In Between Ring fingers
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 759 |
| AciI | CCGC | 1 cut(s) | 225 |
| AfaI | GTAC | 1 cut(s) | 281 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 56, 442, 640 |
| AgsI | TTSAA | 1 cut(s) | 515 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 600, 632 |
| AluBI | AGCT | 2 cut(s) | 639, 731 |
| AluI | AGCT | 2 cut(s) | 639, 731 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 185, 774 |
| AsuHPI | GGTGA | 3 cut(s) | 322, 465, 473 |
| AsuII | TTCGAA | 1 cut(s) | 541 |
| BaeGI | GKGCMC | 2 cut(s) | 635, 748 |
| BccI | CCATC | 2 cut(s) | 347, 490 |
| BclI | TGATCA | 1 cut(s) | 114 |
| BfaI | CTAG | 1 cut(s) | 108 |
| BfmI | CTRYAG | 2 cut(s) | 324, 646 |
| BmiI | GGNNCC | 1 cut(s) | 358 |
| BmsI | GCATC | 5 cut(s) | 146, 542, 605, 658, 706 |
| Bpu14I | TTCGAA | 1 cut(s) | 541 |
| Bsa29I | ATCGAT | 1 cut(s) | 792 |
| BsaAI | YACGTR | 1 cut(s) | 622 |
| BsaBI | GATNNNNATC | 1 cut(s) | 195 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 56, 442, 640 |
| Bse1I | ACTGG | 1 cut(s) | 263 |
| Bse8I | GATNNNNATC | 1 cut(s) | 195 |
| BseCI | ATCGAT | 1 cut(s) | 792 |
| BseGI | GGATG | 3 cut(s) | 137, 557, 596 |
| BseJI | GATNNNNATC | 1 cut(s) | 195 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 56, 442, 640 |
| BseMII | CTCAG | 2 cut(s) | 84, 249 |
| BseNI | ACTGG | 1 cut(s) | 263 |
| BseSI | GKGCMC | 2 cut(s) | 635, 748 |
| BseYI | CCCAGC | 1 cut(s) | 635 |
| Bsh1285I | CGRYCG | 1 cut(s) | 796 |
| BshVI | ATCGAT | 1 cut(s) | 792 |
| BsiEI | CGRYCG | 1 cut(s) | 796 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 56, 442, 640 |
| Bsp119I | TTCGAA | 1 cut(s) | 541 |
| Bsp1286I | GDGCHC | 2 cut(s) | 635, 748 |
| Bsp143I | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| BspACI | CCGC | 1 cut(s) | 225 |
| BspCNI | CTCAG | 2 cut(s) | 83, 250 |
| BspDI | ATCGAT | 1 cut(s) | 792 |
| BspLI | GGNNCC | 1 cut(s) | 358 |
| BspMAI | CTGCAG | 2 cut(s) | 328, 650 |
| BspT104I | TTCGAA | 1 cut(s) | 541 |
| BsrI | ACTGG | 1 cut(s) | 263 |
| BssMI | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| Bst4CI | ACNGT | 2 cut(s) | 761, 784 |
| Bst6I | CTCTTC | 3 cut(s) | 325, 367, 373 |
| BstBAI | YACGTR | 1 cut(s) | 622 |
| BstBI | TTCGAA | 1 cut(s) | 541 |
| BstDEI | CTNAG | 3 cut(s) | 8, 70, 258 |
| BstF5I | GGATG | 3 cut(s) | 137, 557, 596 |
| BstKTI | GATC | 5 cut(s) | 55, 117, 193, 792, 796 |
| BstMBI | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| BstMCI | CGRYCG | 1 cut(s) | 796 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 143, 398, 576, 645 |
| BstNSI | RCATGY | 1 cut(s) | 593 |
| BstSFI | CTRYAG | 2 cut(s) | 324, 646 |
| BstSLI | GKGCMC | 2 cut(s) | 635, 748 |
| Bsu15I | ATCGAT | 1 cut(s) | 792 |
| BsuTUI | ATCGAT | 1 cut(s) | 792 |
| BtsCI | GGATG | 3 cut(s) | 137, 557, 596 |
| BtsIMutI | CAGTG | 1 cut(s) | 775 |
| CaiI | CAGNNNCTG | 2 cut(s) | 185, 774 |
| ClaI | ATCGAT | 1 cut(s) | 792 |
| CseI | GACGC | 1 cut(s) | 171 |
| Csp6I | GTAC | 1 cut(s) | 280 |
| CviAII | CATG | 3 cut(s) | 160, 422, 590 |
| CviJI | RGCY | 7 cut(s) | 107, 262, 308, 527, 639, 653, 731 |
| CviKI_1 | RGCY | 7 cut(s) | 107, 262, 308, 527, 639, 653, 731 |
| CviQI | GTAC | 1 cut(s) | 280 |
| DdeI | CTNAG | 3 cut(s) | 8, 70, 258 |
| DpnI | GATC | 5 cut(s) | 54, 116, 192, 791, 795 |
| DpnII | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 759 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 759 |
| Eam1104I | CTCTTC | 3 cut(s) | 325, 367, 373 |
| EarI | CTCTTC | 3 cut(s) | 325, 367, 373 |
| FaeI | CATG | 3 cut(s) | 163, 425, 593 |
| FaiI | YATR | 8 cut(s) | 161, 249, 423, 581, 591, 677, 701, 734 |
