FvH4_3g37483

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000315
Physical Location & Seq
Forward (+)
32182869 .. 32189940
7072 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g37483.t1

Sequence Viewer

Length: 852 bp
ATGAAGATTTGGATTGGGATATGCATCAATTATCCAGTGCAGCTTGTCAGGATTGCTAGGTTGTCCTGCCTCTCTGATCTCTATGTAGCTCTGGTTTATTGCAGTACATCATCTACAGAATGGAATTGTATATGGATGTGTCCAGGTGGTAAGGCAAACAGGGTTGCCGCCCTTTCGGTTCATGAGGCGGGTGCTGAGAGAGCTTGTTGCATTGTTGGAAATGGGGGATTACGATTCCCAGAGATCTCAGTTTCTTATGATAACTGGATTTGGCGCAAGATTGTTGGAGGTGTCAACAAGATGGGATCCCCCTCCCATAGTCATATGGGATTACCAGAACTCGAGAGAATCGCTGCAACGTTCGAGGAGGGATATATGCTGTTGCCTCAAGCCAGTCCTTTTCCTCAAGGTCGAGAGCCGTCAATGGAAGCAGGAGATTGGAGGGGCAAATGGCCCGCCGATATGCGGCAAAGTGTTATCAATAAGATAATATCTACATTGAAGACGGCTTGCCCCATCTCTGGTCAAGAGGGATTAGAAGAACTCGAGAGAAGTGCTGCAAGTTTGGAGGAAAAGTCTTATGCTGCTGCCAATAGCCAGTTGGACTATCTACAGAAAAATTCTCAGGAGATGGTCACAGTGGTGACCAAGCCACAGAACACAATATCCAATTCTCTAGAATCCAATGGCAAGGGACTTCTTGTTTCAGTCCCCGATCAAGGACACTCACTCACTATGTCACTGCCAGCCATTCAGTCCCAAACAAGGCAACAATTTACATACAGAATAACATTCATCCTGCCAGAGTTCAAAGTTTTGCGGGGATATCATCTGCACTTCCTTCTACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

284

Amino Acids

31.4

Weight (kDa)

7.58

Isoelectric Point (pI)

