Rorug02G0401000

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000271
Physical Location & Seq
Forward (+)
51224772 .. 51228635
3864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0401000.1

Sequence Viewer

Length: 1005 bp
ATGCAAGCATTTTGTTGGGGTTTTGTCTTAATCACAGTGTCATACATTGTGCTCTCAGAGGATTCAAATTTCTTGGAGGATGAGAGCACAAAGATTTTTGCACGAAAAGTGGCCGATCATTATAGTGCAAGGACAAATCAAACTCTGGAAGAACGAGAAGCTAGTCCGGTCATCCATTTAAAAAAGCTTAACAACTGGATTAAGAGTGTCTTAATTCAGCTCTATGCTCACCGAGGAGATGCTGTTCTTGATCTTGCATGCGGCAAGGGAGGTGATCTCATCAAATGGGACAAGGCCAAAATTGGGTACTATGTTGGCATTGATATTGCTGAAGGCTCTATAGAAGATTGTCGTACCCGTTACAATGGTGATGCCGATCATCATCAATGCCGTAAAAAGTTTACATTTCCTGCATGCCTTATCTTTGGAGACTGCTATGAGGTTCGACTGGATAAAGCCCTAGCAGATGATACACCCTTTGATATTTGCAGTTGCCAGTTTGCCTTGCATTATTCCTGGTCCTCTAAAGCACGAGCACGGAGAGCCTTGGCTAATGTTTCAGCCTTACTTCGCCCGGGCGGAACTTTCATTGGAACAATGCCAGACGCTAATGTGATTATCAAAAAGCTGAGAGAAGCTGCAGGCTTATTCTTTGGAAATAGTGTCTACTGGATGCAATTCAATGAAGAATTTTCGGAAAAGGATGCTGTTGATTGCCCTGAATGGATCATCCCCTTTCATTATGATATGGAGTTAATTTTTGTGAAGAACTCTCATGAATTTGTGCACGAGTATATGAAAAGACCAGAATACGTAGAGTTAATGTGGAGGCTTGGTGCATTAGGTGACGGTAACCAAGATCAGAATGAATGGGAAGTAGCCTATTTGTACTCGTCATTTGTCTTGAGGAAGCGGGGCCAACCTGATTGGATTCAACCAAATGGTAGAACAGATAAAGGGAAGATGCATATATCAAAAGAGGACATATTGAACATTGAACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RING_UBOX

Protein Analysis

334

Amino Acids

38.46

Weight (kDa)

