Rh5CG544500

KIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000315
Physical Location & Seq
Forward (+)
76012207 .. 76015340
3134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG544500.1

Sequence Viewer

Length: 774 bp
ATGTGGTTTAACTTTTGTGTGAATCTCCTTGTCAAGTCGGATTATGAACGAAAAATTTCTCTGAAGTTGCTCACAATGGAGACCAAGTCGCAGAACACAAGGGGCAGCTCTATACAATTCAACTCAGCCAGCAATAGCAAGAGACTCCCCGATCCTGTTTTAATGAATACCAGTAACAATGGGTGGCCTCCTCAAGGTGGAGGACCATCTATGGACACATCAGATTGGAGTCAATTGCCGTCAGATGCACAACATGGATGGCAACAGGGAGCTCCCCAACAGCAACGGCAGCAAACCTCAAATTCTCAGCAGTACTATATTTACTCGCAGCAGTATATGTACCAGCAACAGTTACAACAACAACTCCTCCTTCAAAGTCATATTCAACTGCAACAAGAGCAGCAGCAGATTCCACTACAAAAAACTCAGTTGCAATCTTCTCAGCAATCAGCATCTGTTATGCAACCTTCGGTGGAGCCATTGCATCTCTCTGTGCAACCTTCTGAGCAATTGCCGCCAGATGCGCGACATAGAATTGTCAACAAAATAACTAATGTGCTAAAGAGGAATCCGTCCTGTGGTCAAATGGGATTACCAGAACTCGAGAGAATCGCAGCAACACTTGAGGAGAAGATATCTGCCGTTGCCTCAAGCCAGGCGGATTATCTGCGTAAAGTAAGTCTGAAGATGCTCACCATGGAGACGCAAATCACAGCTCCCCCATCCAATGACAGTTTGGAATCATTATTTGCTTCCCTGGATTTGCAGGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

257

Amino Acids

29.06

Weight (kDa)

6.43

Isoelectric Point (pI)

