In Between Ring fingers
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 834 |
| Acc36I | ACCTGC | 1 cut(s) | 834 |
| AccB1I | GGYRCC | 4 cut(s) | 786, 795, 846, 903 |
| AccII | CGCG | 2 cut(s) | 209, 404 |
| AccIII | TCCGGA | 1 cut(s) | 686 |
| AciI | CCGC | 2 cut(s) | 306, 402 |
| AclWI | GGATC | 1 cut(s) | 101 |
| AcyI | GRCGYC | 1 cut(s) | 170 |
| AfaI | GTAC | 1 cut(s) | 481 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 306, 445, 499 |
| AgsI | TTSAA | 1 cut(s) | 320 |
| AjnI | CCWGG | 1 cut(s) | 791 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 488, 520 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 657, 689 |
| AluBI | AGCT | 4 cut(s) | 227, 357, 420, 760 |
| AluI | AGCT | 4 cut(s) | 227, 357, 420, 760 |
| Alw21I | GWGCWC | 1 cut(s) | 364 |
| Alw26I | GTCTC | 2 cut(s) | 16, 915 |
| AlwI | GGATC | 1 cut(s) | 101 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 20 |
| Aor13HI | TCCGGA | 1 cut(s) | 686 |
| AspLEI | GCGC | 1 cut(s) | 662 |
| AsuC2I | CCSGG | 1 cut(s) | 441 |
| AsuHPI | GGTGA | 2 cut(s) | 124, 607 |
| AsuII | TTCGAA | 1 cut(s) | 507 |
| BaeGI | GKGCMC | 2 cut(s) | 638, 791 |
| BanI | GGYRCC | 4 cut(s) | 786, 795, 846, 903 |
| BbsI | GAAGAC | 3 cut(s) | 165, 207, 821 |
| Bbv12I | GWGCWC | 1 cut(s) | 364 |
| BccI | CCATC | 1 cut(s) | 265 |
| BciT130I | CCWGG | 1 cut(s) | 793 |
| BclI | TGATCA | 1 cut(s) | 82 |
| BcnI | CCSGG | 1 cut(s) | 441 |
| BcoDI | GTCTC | 2 cut(s) | 16, 915 |
| BfaI | CTAG | 1 cut(s) | 390 |
| BfuAI | ACCTGC | 1 cut(s) | 834 |
| BglII | AGATCT | 1 cut(s) | 742 |
| BisI | GCNGC | 1 cut(s) | 402 |
| BlsI | GCNGC | 1 cut(s) | 403 |
| Bme1390I | CCNGG | 2 cut(s) | 441, 793 |
| BmiI | GGNNCC | 5 cut(s) | 675, 788, 797, 848, 905 |
| BmrFI | CCNGG | 2 cut(s) | 441, 793 |
| BmsI | GCATC | 4 cut(s) | 111, 115, 511, 702 |
| BpiI | GAAGAC | 3 cut(s) | 165, 207, 821 |
| Bpu14I | TTCGAA | 1 cut(s) | 507 |
| BpuEI | CTTGAG | 2 cut(s) | 491, 804 |
| BpuMI | CCSGG | 1 cut(s) | 441 |
| Bsa29I | ATCGAT | 1 cut(s) | 105 |
| BsaBI | GATNNNNATC | 1 cut(s) | 101 |
| BsaHI | GRCGYC | 1 cut(s) | 170 |
| BsaJI | CCNNGG | 4 cut(s) | 439, 677, 791, 895 |
| BsaWI | WCCGGW | 2 cut(s) | 109, 686 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 761, 791 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 306, 445, 499 |
| Bse1I | ACTGG | 3 cut(s) | 261, 414, 907 |
| Bse8I | GATNNNNATC | 1 cut(s) | 101 |
| BseAI | TCCGGA | 1 cut(s) | 686 |
| BseBI | CCWGG | 1 cut(s) | 793 |
| BseCI | ATCGAT | 1 cut(s) | 105 |
| BseDI | CCNNGG | 4 cut(s) | 439, 677, 791, 895 |
| BseGI | GGATG | 3 cut(s) | 46, 257, 671 |
| BseJI | GATNNNNATC | 1 cut(s) | 101 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 306, 445, 499 |
| BseMII | CTCAG | 3 cut(s) | 27, 390, 926 |