5.8

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mRNA_G-N7_MeTrfase PF03291 26 - 305 3.4e-97 mRNA (guanine-N(7))-methyltransferase domain
Methyltransf_31 PF13847 77 - 202 7.1e-08 Methyltransferase domain
Methyltransf_25 PF13649 82 - 194 6.2e-09 Methyltransferase domain
Methyltransf_12 PF08242 83 - 196 1.9e-09 Methyltransferase domain
Methyltransf_11 PF08241 83 - 197 7.9e-08 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14250
fragaria_vesca FvH4_1g09490 FvH4_1g09500 FvH4_1g09500 FvH4_1g09510 FvH4_3g00870 FvH4_3g03010 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g35700 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220
malus_domestica MD02G1102400.v1.1 MD02G1103700.v1.1 MD03G1111900.v1.1 MD09G1173800.v1.1 MD09G1230900.v1.1 MD09G1231400.v1.1 MD15G1224200.v1.1
prunus_persica Prupe.3G031900_v2.0.a1 Prupe.3G107200_v2.0.a1 Prupe.7G191200_v2.0.a1 Prupe.7G191800_v2.0.a1 Prupe.8G007700_v2.0.a1
pyrus_communis pycom02g08010 pycom02g08140 pycom09g14980 pycom09g15030 pycom15g19900
rosa_chinensis RchiOBHm_Chr2g0096041 RchiOBHm_Chr2g0096051 RchiOBHm_Chr2g0096061 RchiOBHm_Chr2g0096071 RchiOBHm_Chr2g0096081 RchiOBHm_Chr2g0096221 RchiOBHm_Chr2g0131511 RchiOBHm_Chr2g0147691 RchiOBHm_Chr5g0001281 RchiOBHm_Chr5g0001301
rosa_laevigata RLG00000016619 RLG00000016620 RLG00000016621 RLG00000016622 RLG00000019200 RLG00000020271 RLG00000025410 RLG00000030919 RLG00000031102 RLG00000031117 RLG00000031119 RLG00000031120
rosa_multiflora Rmu_co8160592.1_g000001 Rmu_sc0001706.1_g000008 Rmu_sc0002494.1_g000005 Rmu_sc0004189.1_g000040 Rmu_sc0005612.1_g000008 Rmu_sc0005612.1_g000011 Rmu_sc0005612.1_g000012 Rmu_sc0006926.1_g000015 Rmu_sc0013257.1_g000002 Rmu_sc0034363.1_g000001 Rmu_sc0034363.1_g000002
rosa_roxburghii Rroxscaffold_1G00072690 Rroxscaffold_1G00075020 Rroxscaffold_2G00145780 Rroxscaffold_2G00145790 Rroxscaffold_2G00145800 Rroxscaffold_2G00145810 Rroxscaffold_2G00145820 Rroxscaffold_2G00145830 Rroxscaffold_4G00300930 Rroxscaffold_6G00424750
rosa_rugosa Rorug02G0056000 Rorug02G0056100 Rorug02G0056200 Rorug02G0056300 Rorug02G0056500 Rorug02G0056700 Rorug02G0298000.1 Rorug02G0400900 Rorug02G0401000 Rorug02G0401100 Rorug04G0389500 Rorug04G0389600 Rorug04G0389700 Rorug04G0402300
rosa_samantha Rh2AG103800 Rh2AG458300 Rh2BG105400 Rh2BG105500 Rh2BG105600 Rh2BG105700 Rh2BG105900 Rh2BG357600 Rh2BG471000 Rh2DG106000 Rh2DG106100 Rh2DG106200 Rh2DG106300 Rh2DG106500 Rh2DG375400 Rh2DG480300 Rh5AG009500 Rh5AG009600 Rh5AG010000 Rh5AG028300 Rh5BG012400 Rh5BG012500 Rh5BG012700 Rh5BG028500
rosa_wichuraiana Rw2G007950 Rw2G007960 Rw2G007970 Rw2G028490 Rw5G000980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 666
AciI CCGC 3 cut(s) 261, 579, 913
AclWI GGATC 1 cut(s) 734
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 3 cut(s) 67, 689, 779