67.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 169 - 242 5.2e-20 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 526
AciI CCGC 2 cut(s) 515, 659
AclWI GGATC 1 cut(s) 146
AcsI RAATTY 2 cut(s) 54, 301
AcuI CTGAAG 2 cut(s) 83, 704
AfaI GTAC 2 cut(s) 314, 341
AfiI CCNNNNNNNGG 3 cut(s) 194, 197, 578
AgsI TTSAA 3 cut(s) 121, 374, 386
AjnI CCWGG 2 cut(s) 654, 756
AluBI AGCT 3 cut(s) 108, 272, 716
AluI AGCT 3 cut(s) 108, 272, 716
Alw21I GWGCWC 1 cut(s) 274
Alw26I GTCTC 3 cut(s) 74, 136, 695
AlwI GGATC 1 cut(s) 146
AlwNI CAGNNNCTG 1 cut(s) 455
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 6 cut(s) 105, 289, 328, 400, 403, 614
ApoI RAATTY 2 cut(s) 54, 301
Asp700I GAANNNNTTC 1 cut(s) 55
AspLEI GCGC 1 cut(s) 526
AspS9I GGNCC 1 cut(s) 203
AsuHPI GGTGA 1 cut(s) 685
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 1 cut(s) 203
BanII GRGCYC 1 cut(s) 274
Bbv12I GWGCWC 1 cut(s) 274
BbvI GCAGC 6 cut(s) 117, 301, 340, 412, 415, 626
BccI CCATC 3 cut(s) 214, 252, 730
BceAI ACGGC 3 cut(s) 223, 302, 626
BciT130I CCWGG 2 cut(s) 656, 758
BcoDI GTCTC 3 cut(s) 74, 136, 695
BfaI CTAG 1 cut(s) 772
BisI GCNGC 7 cut(s) 106, 290, 329, 401, 404, 515, 615
BlsI GCNGC 7 cut(s) 107, 291, 330, 402, 405, 516, 616
BmcAI AGTACT 1 cut(s) 314
Bme1390I CCNGG 2 cut(s) 656, 758
Bme18I GGWCC 1 cut(s) 203
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 1 cut(s) 203
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 2 cut(s) 656, 758
BmsI GCATC 5 cut(s) 235, 461, 493, 511, 678
BpuEI CTTGAG 3 cut(s) 177, 634, 644
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 696, 756
BsaXI ACNNNNNCTCC 6 cut(s) 71, 101, 348, 351, 378, 381
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 197, 578
Bse1I ACTGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 479
BseBI CCWGG 2 cut(s) 656, 758
BseDI CCNNGG 2 cut(s) 696, 756
BseGI GGATG 2 cut(s) 263, 722
BseLI CCNNNNNNNGG 3 cut(s) 194, 197, 578
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 5 cut(s) 138, 320, 440, 455, 495
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 3 cut(s) 180, 356, 641
BseXI GCAGC 6 cut(s) 117, 301, 340, 412, 415, 626
Bsh1236I CGCG 1 cut(s) 526
BshFI GGCC 1 cut(s) 187
BsiHKAI GWGCWC 1 cut(s) 274
BsiHKCI CYCGRG 1 cut(s) 602
BslI CCNNNNNNNGG 3 cut(s) 194, 197, 578
BsmAI GTCTC 3 cut(s) 74, 136, 695
BsmBI CGTCTC 1 cut(s) 695
BsnI GGCC 1 cut(s) 187
Bso31I GGTCTC 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 602
Bsp1286I GDGCHC 1 cut(s) 274
Bsp143I GATC 1 cut(s) 151
Bsp19I CCATGG 1 cut(s) 696
BspACI CCGC 2 cut(s) 515, 659
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 5 cut(s) 137, 319, 439, 454, 496
BspFNI CGCG 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 477
BspPI GGATC 1 cut(s) 146
BspTNI GGTCTC 1 cut(s) 74
BsrDI GCAATG 1 cut(s) 479
BsrI ACTGG 1 cut(s) 171
BssECI CCNNGG 2 cut(s) 696, 756
BssMI GATC 1 cut(s) 151
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 2 cut(s) 656, 758
Bst4CI ACNGT 2 cut(s) 351, 734
BstC8I GCNNGC 1 cut(s) 130
BstDEI CTNAG 5 cut(s) 124, 306, 426, 441, 504
BstDSI CCRYGG 1 cut(s) 696
BstENI CCTNNNNNAGG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 263, 722
BstFNI CGCG 1 cut(s) 526
BstHHI GCGC 1 cut(s) 526
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 3 cut(s) 74, 136, 695
BstMBI GATC 1 cut(s) 151
BstMWI GCNNNNNNNGC 4 cut(s) 289, 397, 514, 523
BstNI CCWGG 2 cut(s) 656, 758
BstSCI CCNGG 2 cut(s) 654, 756
BstUI CGCG 1 cut(s) 526
BstV1I GCAGC 6 cut(s) 117, 301, 340, 412, 415, 626
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 696
BtsCI GGATG 2 cut(s) 263, 722
Cac8I GCNNGC 1 cut(s) 130
CaiI CAGNNNCTG 1 cut(s) 455
CfoI GCGC 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 203
CseI GACGC 1 cut(s) 712
Csp6I GTAC 2 cut(s) 313, 340
CviAII CATG 2 cut(s) 254, 697
CviJI RGCY 7 cut(s) 108, 128, 187, 272, 478, 654, 716
CviKI_1 RGCY 7 cut(s) 108, 128, 187, 272, 478, 654, 716
CviQI GTAC 2 cut(s) 313, 340
DdeI CTNAG 5 cut(s) 124, 306, 426, 441, 504
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
EciI GGCGGA 1 cut(s) 674
Ecl136II GAGCTC 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 696
Eco24I GRGCYC 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 74
Eco32I GATATC 1 cut(s) 636
