pycom03g02570

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000315
Physical Location & Seq
Reverse (-)
1961599 .. 1963038
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02570.1

Sequence Viewer

Length: 336 bp
ATGGATCTCAATAACCAGAGACCTGCTCAAGGTGGAGAACCTTCAATGGAGGCAGGTGATTGGAGAAGCCATTTGCAGCAAGATTCACGACGCAGAATTGTTCACAAGATAACGGAAACGTTGAAGAGGTACCTTCCCTTCGAGGGGGAGGAGGGATTACGTGCACTCGAGAACATTGCTGTAAGGTTTGAGGAAAAGATATATGTTGCTGCCTCAAGCCAGTCTGATTACCTACGGAAAATTTCTCTCAAGATGCTCACCATGGAGACCAAGTCTCAGAACGCAGTGTCCGCGACACCCCCCTCCAACCTGAAGCGCCATCGCGTCCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

112

Amino Acids

12.75

Weight (kDa)

9.62

Isoelectric Point (pI)

79.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 97 3.2e-31 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 2 cut(s) 31, 44
Acc65I GGTACC 1 cut(s) 129
AccB1I GGYRCC 1 cut(s) 129
AccII CGCG 2 cut(s) 293, 324
AciI CCGC 1 cut(s) 291
AclI AACGTT 1 cut(s) 119
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 332
AfaI GTAC 1 cut(s) 131
AfiI CCNNNNNNNGG 3 cut(s) 29, 143, 144
AgsI TTSAA 2 cut(s) 45, 124
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
Alw21I GWGCWC 1 cut(s) 166
Alw26I GTCTC 3 cut(s) 13, 260, 279
Alw44I GTGCAC 1 cut(s) 162
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 167
ApaLI GTGCAC 1 cut(s) 162
ApeKI GCWGC 2 cut(s) 76, 209
ApoI RAATTY 1 cut(s) 240
Asp718I GGTACC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 318
AsuHPI GGTGA 2 cut(s) 68, 250
AvaI CYCGRG 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 166
BanI GGYRCC 1 cut(s) 129
BarI GAAGNNNNNNTAC 2 cut(s) 122, 154
Bbv12I GWGCWC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 88, 196
BccI CCATC 1 cut(s) 327
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BcoDI GTCTC 3 cut(s) 13, 260, 279
BfoI RGCGCY 1 cut(s) 319
BfuAI ACCTGC 2 cut(s) 31, 44
BisI GCNGC 2 cut(s) 77, 210
BlsI GCNGC 2 cut(s) 78, 211
BmeT110I CYCGRG 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 131
BmsI GCATC 1 cut(s) 243
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 3 cut(s) 12, 199, 233
BsaAI YACGTR 1 cut(s) 161
BsaI GGTCTC 2 cut(s) 13, 260
BsaJI CCNNGG 1 cut(s) 261
BsaXI ACNNNNNCTCC 2 cut(s) 257, 287
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 143, 144
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 174
BseDI CCNNGG 1 cut(s) 261
BseLI CCNNNNNNNGG 3 cut(s) 29, 143, 144
BseMI GCAATG 1 cut(s) 174
BseMII CTCAG 1 cut(s) 290
BseNI ACTGG 1 cut(s) 220
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 166
BseXI GCAGC 2 cut(s) 88, 196
Bsh1236I CGCG 2 cut(s) 293, 324
BshNI GGYRCC 1 cut(s) 129
BsiHKAI GWGCWC 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 167
BslI CCNNNNNNNGG 3 cut(s) 29, 143, 144
BsmAI GTCTC 3 cut(s) 13, 260, 279
Bso31I GGTCTC 2 cut(s) 13, 260
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 166
Bsp143I GATC 1 cut(s) 4
Bsp19I CCATGG 1 cut(s) 261
BspACI CCGC 1 cut(s) 291
BspCNI CTCAG 1 cut(s) 289
BspFNI CGCG 2 cut(s) 293, 324
BspLI GGNNCC 1 cut(s) 131
BspMI ACCTGC 2 cut(s) 31, 44
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 129
BspTNI GGTCTC 2 cut(s) 13, 260
BsrDI GCAATG 1 cut(s) 174
BsrI ACTGG 1 cut(s) 220
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 1 cut(s) 4
BssT1I CCWWGG 1 cut(s) 261
Bst6I CTCTTC 1 cut(s) 119
BstBAI YACGTR 1 cut(s) 161
