RLG00000020271

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000271
Physical Location & Seq
Reverse (-)
63103262 .. 63104269
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020271

Sequence Viewer

Length: 873 bp
ATGGATGGGGCATCATCAAGCAGCTCTCATCCTAATATTTCTGATGATCATCATTTGGTTGATGATTTCTACTTCTCAGCTCTACATGATGACCAAGAAGTATTTCCCATCTCTGATGAGAAATATCCCCAAGAGTTGCAGCTTCAAGAGGCCTTCATGTCCTCTGCACTCTCTTTCAGAGTCCCAATTAGTAATGACCAAGTTGGAGTGGAAACACCCTTTGAAATACTAAAGGGACAATCTTCTGGCTCTGGAAGTTTCTGCATGATCTGTATGGATGTGAAGCCAAGTGGAGAAAGGTTCACAAACAATGGCTGCAACCACTCATTCTGCACCGTTTGCATTGGAAGGTACGTTGGGACTAAGATTCAGGAGAGCATTTCAATGGTGAAGTGTCCTGATGTGAAATGCAAAGGGGTATTGGAGCCCCAGTCATGTAGGTCATTTATTCCTCAGCAAGTGTTTGAAAGATGGGAAAGTGCTCTTTGTGAGTCCTTAGTTTTGGGTTCACTGAAATTTTACTGCCCTTTTAAGGACTGCTCAGCCATGCTTGTGGATGATGGAGGGGAAGTTGGTTGTGTGGCATGCACTCATGCAGGGATTGATTGTGGTGAGTTTCAGAGGTTGAGTAGGGATGAAAGAGGGATGGAAGATATCATGGTGATGGAACTTGATAAGAAGCAGCAATGGAGGAGATGCCCAAGGTGCAAGTTCTATGTGGAGAAGAATGCTGGCTGCTCACACATTACTTGCAGGCAGTATTCTGATTTGTTCTTTCTAATAGGTGTGGATTCGAATTTTGCTATGGCTGTGGATCCAATTGAAGTAATTGCCATGGATGTAGAGCGAACTGAGAACTTGAAAGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RING_UBOX

Protein Analysis

291

Amino Acids

32.46

Weight (kDa)

