E3 ubiquitin-protein ligase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 824 |
| AciI | CCGC | 1 cut(s) | 163 |
| AclWI | GGATC | 1 cut(s) | 699 |
| AcoI | YGGCCR | 1 cut(s) | 884 |
| AcsI | RAATTY | 1 cut(s) | 34 |
| AcuI | CTGAAG | 1 cut(s) | 219 |
| AflII | CTTAAG | 1 cut(s) | 449 |
| AflIII | ACRYGT | 1 cut(s) | 849 |
| AgsI | TTSAA | 3 cut(s) | 515, 642, 910 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 293, 325 |
| AluBI | AGCT | 7 cut(s) | 322, 346, 476, 497, 656, 793, 840 |
| AluI | AGCT | 7 cut(s) | 322, 346, 476, 497, 656, 793, 840 |
| Alw21I | GWGCWC | 1 cut(s) | 358 |
| Alw26I | GTCTC | 2 cut(s) | 448, 572 |
| AlwI | GGATC | 1 cut(s) | 699 |
| AoxI | GGCC | 4 cut(s) | 243, 740, 785, 884 |
| ApeKI | GCWGC | 3 cut(s) | 343, 367, 594 |
| ApoI | RAATTY | 1 cut(s) | 34 |
| AspA2I | CCTAGG | 1 cut(s) | 154 |
| AsuC2I | CCSGG | 1 cut(s) | 181 |
| AsuHPI | GGTGA | 1 cut(s) | 47 |
| AvrII | CCTAGG | 1 cut(s) | 154 |
| BaeGI | GKGCMC | 1 cut(s) | 197 |
| BalI | TGGCCA | 1 cut(s) | 886 |
| BbsI | GAAGAC | 2 cut(s) | 293, 422 |
| Bbv12I | GWGCWC | 1 cut(s) | 358 |
| BbvI | GCAGC | 3 cut(s) | 355, 379, 606 |
| BccI | CCATC | 4 cut(s) | 10, 227, 476, 784 |
| BclI | TGATCA | 1 cut(s) | 868 |
| BcnI | CCSGG | 1 cut(s) | 181 |
| BcoDI | GTCTC | 2 cut(s) | 448, 572 |
| BfaI | CTAG | 2 cut(s) | 155, 351 |
| BfmI | CTRYAG | 2 cut(s) | 365, 618 |
| BfrI | CTTAAG | 1 cut(s) | 449 |
| BisI | GCNGC | 3 cut(s) | 344, 368, 595 |
| BlnI | CCTAGG | 1 cut(s) | 154 |
| BlsI | GCNGC | 3 cut(s) | 345, 369, 596 |
| Bme1390I | CCNGG | 1 cut(s) | 181 |
| BmrFI | CCNGG | 1 cut(s) | 181 |
| BmsI | GCATC | 2 cut(s) | 155, 454 |
| BpiI | GAAGAC | 2 cut(s) | 293, 422 |
| BpmI | CTGGAG | 1 cut(s) | 636 |
| Bpu10I | CCTNAGC | 1 cut(s) | 789 |
| BpuEI | CTTGAG | 4 cut(s) | 111, 314, 344, 862 |
| BpuMI | CCSGG | 1 cut(s) | 181 |
| BsaJI | CCNNGG | 2 cut(s) | 154, 887 |
| Bse1I | ACTGG | 1 cut(s) | 10 |
| BseDI | CCNNGG | 2 cut(s) | 154, 887 |
| BseGI | GGATG | 1 cut(s) | 21 |
| BseMII | CTCAG | 3 cut(s) | 321, 780, 821 |
| BseNI | ACTGG | 1 cut(s) | 10 |
| BseRI | GAGGAG | 3 cut(s) | 42, 277, 639 |
| BseSI | GKGCMC | 1 cut(s) | 197 |
| BseXI | GCAGC | 3 cut(s) | 355, 379, 606 |
