MD09G1173800.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000271
Physical Location & Seq
Forward (+)
14741978 .. 14742671
694 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1173800.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGAGGGAACATCATCACCAAGCTCTGATAATAACACTCTTCTTTCAGTTGATGAGTTCTACTTTTCAGCTCTCCATGATGATGATGAAGTATTTCCCATCTCAGATGAGAAATATGCACAAGAGTTGCAGCTTCAAGAGGCTCTAATGTCCTCTGCAATTGCTTCATTAAGCCCCCATATTACTACTCAGCCCCCGAAAATCGGGGAAGGTGGTGCTGAACCCCTAATGAAAATGTTGAAGGGGAAGGAAAAAGAAACTGGACAATCCTCTGAAGGTTTTTGCGGGATCTGCTTGGACGTAAAATCGACCGGAGAAATGTTCAGAAGCAGTGGCTGCAGCCACACATTTTGCTGTGATTGCATTGGGAGATATGTTGGGACAAAAATTCAGGAAAATGTTTCAGTGGTGAAGTGTCCTGACGTGAAGTGCAAAGGTGAGTTGGAGCCACAGTCATGCAAGTCAATTATTCCTAAGGAAGTGTTTGATAGGTGGCAAAATGCACTTTGTGAATCAATGTTTCTGGGGTCTCAGAAGGTTTACTGCCCTTTTAAGGACTGCTCCGCCATGTTGGTGGATGATGGAGAAGAGGTGGTGACTGTCACCTGGTGCCCGCATTGCTGGAGAATGTTCTGTGCTCAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RING_UBOX

Protein Analysis

216

Amino Acids

23.76

Weight (kDa)

4.71

Isoelectric Point (pI)

