Rorug02G0400900

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000271
Physical Location & Seq
Reverse (-)
51212643 .. 51214944
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0400900.1

Sequence Viewer

Length: 948 bp
ATGGACTCCATTGATGTGCTCATGCAAGTCCCAACATCGATAAGCGCTTATTTAGAACAAGAACTCGATAAGCGCTTCAACGTCCTCAAGCTCTGGACTGTCCCTAACAAAACCCAGTTCATCAAGGACAACTCCAGTTCCATCCGAGCCATCGTCGGCCACGGCGGCGCTGGAGCTGATGCCCATCTCATCGACAGTCTGCCCAACTTGGAGATCATCTCCAGCTTCAGCGTTGGGACGGACAAGATTGATTTGAAGAAATGCAAGGAAAAGGGTATTCGGGTCACCAACACACCCGACGTGTTGACCGATGACGTGGCCGACATCGCCATCGGGTTGGCGCTGGCGGTCATGCGGAGGCTGTGTGAGAGTGACCGCTATGTCAGGAGTGGGCAGTGGAAGAAGGGTAACTACAAGTTGACCACAAAGTTCACCGGAAAAACAGTTGGTATCATCGGTTTGGGAAGAATCGGCAAAGCAGTTGCCAAGAGAGCTGAGGCTTTTAGCTGCCCCATTGCCTACTTCTCCAGAACAGAAAAACCAGAATTGAAGTACAAGTATTATCCTACTGTTGTGGAATTGGCCTCCAACTGTGATGTTCTAGTTGTTGCGTGCCCACTCACTGAAGAAACCCGCCACATTATCAATCGTGAAGTCATTGATGCATTGGGGCCAAAGGGTGTTCTCATTAACATTGGGAGGGGTCATCATGTTGATGAGCCTGAGTTAGTATCTGCACTGCTAGAAGGCCGATTAGGTGGAGCCGGCCTTGATGTGTATCAAAATGAACCAGAAGTACCAGAGCAGCTGTTTGGTCTTGAAAATGTGGTGCTCTTGCCTCATGCAGGAAGTGGTACTACTGAAACTCGCAATGCAATGGCTGACCTTGTTATTGGGAATCTTGAGGCTCACTTCTTGAACAAACCATTGCTGACCCCTGTGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IBR zf-RING_UBOX

Protein Analysis

315

Amino Acids

34.36

Weight (kDa)

6.2

Isoelectric Point (pI)