| BsgI | GTGCAG | 1 cut(s) | 103 |
| BshFI | GGCC | 4 cut(s) | 245, 742, 787, 886 |
| BsiHKAI | GWGCWC | 1 cut(s) | 358 |
| BsiSI | CCGG | 2 cut(s) | 180, 246 |
| BsmAI | GTCTC | 2 cut(s) | 448, 572 |
| BsnI | GGCC | 4 cut(s) | 245, 742, 787, 886 |
| Bsp1286I | GDGCHC | 2 cut(s) | 197, 358 |
| Bsp143I | GATC | 3 cut(s) | 64, 704, 868 |
| Bsp19I | CCATGG | 1 cut(s) | 887 |
| BspACI | CCGC | 1 cut(s) | 163 |
| BspANI | GGCC | 4 cut(s) | 245, 742, 787, 886 |
| BspCNI | CTCAG | 3 cut(s) | 322, 781, 820 |
| BspHI | TCATGA | 1 cut(s) | 70 |
| BspMAI | CTGCAG | 2 cut(s) | 369, 622 |
| BspPI | GGATC | 1 cut(s) | 699 |
| BspQI | GCTCTTC | 1 cut(s) | 397 |
| BspTI | CTTAAG | 1 cut(s) | 449 |
| BsrI | ACTGG | 1 cut(s) | 10 |
| BssECI | CCNNGG | 2 cut(s) | 154, 887 |
| BssMI | GATC | 3 cut(s) | 64, 704, 868 |
| BssT1I | CCWWGG | 2 cut(s) | 154, 887 |
| Bst4CI | ACNGT | 1 cut(s) | 459 |
| Bst6I | CTCTTC | 3 cut(s) | 195, 254, 397 |
| BstAFI | CTTAAG | 1 cut(s) | 449 |
| BstC8I | GCNNGC | 1 cut(s) | 348 |
| BstDEI | CTNAG | 3 cut(s) | 330, 789, 807 |
| BstDSI | CCRYGG | 1 cut(s) | 887 |
| BstF5I | GGATG | 1 cut(s) | 21 |
| BstKTI | GATC | 3 cut(s) | 67, 707, 871 |
| BstMAI | GTCTC | 2 cut(s) | 448, 572 |
| BstMBI | GATC | 3 cut(s) | 64, 704, 868 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 473 |
| BstNSI | RCATGY | 1 cut(s) | 853 |
| BstSCI | CCNGG | 1 cut(s) | 179 |
| BstSFI | CTRYAG | 2 cut(s) | 365, 618 |
| BstSLI | GKGCMC | 1 cut(s) | 197 |
| BstV1I | GCAGC | 3 cut(s) | 355, 379, 606 |
| BstV2I | GAAGAC | 2 cut(s) | 293, 422 |
| BstX2I | RGATCY | 1 cut(s) | 704 |
| BstYI | RGATCY | 1 cut(s) | 704 |
| BsuRI | GGCC | 4 cut(s) | 245, 742, 787, 886 |
| BtgI | CCRYGG | 1 cut(s) | 887 |
| BtsCI | GGATG | 1 cut(s) | 21 |
| BtsIMutI | CAGTG | 1 cut(s) | 195 |
| Cac8I | GCNNGC | 1 cut(s) | 348 |
| CciI | TCATGA | 1 cut(s) | 70 |
| CviAII | CATG | 7 cut(s) | 40, 71, 137, 209, 274, 850, 888 |
| DdeI | CTNAG | 3 cut(s) | 330, 789, 807 |
| DpnI | GATC | 3 cut(s) | 66, 706, 870 |
| DpnII | GATC | 3 cut(s) | 64, 704, 868 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 824 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 824 |
| EaeI | YGGCCR | 1 cut(s) | 884 |
| Eam1104I | CTCTTC | 3 cut(s) | 195, 254, 397 |
