 44
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
SphI GCATGC 2 cut(s) 671, 897
Sse9I AATT 4 cut(s) 93, 539, 794, 900
SsiI CCGC 1 cut(s) 671
SspMI CTAG 2 cut(s) 212, 786
StyI CCWWGG 1 cut(s) 261
TaaI ACNGT 5 cut(s) 175, 365, 370, 494, 639
TaiI ACGT 4 cut(s) 381, 429, 461, 606
TaqI TCGA 5 cut(s) 81, 407, 463, 489, 813
TasI AATT 4 cut(s) 93, 539, 794, 900
TatI WGTACW 1 cut(s) 365
TfiI GAWTC 2 cut(s) 208, 748
Tru1I TTAA 3 cut(s) 183, 225, 243
Tru9I TTAA 3 cut(s) 183, 225, 243
TscAI CASTG 2 cut(s) 656, 776
TseFI GTSAC 3 cut(s) 214, 604, 658
Tsp45I GTSAC 3 cut(s) 214, 604, 658
TspDTI ATGAA 4 cut(s) 105, 270, 600, 744
TspGWI ACGGA 4 cut(s) 140, 362, 540, 629
TspRI CASTG 2 cut(s) 656, 776
VneI GTGCAC 1 cut(s) 433
XapI RAATTY 4 cut(s) 93, 539, 794, 900
XceI RCATGY 2 cut(s) 671, 897
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 2 cut(s) 212, 786
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.