47.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14250
fragaria_vesca FvH4_1g09490 FvH4_1g09500 FvH4_1g09500 FvH4_1g09510 FvH4_3g00870 FvH4_3g03010 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g35700 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220
malus_domestica MD02G1102400.v1.1 MD02G1103700.v1.1 MD03G1111900.v1.1 MD09G1173800.v1.1 MD09G1230900.v1.1 MD09G1231400.v1.1 MD15G1224200.v1.1
prunus_persica Prupe.3G031900_v2.0.a1 Prupe.3G107200_v2.0.a1 Prupe.7G191200_v2.0.a1 Prupe.7G191800_v2.0.a1 Prupe.8G007700_v2.0.a1
pyrus_communis pycom02g08010 pycom02g08140 pycom09g14980 pycom09g15030 pycom15g19900
rosa_chinensis RchiOBHm_Chr2g0096041 RchiOBHm_Chr2g0096051 RchiOBHm_Chr2g0096061 RchiOBHm_Chr2g0096071 RchiOBHm_Chr2g0096081 RchiOBHm_Chr2g0096221 RchiOBHm_Chr2g0131511 RchiOBHm_Chr2g0147691 RchiOBHm_Chr5g0001281 RchiOBHm_Chr5g0001301
rosa_laevigata RLG00000016619 RLG00000016620 RLG00000016621 RLG00000016622 RLG00000019200 RLG00000020271 RLG00000025410 RLG00000030919 RLG00000031102 RLG00000031117 RLG00000031119 RLG00000031120
rosa_multiflora Rmu_co8160592.1_g000001 Rmu_sc0001706.1_g000008 Rmu_sc0002494.1_g000005 Rmu_sc0004189.1_g000040 Rmu_sc0005612.1_g000008 Rmu_sc0005612.1_g000011 Rmu_sc0005612.1_g000012 Rmu_sc0006926.1_g000015 Rmu_sc0013257.1_g000002 Rmu_sc0034363.1_g000001 Rmu_sc0034363.1_g000002
rosa_roxburghii Rroxscaffold_1G00072690 Rroxscaffold_1G00075020 Rroxscaffold_2G00145780 Rroxscaffold_2G00145790 Rroxscaffold_2G00145800 Rroxscaffold_2G00145810 Rroxscaffold_2G00145820 Rroxscaffold_2G00145830 Rroxscaffold_4G00300930 Rroxscaffold_6G00424750
rosa_rugosa Rorug02G0056000 Rorug02G0056100 Rorug02G0056200 Rorug02G0056300 Rorug02G0056500 Rorug02G0056700 Rorug02G0298000.1 Rorug02G0400900 Rorug02G0401000 Rorug02G0401100 Rorug04G0389500 Rorug04G0389600 Rorug04G0389700 Rorug04G0402300
rosa_samantha Rh2AG103800 Rh2AG458300 Rh2BG105400 Rh2BG105500 Rh2BG105600 Rh2BG105700 Rh2BG105900 Rh2BG357600 Rh2BG471000 Rh2DG106000 Rh2DG106100 Rh2DG106200 Rh2DG106300 Rh2DG106500 Rh2DG375400 Rh2DG480300 Rh5AG009500 Rh5AG009600 Rh5AG010000 Rh5AG028300 Rh5BG012400 Rh5BG012500 Rh5BG012700 Rh5BG028500
rosa_wichuraiana Rw2G007950 Rw2G007960 Rw2G007970 Rw2G028490 Rw5G000980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 609
AciI CCGC 3 cut(s) 285, 564, 614
AclWI GGATC 1 cut(s) 296
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 294
AdeI CACNNNGTG 2 cut(s) 509, 609
AfiI CCNNNNNNNGG 2 cut(s) 203, 553
AgsI TTSAA 2 cut(s) 137, 241
AjiI CACGTC 1 cut(s) 424
AjnI CCWGG 1 cut(s) 605
AluBI AGCT 3 cut(s) 24, 71, 133
AluI AGCT 3 cut(s) 24, 71, 133
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 534
AlwI GGATC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 336
ApeKI GCWGC 3 cut(s) 130, 336, 339
ApoI RAATTY 1 cut(s) 387
Asp700I GAANNNNTTC 1 cut(s) 93
AsuHPI GGTGA 5 cut(s) 9, 421, 449, 595, 607
AxyI CCTNAGG 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 614