4.82

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14250
fragaria_vesca FvH4_1g09490 FvH4_1g09500 FvH4_1g09500 FvH4_1g09510 FvH4_3g00870 FvH4_3g03010 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g35700 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220
malus_domestica MD02G1102400.v1.1 MD02G1103700.v1.1 MD03G1111900.v1.1 MD09G1173800.v1.1 MD09G1230900.v1.1 MD09G1231400.v1.1 MD15G1224200.v1.1
prunus_persica Prupe.3G031900_v2.0.a1 Prupe.3G107200_v2.0.a1 Prupe.7G191200_v2.0.a1 Prupe.7G191800_v2.0.a1 Prupe.8G007700_v2.0.a1
pyrus_communis pycom02g08010 pycom02g08140 pycom09g14980 pycom09g15030 pycom15g19900
rosa_chinensis RchiOBHm_Chr2g0096041 RchiOBHm_Chr2g0096051 RchiOBHm_Chr2g0096061 RchiOBHm_Chr2g0096071 RchiOBHm_Chr2g0096081 RchiOBHm_Chr2g0096221 RchiOBHm_Chr2g0131511 RchiOBHm_Chr2g0147691 RchiOBHm_Chr5g0001281 RchiOBHm_Chr5g0001301
rosa_laevigata RLG00000016619 RLG00000016620 RLG00000016621 RLG00000016622 RLG00000019200 RLG00000020271 RLG00000025410 RLG00000030919 RLG00000031102 RLG00000031117 RLG00000031119 RLG00000031120
rosa_multiflora Rmu_co8160592.1_g000001 Rmu_sc0001706.1_g000008 Rmu_sc0002494.1_g000005 Rmu_sc0004189.1_g000040 Rmu_sc0005612.1_g000008 Rmu_sc0005612.1_g000011 Rmu_sc0005612.1_g000012 Rmu_sc0006926.1_g000015 Rmu_sc0013257.1_g000002 Rmu_sc0034363.1_g000001 Rmu_sc0034363.1_g000002
rosa_roxburghii Rroxscaffold_1G00072690 Rroxscaffold_1G00075020 Rroxscaffold_2G00145780 Rroxscaffold_2G00145790 Rroxscaffold_2G00145800 Rroxscaffold_2G00145810 Rroxscaffold_2G00145820 Rroxscaffold_2G00145830 Rroxscaffold_4G00300930 Rroxscaffold_6G00424750
rosa_rugosa Rorug02G0056000 Rorug02G0056100 Rorug02G0056200 Rorug02G0056300 Rorug02G0056500 Rorug02G0056700 Rorug02G0298000.1 Rorug02G0400900 Rorug02G0401000 Rorug02G0401100 Rorug04G0389500 Rorug04G0389600 Rorug04G0389700 Rorug04G0402300
rosa_samantha Rh2AG103800 Rh2AG458300 Rh2BG105400 Rh2BG105500 Rh2BG105600 Rh2BG105700 Rh2BG105900 Rh2BG357600 Rh2BG471000 Rh2DG106000 Rh2DG106100 Rh2DG106200 Rh2DG106300 Rh2DG106500 Rh2DG375400 Rh2DG480300 Rh5AG009500 Rh5AG009600 Rh5AG010000 Rh5AG028300 Rh5BG012400 Rh5BG012500 Rh5BG012700 Rh5BG028500
rosa_wichuraiana Rw2G007950 Rw2G007960 Rw2G007970 Rw2G028490 Rw5G000980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 809, 822
AcsI RAATTY 2 cut(s) 515, 796
AfaI GTAC 1 cut(s) 353
AfiI CCNNNNNNNGG 1 cut(s) 532
AgsI TTSAA 6 cut(s) 146, 224, 384, 467, 824, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 3 cut(s) 24, 80, 142
AluI AGCT 3 cut(s) 24, 80, 142
Alw21I GWGCWC 1 cut(s) 484
AlwI GGATC 2 cut(s) 809, 822
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 5 cut(s) 21, 139, 315, 682, 735
ApoI RAATTY 2 cut(s) 515, 796
Asp700I GAANNNNTTC 1 cut(s) 102
AsuHPI GGTGA 3 cut(s) 400, 623, 673
AsuII TTCGAA 1 cut(s) 794
BamHI GGATCC 1 cut(s) 814
BanII GRGCYC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 484
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 5 cut(s) 33, 151, 302, 694, 722
BccI CCATC 5 cut(s) 116, 465, 554, 640, 658
