Rh5CG545000

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000315
Physical Location & Seq
Forward (+)
76053489 .. 76055488
2000 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG545000.1

Sequence Viewer

Length: 1341 bp
ATGGTTAGTACTTCTATTATTGATCAAGAGTTTTCAGTGAAGCTTTATCCTTCTGTTGCTTCAACGACTGGGAATACAAATGGAGCTGATTGGCAAGAGGAGGGCTATCAAAAGATCAAAGTCATGAAGGAGATGTACTACCCTGAACTAAGTGAAATGTACCAAAAACTTGCTACTAAACTTCATCAGCACGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAATTTCCAAAACTAGCATAACACCTGGTTTGAAGGAGAAGTTGGGTTTATACGAGAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTCCCCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACTCAAGTGCAATCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCTGGGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACTGCACTGAACTCACTGCAGCAGGTCCCTGCGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCACAGAACGGGATTAACATGCTGCAGAGCAACATTAATCCCCTTCAGTCAAATTCTGGTATGCTTCATGGCCAACATCTGAAACAGCAGGAACAGAAATTGTATCAGAATCAACTCAAGCAACAATATCAACAACGACAGATGCAGCAGCAGCAGTTGATGCAGAAGCAGCAGATATTTCAACAACAACAACAGCAAGAGCTACAGCAGCTGCTGCCTGGACAGTTGGAACCCCAGCTTCACCAAATCAGTGTTGTTAATGACATGAACATGAATCACTTGTCATCATCAAAGAGTGGGAGTAATATGCTGCAGGAAAGCACTGATTCCCTCAAGTCAAACTCTGGTATGCTTGATAACCAGCGTCAGAAGCAAGAAACTTCCTCTGTGAACCTCAAGCAACTGTATCAACAGCTACAGATGCAGCAGCAATGGATGCAGAAACTGCAGATGCTTCAAGAAAAGCAACAGCAGCAGATACAGCAGCTACAGCAGCAAGCAAAGCAGCGGCTGCATTTTATTTATTTCATCTGTCTCATCTTTCTCATTATTCTATACGTTGCTGCTGTTTTCGGGAAAGGCTATTGGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

446

Amino Acids

51.02

Weight (kDa)

9.29

Isoelectric Point (pI)