| BseNI | ACTGG | 3 cut(s) | 261, 414, 907 |
| BseRI | GAGGAG | 1 cut(s) | 716 |
| BseSI | GKGCMC | 2 cut(s) | 638, 791 |
| Bsh1236I | CGCG | 2 cut(s) | 209, 404 |
| BshNI | GGYRCC | 4 cut(s) | 786, 795, 846, 903 |
| BshVI | ATCGAT | 1 cut(s) | 105 |
| BsiHKAI | GWGCWC | 1 cut(s) | 364 |
| BsiSI | CCGG | 4 cut(s) | 110, 441, 663, 687 |
| BslFI | GGGAC | 1 cut(s) | 239 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 306, 445, 499 |
| BsmAI | GTCTC | 2 cut(s) | 16, 915 |
| BsmFI | GGGAC | 1 cut(s) | 239 |
| BsmI | GAATGC | 1 cut(s) | 682 |
| Bsp119I | TTCGAA | 1 cut(s) | 507 |
| Bsp1286I | GDGCHC | 3 cut(s) | 364, 638, 791 |
| Bsp13I | TCCGGA | 1 cut(s) | 686 |
| Bsp143I | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| Bsp19I | CCATGG | 1 cut(s) | 895 |
| BspACI | CCGC | 2 cut(s) | 306, 402 |
| BspCNI | CTCAG | 3 cut(s) | 26, 389, 925 |
| BspDI | ATCGAT | 1 cut(s) | 105 |
| BspEI | TCCGGA | 1 cut(s) | 686 |
| BspFNI | CGCG | 2 cut(s) | 209, 404 |
| BspHI | TCATGA | 2 cut(s) | 70, 85 |
| BspLI | GGNNCC | 5 cut(s) | 675, 788, 797, 848, 905 |
| BspMI | ACCTGC | 1 cut(s) | 834 |
| BspPI | GGATC | 1 cut(s) | 101 |
| BspQI | GCTCTTC | 1 cut(s) | 30 |
| BspT104I | TTCGAA | 1 cut(s) | 507 |
| BspT107I | GGYRCC | 4 cut(s) | 786, 795, 846, 903 |
| BsrI | ACTGG | 3 cut(s) | 261, 414, 907 |
| BssECI | CCNNGG | 4 cut(s) | 439, 677, 791, 895 |
| BssMI | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| BssNI | GRCGYC | 1 cut(s) | 170 |
| BssT1I | CCWWGG | 1 cut(s) | 895 |
| Bst2UI | CCWGG | 1 cut(s) | 793 |
| Bst4CI | ACNGT | 1 cut(s) | 654 |
| Bst6I | CTCTTC | 2 cut(s) | 30, 776 |
| BstACI | GRCGYC | 1 cut(s) | 170 |
| BstBI | TTCGAA | 1 cut(s) | 507 |
| BstC8I | GCNNGC | 3 cut(s) | 244, 643, 762 |
| BstDEI | CTNAG | 3 cut(s) | 13, 376, 912 |
| BstDSI | CCRYGG | 2 cut(s) | 677, 895 |
| BstF5I | GGATG | 3 cut(s) | 46, 257, 671 |
| BstFNI | CGCG | 2 cut(s) | 209, 404 |
| BstHHI | GCGC | 1 cut(s) | 662 |
| BstKTI | GATC | 5 cut(s) | 37, 85, 109, 600, 745 |
| BstMAI | GTCTC | 2 cut(s) | 16, 915 |
| BstMBI | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 134, 167, 176, 248, 381, 642, 795 |
| BstNI | CCWGG | 1 cut(s) | 793 |
| BstSCI | CCNGG | 2 cut(s) | 439, 791 |
| BstSLI | GKGCMC | 2 cut(s) | 638, 791 |
| BstUI | CGCG | 2 cut(s) | 209, 404 |
| BstV2I | GAAGAC | 3 cut(s) | 165, 207, 821 |
| BstX2I | RGATCY | 1 cut(s) | 742 |
| BstYI | RGATCY | 1 cut(s) | 742 |
| Bsu15I | ATCGAT | 1 cut(s) | 105 |
| BsuTUI | ATCGAT | 1 cut(s) | 105 |
| BtgI | CCRYGG | 2 cut(s) | 677, 895 |
| BtgZI | GCGATG | 1 cut(s) | 190 |
| BtsCI | GGATG | 3 cut(s) | 46, 257, 671 |
| BtsI | GCAGTG | 1 cut(s) | 624 |
| BtsIMutI | CAGTG | 5 cut(s) | 59, 268, 580, 624, 920 |
| BveI | ACCTGC | 1 cut(s) | 834 |
