Rh5BG521100

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000315
Physical Location & Seq
Forward (+)
82358495 .. 82360023
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG521100.1

Sequence Viewer

Length: 924 bp
ATGCAACCTCAAGTCTCCAATCAAGGACAACTCTTATCAGAGACCATTCAAAGTAACATTCCACCTGCTGGAGTTCAAAATTCTACTGGCTTATCATCTGCACTACCTCCTACCTCCGGGCCACAACAGCAAATATTGCCCCCACAACATCAACCGCAGCAATTAATTGCATCTTTGGATTCCACAGCCCAGACTGGGAATACAAATGGAGCTGATTGGAAAGAGGAGGTCTATCAAAAGTATCAAAAGAAGATGCAGAAGCTGCATATATTTCAACATGATGTACAACGACAGAAAGAGCAGCAGCAGCAGCTGCCTGGACAATTGCAGCCCGAGCTACACCAAATCAGTGTTGTTAACGACGTCAACACGAATGCCCTGTCATCATCACAGAGTGGGATTAACATGTTGCAGGCAAACGTTATTCCACCCAGTATTCTTCAGAATCAACTCCAAATCCAGCTACTACATCAGCGACAGATGCAGCTGCAGTTGATGGAGAAGCAGCAGAGAATTCAGCAACAGCAGCAAGCAAAGCAGCAGGTGCCTGGACTGTCACAGGCACACCAACTACCACAGCTTCACCAAATCAGTGTTGTTAAAGACGTTAATGTGGATGCCTCGTCATCACAAACTGGGAGTAGTATGCTGCAGGCAAACATTGATCCCCCTCAGCCAAAATCTATTATTGTTGATAACAAGCATCTGAAGCAAGATCAGGAGCAGAAAATGTTTCAGAATCAACTGAAGCAACCATATCAGCAGCAATGGATGCAGAAGCAGCAGATGCTTCAAGAAATGCAACAGCTGCAGATACAAAAACTACAACAGCAAGCAAAGCAGCAGCTGCATCATATTTATGTTATATATCTCATCTTTCTCACCATTCTGTTGGGTGCTGCCTTTTTCGGGACTGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

307

Amino Acids

34.91

Weight (kDa)

8.98

Isoelectric Point (pI)