60.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 667
AciI CCGC 4 cut(s) 395, 416, 686, 1255
AclWI GGATC 2 cut(s) 635, 648
AcoI YGGCCR 1 cut(s) 817
AcsI RAATTY 4 cut(s) 282, 349, 562, 799
AcuI CTGAAG 2 cut(s) 257, 776
AfaI GTAC 3 cut(s) 10, 137, 161
AfiI CCNNNNNNNGG 1 cut(s) 650
AgsI TTSAA 5 cut(s) 63, 313, 591, 929, 1205
AjnI CCWGG 5 cut(s) 304, 371, 397, 643, 964
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
Alw21I GWGCWC 1 cut(s) 718
Alw26I GTCTC 1 cut(s) 1286
AlwI GGATC 2 cut(s) 635, 648
AlwNI CAGNNNCTG 4 cut(s) 958, 961, 1192, 1258
AoxI GGCC 1 cut(s) 817
ApoI RAATTY 4 cut(s) 282, 349, 562, 799
AseI ATTAAT 2 cut(s) 357, 783
AspLEI GCGC 1 cut(s) 264
AspS9I GGNCC 1 cut(s) 679
AsuHPI GGTGA 1 cut(s) 980
AvaII GGWCC 1 cut(s) 679
BalI TGGCCA 1 cut(s) 819
BamHI GGATCC 1 cut(s) 640
BanII GRGCYC 1 cut(s) 692
BarI GAAGNNNNNNTAC 2 cut(s) 119, 151
BbsI GAAGAC 1 cut(s) 564
Bbv12I GWGCWC 1 cut(s) 718
BciT130I CCWGG 5 cut(s) 306, 373, 399, 645, 966
BclI TGATCA 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 1286
BfaI CTAG 1 cut(s) 294
BfmI CTRYAG 7 cut(s) 671, 770, 950, 1058, 1163, 1193, 1235
BfoI RGCGCY 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 667
BglI GCCNNNNNGGC 1 cut(s) 400
BmcAI AGTACT 1 cut(s) 10
Bme1390I CCNGG 5 cut(s) 306, 373, 399, 645, 966
Bme18I GGWCC 1 cut(s) 679
BmgT120I GGNCC 1 cut(s) 679
BmiI GGNNCC 3 cut(s) 642, 681, 978
BmrFI CCNGG 5 cut(s) 306, 373, 399, 645, 966
BmrI ACTGGG 1 cut(s) 78
BmsI GCATC 5 cut(s) 879, 897, 1158, 1173, 1188
BmuI ACTGGG 1 cut(s) 78
BoxI GACNNNNGTC 1 cut(s) 707
BpiI GAAGAC 1 cut(s) 564
BpmI CTGGAG 1 cut(s) 278
BpuEI CTTGAG 5 cut(s) 248, 441, 848, 1064, 1127
BsaJI CCNNGG 2 cut(s) 371, 398
Bsc4I CCNNNNNNNGG 1 cut(s) 650
Bse1I ACTGG 1 cut(s) 73
Bse3DI GCAATG 1 cut(s) 1184
BseBI CCWGG 5 cut(s) 306, 373, 399, 645, 966
BseDI CCNNGG 2 cut(s) 371, 398
BseGI GGATG 1 cut(s) 1188
BseLI CCNNNNNNNGG 1 cut(s) 650
BseMI GCAATG 1 cut(s) 1184
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 1 cut(s) 113
BseYI CCCAGC 1 cut(s) 981
BsgI GTGCAG 1 cut(s) 642
BshFI GGCC 1 cut(s) 819
BsiHKAI GWGCWC 1 cut(s) 718
BslFI GGGAC 1 cut(s) 665
BslI CCNNNNNNNGG 1 cut(s) 650
BsmAI GTCTC 1 cut(s) 1286
BsmFI GGGAC 1 cut(s) 665
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 1 cut(s) 819
Bsp1286I GDGCHC 2 cut(s) 692, 718
Bsp143I GATC 4 cut(s) 22, 114, 220, 640
BspACI CCGC 4 cut(s) 395, 416, 686, 1255
BspANI GGCC 1 cut(s) 819
BspHI TCATGA 2 cut(s) 123, 469
BspLI GGNNCC 3 cut(s) 642, 681, 978
BspMAI CTGCAG 4 cut(s) 675, 774, 1062, 1197
BspMI ACCTGC 1 cut(s) 667
BspPI GGATC 2 cut(s) 635, 648
BsrDI GCAATG 1 cut(s) 1184
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 371, 398
BssMI GATC 4 cut(s) 22, 114, 220, 640
Bst2UI CCWGG 5 cut(s) 306, 373, 399, 645, 966
Bst4CI ACNGT 3 cut(s) 274, 972, 1152