| Cac8I | GCNNGC | 3 cut(s) | 244, 643, 762 |
| CaiI | CAGNNNCTG | 1 cut(s) | 20 |
| CciI | TCATGA | 2 cut(s) | 70, 85 |
| CfoI | GCGC | 1 cut(s) | 662 |
| ClaI | ATCGAT | 1 cut(s) | 105 |
| CseI | GACGC | 2 cut(s) | 159, 198 |
| Csp6I | GTAC | 1 cut(s) | 480 |
| CviAII | CATG | 4 cut(s) | 71, 86, 272, 896 |
| CviJI | RGCY | 9 cut(s) | 17, 161, 227, 246, 357, 401, 420, 536, 760 |
| CviKI_1 | RGCY | 9 cut(s) | 17, 161, 227, 246, 357, 401, 420, 536, 760 |
| CviQI | GTAC | 1 cut(s) | 480 |
| DdeI | CTNAG | 3 cut(s) | 13, 376, 912 |
| DpnI | GATC | 5 cut(s) | 36, 84, 108, 599, 744 |
| DpnII | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| Eam1104I | CTCTTC | 2 cut(s) | 30, 776 |
| EarI | CTCTTC | 2 cut(s) | 30, 776 |
| Eco130I | CCWWGG | 1 cut(s) | 895 |
| Eco32I | GATATC | 1 cut(s) | 120 |
| EcoRII | CCWGG | 1 cut(s) | 791 |
| EcoRV | GATATC | 1 cut(s) | 120 |
| EcoT14I | CCWWGG | 1 cut(s) | 895 |
| ErhI | CCWWGG | 1 cut(s) | 895 |
| FaeI | CATG | 4 cut(s) | 74, 89, 275, 899 |
| FaqI | GGGAC | 1 cut(s) | 239 |
| FatI | CATG | 4 cut(s) | 70, 85, 271, 895 |
| FauI | CCCGC | 1 cut(s) | 299 |
| FbaI | TGATCA | 1 cut(s) | 82 |
| Fnu4HI | GCNGC | 1 cut(s) | 402 |
| FokI | GGATG | 3 cut(s) | 53, 244, 678 |
| Fsp4HI | GCNGC | 1 cut(s) | 402 |
| FspBI | CTAG | 1 cut(s) | 390 |
| GlaI | GCGC | 1 cut(s) | 661 |
| GluI | GCNGC | 1 cut(s) | 402 |
| HapII | CCGG | 4 cut(s) | 110, 441, 663, 687 |
| HgaI | GACGC | 2 cut(s) | 159, 198 |
| HhaI | GCGC | 1 cut(s) | 662 |
| Hin1I | GRCGYC | 1 cut(s) | 170 |
| Hin1II | CATG | 4 cut(s) | 74, 89, 275, 899 |
| Hin6I | GCGC | 1 cut(s) | 660 |
| HinP1I | GCGC | 1 cut(s) | 660 |
| HinfI | GANTC | 5 cut(s) | 64, 74, 97, 626, 817 |
| HpaII | CCGG | 4 cut(s) | 110, 441, 663, 687 |
| HphI | GGTGA | 2 cut(s) | 124, 607 |
| Hpy188I | TCNGA | 6 cut(s) | 79, 124, 231, 379, 631, 711 |
| Hpy188III | TCNNGA | 6 cut(s) | 32, 38, 71, 86, 601, 687 |
| Hpy99I | CGWCG | 1 cut(s) | 214 |
| HpyAV | CCTTC | 3 cut(s) | 190, 379, 602 |
| HpyCH4III | ACNGT | 1 cut(s) | 654 |
| HpyCH4V | TGCA | 8 cut(s) | 148, 266, 384, 453, 764, 832, 843, 918 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 134, 167, 176, 248, 381, 642, 795 |
| HpyF3I | CTNAG | 3 cut(s) | 13, 376, 912 |
| Hsp92I | GRCGYC | 1 cut(s) | 170 |
| Hsp92II | CATG | 4 cut(s) | 74, 89, 275, 899 |
| HspAI | GCGC | 1 cut(s) | 660 |
| Kpn2I | TCCGGA | 1 cut(s) | 686 |
| Ksp22I | TGATCA | 1 cut(s) | 82 |
| Kzo9I | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| LguI | GCTCTTC | 1 cut(s) | 30 |
| LmnI | GCTCC | 1 cut(s) | 875 |
| LweI | GCATC | 4 cut(s) | 111, 115, 511, 702 |
| MaeI | CTAG | 1 cut(s) | 390 |
| MaeIII | GTNAC | 1 cut(s) | 890 |
| MalI | GATC | 5 cut(s) | 36, 84, 108, 599, 744 |
| MboI | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| MflI | RGATCY | 1 cut(s) | 742 |
