Rh5AG499600

KIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000315
Physical Location & Seq
Forward (+)
84634256 .. 84638365
4110 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG499600.1

Sequence Viewer

Length: 993 bp
ATGGATCTATTGAAGAGGCATCTTCCTTTCTCCAGTCAAGAGGGATTGGACGAACTCAAGAAAATTGCTGTTACGTTTGAGGAAAAGATTTATACTGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTGAAGATGCTCACTATGGAGACCAGGTTCATGAAGTACAACGATGGGAGTCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAAACAATTGCAGGCGGATTCGCGGCAAAGAATTGTCAATAAGCTTATGGATCTATTGAAGAGGCATCTTCCTTTCTCCAGTCAAGAGGGATTGCACGAACTCGAGAAAATTGCTGTTACATTTGAGGAAAAGATTTATACTGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTGAAGATGCTCACTATGGAGACCAGTCCCCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGACTGGAGGAGCCAATTGCAGGCGGATTTGCGGCAAAGAATTGTCAATAAGCTGATGGATACATTGAAGAGGCATCTTCCTTTCTCCGGTCAAGAGGGATTGGACGAACTCAAGAAAATTGCTGTTAGATTTGAGGAAAAGATTTATGGTGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTGAAGATGATCACTATGGAGACCAAGTCTCAGAACACAATGGCCAATTCTTTACAATCCAACTCTGCTGTCACCAATCAAGGACAACTCTTATCAGAGAACATTCAAAGTAACATTCCGCCTGCTGGAGTTCAAAATTCTGCTGATTTATCATCTGCACTAACCCCTACCTCTGGGCCCGGCTTATCATCTGTACTAGCTCCTACCCCGGCACTAAATACGTCTGCCTTACTATCTGCACTACCCCCTGCCCCTGGTAGAAATATGCCTGCCAGCTTATCATCTGCGTTACATTCTCCACAGGACATGAATATGTCTAGTTCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

330

Amino Acids

36.33

Weight (kDa)

7.85

Isoelectric Point (pI)