BstAPI GCANNNNNTGC 6 cut(s) 430, 907, 961, 1183, 1192, 1258
BstC8I GCNNGC 3 cut(s) 554, 688, 1245
BstDEI CTNAG 1 cut(s) 149
BstF5I GGATG 1 cut(s) 1188
BstH2I RGCGCY 1 cut(s) 265
BstHHI GCGC 1 cut(s) 264
BstKTI GATC 4 cut(s) 25, 117, 223, 643
BstMAI GTCTC 1 cut(s) 1286
BstMBI GATC 4 cut(s) 22, 114, 220, 640
BstNI CCWGG 5 cut(s) 306, 373, 399, 645, 966
BstNSI RCATGY 1 cut(s) 769
BstPAI GACNNNNGTC 1 cut(s) 707
BstSCI CCNGG 5 cut(s) 304, 371, 397, 643, 964
BstSFI CTRYAG 7 cut(s) 671, 770, 950, 1058, 1163, 1193, 1235
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 1 cut(s) 819
BtsCI GGATG 1 cut(s) 1188
BtsI GCAGTG 1 cut(s) 668
BtsIMutI CAGTG 6 cut(s) 42, 659, 668, 718, 1003, 1068
BveI ACCTGC 1 cut(s) 667
Cac8I GCNNGC 3 cut(s) 554, 688, 1245
CaiI CAGNNNCTG 4 cut(s) 958, 961, 1192, 1258
CciI TCATGA 2 cut(s) 123, 469
CfoI GCGC 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 679
CseI GACGC 1 cut(s) 1100
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 3 cut(s) 9, 136, 160
CviQI GTAC 3 cut(s) 9, 136, 160
DdeI CTNAG 1 cut(s) 149
DpnI GATC 4 cut(s) 24, 116, 222, 642
DpnII GATC 4 cut(s) 22, 114, 220, 640
EaeI YGGCCR 1 cut(s) 817
Eco24I GRGCYC 1 cut(s) 692
Eco47I GGWCC 1 cut(s) 679
Eco57I CTGAAG 2 cut(s) 257, 776
EcoO109I RGGNCCY 1 cut(s) 679
EcoRII CCWGG 5 cut(s) 304, 371, 397, 643, 964
EcoT38I GRGCYC 1 cut(s) 692
FaqI GGGAC 1 cut(s) 665
FauI CCCGC 1 cut(s) 679
FauNDI CATATG 1 cut(s) 420
FbaI TGATCA 1 cut(s) 22
FokI GGATG 1 cut(s) 1195
FriOI GRGCYC 1 cut(s) 692
FspBI CTAG 1 cut(s) 294
GlaI GCGC 1 cut(s) 263
GsaI CCCAGC 1 cut(s) 985
GsuI CTGGAG 1 cut(s) 278
HaeII RGCGCY 1 cut(s) 265
HaeIII GGCC 1 cut(s) 819
HgaI GACGC 1 cut(s) 1100
HhaI GCGC 1 cut(s) 264
Hin6I GCGC 1 cut(s) 262
HinP1I GCGC 1 cut(s) 262
HindIII AAGCTT 1 cut(s) 41
HinfI GANTC 6 cut(s) 194, 613, 703, 856, 1021, 1073
HphI GGTGA 1 cut(s) 980
Hpy166II GTNNAC 1 cut(s) 1138
Hpy188I TCNGA 5 cut(s) 220, 480, 828, 855, 1116
Hpy188III TCNNGA 5 cut(s) 26, 124, 470, 1205, 1321
Hpy8I GTNNAC 1 cut(s) 1138
HpyAV CCTTC 5 cut(s) 60, 121, 209, 307, 800
HpyCH4III ACNGT 3 cut(s) 274, 972, 1152
HpyCH4IV ACGT 1 cut(s) 1305
HpyF3I CTNAG 1 cut(s) 149
HpySE526I ACGT 1 cut(s) 1305
HspAI GCGC 1 cut(s) 262
Ksp22I TGATCA 1 cut(s) 22
Kzo9I GATC 4 cut(s) 22, 114, 220, 640
LmnI GCTCC 4 cut(s) 83, 259, 411, 721
LweI GCATC 5 cut(s) 879, 897, 1158, 1173, 1188
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 1 cut(s) 294
MaeII ACGT 1 cut(s) 1305
MaeIII GTNAC 1 cut(s) 438
MalI GATC 4 cut(s) 24, 116, 222, 642
MboI GATC 4 cut(s) 22, 114, 220, 640
MboII GAAGA 3 cut(s) 250, 378, 564
MfeI CAATTG 1 cut(s) 491
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 692, 718
MlsI TGGCCA 1 cut(s) 819
MluCI AATT 6 cut(s) 282, 349, 491, 562, 799, 845
MluNI TGGCCA 1 cut(s) 819