BclI TGATCA 1 cut(s) 46
BisI GCNGC 5 cut(s) 22, 140, 316, 683, 736
BlpI GCTNAGC 1 cut(s) 541
BlsI GCNGC 5 cut(s) 23, 141, 317, 684, 737
BmiI GGNNCC 2 cut(s) 426, 816
BmrI ACTGGG 1 cut(s) 424
BmsI GCATC 2 cut(s) 20, 686
BmuI ACTGGG 1 cut(s) 424
Bpu10I CCTNAGC 1 cut(s) 453
Bpu1102I GCTNAGC 1 cut(s) 541
Bpu14I TTCGAA 1 cut(s) 794
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 701, 834
BsaXI ACNNNNNCTCC 4 cut(s) 416, 446, 555, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 430
Bse3DI GCAATG 1 cut(s) 692
Bse8I GATNNNNATC 1 cut(s) 48
BseDI CCNNGG 2 cut(s) 701, 834
BseGI GGATG 7 cut(s) 10, 28, 283, 562, 640, 651, 844
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 532
BseMI GCAATG 1 cut(s) 692
BseMII CTCAG 4 cut(s) 90, 467, 555, 843
BseNI ACTGG 1 cut(s) 430
BseRI GAGGAG 1 cut(s) 706
BseXI GCAGC 5 cut(s) 33, 151, 302, 694, 722
BsgI GTGCAG 2 cut(s) 150, 316
BshFI GGCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 484
BslFI GGGAC 3 cut(s) 167, 249, 373
BslI CCNNNNNNNGG 1 cut(s) 532
BsmFI GGGAC 3 cut(s) 167, 249, 373
BsmI GAATGC 1 cut(s) 733
BsnI GGCC 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 794
Bsp1286I GDGCHC 2 cut(s) 429, 484
Bsp143I GATC 3 cut(s) 46, 267, 814
Bsp1720I GCTNAGC 1 cut(s) 541
Bsp19I CCATGG 1 cut(s) 834
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 4 cut(s) 89, 466, 554, 844
BspLI GGNNCC 2 cut(s) 426, 816
BspPI GGATC 2 cut(s) 809, 822
BspT104I TTCGAA 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 692
BsrI ACTGG 1 cut(s) 430
BssECI CCNNGG 2 cut(s) 701, 834
BssMI GATC 3 cut(s) 46, 267, 814
BssT1I CCWWGG 2 cut(s) 701, 834
Bst4CI ACNGT 1 cut(s) 337
BstAPI GCANNNNNTGC 1 cut(s) 339
BstBI TTCGAA 1 cut(s) 794
BstC8I GCNNGC 3 cut(s) 586, 733, 755
BstDEI CTNAG 6 cut(s) 76, 363, 453, 496, 541, 852
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 7 cut(s) 10, 28, 283, 562, 640, 651, 844
BstKTI GATC 3 cut(s) 49, 270, 817
BstMBI GATC 3 cut(s) 46, 267, 814
BstMWI GCNNNNNNNGC 2 cut(s) 339, 705
BstNSI RCATGY 1 cut(s) 588
BstV1I GCAGC 5 cut(s) 33, 151, 302, 694, 722
BstX2I RGATCY 1 cut(s) 814
BstXI CCANNNNNNTGG 1 cut(s) 553
BstYI RGATCY 1 cut(s) 814
BsuRI GGCC 1 cut(s) 152
BtgI CCRYGG 1 cut(s) 834
BtsCI GGATG 7 cut(s) 10, 28, 283, 562, 640, 651, 844
BtsIMutI CAGTG 1 cut(s) 509
Cac8I GCNNGC 3 cut(s) 586, 733, 755
Csp6I GTAC 1 cut(s) 352
CviAII CATG 9 cut(s) 86, 157, 265, 435, 547, 585, 593, 658, 835
CviQI GTAC 1 cut(s) 352
DdeI CTNAG 6 cut(s) 76, 363, 453, 496, 541, 852
DpnI GATC 3 cut(s) 48, 269, 816
DpnII GATC 3 cut(s) 46, 267, 814
Eco130I CCWWGG 2 cut(s) 701, 834
Eco147I AGGCCT 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 429
Eco32I GATATC 1 cut(s) 655
EcoRV GATATC 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 701, 834
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 2 cut(s) 701, 834
FaeI CATG 9 cut(s) 89, 160, 268, 438, 550, 588, 596, 661, 838
FaqI GGGAC 3 cut(s) 167, 249, 373
FatI CATG 9 cut(s) 85, 156, 264, 434, 546, 584, 592, 657, 834
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 5 cut(s) 22, 140, 316, 683, 736
