In Between Ring fingers
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 873 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 600 |
| Acc36I | ACCTGC | 1 cut(s) | 873 |
| AccB1I | GGYRCC | 3 cut(s) | 586, 825, 885 |
| AccII | CGCG | 1 cut(s) | 23 |
| AciI | CCGC | 4 cut(s) | 21, 66, 779, 823 |
| AclWI | GGATC | 1 cut(s) | 789 |
| AfaI | GTAC | 1 cut(s) | 931 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 342, 481 |
| AjnI | CCWGG | 1 cut(s) | 724 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 441, 473 |
| AluBI | AGCT | 1 cut(s) | 572 |
| AluI | AGCT | 1 cut(s) | 572 |
| Alw21I | GWGCWC | 1 cut(s) | 703 |
| Alw44I | GTGCAC | 1 cut(s) | 699 |
| AlwI | GGATC | 1 cut(s) | 789 |
| Ama87I | CYCGRG | 1 cut(s) | 946 |
| ApaLI | GTGCAC | 1 cut(s) | 699 |
| ApeKI | GCWGC | 1 cut(s) | 393 |
| AspA2I | CCTAGG | 1 cut(s) | 830 |
| AspS9I | GGNCC | 1 cut(s) | 924 |
| AsuC2I | CCSGG | 1 cut(s) | 477 |
| AsuHPI | GGTGA | 3 cut(s) | 41, 163, 659 |
| AsuII | TTCGAA | 1 cut(s) | 382 |
| AvaI | CYCGRG | 1 cut(s) | 946 |
| AvaII | GGWCC | 1 cut(s) | 924 |
| AvrII | CCTAGG | 1 cut(s) | 830 |
| BaeGI | GKGCMC | 4 cut(s) | 476, 589, 703, 830 |
| BanI | GGYRCC | 3 cut(s) | 586, 825, 885 |
| BbsI | GAAGAC | 1 cut(s) | 860 |
| Bbv12I | GWGCWC | 1 cut(s) | 703 |
| BbvI | GCAGC | 1 cut(s) | 380 |
| BccI | CCATC | 2 cut(s) | 261, 331 |
| BciT130I | CCWGG | 1 cut(s) | 726 |
| BcnI | CCSGG | 1 cut(s) | 477 |
| BfaI | CTAG | 1 cut(s) | 831 |
| BfmI | CTRYAG | 1 cut(s) | 487 |
| BfuAI | ACCTGC | 1 cut(s) | 873 |
| BisI | GCNGC | 1 cut(s) | 394 |
| BlnI | CCTAGG | 1 cut(s) | 830 |
| BlsI | GCNGC | 1 cut(s) | 395 |
| BmcAI | AGTACT | 1 cut(s) | 931 |
| Bme1390I | CCNGG | 2 cut(s) | 477, 726 |
| Bme18I | GGWCC | 1 cut(s) | 924 |
| BmeT110I | CYCGRG | 1 cut(s) | 946 |
| BmgT120I | GGNCC | 1 cut(s) | 924 |
| BmiI | GGNNCC | 4 cut(s) | 199, 588, 827, 887 |
| BmrFI | CCNGG | 2 cut(s) | 477, 726 |
| BmsI | GCATC | 4 cut(s) | 446, 499, 547, 730 |
| BpiI | GAAGAC | 1 cut(s) | 860 |
| Bpu14I | TTCGAA | 1 cut(s) | 382 |
| BpuEI | CTTGAG | 2 cut(s) | 314, 843 |
| BpuMI | CCSGG | 1 cut(s) | 477 |
| BsaAI | YACGTR | 1 cut(s) | 463 |
| BsaBI | GATNNNNATC | 1 cut(s) | 36 |
| BsaJI | CCNNGG | 3 cut(s) | 475, 716, 830 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 342, 481 |
| Bse1I | ACTGG | 3 cut(s) | 104, 895, 927 |
| Bse8I | GATNNNNATC | 1 cut(s) | 36 |
| BseBI | CCWGG | 1 cut(s) | 726 |
| BseDI | CCNNGG | 3 cut(s) | 475, 716, 830 |
| BseGI | GGATG | 4 cut(s) | 253, 437, 721, 914 |