AcuI CTGAAG 1 cut(s) 351
AfaI GTAC 3 cut(s) 308, 355, 890
AfiI CCNNNNNNNGG 1 cut(s) 303
AgsI TTSAA 5 cut(s) 66, 682, 935, 991, 998
AjnI CCWGG 1 cut(s) 515
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 5 cut(s) 161, 187, 220, 628, 638
AluI AGCT 5 cut(s) 161, 187, 220, 628, 638
Alw21I GWGCWC 4 cut(s) 54, 89, 538, 789
Alw26I GTCTC 1 cut(s) 423
Alw44I GTGCAC 1 cut(s) 785
AlwI GGATC 1 cut(s) 734
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 3 cut(s) 111, 294, 916
ApaLI GTGCAC 1 cut(s) 785
ApeKI GCWGC 1 cut(s) 638
ApoI RAATTY 3 cut(s) 67, 689, 779
AspS9I GGNCC 2 cut(s) 519, 916
AsuC2I CCSGG 2 cut(s) 575, 576
AsuHPI GGTGA 4 cut(s) 221, 284, 380, 857
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 1 cut(s) 519
BaeGI GKGCMC 1 cut(s) 789
BauI CACGAG 2 cut(s) 531, 788
Bbv12I GWGCWC 4 cut(s) 54, 89, 538, 789
BbvI GCAGC 1 cut(s) 625
BceAI ACGGC 1 cut(s) 375
BciT130I CCWGG 1 cut(s) 517
BcnI CCSGG 2 cut(s) 575, 576
BcoDI GTCTC 1 cut(s) 423
BfaI CTAG 2 cut(s) 162, 461
BfmI CTRYAG 2 cut(s) 339, 639
BisI GCNGC 2 cut(s) 262, 639
BlsI GCNGC 2 cut(s) 263, 640
Bme1390I CCNGG 3 cut(s) 517, 575, 576
Bme18I GGWCC 1 cut(s) 519
BmeT110I CYCGRG 1 cut(s) 574
BmgT120I GGNCC 2 cut(s) 519, 916
BmiI GGNNCC 1 cut(s) 917
BmrFI CCNGG 3 cut(s) 517, 575, 576
BmsI GCATC 5 cut(s) 229, 361, 663, 694, 954
BplI GAGNNNNNCTC 2 cut(s) 261, 293
BpuEI CTTGAG 1 cut(s) 925
BpuMI CCSGG 2 cut(s) 575, 576
BsaAI YACGTR 1 cut(s) 814
BsaBI GATNNNNATC 2 cut(s) 375, 381
BsaJI CCNNGG 3 cut(s) 232, 546, 574
BsaWI WCCGGW 1 cut(s) 166
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 820, 850
Bsc4I CCNNNNNNNGG 1 cut(s) 303
Bse1I ACTGG 4 cut(s) 200, 453, 496, 674
Bse8I GATNNNNATC 2 cut(s) 375, 381
BseBI CCWGG 1 cut(s) 517
BseDI CCNNGG 3 cut(s) 232, 546, 574
BseGI GGATG 5 cut(s) 85, 171, 678, 709, 729
BseJI GATNNNNATC 2 cut(s) 375, 381
BseLI CCNNNNNNNGG 1 cut(s) 303
BseMII CTCAG 2 cut(s) 69, 620
BseNI ACTGG 4 cut(s) 200, 453, 496, 674
BseRI GAGGAG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 789
BseXI GCAGC 1 cut(s) 625
BshFI GGCC 3 cut(s) 113, 296, 918
BsiHKAI GWGCWC 4 cut(s) 54, 89, 538, 789
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 2 cut(s) 167, 575
BslFI GGGAC 1 cut(s) 302
BslI CCNNNNNNNGG 1 cut(s) 303
BsmAI GTCTC 1 cut(s) 423
BsmFI GGGAC 1 cut(s) 302
BsnI GGCC 3 cut(s) 113, 296, 918
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 4 cut(s) 54, 89, 538, 789
Bsp143I GATC 6 cut(s) 115, 250, 274, 376, 726, 859
BspACI CCGC 3 cut(s) 261, 579, 913
BspANI GGCC 3 cut(s) 113, 296, 918
BspCNI CTCAG 2 cut(s) 68, 621
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 1 cut(s) 917
BspMAI CTGCAG 1 cut(s) 643
BspPI GGATC 1 cut(s) 734
BsrI ACTGG 4 cut(s) 200, 453, 496, 674
BssECI CCNNGG 3 cut(s) 232, 546, 574