BanI GGYRCC 1 cut(s) 609
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 3 cut(s) 142, 323, 351
BccI CCATC 2 cut(s) 107, 575
BciT130I CCWGG 1 cut(s) 607
BcoDI GTCTC 1 cut(s) 534
BfmI CTRYAG 1 cut(s) 337
BisI GCNGC 3 cut(s) 131, 337, 340
BlsI GCNGC 3 cut(s) 132, 338, 341
Bme1390I CCNGG 1 cut(s) 607
BmgBI CACGTC 1 cut(s) 424
BmiI GGNNCC 2 cut(s) 447, 611
BmrFI CCNGG 1 cut(s) 607
BpmI CTGGAG 1 cut(s) 643
BsaI GGTCTC 1 cut(s) 534
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 203, 553
Bse1I ACTGG 1 cut(s) 265
Bse21I CCTNAGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 616
BseBI CCWGG 1 cut(s) 607
BseGI GGATG 1 cut(s) 583
BseLI CCNNNNNNNGG 2 cut(s) 203, 553
BseMI GCAATG 1 cut(s) 616
BseMII CTCAG 3 cut(s) 117, 203, 545
BseNI ACTGG 1 cut(s) 265
BseSI GKGCMC 1 cut(s) 614
BseXI GCAGC 3 cut(s) 142, 323, 351
Bsh1285I CGRYCG 1 cut(s) 312
BshNI GGYRCC 1 cut(s) 609
BsiEI CGRYCG 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 2 cut(s) 203, 553
BsmAI GTCTC 1 cut(s) 534
BsmFI GGGAC 1 cut(s) 394
Bso31I GGTCTC 1 cut(s) 534
Bsp1286I GDGCHC 2 cut(s) 614, 640
Bsp143I GATC 1 cut(s) 288
BspACI CCGC 3 cut(s) 285, 564, 614
BspCNI CTCAG 3 cut(s) 116, 202, 544
BspLI GGNNCC 2 cut(s) 447, 611
BspMAI CTGCAG 1 cut(s) 341
BspPI GGATC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 609
BspTNI GGTCTC 1 cut(s) 534
BsrDI GCAATG 1 cut(s) 616
BsrI ACTGG 1 cut(s) 265
BssMI GATC 1 cut(s) 288
Bst2UI CCWGG 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 453, 601
Bst6I CTCTTC 2 cut(s) 45, 582
BstAPI GCANNNNNTGC 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 614
BstDEI CTNAG 4 cut(s) 103, 189, 474, 531
BstF5I GGATG 1 cut(s) 583
BstKTI GATC 1 cut(s) 291
BstMAI GTCTC 1 cut(s) 534
BstMBI GATC 1 cut(s) 288
BstMCI CGRYCG 1 cut(s) 312
BstMWI GCNNNNNNNGC 4 cut(s) 291, 336, 360, 618
BstNI CCWGG 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 605
BstSFI CTRYAG 1 cut(s) 337
BstSLI GKGCMC 1 cut(s) 614
BstV1I GCAGC 3 cut(s) 142, 323, 351
BstX2I RGATCY 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 574
BstYI RGATCY 1 cut(s) 288
Bsu36I CCTNAGG 1 cut(s) 474
BtrI CACGTC 1 cut(s) 424
BtsCI GGATG 1 cut(s) 583
BtsI GCAGTG 1 cut(s) 337
BtsIMutI CAGTG 2 cut(s) 337, 411
Cac8I GCNNGC 1 cut(s) 614
CaiI CAGNNNCTG 1 cut(s) 336
CsiI ACCWGGT 1 cut(s) 605
CspCI CAANNNNNGTGG 2 cut(s) 332, 367
CviAII CATG 3 cut(s) 77, 456, 568
CviJI RGCY 9 cut(s) 24, 71, 133, 143, 174, 193, 336, 342, 448
CviKI_1 RGCY 9 cut(s) 24, 71, 133, 143, 174, 193, 336, 342, 448
DdeI CTNAG 4 cut(s) 103, 189, 474, 531
DpnI GATC 1 cut(s) 290
DpnII GATC 1 cut(s) 288
DraIII CACNNNGTG 2 cut(s) 509, 609
Eam1104I CTCTTC 2 cut(s) 45, 582
EarI CTCTTC 2 cut(s) 45, 582
EciI GGCGGA 1 cut(s) 553
Eco31I GGTCTC 1 cut(s) 534
Eco57I CTGAAG 1 cut(s) 294
Eco81I CCTNAGG 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 605
FaeI CATG 3 cut(s) 80, 459, 571
FaiI YATR 6 cut(s) 78, 117, 180, 375, 457, 569
FaqI GGGAC 1 cut(s) 394