65.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 1 - 57 1.2e-20 KIX domain
KIX_2 PF16987 74 - 148 3.2e-31 KIX domain
KIX_2 PF16987 158 - 238 1.6e-34 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 253
AciI CCGC 5 cut(s) 245, 253, 497, 505, 782
AclWI GGATC 2 cut(s) 12, 288
AcoI YGGCCR 1 cut(s) 705
AcsI RAATTY 4 cut(s) 129, 405, 657, 799
AcuI CTGAAG 3 cut(s) 158, 434, 686
AfaI GTAC 2 cut(s) 173, 858
AfiI CCNNNNNNNGG 9 cut(s) 194, 197, 446, 449, 493, 560, 788, 836, 917
AgsI TTSAA 5 cut(s) 13, 289, 541, 770, 797
AjnI CCWGG 2 cut(s) 158, 916
AloI GAACNNNNNNTCC 2 cut(s) 146, 178
AluBI AGCT 4 cut(s) 274, 526, 863, 939
AluI AGCT 4 cut(s) 274, 526, 863, 939
Alw26I GTCTC 5 cut(s) 149, 425, 468, 677, 696
AlwI GGATC 2 cut(s) 12, 288
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 2 cut(s) 705, 839
ApaI GGGCCC 1 cut(s) 843
ApeKI GCWGC 3 cut(s) 98, 374, 626
ApoI RAATTY 4 cut(s) 129, 405, 657, 799
AspS9I GGNCC 2 cut(s) 839, 840
AsuC2I CCSGG 2 cut(s) 843, 872
AsuHPI GGTGA 1 cut(s) 727
AvaI CYCGRG 1 cut(s) 332
BaeGI GKGCMC 1 cut(s) 843
BalI TGGCCA 1 cut(s) 707
BanII GRGCYC 1 cut(s) 843
BbvI GCAGC 3 cut(s) 85, 361, 613
BccI CCATC 2 cut(s) 173, 523
BcgI CGANNNNNNTGC 2 cut(s) 323, 357
BciT130I CCWGG 2 cut(s) 160, 918
BciVI GTATCC 1 cut(s) 526
BclI TGATCA 1 cut(s) 672
BcnI CCSGG 2 cut(s) 843, 872
BcoDI GTCTC 5 cut(s) 149, 425, 468, 677, 696
BfaI CTAG 2 cut(s) 860, 981
BfuI GTATCC 1 cut(s) 526
BisI GCNGC 5 cut(s) 99, 254, 375, 506, 627
BlsI GCNGC 5 cut(s) 100, 255, 376, 507, 628
Bme1390I CCNGG 4 cut(s) 160, 843, 872, 918
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 839, 840
BmiI GGNNCC 2 cut(s) 485, 841
BmrFI CCNGG 4 cut(s) 160, 843, 872, 918
BmsI GCATC 7 cut(s) 28, 132, 205, 304, 408, 457, 556
BplI GAGNNNNNCTC 2 cut(s) 175, 207
BpmI CTGGAG 4 cut(s) 16, 292, 499, 810
BpuEI CTTGAG 4 cut(s) 41, 177, 429, 569
BpuMI CCSGG 2 cut(s) 843, 872
BsaI GGTCTC 3 cut(s) 149, 425, 677
BsaJI CCNNGG 2 cut(s) 870, 916
BsaWI WCCGGW 1 cut(s) 560
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 674, 704
Bsc4I CCNNNNNNNGG 9 cut(s) 194, 197, 446, 449, 493, 560, 788, 836, 917
Bse1I ACTGG 4 cut(s) 33, 309, 435, 482
Bse3DI GCAATG 2 cut(s) 216, 468
BseBI CCWGG 2 cut(s) 160, 918
BseDI CCNNGG 2 cut(s) 870, 916
BseGI GGATG 2 cut(s) 220, 472
BseLI CCNNNNNNNGG 9 cut(s) 194, 197, 446, 449, 493, 560, 788, 836, 917
BseMI GCAATG 2 cut(s) 216, 468
BseMII CTCAG 1 cut(s) 707
BseNI ACTGG 4 cut(s) 33, 309, 435, 482
BseRI GAGGAG 2 cut(s) 180, 496
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 3 cut(s) 85, 361, 613
BsgI GTGCAG 2 cut(s) 804, 885
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 2 cut(s) 707, 841
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 3 cut(s) 561, 843, 872
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 9 cut(s) 194, 197, 446, 449, 493, 560, 788, 836, 917