| EarI | CTCTTC | 3 cut(s) | 195, 254, 397 |
| Eco130I | CCWWGG | 2 cut(s) | 154, 887 |
| Eco147I | AGGCCT | 2 cut(s) | 742, 787 |
| Eco57I | CTGAAG | 1 cut(s) | 219 |
| EcoT14I | CCWWGG | 2 cut(s) | 154, 887 |
| ErhI | CCWWGG | 2 cut(s) | 154, 887 |
| FaeI | CATG | 7 cut(s) | 43, 74, 140, 212, 277, 853, 891 |
| FatI | CATG | 7 cut(s) | 39, 70, 136, 208, 273, 849, 887 |
| FbaI | TGATCA | 1 cut(s) | 868 |
| Fnu4HI | GCNGC | 3 cut(s) | 344, 368, 595 |
| FokI | GGATG | 1 cut(s) | 28 |
| Fsp4HI | GCNGC | 3 cut(s) | 344, 368, 595 |
| FspBI | CTAG | 2 cut(s) | 155, 351 |
| GluI | GCNGC | 3 cut(s) | 344, 368, 595 |
| GsuI | CTGGAG | 1 cut(s) | 636 |
| HaeIII | GGCC | 4 cut(s) | 245, 742, 787, 886 |
| HapII | CCGG | 2 cut(s) | 180, 246 |
| Hin1II | CATG | 7 cut(s) | 43, 74, 140, 212, 277, 853, 891 |
| HindIII | AAGCTT | 2 cut(s) | 320, 838 |
| HinfI | GANTC | 3 cut(s) | 100, 485, 554 |
| HpaII | CCGG | 2 cut(s) | 180, 246 |
| HphI | GGTGA | 1 cut(s) | 47 |
| Hpy188I | TCNGA | 4 cut(s) | 99, 229, 396, 810 |
| Hpy188III | TCNNGA | 4 cut(s) | 71, 128, 642, 747 |
| HpyAV | CCTTC | 2 cut(s) | 123, 753 |
| HpyCH4III | ACNGT | 1 cut(s) | 459 |
| HpyCH4V | TGCA | 6 cut(s) | 120, 146, 343, 367, 594, 620 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 473 |
| HpyF3I | CTNAG | 3 cut(s) | 330, 789, 807 |
| Hsp92II | CATG | 7 cut(s) | 43, 74, 140, 212, 277, 853, 891 |
| Ksp22I | TGATCA | 1 cut(s) | 868 |
| Kzo9I | GATC | 3 cut(s) | 64, 704, 868 |
| LguI | GCTCTTC | 1 cut(s) | 397 |
| LmnI | GCTCC | 1 cut(s) | 277 |
| Lsp1109I | GCAGC | 3 cut(s) | 355, 379, 606 |
| LweI | GCATC | 2 cut(s) | 155, 454 |
| MaeI | CTAG | 2 cut(s) | 155, 351 |
| MaeIII | GTNAC | 1 cut(s) | 237 |
| MalI | GATC | 3 cut(s) | 66, 706, 870 |
| MboI | GATC | 3 cut(s) | 64, 704, 868 |
| MfeI | CAATTG | 1 cut(s) | 910 |
| MflI | RGATCY | 1 cut(s) | 704 |
| MhlI | GDGCHC | 2 cut(s) | 197, 358 |
| MlsI | TGGCCA | 1 cut(s) | 886 |
| MluCI | AATT | 4 cut(s) | 34, 690, 905, 910 |
| MluNI | TGGCCA | 1 cut(s) | 886 |
| MmeI | TCCRAC | 3 cut(s) | 408, 612, 873 |
| Mox20I | TGGCCA | 1 cut(s) | 886 |
| MscI | TGGCCA | 1 cut(s) | 886 |
| MseI | TTAA | 1 cut(s) | 450 |
| Msp20I | TGGCCA | 1 cut(s) | 886 |