52.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 145 - 224 7.6e-20 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 168, 188, 456, 466, 820
AclI AACGTT 1 cut(s) 359
AclWI GGATC 2 cut(s) 300, 313
AcsI RAATTY 1 cut(s) 619
AfaI GTAC 1 cut(s) 106
AfiI CCNNNNNNNGG 4 cut(s) 465, 521, 719, 765
AgsI TTSAA 2 cut(s) 502, 811
AjnI CCWGG 1 cut(s) 142
AleI CACNNNNGTG 1 cut(s) 641
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 3 cut(s) 43, 89, 203
AluI AGCT 3 cut(s) 43, 89, 203
AlwI GGATC 2 cut(s) 300, 313
Ama87I CYCGRG 2 cut(s) 341, 545
AoxI GGCC 1 cut(s) 452
ApeKI GCWGC 5 cut(s) 40, 353, 557, 584, 587
ApoI RAATTY 1 cut(s) 619
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 453
AsuHPI GGTGA 1 cut(s) 655
AvaI CYCGRG 2 cut(s) 341, 545
BamHI GGATCC 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 509
BbvI GCAGC 5 cut(s) 52, 340, 544, 571, 574
BccI CCATC 3 cut(s) 295, 524, 625
BceAI ACGGC 2 cut(s) 403, 522
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BciT130I CCWGG 1 cut(s) 144
BfaI CTAG 2 cut(s) 57, 677
BfmI CTRYAG 2 cut(s) 114, 611
BglII AGATCT 1 cut(s) 243
BisI GCNGC 7 cut(s) 41, 168, 354, 467, 558, 585, 588
BlsI GCNGC 7 cut(s) 42, 169, 355, 468, 559, 586, 589
Bme1390I CCNGG 1 cut(s) 144
BmeT110I CYCGRG 2 cut(s) 341, 545
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 509
BpuEI CTTGAG 2 cut(s) 372, 390
BsaBI GATNNNNATC 1 cut(s) 23
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 4 cut(s) 465, 521, 719, 765
Bse1I ACTGG 4 cut(s) 35, 269, 393, 598
Bse8I GATNNNNATC 1 cut(s) 23
BseBI CCWGG 1 cut(s) 144
BseGI GGATG 2 cut(s) 141, 795
BseJI GATNNNNATC 1 cut(s) 23
BseLI CCNNNNNNNGG 4 cut(s) 465, 521, 719, 765
BseMII CTCAG 3 cut(s) 186, 261, 638
BseNI ACTGG 4 cut(s) 35, 269, 393, 598
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 5 cut(s) 52, 340, 544, 571, 574
BsgI GTGCAG 2 cut(s) 59, 818
BshFI GGCC 1 cut(s) 454
BsiHKCI CYCGRG 2 cut(s) 341, 545
BslFI GGGAC 3 cut(s) 695, 708, 742
BslI CCNNNNNNNGG 4 cut(s) 465, 521, 719, 765
BsmFI GGGAC 3 cut(s) 695, 708, 742
BsnI GGCC 1 cut(s) 454
BsoBI CYCGRG 2 cut(s) 341, 545
Bsp143I GATC 4 cut(s) 76, 243, 305, 715
BspACI CCGC 5 cut(s) 168, 188, 456, 466, 820
BspANI GGCC 1 cut(s) 454
BspCNI CTCAG 3 cut(s) 187, 260, 637
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 307
BspPI GGATC 2 cut(s) 300, 313
BsrI ACTGG 4 cut(s) 35, 269, 393, 598
BssMI GATC 4 cut(s) 76, 243, 305, 715
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 640
BstC8I GCNNGC 3 cut(s) 456, 511, 747
BstDEI CTNAG 3 cut(s) 195, 247, 624
BstEII GGTNACC 1 cut(s) 643
BstF5I GGATG 2 cut(s) 141, 795
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 4 cut(s) 79, 246, 308, 718
BstMBI GATC 4 cut(s) 76, 243, 305, 715
BstMWI GCNNNNNNNGC 1 cut(s) 200
BstNI CCWGG 1 cut(s) 144
BstPI GGTNACC 1 cut(s) 643
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 2 cut(s) 114, 611
BstV1I GCAGC 5 cut(s) 52, 340, 544, 571, 574
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 2 cut(s) 243, 305
BstYI RGATCY 2 cut(s) 243, 305
BsuRI GGCC 1 cut(s) 454
BtsCI GGATG 2 cut(s) 141, 795
BtsI GCAGTG 1 cut(s) 740
BtsIMutI CAGTG 3 cut(s) 42, 645, 740
Cac8I GCNNGC 3 cut(s) 456, 511, 747
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 453
Csp6I GTAC 1 cut(s) 105
CviAII CATG 2 cut(s) 182, 849
CviQI GTAC 1 cut(s) 105
DdeI CTNAG 3 cut(s) 195, 247, 624
DpnI GATC 4 cut(s) 78, 245, 307, 717
DpnII GATC 4 cut(s) 76, 243, 305, 715
Eco32I GATATC 1 cut(s) 827
Eco88I CYCGRG 2 cut(s) 341, 545
Eco91I GGTNACC 1 cut(s) 643
EcoO65I GGTNACC 1 cut(s) 643