29.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
2-Hacid_dh PF00389 18 - 314 5.5e-24 D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domain
2-Hacid_dh_C PF02826 111 - 283 6.2e-55 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000271)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G14250
fragaria_vesca FvH4_1g09490 FvH4_1g09500 FvH4_1g09500 FvH4_1g09510 FvH4_3g00870 FvH4_3g03010 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g27960 FvH4_6g35700 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220 FvH4_7g07220
malus_domestica MD02G1102400.v1.1 MD02G1103700.v1.1 MD03G1111900.v1.1 MD09G1173800.v1.1 MD09G1230900.v1.1 MD09G1231400.v1.1 MD15G1224200.v1.1
prunus_persica Prupe.3G031900_v2.0.a1 Prupe.3G107200_v2.0.a1 Prupe.7G191200_v2.0.a1 Prupe.7G191800_v2.0.a1 Prupe.8G007700_v2.0.a1
pyrus_communis pycom02g08010 pycom02g08140 pycom09g14980 pycom09g15030 pycom15g19900
rosa_chinensis RchiOBHm_Chr2g0096041 RchiOBHm_Chr2g0096051 RchiOBHm_Chr2g0096061 RchiOBHm_Chr2g0096071 RchiOBHm_Chr2g0096081 RchiOBHm_Chr2g0096221 RchiOBHm_Chr2g0131511 RchiOBHm_Chr2g0147691 RchiOBHm_Chr5g0001281 RchiOBHm_Chr5g0001301
rosa_laevigata RLG00000016619 RLG00000016620 RLG00000016621 RLG00000016622 RLG00000019200 RLG00000020271 RLG00000025410 RLG00000030919 RLG00000031102 RLG00000031117 RLG00000031119 RLG00000031120
rosa_multiflora Rmu_co8160592.1_g000001 Rmu_sc0001706.1_g000008 Rmu_sc0002494.1_g000005 Rmu_sc0004189.1_g000040 Rmu_sc0005612.1_g000008 Rmu_sc0005612.1_g000011 Rmu_sc0005612.1_g000012 Rmu_sc0006926.1_g000015 Rmu_sc0013257.1_g000002 Rmu_sc0034363.1_g000001 Rmu_sc0034363.1_g000002
rosa_roxburghii Rroxscaffold_1G00072690 Rroxscaffold_1G00075020 Rroxscaffold_2G00145780 Rroxscaffold_2G00145790 Rroxscaffold_2G00145800 Rroxscaffold_2G00145810 Rroxscaffold_2G00145820 Rroxscaffold_2G00145830 Rroxscaffold_4G00300930 Rroxscaffold_6G00424750
rosa_rugosa Rorug02G0056000 Rorug02G0056100 Rorug02G0056200 Rorug02G0056300 Rorug02G0056500 Rorug02G0056700 Rorug02G0298000.1 Rorug02G0400900 Rorug02G0401000 Rorug02G0401100 Rorug04G0389500 Rorug04G0389600 Rorug04G0389700 Rorug04G0402300
rosa_samantha Rh2AG103800 Rh2AG458300 Rh2BG105400 Rh2BG105500 Rh2BG105600 Rh2BG105700 Rh2BG105900 Rh2BG357600 Rh2BG471000 Rh2DG106000 Rh2DG106100 Rh2DG106200 Rh2DG106300 Rh2DG106500 Rh2DG375400 Rh2DG480300 Rh5AG009500 Rh5AG009600 Rh5AG010000 Rh5AG028300 Rh5BG012400 Rh5BG012500 Rh5BG012700 Rh5BG028500
rosa_wichuraiana Rw2G007950 Rw2G007960 Rw2G007970 Rw2G028490 Rw5G000980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 380
AciI CCGC 5 cut(s) 165, 347, 355, 376, 634
AcoI YGGCCR 2 cut(s) 157, 318
