Rh5CG543900

KIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000315
Physical Location & Seq
Forward (+)
75927030 .. 75928741
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG543900.1

Sequence Viewer

Length: 480 bp
ATGTGGAATTATAGAAAAGAAAGTAAAGGTGACCAAGAAGTTAGTAAAGTCCACAGCAAAGTTCTTCTTCATTTGAGTCAGTCAACCTCTCTGTCTTTATCAGCCTCTCTCAAGATTTTGATGGATACCAATATCAACAGTGATCAGAGGCCTCCACAGGGTGGAGAAGCCCCAGATGACACCGGCAATTGGAGGATGTACTTGATGCCAGATTCAAGACAAAGAGTTGTCAACAAGATATTTGAAACGTTGAAGATGCATCTCCCCTTCTCTGGCCAAGAGGGGTTACTTGAACTGAAGAGAATTGGTTTAAGGTTTGAGGAAAAGGTGTATGCTACTGCATCAAGCCAGTCGGATTATCTACGAACTATTGCTCTGAAGATGCTTACCATTGAGGGCAAGGAAGTTGCAACCAGTCCCCCCTCCAACCTGAAACGGCATCGTGTCCTTGCTTTTGAAACAGAGTATCAAGTTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

159

Amino Acids

18.15

Weight (kDa)