FokI GGATG 7 cut(s) 15, 17, 290, 569, 647, 658, 851
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 22, 140, 316, 683, 736
GluI GCNGC 5 cut(s) 22, 140, 316, 683, 736
HaeIII GGCC 1 cut(s) 152
Hin1II CATG 9 cut(s) 89, 160, 268, 438, 550, 588, 596, 661, 838
HinfI GANTC 4 cut(s) 180, 367, 491, 791
HphI GGTGA 3 cut(s) 400, 623, 673
Hpy166II GTNNAC 2 cut(s) 303, 509
Hpy188I TCNGA 5 cut(s) 43, 115, 179, 621, 766
Hpy188III TCNNGA 4 cut(s) 146, 252, 371, 398
Hpy8I GTNNAC 2 cut(s) 303, 509
HpyAV CCTTC 2 cut(s) 163, 342
HpyCH4III ACNGT 1 cut(s) 337
HpyCH4IV ACGT 1 cut(s) 354
HpyF10VI GCNNNNNNNGC 2 cut(s) 339, 705
HpyF3I CTNAG 6 cut(s) 76, 363, 453, 496, 541, 852
HpySE526I ACGT 1 cut(s) 354
Hsp92II CATG 9 cut(s) 89, 160, 268, 438, 550, 588, 596, 661, 838
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 3 cut(s) 46, 267, 814
LmnI GCTCC 1 cut(s) 424
LpnPI CCDG 8 cut(s) 231, 237, 356, 411, 443, 582, 717, 739
Lsp1109I GCAGC 5 cut(s) 33, 151, 302, 694, 722
LweI GCATC 2 cut(s) 20, 686
MaeII ACGT 1 cut(s) 354
MalI GATC 3 cut(s) 48, 269, 816
MboI GATC 3 cut(s) 46, 267, 814
MboII GAAGA 3 cut(s) 234, 662, 736
MfeI CAATTG 1 cut(s) 819
MflI RGATCY 1 cut(s) 814
MhlI GDGCHC 2 cut(s) 429, 484
MluCI AATT 5 cut(s) 186, 515, 796, 819, 828
MlyI GAGTC 2 cut(s) 189, 500
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 7 cut(s) 142, 172, 462, 557, 615, 635, 684
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 3 cut(s) 383, 551, 662
MunI CAATTG 1 cut(s) 819
Mva1269I GAATGC 1 cut(s) 733
MwoI GCNNNNNNNGC 2 cut(s) 339, 705
NcoI CCATGG 1 cut(s) 834
NdeII GATC 3 cut(s) 46, 267, 814
NlaIII CATG 9 cut(s) 89, 160, 268, 438, 550, 588, 596, 661, 838
NlaIV GGNNCC 2 cut(s) 426, 816
NspI RCATGY 1 cut(s) 588
NspV TTCGAA 1 cut(s) 794
PaeI GCATGC 1 cut(s) 588
PceI AGGCCT 1 cut(s) 152
PctI GAATGC 1 cut(s) 733
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 2 cut(s) 367, 791
PkrI GCNGC 5 cut(s) 23, 141, 317, 684, 737
PleI GAGTC 2 cut(s) 188, 499
PpsI GAGTC 2 cut(s) 188, 499
PspN4I GGNNCC 2 cut(s) 426, 816
PsuI RGATCY 1 cut(s) 814
RsaI GTAC 1 cut(s) 353
RsaNI GTAC 1 cut(s) 352
RseI CAYNNNNRTG 3 cut(s) 383, 551, 662
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 5 cut(s) 22, 140, 316, 683, 736
Sau3AI GATC 3 cut(s) 46, 267, 814
SchI GAGTC 2 cut(s) 189, 500
SduI GDGCHC 2 cut(s) 429, 484
SfaNI GCATC 2 cut(s) 20, 686
SfuI TTCGAA 1 cut(s) 794
SmiMI CAYNNNNRTG 3 cut(s) 383, 551, 662
SphI GCATGC 1 cut(s) 588
Sse9I AATT 5 cut(s) 186, 515, 796, 819, 828
SseBI AGGCCT 1 cut(s) 152
SspI AATATT 1 cut(s) 37
StuI AGGCCT 1 cut(s) 152
StyI CCWWGG 2 cut(s) 701, 834
TaaI ACNGT 1 cut(s) 337
TaiI ACGT 1 cut(s) 357
TaqI TCGA 1 cut(s) 794
TasI AATT 5 cut(s) 186, 515, 796, 819, 828
TfiI GAWTC 2 cut(s) 367, 791
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 1 cut(s) 516
TseI GCWGC 5 cut(s) 21, 139, 315, 682, 735
TspDTI ATGAA 2 cut(s) 145, 651
TspRI CASTG 1 cut(s) 516
XapI RAATTY 2 cut(s) 515, 796
XceI RCATGY 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.