BsmAI GTCTC 5 cut(s) 149, 425, 468, 677, 696
BsmFI GGGAC 1 cut(s) 423
BsnI GGCC 2 cut(s) 707, 841
Bso31I GGTCTC 3 cut(s) 149, 425, 677
BsoBI CYCGRG 1 cut(s) 332
Bsp120I GGGCCC 1 cut(s) 839
Bsp1286I GDGCHC 1 cut(s) 843
Bsp143I GATC 3 cut(s) 4, 280, 672
BspACI CCGC 5 cut(s) 245, 253, 497, 505, 782
BspANI GGCC 2 cut(s) 707, 841
BspCNI CTCAG 1 cut(s) 706
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 165
BspLI GGNNCC 2 cut(s) 485, 841
BspPI GGATC 2 cut(s) 12, 288
BspTNI GGTCTC 3 cut(s) 149, 425, 677
BsrDI GCAATG 2 cut(s) 216, 468
BsrI ACTGG 4 cut(s) 33, 309, 435, 482
BssECI CCNNGG 2 cut(s) 870, 916
BssMI GATC 3 cut(s) 4, 280, 672
Bst2UI CCWGG 2 cut(s) 160, 918
Bst6I CTCTTC 3 cut(s) 8, 284, 536
BstAPI GCANNNNNTGC 2 cut(s) 215, 467
BstC8I GCNNGC 7 cut(s) 207, 243, 459, 495, 786, 933, 937
BstDEI CTNAG 1 cut(s) 693
BstENI CCTNNNNNAGG 1 cut(s) 192
BstF5I GGATG 2 cut(s) 220, 472
BstFNI CGCG 1 cut(s) 253
BstKTI GATC 3 cut(s) 7, 283, 675
BstMAI GTCTC 5 cut(s) 149, 425, 468, 677, 696
BstMBI GATC 3 cut(s) 4, 280, 672
BstMWI GCNNNNNNNGC 2 cut(s) 215, 467
BstNI CCWGG 2 cut(s) 160, 918
BstSCI CCNGG 4 cut(s) 158, 841, 870, 916
BstSLI GKGCMC 1 cut(s) 843
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 3 cut(s) 85, 361, 613
BstX2I RGATCY 2 cut(s) 4, 280
BstYI RGATCY 2 cut(s) 4, 280
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 2 cut(s) 707, 841
BtsCI GGATG 2 cut(s) 220, 472
Cac8I GCNNGC 7 cut(s) 207, 243, 459, 495, 786, 933, 937
CciI TCATGA 1 cut(s) 165
Cfr13I GGNCC 2 cut(s) 839, 840
CsiI ACCWGGT 1 cut(s) 158
Csp6I GTAC 2 cut(s) 172, 857
CviAII CATG 2 cut(s) 166, 970
CviQI GTAC 2 cut(s) 172, 857
DdeI CTNAG 1 cut(s) 693
DpnI GATC 3 cut(s) 6, 282, 674
DpnII GATC 3 cut(s) 4, 280, 672
EaeI YGGCCR 1 cut(s) 705
Eam1104I CTCTTC 3 cut(s) 8, 284, 536
EarI CTCTTC 3 cut(s) 8, 284, 536
EciI GGCGGA 3 cut(s) 260, 512, 771
Eco24I GRGCYC 1 cut(s) 843
Eco31I GGTCTC 3 cut(s) 149, 425, 677
Eco57I CTGAAG 3 cut(s) 158, 434, 686
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 192
EcoRII CCWGG 2 cut(s) 158, 916
EcoT38I GRGCYC 1 cut(s) 843
FaeI CATG 2 cut(s) 169, 973
FaqI GGGAC 1 cut(s) 423
FatI CATG 2 cut(s) 165, 969
FbaI TGATCA 1 cut(s) 672
Fnu4HI GCNGC 5 cut(s) 99, 254, 375, 506, 627
FokI GGATG 2 cut(s) 227, 479
FriOI GRGCYC 1 cut(s) 843
Fsp4HI GCNGC 5 cut(s) 99, 254, 375, 506, 627
FspBI CTAG 2 cut(s) 860, 981
GluI GCNGC 5 cut(s) 99, 254, 375, 506, 627
GsuI CTGGAG 4 cut(s) 16, 292, 499, 810
HaeIII GGCC 2 cut(s) 707, 841
HapII CCGG 3 cut(s) 561, 843, 872
Hin1II CATG 2 cut(s) 169, 973
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 184, 248
HpaII CCGG 3 cut(s) 561, 843, 872
HphI GGTGA 1 cut(s) 727
Hpy188I TCNGA 8 cut(s) 115, 138, 391, 414, 643, 666, 696, 760
Hpy188III TCNNGA 7 cut(s) 38, 58, 166, 314, 334, 566, 586
HpyCH4IV ACGT 2 cut(s) 74, 884