| FatI | CATG | 3 cut(s) | 159, 421, 589 |
| FbaI | TGATCA | 1 cut(s) | 114 |
| FokI | GGATG | 3 cut(s) | 124, 564, 583 |
| FspBI | CTAG | 1 cut(s) | 108 |
| GsaI | CCCAGC | 1 cut(s) | 639 |
| HgaI | GACGC | 1 cut(s) | 171 |
| Hin1II | CATG | 3 cut(s) | 163, 425, 593 |
| HinfI | GANTC | 5 cut(s) | 254, 272, 455, 464, 753 |
| HphI | GGTGA | 3 cut(s) | 322, 465, 473 |
| Hpy166II | GTNNAC | 1 cut(s) | 559 |
| Hpy188I | TCNGA | 6 cut(s) | 73, 187, 201, 259, 271, 574 |
| Hpy188III | TCNNGA | 1 cut(s) | 443 |
| Hpy8I | GTNNAC | 1 cut(s) | 559 |
| HpyAV | CCTTC | 2 cut(s) | 574, 798 |
| HpyCH4III | ACNGT | 2 cut(s) | 761, 784 |
| HpyCH4IV | ACGT | 1 cut(s) | 621 |
| HpyCH4V | TGCA | 4 cut(s) | 295, 326, 579, 648 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 143, 398, 576, 645 |
| HpyF3I | CTNAG | 3 cut(s) | 8, 70, 258 |
| HpySE526I | ACGT | 1 cut(s) | 621 |
| Hsp92II | CATG | 3 cut(s) | 163, 425, 593 |
| Ksp22I | TGATCA | 1 cut(s) | 114 |
| Kzo9I | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| LpnPI | CCDG | 8 cut(s) | 63, 276, 428, 444, 634, 644, 649, 685 |
| LweI | GCATC | 5 cut(s) | 146, 542, 605, 658, 706 |
| MaeI | CTAG | 1 cut(s) | 108 |
| MaeII | ACGT | 1 cut(s) | 621 |
| MaeIII | GTNAC | 3 cut(s) | 328, 471, 770 |
| MalI | GATC | 5 cut(s) | 54, 116, 192, 791, 795 |
| MboI | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| MboII | GAAGA | 5 cut(s) | 86, 185, 312, 354, 360 |
| MhlI | GDGCHC | 2 cut(s) | 635, 748 |
| MluCI | AATT | 1 cut(s) | 438 |
| MlyI | GAGTC | 3 cut(s) | 263, 464, 747 |
| MmeI | TCCRAC | 2 cut(s) | 180, 435 |
| MnlI | CCTC | 8 cut(s) | 11, 298, 328, 343, 370, 376, 517, 541 |
| MseI | TTAA | 1 cut(s) | 33 |
| MslI | CAYNNNNRTG | 2 cut(s) | 201, 351 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 143, 398, 576, 645 |
| NdeII | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| NlaIII | CATG | 3 cut(s) | 163, 425, 593 |
| NlaIV | GGNNCC | 1 cut(s) | 358 |
| NmuCI | GTSAC | 3 cut(s) | 328, 471, 770 |
| NspI | RCATGY | 1 cut(s) | 593 |
| NspV | TTCGAA | 1 cut(s) | 541 |
| PfeI | GAWTC | 2 cut(s) | 272, 464 |
| Ple19I | CGATCG | 1 cut(s) | 796 |
| PleI | GAGTC | 3 cut(s) | 262, 463, 747 |
| PpsI | GAGTC | 3 cut(s) | 262, 463, 747 |
| Ppu21I | YACGTR | 1 cut(s) | 622 |
| PspFI | CCCAGC | 1 cut(s) | 635 |
| PspN4I | GGNNCC | 1 cut(s) | 358 |
| PstI | CTGCAG | 2 cut(s) | 328, 650 |
| PstNI | CAGNNNCTG | 2 cut(s) | 185, 774 |
| PvuI | CGATCG | 1 cut(s) | 796 |
| RsaI | GTAC | 1 cut(s) | 281 |
| RsaNI | GTAC | 1 cut(s) | 280 |
| RseI | CAYNNNNRTG | 2 cut(s) | 201, 351 |
| SaqAI | TTAA | 1 cut(s) | 33 |
| Sau3AI | GATC | 5 cut(s) | 52, 114, 190, 789, 793 |
| SchI | GAGTC | 3 cut(s) | 263, 464, 747 |
| SduI | GDGCHC | 2 cut(s) | 635, 748 |
| SetI | ASST | 8 cut(s) | 9, 52, 335, 624, 641, 710, 733, 809 |
| SfaNI | GCATC | 5 cut(s) | 146, 542, 605, 658, 706 |
| SfcI | CTRYAG | 2 cut(s) | 324, 646 |
| SfuI | TTCGAA | 1 cut(s) | 541 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 201, 351 |
| Sse9I | AATT | 1 cut(s) | 438 |
| SsiI | CCGC | 1 cut(s) | 225 |
| SspMI | CTAG | 1 cut(s) | 108 |
| TaaI | ACNGT | 2 cut(s) | 761, 784 |
| TaiI | ACGT | 1 cut(s) | 624 |
| TaqI | TCGA | 3 cut(s) | 541, 792, 796 |
| TasI | AATT | 1 cut(s) | 438 |
| TfiI | GAWTC | 2 cut(s) | 272, 464 |
| Tru1I | TTAA | 1 cut(s) | 33 |
| Tru9I | TTAA | 1 cut(s) | 33 |
| TscAI | CASTG | 1 cut(s) | 775 |
| TseFI | GTSAC | 3 cut(s) | 328, 471, 770 |
| Tsp45I | GTSAC | 3 cut(s) | 328, 471, 770 |
| TspDTI | ATGAA | 6 cut(s) | 17, 185, 255, 360, 438, 533 |
| TspRI | CASTG | 1 cut(s) | 775 |
| XceI | RCATGY | 1 cut(s) | 593 |
| XspI | CTAG | 1 cut(s) | 108 |