| BseJI | GATNNNNATC | 1 cut(s) | 36 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 342, 481 |
| BseMII | CTCAG | 1 cut(s) | 90 |
| BseNI | ACTGG | 3 cut(s) | 104, 895, 927 |
| BseRI | GAGGAG | 2 cut(s) | 665, 755 |
| BseSI | GKGCMC | 4 cut(s) | 476, 589, 703, 830 |
| BseXI | GCAGC | 1 cut(s) | 380 |
| Bsh1236I | CGCG | 1 cut(s) | 23 |
| BshNI | GGYRCC | 3 cut(s) | 586, 825, 885 |
| BsiHKAI | GWGCWC | 1 cut(s) | 703 |
| BsiHKCI | CYCGRG | 1 cut(s) | 946 |
| BsiSI | CCGG | 2 cut(s) | 300, 477 |
| BslFI | GGGAC | 2 cut(s) | 660, 698 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 342, 481 |
| BsmFI | GGGAC | 2 cut(s) | 660, 698 |
| BsoBI | CYCGRG | 1 cut(s) | 946 |
| Bsp119I | TTCGAA | 1 cut(s) | 382 |
| Bsp1286I | GDGCHC | 4 cut(s) | 476, 589, 703, 830 |
| Bsp143I | GATC | 3 cut(s) | 31, 633, 781 |
| BspACI | CCGC | 4 cut(s) | 21, 66, 779, 823 |
| BspCNI | CTCAG | 1 cut(s) | 91 |
| BspFNI | CGCG | 1 cut(s) | 23 |
| BspLI | GGNNCC | 4 cut(s) | 199, 588, 827, 887 |
| BspMAI | CTGCAG | 1 cut(s) | 491 |
| BspMI | ACCTGC | 1 cut(s) | 873 |
| BspPI | GGATC | 1 cut(s) | 789 |
| BspT104I | TTCGAA | 1 cut(s) | 382 |
| BspT107I | GGYRCC | 3 cut(s) | 586, 825, 885 |
| BsrI | ACTGG | 3 cut(s) | 104, 895, 927 |
| BssECI | CCNNGG | 3 cut(s) | 475, 716, 830 |
| BssMI | GATC | 3 cut(s) | 31, 633, 781 |
| BssT1I | CCWWGG | 2 cut(s) | 716, 830 |
| Bst2UI | CCWGG | 1 cut(s) | 726 |
| Bst4CI | ACNGT | 4 cut(s) | 197, 602, 625, 693 |
| Bst6I | CTCTTC | 2 cut(s) | 166, 208 |
| BstBAI | YACGTR | 1 cut(s) | 463 |
| BstBI | TTCGAA | 1 cut(s) | 382 |
| BstDEI | CTNAG | 1 cut(s) | 99 |
| BstF5I | GGATG | 4 cut(s) | 253, 437, 721, 914 |
| BstFNI | CGCG | 1 cut(s) | 23 |
| BstKTI | GATC | 3 cut(s) | 34, 636, 784 |
| BstMBI | GATC | 3 cut(s) | 31, 633, 781 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 239, 417, 486, 834 |
| BstNI | CCWGG | 1 cut(s) | 726 |
| BstNSI | RCATGY | 1 cut(s) | 434 |
| BstSCI | CCNGG | 2 cut(s) | 475, 724 |
| BstSFI | CTRYAG | 1 cut(s) | 487 |
| BstSLI | GKGCMC | 4 cut(s) | 476, 589, 703, 830 |
| BstUI | CGCG | 1 cut(s) | 23 |
| BstV1I | GCAGC | 1 cut(s) | 380 |
| BstV2I | GAAGAC | 1 cut(s) | 860 |
| BstX2I | RGATCY | 1 cut(s) | 781 |
| BstYI | RGATCY | 1 cut(s) | 781 |
| BtsCI | GGATG | 4 cut(s) | 253, 437, 721, 914 |
| BveI | ACCTGC | 1 cut(s) | 873 |
| Cfr13I | GGNCC | 1 cut(s) | 924 |
| CseI | GACGC | 2 cut(s) | 12, 193 |
| Csp6I | GTAC | 1 cut(s) | 930 |
| CviAII | CATG | 3 cut(s) | 263, 431, 935 |
| CviJI | RGCY | 9 cut(s) | 103, 149, 368, 393, 480, 494, 572, 656, 754 |