66.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 73, 532
AatII GACGTC 1 cut(s) 366
Acc36I ACCTGC 2 cut(s) 73, 532
AccB1I GGYRCC 1 cut(s) 544
AccB7I CCANNNNNTGG 1 cut(s) 68
AciI CCGC 1 cut(s) 155
AclI AACGTT 1 cut(s) 420
AclWI GGATC 1 cut(s) 659
AcsI RAATTY 2 cut(s) 79, 513
AcuI CTGAAG 3 cut(s) 425, 728, 767
AcyI GRCGYC 1 cut(s) 363
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 4 cut(s) 68, 116, 195, 909
AflIII ACRYGT 1 cut(s) 405
AgsI TTSAA 4 cut(s) 50, 77, 275, 794
AjnI CCWGG 2 cut(s) 316, 547
AluBI AGCT 9 cut(s) 212, 262, 313, 337, 463, 487, 580, 808, 847
AluI AGCT 9 cut(s) 212, 262, 313, 337, 463, 487, 580, 808, 847
Alw26I GTCTC 2 cut(s) 19, 35
AlwI GGATC 1 cut(s) 659
AlwNI CAGNNNCTG 3 cut(s) 262, 313, 847
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 1 cut(s) 119
ApoI RAATTY 2 cut(s) 79, 513
ArsI GACNNNNNNTTYG 2 cut(s) 365, 397
AseI ATTAAT 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 119
AsuC2I CCSGG 1 cut(s) 118
AsuHPI GGTGA 2 cut(s) 575, 874
AvaI CYCGRG 1 cut(s) 332
BanI GGYRCC 1 cut(s) 544
BbvCI CCTCAGC 1 cut(s) 672
BccI CCATC 1 cut(s) 490
BciT130I CCWGG 2 cut(s) 318, 549
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 2 cut(s) 19, 35
BfmI CTRYAG 3 cut(s) 488, 650, 809
BfuAI ACCTGC 2 cut(s) 73, 532
Bme1390I CCNGG 3 cut(s) 118, 318, 549
BmeT110I CYCGRG 1 cut(s) 332
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 3 cut(s) 118, 318, 549
BmrI ACTGGG 3 cut(s) 204, 426, 645
BmsI GCATC 8 cut(s) 179, 243, 471, 607, 712, 762, 777, 859
BmuI ACTGGG 3 cut(s) 204, 426, 645
BpmI CTGGAG 1 cut(s) 90
Bpu10I CCTNAGC 1 cut(s) 672
BpuMI CCSGG 1 cut(s) 118
BsaHI GRCGYC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 35
Bsc4I CCNNNNNNNGG 4 cut(s) 68, 116, 195, 909
Bse1I ACTGG 5 cut(s) 91, 199, 432, 640, 919
Bse3DI GCAATG 1 cut(s) 773
BseBI CCWGG 2 cut(s) 318, 549
BseGI GGATG 2 cut(s) 622, 777
BseLI CCNNNNNNNGG 4 cut(s) 68, 116, 195, 909
BseMI GCAATG 1 cut(s) 773
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 5 cut(s) 91, 199, 432, 640, 919
BseRI GAGGAG 1 cut(s) 239
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 1 cut(s) 121
BshNI GGYRCC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 4 cut(s) 68, 116, 195, 909
BsmAI GTCTC 2 cut(s) 19, 35
BsmI GAATGC 1 cut(s) 379
BsnI GGCC 1 cut(s) 121
Bso31I GGTCTC 1 cut(s) 35
BsoBI CYCGRG 1 cut(s) 332
Bsp1407I TGTACA 1 cut(s) 283
Bsp143I GATC 2 cut(s) 664, 715
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 121
BspCNI CTCAG 1 cut(s) 685
BspLI GGNNCC 1 cut(s) 546
BspMAI CTGCAG 3 cut(s) 492, 654, 813
BspMI ACCTGC 2 cut(s) 73, 532
BspPI GGATC 1 cut(s) 659
BspT107I GGYRCC 1 cut(s) 544
BspTNI GGTCTC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 773
BsrGI TGTACA 1 cut(s) 283
BsrI ACTGG 5 cut(s) 91, 199, 432, 640, 919
BssMI GATC 2 cut(s) 664, 715
BssNI GRCGYC 1 cut(s) 363
Bst2UI CCWGG 2 cut(s) 318, 549
Bst4CI ACNGT 1 cut(s) 555
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 8 cut(s) 136, 262, 313, 544, 772, 787, 808, 847
BstAUI TGTACA 1 cut(s) 283
BstC8I GCNNGC 4 cut(s) 414, 531, 654, 834
BstDEI CTNAG 1 cut(s) 672
BstF5I GGATG 2 cut(s) 622, 777
BstKTI GATC 2 cut(s) 667, 718
BstMAI GTCTC 2 cut(s) 19, 35
BstMBI GATC 2 cut(s) 664, 715
BstNI CCWGG 2 cut(s) 318, 549
BstNSI RCATGY 1 cut(s) 409
BstSCI CCNGG 3 cut(s) 116, 316, 547
BstSFI CTRYAG 3 cut(s) 488, 650, 809
BstXI CCANNNNNNTGG 1 cut(s) 892
BsuRI GGCC 1 cut(s) 121
BtsCI GGATG 2 cut(s) 622, 777
BtsIMutI CAGTG 2 cut(s) 355, 598
BveI ACCTGC 2 cut(s) 73, 532
Cac8I GCNNGC 4 cut(s) 414, 531, 654, 834
CaiI CAGNNNCTG 3 cut(s) 262, 313, 847