HpyCH4V TGCA 9 cut(s) 209, 218, 241, 325, 461, 470, 493, 821, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 215, 467
HpyF3I CTNAG 1 cut(s) 693
HpySE526I ACGT 2 cut(s) 74, 884
Hsp92II CATG 2 cut(s) 169, 973
Ksp22I TGATCA 1 cut(s) 672
Kzo9I GATC 3 cut(s) 4, 280, 672
LmnI GCTCC 2 cut(s) 483, 868
Lsp1109I GCAGC 3 cut(s) 85, 361, 613
LweI GCATC 7 cut(s) 28, 132, 205, 304, 408, 457, 556
MabI ACCWGGT 1 cut(s) 158
MaeI CTAG 2 cut(s) 860, 981
MaeII ACGT 2 cut(s) 74, 884
MaeIII GTNAC 5 cut(s) 70, 346, 733, 773, 951
MalI GATC 3 cut(s) 6, 282, 674
MboI GATC 3 cut(s) 4, 280, 672
MboII GAAGA 9 cut(s) 14, 25, 151, 290, 301, 427, 542, 553, 679
MfeI CAATTG 2 cut(s) 236, 488
MflI RGATCY 2 cut(s) 4, 280
MhlI GDGCHC 1 cut(s) 843
MlsI TGGCCA 1 cut(s) 707
MluNI TGGCCA 1 cut(s) 707
MlyI GAGTC 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 747
Mox20I TGGCCA 1 cut(s) 707
MscI TGGCCA 1 cut(s) 707
MslI CAYNNNNRTG 1 cut(s) 974
Msp20I TGGCCA 1 cut(s) 707
MspI CCGG 3 cut(s) 561, 843, 872
MspR9I CCNGG 4 cut(s) 160, 843, 872, 918
MunI CAATTG 2 cut(s) 236, 488
MvaI CCWGG 2 cut(s) 160, 918
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 2 cut(s) 215, 467
NciI CCSGG 2 cut(s) 843, 872
NdeII GATC 3 cut(s) 4, 280, 672
NlaIII CATG 2 cut(s) 169, 973
NlaIV GGNNCC 2 cut(s) 485, 841
NmuCI GTSAC 1 cut(s) 733
PaeR7I CTCGAG 1 cut(s) 332
PagI TCATGA 1 cut(s) 165
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 688
PkrI GCNGC 5 cut(s) 100, 255, 376, 507, 628
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 158, 916
PspGI CCWGG 2 cut(s) 158, 916
PspN4I GGNNCC 2 cut(s) 485, 841
PspOMI GGGCCC 1 cut(s) 839
PspPI GGNCC 2 cut(s) 839, 840
PsuI RGATCY 2 cut(s) 4, 280
PsyI GACNNNGTC 1 cut(s) 688
RsaI GTAC 2 cut(s) 173, 858
RsaNI GTAC 2 cut(s) 172, 857
RseI CAYNNNNRTG 1 cut(s) 974
SatI GCNGC 5 cut(s) 99, 254, 375, 506, 627
Sau3AI GATC 3 cut(s) 4, 280, 672
Sau96I GGNCC 2 cut(s) 839, 840
SchI GAGTC 1 cut(s) 193
ScrFI CCNGG 4 cut(s) 160, 843, 872, 918
SduI GDGCHC 1 cut(s) 843
SexAI ACCWGGT 1 cut(s) 158
SfaNI GCATC 7 cut(s) 28, 132, 205, 304, 408, 457, 556
Sfr274I CTCGAG 1 cut(s) 332
SlaI CTCGAG 1 cut(s) 332
SmiMI CAYNNNNRTG 1 cut(s) 974
SmlI CTYRAG 5 cut(s) 56, 192, 332, 444, 584
SmoI CTYRAG 5 cut(s) 56, 192, 332, 444, 584
SsiI CCGC 5 cut(s) 245, 253, 497, 505, 782
SspMI CTAG 2 cut(s) 860, 981
StyD4I CCNGG 4 cut(s) 158, 841, 870, 916
TaiI ACGT 2 cut(s) 77, 887
TaqI TCGA 1 cut(s) 333
TatI WGTACW 2 cut(s) 171, 856
TauI GCSGC 2 cut(s) 256, 508
TfiI GAWTC 1 cut(s) 248
TseFI GTSAC 1 cut(s) 733
TseI GCWGC 3 cut(s) 98, 374, 626
Tsp45I GTSAC 1 cut(s) 733
TspDTI ATGAA 4 cut(s) 154, 182, 975, 986
TspGWI ACGGA 3 cut(s) 139, 415, 667
Tth111I GACNNNGTC 1 cut(s) 688
XagI CCTNNNNNAGG 1 cut(s) 192
XapI RAATTY 4 cut(s) 129, 405, 657, 799
XhoI CTCGAG 1 cut(s) 332
XspI CTAG 2 cut(s) 860, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.