| CviKI_1 | RGCY | 9 cut(s) | 103, 149, 368, 393, 480, 494, 572, 656, 754 |
| CviQI | GTAC | 1 cut(s) | 930 |
| DdeI | CTNAG | 1 cut(s) | 99 |
| DpnI | GATC | 3 cut(s) | 33, 635, 783 |
| DpnII | GATC | 3 cut(s) | 31, 633, 781 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 600 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 600 |
| Eam1104I | CTCTTC | 2 cut(s) | 166, 208 |
| EarI | CTCTTC | 2 cut(s) | 166, 208 |
| EciI | GGCGGA | 1 cut(s) | 794 |
| Eco130I | CCWWGG | 2 cut(s) | 716, 830 |
| Eco47I | GGWCC | 1 cut(s) | 924 |
| Eco88I | CYCGRG | 1 cut(s) | 946 |
| EcoRII | CCWGG | 1 cut(s) | 724 |
| EcoT14I | CCWWGG | 2 cut(s) | 716, 830 |
| ErhI | CCWWGG | 2 cut(s) | 716, 830 |
| FaeI | CATG | 3 cut(s) | 266, 434, 938 |
| FaqI | GGGAC | 2 cut(s) | 660, 698 |
| FatI | CATG | 3 cut(s) | 262, 430, 934 |
| Fnu4HI | GCNGC | 1 cut(s) | 394 |
| FokI | GGATG | 4 cut(s) | 240, 424, 708, 921 |
| Fsp4HI | GCNGC | 1 cut(s) | 394 |
| FspBI | CTAG | 1 cut(s) | 831 |
| GluI | GCNGC | 1 cut(s) | 394 |
| HapII | CCGG | 2 cut(s) | 300, 477 |
| HgaI | GACGC | 2 cut(s) | 12, 193 |
| Hin1II | CATG | 3 cut(s) | 266, 434, 938 |
| HinfI | GANTC | 7 cut(s) | 95, 113, 296, 305, 594, 665, 856 |
| HpaII | CCGG | 2 cut(s) | 300, 477 |
| HphI | GGTGA | 3 cut(s) | 41, 163, 659 |
| Hpy166II | GTNNAC | 3 cut(s) | 13, 400, 701 |
| Hpy188I | TCNGA | 6 cut(s) | 42, 100, 112, 415, 670, 750 |
| Hpy188III | TCNNGA | 1 cut(s) | 284 |
| Hpy8I | GTNNAC | 3 cut(s) | 13, 400, 701 |
| Hpy99I | CGWCG | 2 cut(s) | 187, 313 |
| HpyAV | CCTTC | 2 cut(s) | 415, 638 |
| HpyCH4III | ACNGT | 4 cut(s) | 197, 602, 625, 693 |
| HpyCH4IV | ACGT | 1 cut(s) | 462 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 239, 417, 486, 834 |
| HpyF3I | CTNAG | 1 cut(s) | 99 |
| HpySE526I | ACGT | 1 cut(s) | 462 |
| Hsp92II | CATG | 3 cut(s) | 266, 434, 938 |
| Kzo9I | GATC | 3 cut(s) | 31, 633, 781 |
| LmnI | GCTCC | 1 cut(s) | 914 |
| Lsp1109I | GCAGC | 1 cut(s) | 380 |
| LweI | GCATC | 4 cut(s) | 446, 499, 547, 730 |
| MaeI | CTAG | 1 cut(s) | 831 |
| MaeII | ACGT | 1 cut(s) | 462 |
| MalI | GATC | 3 cut(s) | 33, 635, 783 |
| MboI | GATC | 3 cut(s) | 31, 633, 781 |
| MboII | GAAGA | 4 cut(s) | 153, 195, 823, 865 |
| MflI | RGATCY | 1 cut(s) | 781 |
| MhlI | GDGCHC | 4 cut(s) | 476, 589, 703, 830 |
| MluCI | AATT | 2 cut(s) | 279, 890 |
| MlyI | GAGTC | 5 cut(s) | 104, 305, 588, 674, 850 |
| MseI | TTAA | 4 cut(s) | 36, 756, 792, 955 |
| MspI | CCGG | 2 cut(s) | 300, 477 |
| MspR9I | CCNGG | 2 cut(s) | 477, 726 |
| MvaI | CCWGG | 1 cut(s) | 726 |
