FvH4_6g35700

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000271
Physical Location & Seq
Reverse (-)
28175937 .. 28176931
995 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g35700.t1

Sequence Viewer

Length: 867 bp
ATGGATGGGGCATCATCAAGCATCTCTCATCCTAATACTTCTGATGATCATCATTTGGTTGATGATTTCTACTTCTCGGCTCTCTATGATGACGAAGAAGTATTTCCCATCTGTGATGAGAAATATGCCCAGGAGTTGCAGCTTCAAGAGGCTCTCATGTCATCTGCAATCTCTTCCTCCAAAGTCCCAATTAGTATTGACCAATTTGAAGTAAAAACACCCTTTGAAATACTGAGGGGACAATCTTCTGGCTCTGGAAGCTTCTGCATGATCTGTATGGATGTGAAACCAAGTGGAGAAATGTTCACAAACAATAGCTGCAACCACTCATTCTGCACTGATTGCATTGGAAGGTATGTTGCGACTAAGATTCAGGAGAACATTTCAATGGTTAAGTGTCCTGATGTGAAATGCAAAGAGGTATTGGAGCCACAGTCATGCAGGTCATTTATTCCTCAGCAAGTGTTTGAAAGATGGGAAAGTGCGCTTTGTGAATCCTTGTTTCTGGCTTCAGACAGATTTTACTGCCCTTTTAAGGACTGCTCAATGATGCTTGTGGATGATGGAGGGGAGGTTGTGACTGTTTCGGAGTGCCCCAACTGTCGCAGACTCTTTTGTGCTCAATGCAGGGTTGTGTGGCATGCCGGGATTGATTGTAGCGAGTTTCAGCAGTTGAGTAAGGATGAAAGAGGGAGGGAAGACATCATGGTGATGGAGCTTGCTCATCAGAAGCAATGGAGGAGATGCCCAAGGTGCAAGTTCTATGTGGAAAAGACTGCTGGTTGCTCACACATTACTTGCAGGTGTGGATTCGAATTTTGCTATGGCTGTGGATCCAATTATGGGCACTGTAATGGATGTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RING_UBOX

Protein Analysis

289

Amino Acids

32.62

Weight (kDa)

5.0

Isoelectric Point (pI)