BssMI GATC 6 cut(s) 115, 250, 274, 376, 726, 859
BssSI CACGAG 2 cut(s) 531, 788
BssT1I CCWWGG 1 cut(s) 546
Bst2BI CACGAG 2 cut(s) 531, 788
Bst2UI CCWGG 1 cut(s) 517
Bst4CI ACNGT 2 cut(s) 37, 851
BstBAI YACGTR 1 cut(s) 814
BstC8I GCNNGC 4 cut(s) 6, 259, 415, 643
BstDEI CTNAG 2 cut(s) 55, 629
BstEII GGTNACC 1 cut(s) 851
BstF5I GGATG 5 cut(s) 85, 171, 678, 709, 729
BstKTI GATC 6 cut(s) 118, 253, 277, 379, 729, 862
BstMAI GTCTC 1 cut(s) 423
BstMBI GATC 6 cut(s) 115, 250, 274, 376, 726, 859
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNI CCWGG 1 cut(s) 517
BstNSI RCATGY 2 cut(s) 261, 417
BstPI GGTNACC 1 cut(s) 851
BstSCI CCNGG 3 cut(s) 515, 573, 574
BstSFI CTRYAG 2 cut(s) 339, 639
BstSLI GKGCMC 1 cut(s) 789
BstSNI TACGTA 1 cut(s) 814
BstV1I GCAGC 1 cut(s) 625
BsuRI GGCC 3 cut(s) 113, 296, 918
BtsCI GGATG 5 cut(s) 85, 171, 678, 709, 729
BtsIMutI CAGTG 1 cut(s) 42
Cac8I GCNNGC 4 cut(s) 6, 259, 415, 643
CciI TCATGA 1 cut(s) 775
Cfr13I GGNCC 2 cut(s) 519, 916
Cfr9I CCCGGG 1 cut(s) 574
CseI GACGC 1 cut(s) 614
Csp6I GTAC 3 cut(s) 307, 354, 889
CviAII CATG 3 cut(s) 258, 414, 776
CviQI GTAC 3 cut(s) 307, 354, 889
DdeI CTNAG 2 cut(s) 55, 629
DpnI GATC 6 cut(s) 117, 252, 276, 378, 728, 861
DpnII GATC 6 cut(s) 115, 250, 274, 376, 726, 859
DraI TTTAAA 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 111
EciI GGCGGA 1 cut(s) 594
Eco105I TACGTA 1 cut(s) 814
Eco130I CCWWGG 1 cut(s) 546
Eco47I GGWCC 1 cut(s) 519
Eco57I CTGAAG 1 cut(s) 351
Eco88I CYCGRG 1 cut(s) 574
Eco91I GGTNACC 1 cut(s) 851
EcoO65I GGTNACC 1 cut(s) 851
EcoRII CCWGG 1 cut(s) 515
EcoT14I CCWWGG 1 cut(s) 546
EcoT22I ATGCAT 1 cut(s) 969
ErhI CCWWGG 1 cut(s) 546
FaeI CATG 3 cut(s) 261, 417, 779
FalI AAGNNNNNCTT 2 cut(s) 194, 226
FaqI GGGAC 1 cut(s) 302
FatI CATG 3 cut(s) 257, 413, 775
FauI CCCGC 1 cut(s) 906
FblI GTMKAC 1 cut(s) 666
Fnu4HI GCNGC 2 cut(s) 262, 639
FokI GGATG 5 cut(s) 92, 158, 685, 716, 716
Fsp4HI GCNGC 2 cut(s) 262, 639
FspBI CTAG 2 cut(s) 162, 461
GluI GCNGC 2 cut(s) 262, 639
HaeIII GGCC 3 cut(s) 113, 296, 918
HapII CCGG 2 cut(s) 167, 575
HgaI GACGC 1 cut(s) 614
Hin1II CATG 3 cut(s) 261, 417, 779
HindIII AAGCTT 1 cut(s) 185
HinfI GANTC 2 cut(s) 62, 931
HpaII CCGG 2 cut(s) 167, 575
HphI GGTGA 4 cut(s) 221, 284, 380, 857
Hpy166II GTNNAC 3 cut(s) 402, 667, 787
Hpy188I TCNGA 3 cut(s) 58, 697, 864
Hpy188III TCNNGA 4 cut(s) 146, 248, 776, 904
Hpy8I GTNNAC 3 cut(s) 402, 667, 787
HpyAV CCTTC 1 cut(s) 326
HpyCH4III ACNGT 2 cut(s) 37, 851
HpyCH4IV ACGT 1 cut(s) 813
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 2 cut(s) 55, 629
HpySE526I ACGT 1 cut(s) 813
Hsp92II CATG 3 cut(s) 261, 417, 779
Kzo9I GATC 6 cut(s) 115, 250, 274, 376, 726, 859
Lsp1109I GCAGC 1 cut(s) 625
LweI GCATC 5 cut(s) 229, 361, 663, 694, 954
MaeI CTAG 2 cut(s) 162, 461