MlyI GAGTC 2 cut(s) 188, 697
MmeI TCCRAC 2 cut(s) 721, 954
MnlI CCTC 5 cut(s) 91, 94, 1088, 1141, 1151
Mox20I TGGCCA 1 cut(s) 819
MscI TGGCCA 1 cut(s) 819
MseI TTAA 6 cut(s) 246, 357, 762, 783, 1005, 1339
MslI CAYNNNNRTG 1 cut(s) 1016
Msp20I TGGCCA 1 cut(s) 819
MspA1I CMGCKG 2 cut(s) 958, 1255
MspR9I CCNGG 5 cut(s) 306, 373, 399, 645, 966
MunI CAATTG 1 cut(s) 491
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 5 cut(s) 306, 373, 399, 645, 966
NdeI CATATG 1 cut(s) 420
NdeII GATC 4 cut(s) 22, 114, 220, 640
NlaIV GGNNCC 3 cut(s) 642, 681, 978
NmuCI GTSAC 1 cut(s) 438
NspI RCATGY 1 cut(s) 769
PagI TCATGA 2 cut(s) 123, 469
PctI GAATGC 1 cut(s) 742
PfeI GAWTC 4 cut(s) 613, 856, 1021, 1073
PfoI TCCNGGA 1 cut(s) 643
PleI GAGTC 2 cut(s) 188, 697
PpsI GAGTC 2 cut(s) 188, 697
PpuMI RGGWCCY 1 cut(s) 679
PshAI GACNNNNGTC 1 cut(s) 707
PshBI ATTAAT 2 cut(s) 357, 783
Psp5II RGGWCCY 1 cut(s) 679
Psp6I CCWGG 5 cut(s) 304, 371, 397, 643, 964
PspFI CCCAGC 1 cut(s) 981
PspGI CCWGG 5 cut(s) 304, 371, 397, 643, 964
PspN4I GGNNCC 3 cut(s) 642, 681, 978
PspPI GGNCC 1 cut(s) 679
PspPPI RGGWCCY 1 cut(s) 679
PstI CTGCAG 4 cut(s) 675, 774, 1062, 1197
PstNI CAGNNNCTG 4 cut(s) 958, 961, 1192, 1258
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 1 cut(s) 958
RsaI GTAC 3 cut(s) 10, 137, 161
RsaNI GTAC 3 cut(s) 9, 136, 160
RseI CAYNNNNRTG 1 cut(s) 1016
SaqAI TTAA 6 cut(s) 246, 357, 762, 783, 1005, 1339
Sau3AI GATC 4 cut(s) 22, 114, 220, 640
Sau96I GGNCC 1 cut(s) 679
ScaI AGTACT 1 cut(s) 10
SchI GAGTC 2 cut(s) 188, 697
ScrFI CCNGG 5 cut(s) 306, 373, 399, 645, 966
SduI GDGCHC 2 cut(s) 692, 718
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 5 cut(s) 879, 897, 1158, 1173, 1188
SfcI CTRYAG 7 cut(s) 671, 770, 950, 1058, 1163, 1193, 1235
SinI GGWCC 1 cut(s) 679
SmiMI CAYNNNNRTG 1 cut(s) 1016
SmlI CTYRAG 5 cut(s) 227, 456, 863, 1079, 1142
SmoI CTYRAG 5 cut(s) 227, 456, 863, 1079, 1142
Sse9I AATT 6 cut(s) 282, 349, 491, 562, 799, 845
SsiI CCGC 4 cut(s) 395, 416, 686, 1255
SspMI CTAG 1 cut(s) 294
StyD4I CCNGG 5 cut(s) 304, 371, 397, 643, 964
TaaI ACNGT 3 cut(s) 274, 972, 1152
TaiI ACGT 1 cut(s) 1308
TasI AATT 6 cut(s) 282, 349, 491, 562, 799, 845
TatI WGTACW 2 cut(s) 8, 135
TauI GCSGC 2 cut(s) 397, 1258
TfiI GAWTC 4 cut(s) 613, 856, 1021, 1073
Tru1I TTAA 6 cut(s) 246, 357, 762, 783, 1005, 1339
Tru9I TTAA 6 cut(s) 246, 357, 762, 783, 1005, 1339
TscAI CASTG 6 cut(s) 42, 666, 675, 718, 1003, 1075
TseFI GTSAC 1 cut(s) 438
Tsp45I GTSAC 1 cut(s) 438
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 6 cut(s) 42, 666, 675, 718, 1003, 1075
VpaK11BI GGWCC 1 cut(s) 679
VspI ATTAAT 2 cut(s) 357, 783
XapI RAATTY 4 cut(s) 282, 349, 562, 799
XceI RCATGY 1 cut(s) 769
XspI CTAG 1 cut(s) 294
ZrmI AGTACT 1 cut(s) 10
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.