FatI CATG 3 cut(s) 76, 455, 567
FauI CCCGC 2 cut(s) 278, 621
Fnu4HI GCNGC 3 cut(s) 131, 337, 340
FokI GGATG 1 cut(s) 590
Fsp4HI GCNGC 3 cut(s) 131, 337, 340
GluI GCNGC 3 cut(s) 131, 337, 340
GsuI CTGGAG 1 cut(s) 643
HapII CCGG 1 cut(s) 312
Hin1II CATG 3 cut(s) 80, 459, 571
HinfI GANTC 1 cut(s) 512
HpaII CCGG 1 cut(s) 312
HphI GGTGA 5 cut(s) 9, 421, 449, 595, 607
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 6 cut(s) 28, 106, 274, 326, 534, 647
Hpy188III TCNNGA 3 cut(s) 137, 392, 419
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 5 cut(s) 203, 235, 241, 269, 529
HpyCH4III ACNGT 2 cut(s) 453, 601
HpyCH4IV ACGT 2 cut(s) 300, 423
HpyCH4V TGCA 8 cut(s) 119, 130, 158, 339, 363, 432, 459, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 291, 336, 360, 618
HpyF3I CTNAG 4 cut(s) 103, 189, 474, 531
HpySE526I ACGT 2 cut(s) 300, 423
Hsp92II CATG 3 cut(s) 80, 459, 571
Kzo9I GATC 1 cut(s) 288
LmnI GCTCC 2 cut(s) 445, 566
LpnPI CCDG 8 cut(s) 246, 325, 377, 432, 509, 592, 607, 619
Lsp1109I GCAGC 3 cut(s) 142, 323, 351
MabI ACCWGGT 1 cut(s) 605
MaeII ACGT 2 cut(s) 300, 423
MaeIII GTNAC 2 cut(s) 595, 601
MalI GATC 1 cut(s) 290
MboI GATC 1 cut(s) 288
MboII GAAGA 2 cut(s) 32, 599
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 288
MhlI GDGCHC 2 cut(s) 614, 640
MluCI AATT 3 cut(s) 159, 387, 465
MmeI TCCRAC 1 cut(s) 423
MnlI CCTC 4 cut(s) 133, 163, 280, 583
MroXI GAANNNNTTC 1 cut(s) 93
MseI TTAA 2 cut(s) 170, 552
MslI CAYNNNNRTG 3 cut(s) 81, 454, 572
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 1 cut(s) 607
MunI CAATTG 1 cut(s) 159
MvaI CCWGG 1 cut(s) 607
MwoI GCNNNNNNNGC 4 cut(s) 291, 336, 360, 618
NdeII GATC 1 cut(s) 288
NlaIII CATG 3 cut(s) 80, 459, 571
NlaIV GGNNCC 2 cut(s) 447, 611
NmuCI GTSAC 2 cut(s) 595, 601
PdmI GAANNNNTTC 1 cut(s) 93
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 3 cut(s) 132, 338, 341
Psp6I CCWGG 1 cut(s) 605
PspGI CCWGG 1 cut(s) 605
PspN4I GGNNCC 2 cut(s) 447, 611
PstI CTGCAG 1 cut(s) 341
PstNI CAGNNNCTG 1 cut(s) 336
PsuI RGATCY 1 cut(s) 288
RseI CAYNNNNRTG 3 cut(s) 81, 454, 572
SaqAI TTAA 2 cut(s) 170, 552
SatI GCNGC 3 cut(s) 131, 337, 340
Sau3AI GATC 1 cut(s) 288
ScrFI CCNGG 1 cut(s) 607
SduI GDGCHC 2 cut(s) 614, 640
SexAI ACCWGGT 1 cut(s) 605
SfcI CTRYAG 1 cut(s) 337
SmiMI CAYNNNNRTG 3 cut(s) 81, 454, 572
Sse9I AATT 3 cut(s) 159, 387, 465
SsiI CCGC 3 cut(s) 285, 564, 614
StyD4I CCNGG 1 cut(s) 605
TaaI ACNGT 2 cut(s) 453, 601
TaiI ACGT 2 cut(s) 303, 426
TaqI TCGA 1 cut(s) 308
TasI AATT 3 cut(s) 159, 387, 465
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 2 cut(s) 170, 552
Tru9I TTAA 2 cut(s) 170, 552
TscAI CASTG 2 cut(s) 337, 411
TseFI GTSAC 2 cut(s) 595, 601
TseI GCWGC 3 cut(s) 130, 336, 339
Tsp45I GTSAC 2 cut(s) 595, 601
TspDTI ATGAA 3 cut(s) 102, 156, 245
TspRI CASTG 2 cut(s) 337, 411
XapI RAATTY 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 93
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.