MaeII ACGT 1 cut(s) 813
MaeIII GTNAC 3 cut(s) 359, 845, 851
MalI GATC 6 cut(s) 117, 252, 276, 378, 728, 861
MboI GATC 6 cut(s) 115, 250, 274, 376, 726, 859
MboII GAAGA 5 cut(s) 161, 356, 698, 778, 973
MhlI GDGCHC 4 cut(s) 54, 89, 538, 789
MluCI AATT 7 cut(s) 67, 213, 300, 677, 689, 756, 779
MnlI CCTC 9 cut(s) 52, 70, 227, 263, 433, 532, 822, 900, 973
Mph1103I ATGCAT 1 cut(s) 969
MseI TTAA 7 cut(s) 29, 179, 189, 201, 212, 755, 821
MslI CAYNNNNRTG 1 cut(s) 123
MspI CCGG 2 cut(s) 167, 575
MspR9I CCNGG 3 cut(s) 517, 575, 576
MvaI CCWGG 1 cut(s) 517
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 2 cut(s) 575, 576
NdeII GATC 6 cut(s) 115, 250, 274, 376, 726, 859
NlaIII CATG 3 cut(s) 261, 417, 779
NlaIV GGNNCC 1 cut(s) 917
NmuCI GTSAC 1 cut(s) 845
NsiI ATGCAT 1 cut(s) 969
NspI RCATGY 2 cut(s) 261, 417
PaeI GCATGC 2 cut(s) 261, 417
PagI TCATGA 1 cut(s) 775
PfeI GAWTC 2 cut(s) 62, 931
PkrI GCNGC 2 cut(s) 263, 640
Ppu21I YACGTR 1 cut(s) 814
Psp6I CCWGG 1 cut(s) 515
PspEI GGTNACC 1 cut(s) 851
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 917
PspPI GGNCC 2 cut(s) 519, 916
PstI CTGCAG 1 cut(s) 643
RsaI GTAC 3 cut(s) 308, 355, 890
RsaNI GTAC 3 cut(s) 307, 354, 889
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 7 cut(s) 29, 179, 189, 201, 212, 755, 821
SatI GCNGC 2 cut(s) 262, 639
Sau3AI GATC 6 cut(s) 115, 250, 274, 376, 726, 859
Sau96I GGNCC 2 cut(s) 519, 916
ScrFI CCNGG 3 cut(s) 517, 575, 576
SduI GDGCHC 4 cut(s) 54, 89, 538, 789
SfaNI GCATC 5 cut(s) 229, 361, 663, 694, 954
SfcI CTRYAG 2 cut(s) 339, 639
SinI GGWCC 1 cut(s) 519
SmaI CCCGGG 1 cut(s) 576
SmiMI CAYNNNNRTG 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 904
SmoI CTYRAG 1 cut(s) 904
SnaBI TACGTA 1 cut(s) 814
SphI GCATGC 2 cut(s) 261, 417
SrfI GCCCGGGC 1 cut(s) 576
Sse9I AATT 7 cut(s) 67, 213, 300, 677, 689, 756, 779
SsiI CCGC 3 cut(s) 261, 579, 913
SspMI CTAG 2 cut(s) 162, 461
StyD4I CCNGG 3 cut(s) 515, 573, 574
StyI CCWWGG 1 cut(s) 546
TaaI ACNGT 2 cut(s) 37, 851
TaiI ACGT 1 cut(s) 816
TaqI TCGA 1 cut(s) 445
TasI AATT 7 cut(s) 67, 213, 300, 677, 689, 756, 779
TatI WGTACW 1 cut(s) 888
TauI GCSGC 1 cut(s) 264
TfiI GAWTC 2 cut(s) 62, 931
Tru1I TTAA 7 cut(s) 29, 179, 189, 201, 212, 755, 821
Tru9I TTAA 7 cut(s) 29, 179, 189, 201, 212, 755, 821
TscAI CASTG 1 cut(s) 42
TseFI GTSAC 1 cut(s) 845
TseI GCWGC 1 cut(s) 638
Tsp45I GTSAC 1 cut(s) 845
TspDTI ATGAA 6 cut(s) 577, 699, 728, 792, 812, 882
TspGWI ACGGA 1 cut(s) 553
TspMI CCCGGG 1 cut(s) 574
TspRI CASTG 1 cut(s) 42
VneI GTGCAC 1 cut(s) 785
VpaK11BI GGWCC 1 cut(s) 519
XapI RAATTY 3 cut(s) 67, 689, 779
XceI RCATGY 2 cut(s) 261, 417
XmaI CCCGGG 1 cut(s) 574
XmiI GTMKAC 1 cut(s) 666
XspI CTAG 2 cut(s) 162, 461
Zsp2I ATGCAT 1 cut(s) 969
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.