AcuI CTGAAG 2 cut(s) 211, 645
AfaI GTAC 3 cut(s) 554, 798, 856
AfeI AGCGCT 2 cut(s) 46, 74
AfiI CCNNNNNNNGG 1 cut(s) 845
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 5 cut(s) 79, 256, 550, 821, 919
AjiI CACGTC 2 cut(s) 301, 316
AluBI AGCT 6 cut(s) 91, 176, 225, 494, 507, 808
AluI AGCT 6 cut(s) 91, 176, 225, 494, 507, 808
Alw21I GWGCWC 2 cut(s) 21, 834
Aor51HI AGCGCT 2 cut(s) 46, 74
AoxI GGCC 6 cut(s) 157, 318, 582, 671, 748, 766
ApeKI GCWGC 2 cut(s) 507, 805
AspLEI GCGC 4 cut(s) 47, 75, 170, 343
AspS9I GGNCC 1 cut(s) 671
AsuHPI GGTGA 2 cut(s) 277, 424
BaeGI GKGCMC 1 cut(s) 617
BarI GAAGNNNNNNTAC 4 cut(s) 395, 427, 841, 873
Bbv12I GWGCWC 2 cut(s) 21, 834
BbvCI CCTCAGC 1 cut(s) 495
BbvI GCAGC 2 cut(s) 494, 817
BccI CCATC 4 cut(s) 149, 158, 192, 338
BceAI ACGGC 1 cut(s) 178
BfaI CTAG 2 cut(s) 602, 743
BfoI RGCGCY 4 cut(s) 48, 76, 171, 344
BglI GCCNNNNNGGC 1 cut(s) 165
BisI GCNGC 3 cut(s) 166, 508, 806
BlsI GCNGC 3 cut(s) 167, 509, 807
BmgBI CACGTC 2 cut(s) 301, 316
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 2 cut(s) 672, 763
BmrI ACTGGG 1 cut(s) 109
BmsI GCATC 2 cut(s) 169, 652
BmuI ACTGGG 1 cut(s) 109
BplI GAGNNNNNCTC 2 cut(s) 203, 235
BpmI CTGGAG 4 cut(s) 118, 192, 205, 511
Bpu10I CCTNAGC 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 71, 923
Bsa29I ATCGAT 1 cut(s) 38
BsaBI GATNNNNATC 2 cut(s) 183, 777
BsaJI CCNNGG 1 cut(s) 160
BsaWI WCCGGW 1 cut(s) 434
BsaXI ACNNNNNCTCC 2 cut(s) 349, 379
Bsc4I CCNNNNNNNGG 1 cut(s) 845
Bse118I RCCGGY 1 cut(s) 764
Bse1I ACTGG 2 cut(s) 115, 135
Bse3DI GCAATG 4 cut(s) 513, 877, 882, 926
Bse8I GATNNNNATC 2 cut(s) 183, 777
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 160
BseGI GGATG 1 cut(s) 141
BseJI GATNNNNATC 2 cut(s) 183, 777
BseLI CCNNNNNNNGG 1 cut(s) 845
BseMI GCAATG 4 cut(s) 513, 877, 882, 926
BseMII CTCAG 2 cut(s) 486, 714
BseNI ACTGG 2 cut(s) 115, 135
BseSI GKGCMC 1 cut(s) 617
BseXI GCAGC 2 cut(s) 494, 817
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 6 cut(s) 159, 320, 584, 673, 750, 768
BshVI ATCGAT 1 cut(s) 38
BsiHKAI GWGCWC 2 cut(s) 21, 834
BsiSI CCGG 2 cut(s) 435, 765
BslFI GGGAC 3 cut(s) 14, 86, 250
BslI CCNNNNNNNGG 1 cut(s) 845
BsmFI GGGAC 3 cut(s) 14, 86, 250
BsnI GGCC 6 cut(s) 159, 320, 584, 673, 750, 768
Bsp1286I GDGCHC 3 cut(s) 21, 617, 834
Bsp143I GATC 1 cut(s) 213
BspACI CCGC 5 cut(s) 165, 347, 355, 376, 634
BspANI GGCC 6 cut(s) 159, 320, 584, 673, 750, 768
BspCNI CTCAG 2 cut(s) 487, 715
BspDI ATCGAT 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 672, 763
BsrDI GCAATG 4 cut(s) 513, 877, 882, 926
BsrFI RCCGGY 1 cut(s) 764
BsrI ACTGG 2 cut(s) 115, 135