| MhlI | GDGCHC | 3 cut(s) | 364, 638, 791 |
| MluCI | AATT | 5 cut(s) | 143, 486, 497, 851, 925 |
| MlyI | GAGTC | 3 cut(s) | 68, 635, 811 |
| MroI | TCCGGA | 1 cut(s) | 686 |
| MseI | TTAA | 2 cut(s) | 717, 928 |
| MslI | CAYNNNNRTG | 2 cut(s) | 90, 804 |
| MspI | CCGG | 4 cut(s) | 110, 441, 663, 687 |
| MspR9I | CCNGG | 2 cut(s) | 441, 793 |
| Mva1269I | GAATGC | 1 cut(s) | 682 |
| MvaI | CCWGG | 1 cut(s) | 793 |
| MvnI | CGCG | 2 cut(s) | 209, 404 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 134, 167, 176, 248, 381, 642, 795 |
| NciI | CCSGG | 1 cut(s) | 441 |
| NcoI | CCATGG | 1 cut(s) | 895 |
| NdeII | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| NlaIII | CATG | 4 cut(s) | 74, 89, 275, 899 |
| NlaIV | GGNNCC | 5 cut(s) | 675, 788, 797, 848, 905 |
| NspV | TTCGAA | 1 cut(s) | 507 |
| PagI | TCATGA | 2 cut(s) | 70, 85 |
| PaqCI | CACCTGC | 1 cut(s) | 834 |
| PciSI | GCTCTTC | 1 cut(s) | 30 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 218 |
| PctI | GAATGC | 1 cut(s) | 682 |
| PfeI | GAWTC | 2 cut(s) | 64, 97 |
| PkrI | GCNGC | 1 cut(s) | 403 |
| PleI | GAGTC | 3 cut(s) | 68, 634, 811 |
| PpsI | GAGTC | 3 cut(s) | 68, 634, 811 |
| Psp6I | CCWGG | 1 cut(s) | 791 |
| PspGI | CCWGG | 1 cut(s) | 791 |
| PspN4I | GGNNCC | 5 cut(s) | 675, 788, 797, 848, 905 |
| PstNI | CAGNNNCTG | 1 cut(s) | 20 |
| PsuI | RGATCY | 1 cut(s) | 742 |
| RsaI | GTAC | 1 cut(s) | 481 |
| RsaNI | GTAC | 1 cut(s) | 480 |
| RseI | CAYNNNNRTG | 2 cut(s) | 90, 804 |
| SapI | GCTCTTC | 1 cut(s) | 30 |
| SaqAI | TTAA | 2 cut(s) | 717, 928 |
| SatI | GCNGC | 1 cut(s) | 402 |
| Sau3AI | GATC | 5 cut(s) | 34, 82, 106, 597, 742 |
| SchI | GAGTC | 3 cut(s) | 68, 635, 811 |
| ScrFI | CCNGG | 2 cut(s) | 441, 793 |
| SduI | GDGCHC | 3 cut(s) | 364, 638, 791 |
| SfaNI | GCATC | 4 cut(s) | 111, 115, 511, 702 |
| SfuI | TTCGAA | 1 cut(s) | 507 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 90, 804 |
| SmlI | CTYRAG | 2 cut(s) | 470, 819 |
| SmoI | CTYRAG | 2 cut(s) | 470, 819 |
| Sse9I | AATT | 5 cut(s) | 143, 486, 497, 851, 925 |
| SsiI | CCGC | 2 cut(s) | 306, 402 |
| SspMI | CTAG | 1 cut(s) | 390 |
| StyD4I | CCNGG | 2 cut(s) | 439, 791 |
| StyI | CCWWGG | 1 cut(s) | 895 |
| TaaI | ACNGT | 1 cut(s) | 654 |
| TaqI | TCGA | 3 cut(s) | 105, 507, 600 |
| TasI | AATT | 5 cut(s) | 143, 486, 497, 851, 925 |
| TauI | GCSGC | 1 cut(s) | 404 |
| TfiI | GAWTC | 2 cut(s) | 64, 97 |
| Tru1I | TTAA | 2 cut(s) | 717, 928 |
| Tru9I | TTAA | 2 cut(s) | 717, 928 |
| TscAI | CASTG | 5 cut(s) | 66, 268, 580, 624, 920 |
| TspDTI | ATGAA | 5 cut(s) | 56, 102, 172, 288, 366 |
| TspGWI | ACGGA | 1 cut(s) | 666 |
| TspRI | CASTG | 5 cut(s) | 66, 268, 580, 624, 920 |
| XspI | CTAG | 1 cut(s) | 390 |