54.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IBR PF01485 158 - 219 3.4e-10 IBR domain, a half RING-finger domain
IBR PF01485 240 - 283 7.4e-07 IBR domain, a half RING-finger domain
IBR_1 PF22191 248 - 283 7.7e-06 IBR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14250
fragaria_vesca FvH4_1g09490 FvH4_1g09500 FvH4_1g09500 FvH4_1g09510 FvH4_3g00870 FvH4_3g03010 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g35700 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220
malus_domestica MD02G1102400.v1.1 MD02G1103700.v1.1 MD03G1111900.v1.1 MD09G1173800.v1.1 MD09G1230900.v1.1 MD09G1231400.v1.1 MD15G1224200.v1.1
prunus_persica Prupe.3G031900_v2.0.a1 Prupe.3G107200_v2.0.a1 Prupe.7G191200_v2.0.a1 Prupe.7G191800_v2.0.a1 Prupe.8G007700_v2.0.a1
pyrus_communis pycom02g08010 pycom02g08140 pycom09g14980 pycom09g15030 pycom15g19900
rosa_chinensis RchiOBHm_Chr2g0096041 RchiOBHm_Chr2g0096051 RchiOBHm_Chr2g0096061 RchiOBHm_Chr2g0096071 RchiOBHm_Chr2g0096081 RchiOBHm_Chr2g0096221 RchiOBHm_Chr2g0131511 RchiOBHm_Chr2g0147691 RchiOBHm_Chr5g0001281 RchiOBHm_Chr5g0001301
rosa_laevigata RLG00000016619 RLG00000016620 RLG00000016621 RLG00000016622 RLG00000019200 RLG00000020271 RLG00000025410 RLG00000030919 RLG00000031102 RLG00000031117 RLG00000031119 RLG00000031120
rosa_multiflora Rmu_co8160592.1_g000001 Rmu_sc0001706.1_g000008 Rmu_sc0002494.1_g000005 Rmu_sc0004189.1_g000040 Rmu_sc0005612.1_g000008 Rmu_sc0005612.1_g000011 Rmu_sc0005612.1_g000012 Rmu_sc0006926.1_g000015 Rmu_sc0013257.1_g000002 Rmu_sc0034363.1_g000001 Rmu_sc0034363.1_g000002
rosa_roxburghii Rroxscaffold_1G00072690 Rroxscaffold_1G00075020 Rroxscaffold_2G00145780 Rroxscaffold_2G00145790 Rroxscaffold_2G00145800 Rroxscaffold_2G00145810 Rroxscaffold_2G00145820 Rroxscaffold_2G00145830 Rroxscaffold_4G00300930 Rroxscaffold_6G00424750
rosa_rugosa Rorug02G0056000 Rorug02G0056100 Rorug02G0056200 Rorug02G0056300 Rorug02G0056500 Rorug02G0056700 Rorug02G0298000.1 Rorug02G0400900 Rorug02G0401000 Rorug02G0401100 Rorug04G0389500 Rorug04G0389600 Rorug04G0389700 Rorug04G0402300
rosa_samantha Rh2AG103800 Rh2AG458300 Rh2BG105400 Rh2BG105500 Rh2BG105600 Rh2BG105700 Rh2BG105900 Rh2BG357600 Rh2BG471000 Rh2DG106000 Rh2DG106100 Rh2DG106200 Rh2DG106300 Rh2DG106500 Rh2DG375400 Rh2DG480300 Rh5AG009500 Rh5AG009600 Rh5AG010000 Rh5AG028300 Rh5BG012400 Rh5BG012500 Rh5BG012700 Rh5BG028500
rosa_wichuraiana Rw2G007950 Rw2G007960 Rw2G007970 Rw2G028490 Rw5G000980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 792
Acc36I ACCTGC 2 cut(s) 432, 792
AclWI GGATC 2 cut(s) 828, 841
AcsI RAATTY 1 cut(s) 815
AcuI CTGAAG 1 cut(s) 495
AfiI CCNNNNNNNGG 2 cut(s) 535, 843
AgsI TTSAA 5 cut(s) 146, 209, 227, 387, 470
AjnI CCWGG 1 cut(s) 129
AluBI AGCT 4 cut(s) 142, 261, 318, 718
AluI AGCT 4 cut(s) 142, 261, 318, 718
Alw21I GWGCWC 1 cut(s) 622
AlwI GGATC 2 cut(s) 828, 841
ApeKI GCWGC 2 cut(s) 139, 318
ApoI RAATTY 1 cut(s) 815
Asp700I GAANNNNTTC 1 cut(s) 102
AspLEI GCGC 1 cut(s) 487
AsuC2I CCSGG 1 cut(s) 646
AsuHPI GGTGA 1 cut(s) 721
AsuII TTCGAA 1 cut(s) 813