BssAI RCCGGY 1 cut(s) 764
BssECI CCNNGG 1 cut(s) 160
BssMI GATC 1 cut(s) 213
Bst4CI ACNGT 5 cut(s) 100, 197, 445, 571, 593
BstC8I GCNNGC 3 cut(s) 345, 613, 766
BstDEI CTNAG 2 cut(s) 495, 723
BstDSI CCRYGG 1 cut(s) 160
BstEII GGTNACC 1 cut(s) 283
BstENI CCTNNNNNAGG 1 cut(s) 843
BstF5I GGATG 1 cut(s) 141
BstH2I RGCGCY 4 cut(s) 48, 76, 171, 344
BstHHI GCGC 4 cut(s) 47, 75, 170, 343
BstKTI GATC 1 cut(s) 216
BstMBI GATC 1 cut(s) 213
BstMWI GCNNNNNNNGC 3 cut(s) 165, 326, 491
BstPI GGTNACC 1 cut(s) 283
BstSLI GKGCMC 1 cut(s) 617
BstV1I GCAGC 2 cut(s) 494, 817
BstXI CCANNNNNNTGG 1 cut(s) 337
Bsu15I ATCGAT 1 cut(s) 38
BsuRI GGCC 6 cut(s) 159, 320, 584, 673, 750, 768
BsuTUI ATCGAT 1 cut(s) 38
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 2 cut(s) 301, 316
BtsCI GGATG 1 cut(s) 141
BtsI GCAGTG 2 cut(s) 401, 737
BtsIMutI CAGTG 3 cut(s) 401, 621, 737
Cac8I GCNNGC 3 cut(s) 345, 613, 766
CfoI GCGC 4 cut(s) 47, 75, 170, 343
Cfr10I RCCGGY 1 cut(s) 764
Cfr13I GGNCC 1 cut(s) 671
ClaI ATCGAT 1 cut(s) 38
Csp6I GTAC 3 cut(s) 553, 797, 855
CviAII CATG 4 cut(s) 22, 352, 710, 842
CviQI GTAC 3 cut(s) 553, 797, 855
DdeI CTNAG 2 cut(s) 495, 723
DpnI GATC 1 cut(s) 215
DpnII GATC 1 cut(s) 213
DrdI GACNNNNNNGTC 1 cut(s) 380
DseDI GACNNNNNNGTC 1 cut(s) 380
EaeI YGGCCR 2 cut(s) 157, 318
Eco47III AGCGCT 2 cut(s) 46, 74
Eco57I CTGAAG 2 cut(s) 211, 645
Eco91I GGTNACC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 843
EcoO65I GGTNACC 1 cut(s) 283
EcoT22I ATGCAT 1 cut(s) 667
FaeI CATG 4 cut(s) 25, 355, 713, 845
FaiI YATR 5 cut(s) 23, 353, 381, 711, 843
FaqI GGGAC 3 cut(s) 14, 86, 250
FatI CATG 4 cut(s) 21, 351, 709, 841
FauI CCCGC 1 cut(s) 641
Fnu4HI GCNGC 3 cut(s) 166, 508, 806
FokI GGATG 1 cut(s) 128
Fsp4HI GCNGC 3 cut(s) 166, 508, 806
FspBI CTAG 2 cut(s) 602, 743
GlaI GCGC 4 cut(s) 46, 74, 169, 342
GluI GCNGC 3 cut(s) 166, 508, 806
GsuI CTGGAG 4 cut(s) 118, 192, 205, 511
HaeII RGCGCY 4 cut(s) 48, 76, 171, 344
HaeIII GGCC 6 cut(s) 159, 320, 584, 673, 750, 768
HapII CCGG 2 cut(s) 435, 765
HhaI GCGC 4 cut(s) 47, 75, 170, 343
Hin1II CATG 4 cut(s) 25, 355, 713, 845
Hin6I GCGC 4 cut(s) 45, 73, 168, 341
HinP1I GCGC 4 cut(s) 45, 73, 168, 341
HincII GTYRAC 2 cut(s) 306, 420
HindII GTYRAC 2 cut(s) 306, 420
HinfI GANTC 3 cut(s) 5, 468, 898
HpaII CCGG 2 cut(s) 435, 765
HphI GGTGA 2 cut(s) 277, 424
Hpy166II GTNNAC 3 cut(s) 306, 420, 432
Hpy188I TCNGA 1 cut(s) 146
Hpy188III TCNNGA 7 cut(s) 94, 385, 528, 650, 818, 902, 916
Hpy8I GTNNAC 3 cut(s) 306, 420, 432
Hpy99I CGWCG 2 cut(s) 158, 302
HpyAV CCTTC 2 cut(s) 397, 740
HpyCH4III ACNGT 5 cut(s) 100, 197, 445, 571, 593
HpyCH4IV ACGT 3 cut(s) 81, 300, 315