Eco47I GGWCC 1 cut(s) 203
Eco53kI GAGCTC 1 cut(s) 272
Eco57I CTGAAG 2 cut(s) 83, 704
Eco88I CYCGRG 1 cut(s) 602
EcoICRI GAGCTC 1 cut(s) 272
EcoNI CCTNNNNNAGG 1 cut(s) 192
EcoRII CCWGG 2 cut(s) 654, 756
EcoRV GATATC 1 cut(s) 636
EcoT14I CCWWGG 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 274
ErhI CCWWGG 1 cut(s) 696
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 2 cut(s) 257, 700
FatI CATG 2 cut(s) 253, 696
Fnu4HI GCNGC 7 cut(s) 106, 290, 329, 401, 404, 515, 615
FokI GGATG 2 cut(s) 270, 709
FriOI GRGCYC 1 cut(s) 274
Fsp4HI GCNGC 7 cut(s) 106, 290, 329, 401, 404, 515, 615
FspBI CTAG 1 cut(s) 772
GlaI GCGC 1 cut(s) 525
GluI GCNGC 7 cut(s) 106, 290, 329, 401, 404, 515, 615
HaeIII GGCC 1 cut(s) 187
HgaI GACGC 1 cut(s) 712
HhaI GCGC 1 cut(s) 526
Hin1II CATG 2 cut(s) 257, 700
Hin6I GCGC 1 cut(s) 524
HinP1I GCGC 1 cut(s) 524
HincII GTYRAC 1 cut(s) 541
HindII GTYRAC 1 cut(s) 541
HinfI GANTC 7 cut(s) 22, 144, 229, 409, 568, 609, 740
HphI GGTGA 1 cut(s) 685
Hpy166II GTNNAC 1 cut(s) 541
Hpy188I TCNGA 6 cut(s) 40, 63, 223, 244, 505, 684
Hpy188III TCNNGA 1 cut(s) 604
Hpy8I GTNNAC 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 380, 477, 510
HpyCH4III ACNGT 2 cut(s) 351, 734
HpyCH4V TGCA 7 cut(s) 248, 391, 433, 463, 484, 496, 766
HpyF10VI GCNNNNNNNGC 4 cut(s) 289, 397, 514, 523
HpyF3I CTNAG 5 cut(s) 124, 306, 426, 441, 504
Hsp92II CATG 2 cut(s) 257, 700
HspAI GCGC 1 cut(s) 524
Kzo9I GATC 1 cut(s) 151
LmnI GCTCC 4 cut(s) 269, 277, 475, 721
Lsp1109I GCAGC 6 cut(s) 117, 301, 340, 412, 415, 626
LweI GCATC 5 cut(s) 235, 461, 493, 511, 678
MaeI CTAG 1 cut(s) 772
MaeIII GTNAC 2 cut(s) 173, 351
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 3 cut(s) 429, 643, 697
MfeI CAATTG 2 cut(s) 233, 509
MhlI GDGCHC 1 cut(s) 274
MluCI AATT 6 cut(s) 54, 116, 233, 301, 509, 534
MlyI GAGTC 2 cut(s) 138, 238
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 8 cut(s) 194, 198, 201, 307, 377, 558, 619, 658
MroXI GAANNNNTTC 1 cut(s) 55
MseI TTAA 2 cut(s) 9, 161
MspR9I CCNGG 2 cut(s) 656, 758
MunI CAATTG 2 cut(s) 233, 509
MvaI CCWGG 2 cut(s) 656, 758
MvnI CGCG 1 cut(s) 526
MwoI GCNNNNNNNGC 4 cut(s) 289, 397, 514, 523
NcoI CCATGG 1 cut(s) 696
NdeII GATC 1 cut(s) 151
NlaIII CATG 2 cut(s) 257, 700
NlaIV GGNNCC 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 602
PdmI GAANNNNTTC 1 cut(s) 55
PfeI GAWTC 5 cut(s) 22, 409, 568, 609, 740
PflFI GACNNNGTC 1 cut(s) 85
PkrI GCNGC 7 cut(s) 107, 291, 330, 402, 405, 516, 616
PleI GAGTC 2 cut(s) 138, 237
PpsI GAGTC 2 cut(s) 138, 237
Psp124BI GAGCTC 1 cut(s) 274
Psp6I CCWGG 2 cut(s) 654, 756
PspGI CCWGG 2 cut(s) 654, 756
PspN4I GGNNCC 1 cut(s) 477
PspPI GGNCC 1 cut(s) 203
PstNI CAGNNNCTG 1 cut(s) 455
PsyI GACNNNGTC 1 cut(s) 85
RsaI GTAC 2 cut(s) 314, 341
RsaNI GTAC 2 cut(s) 313, 340
SacI GAGCTC 1 cut(s) 274
SaqAI TTAA 2 cut(s) 9, 161
SatI GCNGC 7 cut(s) 106, 290, 329, 401, 404, 515, 615
Sau3AI GATC 1 cut(s) 151
Sau96I GGNCC 1 cut(s) 203
ScaI AGTACT 1 cut(s) 314
SchI GAGTC 2 cut(s) 138, 238
ScrFI CCNGG 2 cut(s) 656, 758
SduI GDGCHC 1 cut(s) 274
SetI ASST 7 cut(s) 110, 199, 274, 299, 469, 502, 718
SfaNI GCATC 5 cut(s) 235, 461, 493, 511, 678
Sfr274I CTCGAG 1 cut(s) 602
SinI GGWCC 1 cut(s) 203
SlaI CTCGAG 1 cut(s) 602
SmlI CTYRAG 4 cut(s) 192, 602, 623, 649
SmoI CTYRAG 4 cut(s) 192, 602, 623, 649
Sse9I AATT 6 cut(s) 54, 116, 233, 301, 509, 534
SsiI CCGC 2 cut(s) 515, 659
SspMI CTAG 1 cut(s) 772
SstI GAGCTC 1 cut(s) 274
StyD4I CCNGG 2 cut(s) 654, 756
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 2 cut(s) 351, 734
TaqI TCGA 1 cut(s) 603
TasI AATT 6 cut(s) 54, 116, 233, 301, 509, 534
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 517
TfiI GAWTC 5 cut(s) 22, 409, 568, 609, 740
Tru1I TTAA 2 cut(s) 9, 161
Tru9I TTAA 2 cut(s) 9, 161
TseI GCWGC 6 cut(s) 105, 289, 328, 400, 403, 614
TspDTI ATGAA 2 cut(s) 60, 179
TspGWI ACGGA 1 cut(s) 561
Tth111I GACNNNGTC 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 203
XagI CCTNNNNNAGG 1 cut(s) 192
XapI RAATTY 2 cut(s) 54, 301
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XhoI CTCGAG 1 cut(s) 602
XmnI GAANNNNTTC 1 cut(s) 55
XspI CTAG 1 cut(s) 772
ZrmI AGTACT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.