BaeGI GKGCMC 2 cut(s) 596, 849
BamHI GGATCC 1 cut(s) 833
BbsI GAAGAC 1 cut(s) 705
Bbv12I GWGCWC 1 cut(s) 622
BbvCI CCTCAGC 1 cut(s) 456
BbvI GCAGC 2 cut(s) 151, 305
BccI CCATC 4 cut(s) 116, 468, 557, 706
BciT130I CCWGG 1 cut(s) 131
BclI TGATCA 1 cut(s) 46
BcnI CCSGG 1 cut(s) 646
BfuAI ACCTGC 2 cut(s) 432, 792
BisI GCNGC 2 cut(s) 140, 319
BlsI GCNGC 2 cut(s) 141, 320
Bme1390I CCNGG 2 cut(s) 131, 646
BmiI GGNNCC 2 cut(s) 429, 835
BmrFI CCNGG 2 cut(s) 131, 646
BmsI GCATC 4 cut(s) 20, 30, 540, 734
BpiI GAAGAC 1 cut(s) 705
Bpu10I CCTNAGC 1 cut(s) 456
Bpu14I TTCGAA 1 cut(s) 813
BpuMI CCSGG 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 2 cut(s) 129, 749
BsaXI ACNNNNNCTCC 4 cut(s) 419, 449, 558, 588
Bsc4I CCNNNNNNNGG 2 cut(s) 535, 843
Bse3DI GCAATG 1 cut(s) 740
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 131
BseDI CCNNGG 2 cut(s) 129, 749
BseGI GGATG 6 cut(s) 10, 28, 286, 565, 688, 863
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 2 cut(s) 535, 843
BseMI GCAATG 1 cut(s) 740
BseMII CTCAG 2 cut(s) 224, 470
BseRI GAGGAG 1 cut(s) 754
BseSI GKGCMC 2 cut(s) 596, 849
BseXI GCAGC 2 cut(s) 151, 305
BsgI GTGCAG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 1 cut(s) 645
BslFI GGGAC 2 cut(s) 170, 252
BslI CCNNNNNNNGG 2 cut(s) 535, 843
BsmFI GGGAC 2 cut(s) 170, 252
Bsp119I TTCGAA 1 cut(s) 813
Bsp1286I GDGCHC 3 cut(s) 596, 622, 849
Bsp143I GATC 3 cut(s) 46, 270, 833
BspCNI CTCAG 2 cut(s) 225, 469
BspLI GGNNCC 2 cut(s) 429, 835
BspMI ACCTGC 2 cut(s) 432, 792
BspPI GGATC 2 cut(s) 828, 841
BspT104I TTCGAA 1 cut(s) 813
BsrDI GCAATG 1 cut(s) 740
BssECI CCNNGG 2 cut(s) 129, 749
BssMI GATC 3 cut(s) 46, 270, 833
BssT1I CCWWGG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 131
Bst4CI ACNGT 4 cut(s) 435, 583, 602, 851
Bst6I CTCTTC 1 cut(s) 178
BstAPI GCANNNNNTGC 1 cut(s) 342
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 2 cut(s) 642, 720
BstDEI CTNAG 3 cut(s) 233, 366, 456
BstF5I GGATG 6 cut(s) 10, 28, 286, 565, 688, 863
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 3 cut(s) 49, 273, 836
BstMBI GATC 3 cut(s) 46, 270, 833
BstMWI GCNNNNNNNGC 3 cut(s) 258, 342, 753
BstNI CCWGG 1 cut(s) 131
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 2 cut(s) 129, 644
BstSLI GKGCMC 2 cut(s) 596, 849
BstV1I GCAGC 2 cut(s) 151, 305
BstV2I GAAGAC 1 cut(s) 705
BstX2I RGATCY 1 cut(s) 833
BstYI RGATCY 1 cut(s) 833
BtsCI GGATG 6 cut(s) 10, 28, 286, 565, 688, 863
BtsIMutI CAGTG 2 cut(s) 336, 847
BveI ACCTGC 2 cut(s) 432, 792
Cac8I GCNNGC 2 cut(s) 642, 720
CfoI GCGC 1 cut(s) 487
CviAII CATG 5 cut(s) 157, 268, 438, 641, 706
DdeI CTNAG 3 cut(s) 233, 366, 456
DpnI GATC 3 cut(s) 48, 272, 835
DpnII GATC 3 cut(s) 46, 270, 833
Eam1104I CTCTTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 178
Eco130I CCWWGG 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 495
EcoRII CCWGG 1 cut(s) 129
EcoT14I CCWWGG 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 749
FaeI CATG 5 cut(s) 160, 271, 441, 644, 709
FaqI GGGAC 2 cut(s) 170, 252
FatI CATG 5 cut(s) 156, 267, 437, 640, 705