HpyCH4V TGCA 6 cut(s) 25, 264, 665, 737, 845, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 326, 491
HpyF3I CTNAG 2 cut(s) 495, 723
HpySE526I ACGT 3 cut(s) 81, 300, 315
Hsp92II CATG 4 cut(s) 25, 355, 713, 845
HspAI GCGC 4 cut(s) 45, 73, 168, 341
KroI GCCGGC 1 cut(s) 764
KroNI GCCGGC 1 cut(s) 766
Kzo9I GATC 1 cut(s) 213
LmnI GCTCC 2 cut(s) 173, 761
Lsp1109I GCAGC 2 cut(s) 494, 817
LweI GCATC 2 cut(s) 169, 652
MaeI CTAG 2 cut(s) 602, 743
MaeII ACGT 3 cut(s) 81, 300, 315
MaeIII GTNAC 3 cut(s) 283, 371, 407
MalI GATC 1 cut(s) 215
MboI GATC 1 cut(s) 213
MboII GAAGA 4 cut(s) 268, 412, 477, 638
MhlI GDGCHC 3 cut(s) 21, 617, 834
MluCI AATT 2 cut(s) 545, 578
MmeI TCCRAC 1 cut(s) 612
MnlI CCTC 7 cut(s) 95, 351, 490, 595, 693, 849, 898
Mph1103I ATGCAT 1 cut(s) 667
MroNI GCCGGC 1 cut(s) 764
MseI TTAA 2 cut(s) 690, 946
MslI CAYNNNNRTG 2 cut(s) 14, 714
MspA1I CMGCKG 1 cut(s) 808
MspI CCGG 2 cut(s) 435, 765
MwoI GCNNNNNNNGC 3 cut(s) 165, 326, 491
NaeI GCCGGC 1 cut(s) 766
NdeII GATC 1 cut(s) 213
NgoMIV GCCGGC 1 cut(s) 764
NlaIII CATG 4 cut(s) 25, 355, 713, 845
NlaIV GGNNCC 2 cut(s) 672, 763
NmuCI GTSAC 2 cut(s) 283, 371
NsiI ATGCAT 1 cut(s) 667
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PdiI GCCGGC 1 cut(s) 766
PfeI GAWTC 2 cut(s) 468, 898
PkrI GCNGC 3 cut(s) 167, 509, 807
PspEI GGTNACC 1 cut(s) 283
PspN4I GGNNCC 2 cut(s) 672, 763
PspPI GGNCC 1 cut(s) 671
PsrI GAACNNNNNNTAC 2 cut(s) 780, 812
PvuII CAGCTG 1 cut(s) 808
RsaI GTAC 3 cut(s) 554, 798, 856
RsaNI GTAC 3 cut(s) 553, 797, 855
RseI CAYNNNNRTG 2 cut(s) 14, 714
SaqAI TTAA 2 cut(s) 690, 946
SatI GCNGC 3 cut(s) 166, 508, 806
Sau3AI GATC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 671
SduI GDGCHC 3 cut(s) 21, 617, 834
SfaNI GCATC 2 cut(s) 169, 652
SmiMI CAYNNNNRTG 2 cut(s) 14, 714
SmlI CTYRAG 2 cut(s) 86, 902
SmoI CTYRAG 2 cut(s) 86, 902
Sse9I AATT 2 cut(s) 545, 578
SsiI CCGC 5 cut(s) 165, 347, 355, 376, 634
SspMI CTAG 2 cut(s) 602, 743
TaaI ACNGT 5 cut(s) 100, 197, 445, 571, 593
TaiI ACGT 3 cut(s) 84, 303, 318
TaqI TCGA 3 cut(s) 38, 66, 192
TaqII GACCGA 1 cut(s) 323
TasI AATT 2 cut(s) 545, 578
TatI WGTACW 1 cut(s) 552
TauI GCSGC 1 cut(s) 168
TfiI GAWTC 2 cut(s) 468, 898
Tru1I TTAA 2 cut(s) 690, 946
Tru9I TTAA 2 cut(s) 690, 946
TscAI CASTG 3 cut(s) 401, 628, 744
TseFI GTSAC 2 cut(s) 283, 371
TseI GCWGC 2 cut(s) 507, 805
Tsp45I GTSAC 2 cut(s) 283, 371
TspDTI ATGAA 2 cut(s) 109, 801
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 3 cut(s) 401, 628, 744
XagI CCTNNNNNAGG 1 cut(s) 843
XcmI CCANNNNNNNNNTGG 1 cut(s) 167
XspI CTAG 2 cut(s) 602, 743
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.