9.12

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 61 - 136 2e-26 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 161
AclI AACGTT 1 cut(s) 248
AcoI YGGCCR 1 cut(s) 274
AcuI CTGAAG 2 cut(s) 317, 398
AdeI CACNNNGTG 1 cut(s) 161
AfaI GTAC 1 cut(s) 200
AfiI CCNNNNNNNGG 4 cut(s) 158, 161, 189, 272
AgsI TTSAA 5 cut(s) 216, 245, 253, 293, 458
AoxI GGCC 2 cut(s) 149, 274
AsuHPI GGTGA 1 cut(s) 41
BalI TGGCCA 1 cut(s) 276
BccI CCATC 1 cut(s) 115
BceAI ACGGC 1 cut(s) 452
BciVI GTATCC 1 cut(s) 118
BclI TGATCA 1 cut(s) 142
BfuI GTATCC 1 cut(s) 118
BmsI GCATC 6 cut(s) 195, 246, 268, 350, 372, 448
BpuEI CTTGAG 1 cut(s) 95
Bsc4I CCNNNNNNNGG 4 cut(s) 158, 161, 189, 272
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 349, 414
BseGI GGATG 1 cut(s) 201
BseLI CCNNNNNNNGG 4 cut(s) 158, 161, 189, 272
BseNI ACTGG 2 cut(s) 349, 414
BshFI GGCC 2 cut(s) 151, 276
BsiSI CCGG 1 cut(s) 183
BslFI GGGAC 1 cut(s) 402
BslI CCNNNNNNNGG 4 cut(s) 158, 161, 189, 272
BsmFI GGGAC 1 cut(s) 402
BsnI GGCC 2 cut(s) 151, 276
Bsp143I GATC 1 cut(s) 142
BspANI GGCC 2 cut(s) 151, 276
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 2 cut(s) 349, 414
BssAI RCCGGY 1 cut(s) 182
BssMI GATC 1 cut(s) 142
Bst4CI ACNGT 1 cut(s) 140
Bst6I CTCTTC 1 cut(s) 293
BstEII GGTNACC 1 cut(s) 29
BstF5I GGATG 1 cut(s) 201
BstKTI GATC 1 cut(s) 145
BstMBI GATC 1 cut(s) 142
BstPI GGTNACC 1 cut(s) 29
BsuI GTATCC 1 cut(s) 118
BsuRI GGCC 2 cut(s) 151, 276
BtsCI GGATG 1 cut(s) 201
BtsIMutI CAGTG 1 cut(s) 145
Cfr10I RCCGGY 1 cut(s) 182
Csp6I GTAC 1 cut(s) 199
CviJI RGCY 5 cut(s) 104, 151, 170, 276, 348
CviKI_1 RGCY 5 cut(s) 104, 151, 170, 276, 348
CviQI GTAC 1 cut(s) 199
DpnI GATC 1 cut(s) 144
DpnII GATC 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 161
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 293
EarI CTCTTC 1 cut(s) 293
Eco147I AGGCCT 1 cut(s) 151
Eco57I CTGAAG 2 cut(s) 317, 398
Eco91I GGTNACC 1 cut(s) 29
EcoO65I GGTNACC 1 cut(s) 29
EcoT22I ATGCAT 1 cut(s) 261
FaiI YATR 2 cut(s) 12, 333
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 402
FbaI TGATCA 1 cut(s) 142
FokI GGATG 1 cut(s) 208
HaeIII GGCC 2 cut(s) 151, 276
HapII CCGG 1 cut(s) 183
HincII GTYRAC 2 cut(s) 84, 232
HindII GTYRAC 2 cut(s) 84, 232
HinfI GANTC 2 cut(s) 76, 212
HpaII CCGG 1 cut(s) 183
HphI GGTGA 1 cut(s) 41
Hpy166II GTNNAC 3 cut(s) 52, 84, 232
Hpy188I TCNGA 3 cut(s) 147, 355, 378
Hpy188III TCNNGA 2 cut(s) 112, 216
Hpy8I GTNNAC 3 cut(s) 52, 84, 232
HpyAV CCTTC 1 cut(s) 277
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 248
HpyCH4V TGCA 3 cut(s) 259, 341, 410
HpySE526I ACGT 1 cut(s) 248
Ksp22I TGATCA 1 cut(s) 142
Kzo9I GATC 1 cut(s) 142
LpnPI CCDG 8 cut(s) 143, 186, 196, 222, 258, 362, 427, 443
LweI GCATC 6 cut(s) 195, 246, 268, 350, 372, 448
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 2 cut(s) 29, 285
MalI GATC 1 cut(s) 144
MboI GATC 1 cut(s) 142
MboII GAAGA 5 cut(s) 56, 59, 265, 310, 391
MfeI CAATTG 1 cut(s) 187
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 3 cut(s) 7, 187, 303
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 1 cut(s) 85
MmeI TCCRAC 2 cut(s) 333, 450
MnlI CCTC 9 cut(s) 97, 115, 141, 162, 186, 274, 313, 388, 433
Mox20I TGGCCA 1 cut(s) 276
Mph1103I ATGCAT 1 cut(s) 261
MscI TGGCCA 1 cut(s) 276
MseI TTAA 2 cut(s) 311, 478
Msp20I TGGCCA 1 cut(s) 276
MspI CCGG 1 cut(s) 183
MunI CAATTG 1 cut(s) 187
NdeII GATC 1 cut(s) 142
NmuCI GTSAC 1 cut(s) 29
NsiI ATGCAT 1 cut(s) 261
PceI AGGCCT 1 cut(s) 151
PfeI GAWTC 1 cut(s) 212
PflMI CCANNNNNTGG 1 cut(s) 161
PleI GAGTC 1 cut(s) 84
PpsI GAGTC 1 cut(s) 84
Psp1406I AACGTT 1 cut(s) 248
PspEI GGTNACC 1 cut(s) 29
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
SaqAI TTAA 2 cut(s) 311, 478
Sau3AI GATC 1 cut(s) 142
SchI GAGTC 1 cut(s) 85
SetI ASST 6 cut(s) 31, 89, 251, 317, 330, 432
SfaNI GCATC 6 cut(s) 195, 246, 268, 350, 372, 448
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
Sse9I AATT 3 cut(s) 7, 187, 303
SseBI AGGCCT 1 cut(s) 151
StuI AGGCCT 1 cut(s) 151
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 1 cut(s) 251
TasI AATT 3 cut(s) 7, 187, 303
TatI WGTACW 1 cut(s) 198
TfiI GAWTC 1 cut(s) 212
Tru1I TTAA 2 cut(s) 311, 478
Tru9I TTAA 2 cut(s) 311, 478
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 1 cut(s) 29
Tsp45I GTSAC 1 cut(s) 29
TspDTI ATGAA 1 cut(s) 59
TspRI CASTG 1 cut(s) 145
Van91I CCANNNNNTGG 1 cut(s) 161
Zsp2I ATGCAT 1 cut(s) 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.