Cfr13I GGNCC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 284
CviAII CATG 2 cut(s) 278, 406
CviQI GTAC 1 cut(s) 284
DdeI CTNAG 1 cut(s) 672
DpnI GATC 2 cut(s) 666, 717
DpnII GATC 2 cut(s) 664, 715
DraIII CACNNNGTG 1 cut(s) 395
Eco31I GGTCTC 1 cut(s) 35
Eco57I CTGAAG 3 cut(s) 425, 728, 767
Eco88I CYCGRG 1 cut(s) 332
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 316, 547
FaeI CATG 2 cut(s) 281, 409
FatI CATG 2 cut(s) 277, 405
FokI GGATG 2 cut(s) 629, 784
GsuI CTGGAG 1 cut(s) 90
HaeIII GGCC 1 cut(s) 121
HapII CCGG 1 cut(s) 117
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 2 cut(s) 281, 409
HincII GTYRAC 2 cut(s) 358, 367
HindII GTYRAC 2 cut(s) 358, 367
HinfI GANTC 3 cut(s) 179, 445, 739
HpaI GTTAAC 1 cut(s) 358
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 575, 874
Hpy166II GTNNAC 2 cut(s) 358, 367
Hpy188I TCNGA 4 cut(s) 40, 444, 708, 738
Hpy188III TCNNGA 3 cut(s) 719, 794, 910
Hpy8I GTNNAC 2 cut(s) 358, 367
Hpy99I CGWCG 1 cut(s) 365
HpyCH4III ACNGT 1 cut(s) 555
HpyCH4IV ACGT 3 cut(s) 363, 420, 606
HpyF3I CTNAG 1 cut(s) 672
HpySE526I ACGT 3 cut(s) 363, 420, 606
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 2 cut(s) 281, 409
KspAI GTTAAC 1 cut(s) 358
Kzo9I GATC 2 cut(s) 664, 715
LmnI GCTCC 2 cut(s) 209, 721
LweI GCATC 8 cut(s) 179, 243, 471, 607, 712, 762, 777, 859
MaeII ACGT 3 cut(s) 363, 420, 606
MaeIII GTNAC 2 cut(s) 53, 555
MalI GATC 2 cut(s) 666, 717
MboI GATC 2 cut(s) 664, 715
MboII GAAGA 2 cut(s) 262, 431
MfeI CAATTG 1 cut(s) 323
MluCI AATT 5 cut(s) 79, 161, 165, 323, 513
MnlI CCTC 7 cut(s) 18, 117, 124, 217, 220, 631, 681
MseI TTAA 5 cut(s) 164, 357, 402, 600, 609
MslI CAYNNNNRTG 1 cut(s) 858
MspA1I CMGCKG 4 cut(s) 313, 487, 808, 847
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 3 cut(s) 118, 318, 549
MunI CAATTG 1 cut(s) 323
Mva1269I GAATGC 1 cut(s) 379
MvaI CCWGG 2 cut(s) 318, 549
NciI CCSGG 1 cut(s) 118
NdeII GATC 2 cut(s) 664, 715
NlaIII CATG 2 cut(s) 281, 409
NlaIV GGNNCC 1 cut(s) 546
NmuCI GTSAC 1 cut(s) 555
NspI RCATGY 1 cut(s) 409
PaqCI CACCTGC 2 cut(s) 73, 532
PciI ACATGT 1 cut(s) 405
PctI GAATGC 1 cut(s) 379
PfeI GAWTC 3 cut(s) 179, 445, 739
PflMI CCANNNNNTGG 1 cut(s) 68
PscI ACATGT 1 cut(s) 405
PshBI ATTAAT 1 cut(s) 164
Psp1406I AACGTT 1 cut(s) 420
Psp6I CCWGG 2 cut(s) 316, 547
PspGI CCWGG 2 cut(s) 316, 547
PspN4I GGNNCC 1 cut(s) 546
PspPI GGNCC 1 cut(s) 119
PstI CTGCAG 3 cut(s) 492, 654, 813
PstNI CAGNNNCTG 3 cut(s) 262, 313, 847
PvuII CAGCTG 4 cut(s) 313, 487, 808, 847
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
RseI CAYNNNNRTG 1 cut(s) 858
SaqAI TTAA 5 cut(s) 164, 357, 402, 600, 609
Sau3AI GATC 2 cut(s) 664, 715
Sau96I GGNCC 1 cut(s) 119
ScrFI CCNGG 3 cut(s) 118, 318, 549
SfaNI GCATC 8 cut(s) 179, 243, 471, 607, 712, 762, 777, 859
SfcI CTRYAG 3 cut(s) 488, 650, 809
SmiMI CAYNNNNRTG 1 cut(s) 858
SmlI CTYRAG 1 cut(s) 9
SmoI CTYRAG 1 cut(s) 9
Sse9I AATT 5 cut(s) 79, 161, 165, 323, 513
SsiI CCGC 1 cut(s) 155
SspI AATATT 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 116, 316, 547
TaaI ACNGT 1 cut(s) 555
TaiI ACGT 3 cut(s) 366, 423, 609
TasI AATT 5 cut(s) 79, 161, 165, 323, 513
TatI WGTACW 1 cut(s) 283
TfiI GAWTC 3 cut(s) 179, 445, 739
Tru1I TTAA 5 cut(s) 164, 357, 402, 600, 609
Tru9I TTAA 5 cut(s) 164, 357, 402, 600, 609
TscAI CASTG 2 cut(s) 355, 598
TseFI GTSAC 1 cut(s) 555
Tsp45I GTSAC 1 cut(s) 555
TspRI CASTG 2 cut(s) 355, 598
Van91I CCANNNNNTGG 1 cut(s) 68
VspI ATTAAT 1 cut(s) 164
XapI RAATTY 2 cut(s) 79, 513
XceI RCATGY 1 cut(s) 409
ZraI GACGTC 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.