| MvnI | CGCG | 1 cut(s) | 23 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 239, 417, 486, 834 |
| NciI | CCSGG | 1 cut(s) | 477 |
| NdeII | GATC | 3 cut(s) | 31, 633, 781 |
| NlaIII | CATG | 3 cut(s) | 266, 434, 938 |
| NlaIV | GGNNCC | 4 cut(s) | 199, 588, 827, 887 |
| NspI | RCATGY | 1 cut(s) | 434 |
| NspV | TTCGAA | 1 cut(s) | 382 |
| PaqCI | CACCTGC | 1 cut(s) | 873 |
| PfeI | GAWTC | 2 cut(s) | 113, 305 |
| PfoI | TCCNGGA | 1 cut(s) | 724 |
| PkrI | GCNGC | 1 cut(s) | 395 |
| PleI | GAGTC | 5 cut(s) | 103, 304, 588, 673, 850 |
| PpsI | GAGTC | 5 cut(s) | 103, 304, 588, 673, 850 |
| Ppu21I | YACGTR | 1 cut(s) | 463 |
| Psp6I | CCWGG | 1 cut(s) | 724 |
| PspGI | CCWGG | 1 cut(s) | 724 |
| PspN4I | GGNNCC | 4 cut(s) | 199, 588, 827, 887 |
| PspPI | GGNCC | 1 cut(s) | 924 |
| PstI | CTGCAG | 1 cut(s) | 491 |
| PsuI | RGATCY | 1 cut(s) | 781 |
| RsaI | GTAC | 1 cut(s) | 931 |
| RsaNI | GTAC | 1 cut(s) | 930 |
| SaqAI | TTAA | 4 cut(s) | 36, 756, 792, 955 |
| SatI | GCNGC | 1 cut(s) | 394 |
| Sau3AI | GATC | 3 cut(s) | 31, 633, 781 |
| Sau96I | GGNCC | 1 cut(s) | 924 |
| ScaI | AGTACT | 1 cut(s) | 931 |
| SchI | GAGTC | 5 cut(s) | 104, 305, 588, 674, 850 |
| ScrFI | CCNGG | 2 cut(s) | 477, 726 |
| SduI | GDGCHC | 4 cut(s) | 476, 589, 703, 830 |
| SetI | ASST | 8 cut(s) | 176, 465, 551, 574, 649, 836, 865, 887 |
| SfaNI | GCATC | 4 cut(s) | 446, 499, 547, 730 |
| SfcI | CTRYAG | 1 cut(s) | 487 |
| SfuI | TTCGAA | 1 cut(s) | 382 |
| SinI | GGWCC | 1 cut(s) | 924 |
| SmlI | CTYRAG | 2 cut(s) | 293, 858 |
| SmoI | CTYRAG | 2 cut(s) | 293, 858 |
| Sse9I | AATT | 2 cut(s) | 279, 890 |
| SsiI | CCGC | 4 cut(s) | 21, 66, 779, 823 |
| SspI | AATATT | 1 cut(s) | 842 |
| SspMI | CTAG | 1 cut(s) | 831 |
| StyD4I | CCNGG | 2 cut(s) | 475, 724 |
| StyI | CCWWGG | 2 cut(s) | 716, 830 |
| TaaI | ACNGT | 4 cut(s) | 197, 602, 625, 693 |
| TaiI | ACGT | 1 cut(s) | 465 |
| TaqI | TCGA | 3 cut(s) | 182, 382, 636 |
| TasI | AATT | 2 cut(s) | 279, 890 |
| TatI | WGTACW | 1 cut(s) | 929 |
| TfiI | GAWTC | 2 cut(s) | 113, 305 |
| Tru1I | TTAA | 4 cut(s) | 36, 756, 792, 955 |
| Tru9I | TTAA | 4 cut(s) | 36, 756, 792, 955 |
| TseI | GCWGC | 1 cut(s) | 393 |
| TspDTI | ATGAA | 4 cut(s) | 96, 201, 279, 374 |
| TspGWI | ACGGA | 1 cut(s) | 196 |
| VneI | GTGCAC | 1 cut(s) | 699 |
| VpaK11BI | GGWCC | 1 cut(s) | 924 |
| XceI | RCATGY | 1 cut(s) | 434 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 902 |
| XmaJI | CCTAGG | 1 cut(s) | 830 |
| XspI | CTAG | 1 cut(s) | 831 |
| ZrmI | AGTACT | 1 cut(s) | 931 |