FbaI TGATCA 1 cut(s) 46
Fnu4HI GCNGC 2 cut(s) 140, 319
FokI GGATG 5 cut(s) 15, 17, 293, 572, 695
Fsp4HI GCNGC 2 cut(s) 140, 319
GlaI GCGC 1 cut(s) 486
GluI GCNGC 2 cut(s) 140, 319
HapII CCGG 1 cut(s) 645
HhaI GCGC 1 cut(s) 487
Hin1II CATG 5 cut(s) 160, 271, 441, 644, 709
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HindIII AAGCTT 1 cut(s) 259
HinfI GANTC 4 cut(s) 370, 494, 609, 810
HpaII CCGG 1 cut(s) 645
HphI GGTGA 1 cut(s) 721
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 4 cut(s) 43, 514, 589, 729
Hpy188III TCNNGA 4 cut(s) 146, 255, 374, 401
Hpy8I GTNNAC 1 cut(s) 306
HpyAV CCTTC 1 cut(s) 345
HpyCH4III ACNGT 4 cut(s) 435, 583, 602, 851
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 342, 753
HpyF3I CTNAG 3 cut(s) 233, 366, 456
Hsp92II CATG 5 cut(s) 160, 271, 441, 644, 709
HspAI GCGC 1 cut(s) 485
Ksp22I TGATCA 1 cut(s) 46
Kzo9I GATC 3 cut(s) 46, 270, 833
LmnI GCTCC 2 cut(s) 427, 715
Lsp1109I GCAGC 2 cut(s) 151, 305
LweI GCATC 4 cut(s) 20, 30, 540, 734
MaeIII GTNAC 1 cut(s) 577
MalI GATC 3 cut(s) 48, 272, 835
MboI GATC 3 cut(s) 46, 270, 833
MboII GAAGA 4 cut(s) 107, 165, 237, 710
MflI RGATCY 1 cut(s) 833
MhlI GDGCHC 3 cut(s) 596, 622, 849
MluCI AATT 5 cut(s) 189, 203, 815, 838, 862
MlyI GAGTC 1 cut(s) 603
MroXI GAANNNNTTC 1 cut(s) 102
MseI TTAA 3 cut(s) 393, 534, 865
MslI CAYNNNNRTG 5 cut(s) 386, 436, 707, 710, 852
MspI CCGG 1 cut(s) 645
MspR9I CCNGG 2 cut(s) 131, 646
MvaI CCWGG 1 cut(s) 131
MwoI GCNNNNNNNGC 3 cut(s) 258, 342, 753
NciI CCSGG 1 cut(s) 646
NdeII GATC 3 cut(s) 46, 270, 833
NlaIII CATG 5 cut(s) 160, 271, 441, 644, 709
NlaIV GGNNCC 2 cut(s) 429, 835
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 1 cut(s) 577
NspI RCATGY 1 cut(s) 644
NspV TTCGAA 1 cut(s) 813
PaeI GCATGC 1 cut(s) 644
PaqCI CACCTGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 102
PfeI GAWTC 3 cut(s) 370, 494, 810
PkrI GCNGC 2 cut(s) 141, 320
PleI GAGTC 1 cut(s) 603
PpsI GAGTC 1 cut(s) 603
Psp6I CCWGG 1 cut(s) 129
PspGI CCWGG 1 cut(s) 129
PspN4I GGNNCC 2 cut(s) 429, 835
PsuI RGATCY 1 cut(s) 833
RseI CAYNNNNRTG 5 cut(s) 386, 436, 707, 710, 852
SaqAI TTAA 3 cut(s) 393, 534, 865
SatI GCNGC 2 cut(s) 140, 319
Sau3AI GATC 3 cut(s) 46, 270, 833
SchI GAGTC 1 cut(s) 603
ScrFI CCNGG 2 cut(s) 131, 646
SduI GDGCHC 3 cut(s) 596, 622, 849
SfaNI GCATC 4 cut(s) 20, 30, 540, 734
SfuI TTCGAA 1 cut(s) 813
SmiMI CAYNNNNRTG 5 cut(s) 386, 436, 707, 710, 852
SphI GCATGC 1 cut(s) 644
Sse9I AATT 5 cut(s) 189, 203, 815, 838, 862
StyD4I CCNGG 2 cut(s) 129, 644
StyI CCWWGG 1 cut(s) 749
TaaI ACNGT 4 cut(s) 435, 583, 602, 851
TaqI TCGA 1 cut(s) 813
TasI AATT 5 cut(s) 189, 203, 815, 838, 862
TfiI GAWTC 3 cut(s) 370, 494, 810
Tru1I TTAA 3 cut(s) 393, 534, 865
Tru9I TTAA 3 cut(s) 393, 534, 865
TscAI CASTG 2 cut(s) 343, 854
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 2 cut(s) 139, 318
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 1 cut(s) 699
TspRI CASTG 2 cut(s) 343, 854
XapI RAATTY 1 cut(s) 815
XceI RCATGY 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.