BstDEI CTNAG 1 cut(s) 276
BstDSI CCRYGG 1 cut(s) 261
BstENI CCTNNNNNAGG 1 cut(s) 27
BstFNI CGCG 2 cut(s) 293, 324
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 3 cut(s) 13, 260, 279
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 1 cut(s) 290
BstSLI GKGCMC 1 cut(s) 166
BstUI CGCG 2 cut(s) 293, 324
BstV1I GCAGC 2 cut(s) 88, 196
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtgI CCRYGG 1 cut(s) 261
BtgZI GCGATG 1 cut(s) 305
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 1 cut(s) 291
BveI ACCTGC 2 cut(s) 31, 44
CfoI GCGC 1 cut(s) 318
CseI GACGC 2 cut(s) 99, 313
Csp6I GTAC 1 cut(s) 130
CviAII CATG 1 cut(s) 262
CviJI RGCY 2 cut(s) 69, 219
CviKI_1 RGCY 2 cut(s) 69, 219
CviQI GTAC 1 cut(s) 130
DdeI CTNAG 1 cut(s) 276
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 261
Eco31I GGTCTC 2 cut(s) 13, 260
Eco57I CTGAAG 1 cut(s) 332
Eco88I CYCGRG 1 cut(s) 167
EcoNI CCTNNNNNAGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 261
ErhI CCWWGG 1 cut(s) 261
FaeI CATG 1 cut(s) 265
FaiI YATR 3 cut(s) 202, 204, 263
FatI CATG 1 cut(s) 261
Fnu4HI GCNGC 2 cut(s) 77, 210
Fsp4HI GCNGC 2 cut(s) 77, 210
GlaI GCGC 1 cut(s) 317
GluI GCNGC 2 cut(s) 77, 210
HaeII RGCGCY 1 cut(s) 319
HgaI GACGC 2 cut(s) 99, 313
HhaI GCGC 1 cut(s) 318
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 1 cut(s) 83
HphI GGTGA 2 cut(s) 68, 250
Hpy166II GTNNAC 2 cut(s) 103, 164
Hpy188I TCNGA 2 cut(s) 226, 279
Hpy188III TCNNGA 3 cut(s) 87, 169, 250
Hpy8I GTNNAC 2 cut(s) 103, 164
Hpy99I CGWCG 1 cut(s) 93
HpyAV CCTTC 3 cut(s) 51, 143, 148
HpyCH4IV ACGT 2 cut(s) 119, 160
HpyCH4V TGCA 2 cut(s) 76, 164
HpyF10VI GCNNNNNNNGC 1 cut(s) 290
HpyF3I CTNAG 1 cut(s) 276
HpySE526I ACGT 2 cut(s) 119, 160
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 1 cut(s) 316
KpnI GGTACC 1 cut(s) 133
Kzo9I GATC 1 cut(s) 4
LpnPI CCDG 5 cut(s) 29, 36, 39, 233, 323
Lsp1109I GCAGC 2 cut(s) 88, 196
LweI GCATC 1 cut(s) 243
MaeII ACGT 2 cut(s) 119, 160
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 1 cut(s) 136
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 166
MluCI AATT 2 cut(s) 96, 240
MmeI TCCRAC 1 cut(s) 330
MnlI CCTC 8 cut(s) 43, 120, 136, 142, 145, 184, 223, 313
MvnI CGCG 2 cut(s) 293, 324
MwoI GCNNNNNNNGC 1 cut(s) 290
NcoI CCATGG 1 cut(s) 261
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 1 cut(s) 131
PaeR7I CTCGAG 1 cut(s) 167
PaqCI CACCTGC 1 cut(s) 44
PfeI GAWTC 1 cut(s) 83
PflFI GACNNNGTC 1 cut(s) 271
PkrI GCNGC 2 cut(s) 78, 211
Ppu21I YACGTR 1 cut(s) 161
Psp1406I AACGTT 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 271
RsaI GTAC 1 cut(s) 131
RsaNI GTAC 1 cut(s) 130
SatI GCNGC 2 cut(s) 77, 210
Sau3AI GATC 1 cut(s) 4
SduI GDGCHC 1 cut(s) 166
SfaNI GCATC 1 cut(s) 243
Sfr274I CTCGAG 1 cut(s) 167
SlaI CTCGAG 1 cut(s) 167
SmlI CTYRAG 4 cut(s) 27, 167, 214, 248
SmoI CTYRAG 4 cut(s) 27, 167, 214, 248
Sse9I AATT 2 cut(s) 96, 240
SsiI CCGC 1 cut(s) 291
StyI CCWWGG 1 cut(s) 261
TaiI ACGT 2 cut(s) 122, 163
TaqI TCGA 2 cut(s) 141, 168
TasI AATT 2 cut(s) 96, 240
TfiI GAWTC 1 cut(s) 83
TscAI CASTG 1 cut(s) 291
TseI GCWGC 2 cut(s) 76, 209
TspGWI ACGGA 2 cut(s) 128, 250
TspRI CASTG 1 cut(s) 291
Tth111I GACNNNGTC 1 cut(s) 271
VneI GTGCAC 1 cut(s) 162
XagI CCTNNNNNAGG 1 cut(s) 27
XapI RAATTY 1 cut(s) 240
XhoI CTCGAG 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.