| MspA1I | CMGCKG | 1 cut(s) | 346 |
| MspCI | CTTAAG | 1 cut(s) | 449 |
| MspI | CCGG | 2 cut(s) | 180, 246 |
| MspR9I | CCNGG | 1 cut(s) | 181 |
| MunI | CAATTG | 1 cut(s) | 910 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 473 |
| NciI | CCSGG | 1 cut(s) | 181 |
| NcoI | CCATGG | 1 cut(s) | 887 |
| NdeII | GATC | 3 cut(s) | 64, 704, 868 |
| NlaIII | CATG | 7 cut(s) | 43, 74, 140, 212, 277, 853, 891 |
| NmuCI | GTSAC | 1 cut(s) | 237 |
| NspI | RCATGY | 1 cut(s) | 853 |
| PagI | TCATGA | 1 cut(s) | 70 |
| PceI | AGGCCT | 2 cut(s) | 742, 787 |
| PciI | ACATGT | 1 cut(s) | 849 |
| PciSI | GCTCTTC | 1 cut(s) | 397 |
| PfeI | GAWTC | 3 cut(s) | 100, 485, 554 |
| PfoI | TCCNGGA | 1 cut(s) | 179 |
| PkrI | GCNGC | 3 cut(s) | 345, 369, 596 |
| PscI | ACATGT | 1 cut(s) | 849 |
| PstI | CTGCAG | 2 cut(s) | 369, 622 |
| PsuI | RGATCY | 1 cut(s) | 704 |
| PvuII | CAGCTG | 1 cut(s) | 346 |
| SapI | GCTCTTC | 1 cut(s) | 397 |
| SaqAI | TTAA | 1 cut(s) | 450 |
| SatI | GCNGC | 3 cut(s) | 344, 368, 595 |
| Sau3AI | GATC | 3 cut(s) | 64, 704, 868 |
| ScrFI | CCNGG | 1 cut(s) | 181 |
| SduI | GDGCHC | 2 cut(s) | 197, 358 |
| SfaNI | GCATC | 2 cut(s) | 155, 454 |
| SfcI | CTRYAG | 2 cut(s) | 365, 618 |
| SmlI | CTYRAG | 5 cut(s) | 126, 293, 323, 449, 841 |
| SmoI | CTYRAG | 5 cut(s) | 126, 293, 323, 449, 841 |
| Sse9I | AATT | 4 cut(s) | 34, 690, 905, 910 |
| SseBI | AGGCCT | 2 cut(s) | 742, 787 |
| SsiI | CCGC | 1 cut(s) | 163 |
| SspMI | CTAG | 2 cut(s) | 155, 351 |
| StuI | AGGCCT | 2 cut(s) | 742, 787 |
| StyD4I | CCNGG | 1 cut(s) | 179 |
| StyI | CCWWGG | 2 cut(s) | 154, 887 |
| TaaI | ACNGT | 1 cut(s) | 459 |
| TasI | AATT | 4 cut(s) | 34, 690, 905, 910 |
| TfiI | GAWTC | 3 cut(s) | 100, 485, 554 |
| Tru1I | TTAA | 1 cut(s) | 450 |
| Tru9I | TTAA | 1 cut(s) | 450 |
| TscAI | CASTG | 1 cut(s) | 202 |
| TseFI | GTSAC | 1 cut(s) | 237 |
| TseI | GCWGC | 3 cut(s) | 343, 367, 594 |
| Tsp45I | GTSAC | 1 cut(s) | 237 |
| TspDTI | ATGAA | 4 cut(s) | 28, 125, 498, 755 |
| TspRI | CASTG | 1 cut(s) | 202 |
| Vha464I | CTTAAG | 1 cut(s) | 449 |
| XapI | RAATTY | 1 cut(s) | 34 |
| XceI | RCATGY | 1 cut(s) | 853 |
| XmaJI | CCTAGG | 1 cut(s) | 154 |
| XspI | CTAG | 2 cut(s) | 155, 351 |