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 1 cut(s) 827
EcoT22I ATGCAT 1 cut(s) 26
FaeI CATG 2 cut(s) 185, 852
FaqI GGGAC 3 cut(s) 695, 708, 742
FatI CATG 2 cut(s) 181, 848
FauI CCCGC 3 cut(s) 181, 463, 813
FauNDI CATATG 1 cut(s) 324
Fnu4HI GCNGC 7 cut(s) 41, 168, 354, 467, 558, 585, 588
FokI GGATG 2 cut(s) 148, 782
Fsp4HI GCNGC 7 cut(s) 41, 168, 354, 467, 558, 585, 588
FspBI CTAG 2 cut(s) 57, 677
GlaI GCGC 1 cut(s) 275
GluI GCNGC 7 cut(s) 41, 168, 354, 467, 558, 585, 588
HaeIII GGCC 1 cut(s) 454
HhaI GCGC 1 cut(s) 276
Hin1II CATG 2 cut(s) 185, 852
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 3 cut(s) 234, 348, 680
HphI GGTGA 1 cut(s) 655
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 8 cut(s) 49, 182, 343, 413, 527, 547, 626, 677
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 1 cut(s) 851
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 1 cut(s) 359
HpyCH4V TGCA 7 cut(s) 24, 40, 102, 210, 356, 560, 835
HpyF10VI GCNNNNNNNGC 1 cut(s) 200
HpyF3I CTNAG 3 cut(s) 195, 247, 624
HpySE526I ACGT 1 cut(s) 359
Hsp92II CATG 2 cut(s) 185, 852
HspAI GCGC 1 cut(s) 274
Kzo9I GATC 4 cut(s) 76, 243, 305, 715
Lsp1109I GCAGC 5 cut(s) 52, 340, 544, 571, 574
LweI GCATC 1 cut(s) 33
MaeI CTAG 2 cut(s) 57, 677
MaeII ACGT 1 cut(s) 359
MaeIII GTNAC 3 cut(s) 634, 643, 738
MalI GATC 4 cut(s) 78, 245, 307, 717
MboI GATC 4 cut(s) 76, 243, 305, 715
MboII GAAGA 3 cut(s) 16, 514, 551
MflI RGATCY 2 cut(s) 243, 305
MluCI AATT 5 cut(s) 28, 124, 619, 670, 773
MmeI TCCRAC 3 cut(s) 196, 265, 582
Mph1103I ATGCAT 1 cut(s) 26
MslI CAYNNNNRTG 1 cut(s) 641
MspR9I CCNGG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 200
NdeI CATATG 1 cut(s) 324
NdeII GATC 4 cut(s) 76, 243, 305, 715
NlaIII CATG 2 cut(s) 185, 852
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 3 cut(s) 634, 643, 738
NsiI ATGCAT 1 cut(s) 26
OliI CACNNNNGTG 1 cut(s) 641
PaeR7I CTCGAG 2 cut(s) 341, 545
PagI TCATGA 1 cut(s) 181
PfeI GAWTC 3 cut(s) 234, 348, 680
PkrI GCNGC 7 cut(s) 42, 169, 355, 468, 559, 586, 589
Psp1406I AACGTT 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 142
PspEI GGTNACC 1 cut(s) 643
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 453
PsuI RGATCY 2 cut(s) 243, 305
RsaI GTAC 1 cut(s) 106
RsaNI GTAC 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 641
SatI GCNGC 7 cut(s) 41, 168, 354, 467, 558, 585, 588
Sau3AI GATC 4 cut(s) 76, 243, 305, 715
Sau96I GGNCC 1 cut(s) 453
ScrFI CCNGG 1 cut(s) 144
SetI ASST 8 cut(s) 45, 62, 91, 148, 205, 292, 362, 412
SfaNI GCATC 1 cut(s) 33
SfcI CTRYAG 2 cut(s) 114, 611
Sfr274I CTCGAG 2 cut(s) 341, 545
SlaI CTCGAG 2 cut(s) 341, 545
SmiMI CAYNNNNRTG 1 cut(s) 641
SmlI CTYRAG 4 cut(s) 341, 387, 405, 545
SmoI CTYRAG 4 cut(s) 341, 387, 405, 545
Sse9I AATT 5 cut(s) 28, 124, 619, 670, 773
SsiI CCGC 5 cut(s) 168, 188, 456, 466, 820
SspMI CTAG 2 cut(s) 57, 677
StyD4I CCNGG 1 cut(s) 142
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 1 cut(s) 362
TaqI TCGA 4 cut(s) 342, 363, 412, 546
TasI AATT 5 cut(s) 28, 124, 619, 670, 773
TatI WGTACW 1 cut(s) 104
TauI GCSGC 2 cut(s) 170, 469
TfiI GAWTC 3 cut(s) 234, 348, 680
TscAI CASTG 3 cut(s) 42, 645, 747
TseFI GTSAC 3 cut(s) 634, 643, 738
TseI GCWGC 5 cut(s) 40, 353, 557, 584, 587
Tsp45I GTSAC 3 cut(s) 634, 643, 738
TspDTI ATGAA 3 cut(s) 17, 170, 784
TspRI CASTG 3 cut(s) 42, 645, 747
XapI RAATTY 1 cut(s) 619
XbaI TCTAGA 1 cut(s) 676
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XhoI CTCGAG 2 cut(s) 341, 545
XspI CTAG 2 cut(s) 57, 677
Zsp2I ATGCAT 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.