Rw5G046350

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000315
Physical Location & Seq
Forward (+)
80528434 .. 80535907
7474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G046350.1

Sequence Viewer

Length: 1722 bp
ATGTCTACATTGAAGGCGCATCGCCCCATTTCTGGTCAAGATGGATTAGATGAACTCGAGGAAAATGCTGTAAGTTTGGAGGAAAAGATTTATGCTGCTGCCACTAGCCAGTTGGACTATCTCTGGAAAGTTTCTCAGGAGATGCTCACAGTTGTGACCAAGCCACAGAATACAACATCCAATCCTCTAGAATCCAATGGCAAGGGACTTCTTGATTCAGTTGCTGATCAAGGACAATCAGTCTCAATGTCATTGCCAACCAATCAGTCCCAAACAAGGCAACATTTCGTACCACAGAACATACAGAATAACATTCTGCCTGCTGGAGTTCAAAGTTATACTGGGTTGTCATCTGGACTTTCTCCTACCATAAACTATCAACCAAGGGAACAATTTGTACCGCAAAATATTCAGAATAGCATTCTGACTGCTGGAGTTCAAAGTTCTGTTGGCTTATCATCTGCACTATCTCCTACCGGAAACTATCATCTGTATGGCCTCCAACCCCCTGCCCCTGGTCAAAATATTCCTGGCTTATCATATGCACTGCCTCCTCCACAGGGTCTGAATTTGTCTGCACTACTTGCCTCTATTCCGTCTGGCTTTTCTTCTGCTCCAGCATCTATTATGCGACCTTCCACACTGCAGTCATCTCTCCCCAGGCTTCAGCAGAATCAACAATCAACTATTCCCCACTCTTCACAATCCATGCTTCAGCAAACAGCAGTGCCACAACAGCAAATGTTGCCCCAACAGCAACAGTTAACAGATTCCACAACACATACAAATAGAGGTGATTGGCAAGAGGAGGTCTATCAAAAGATCATAGTCATGAAGGAGAAGTACTTGCCTGAACTAACTGAAATGTACCAGAAAATTGCTAATAAACTTCAGCAGCTTATACATATACAACAACGACAGTTGAGCATGCAACAACAGTTAATCCCACTACTGCAAGATATGCAGCAAAGGCACGAAGCATCAGGTCAGGTATCTGCAGCCATAATTCAACCACCAAATACAATGGAGCTGCAAAACCAGTTATATCAATCACATACAGCCCTTCCGGAGACATCATCAACATCTCTAGATTCGCCTGCCCTGACTGGACATACAGACAAGAGGCCTATCAAAAGGTTCATGAAGTACAACGATGGGAGTCCTCCTCAAGGTGGAGAGCCTGTAATGGATGCAGGAGATTGGAGGAGCCAATTGCAGGCGGATTCGCGGCAAAGAATTGTCAATAAGCTTATGGATCTATTGAAGAGGCATCTTCCTTTCTCCAGTCAAGAGGGGTTGAACGAACTCAAGAAAATTGCTGTTACGTTTGAGGAAAAGATTTATACTGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTGAAGATGCTCACTATGGAGACCAGGTCTCAGAAAACAATGGCCGATTCTTTACAATCCAACTCTGCTGTACGGTCTTCTGGTATGCAACCTCAAGTCACCAATCAAGGACAACTCTTATCAGAGAACATTCAAAGTAACATTCCGCCTACTGGAGTTCAAAATTCTGCTGGCTTATCATCTGCACTACCCCCTACCTCTGGGCCCGGCTTATCATCTGTACTATCTCCTACCCCGGCACTAATTACGTCTGCCTTACTATCTGCACTACCCCCTGCCCTGGTAGAAATATGCCTGCCAGCTTATCATCTGTGTTACCTTCTCCACAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

573

Amino Acids

62.68

Weight (kDa)

6.64

Isoelectric Point (pI)

72.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 1 - 61 3.2e-12 KIX domain
KIX_2 PF16987 399 - 477 3.2e-33 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 1228
AccIII TCCGGA 1 cut(s) 1066
AciI CCGC 4 cut(s) 401, 1220, 1228, 1532
AclWI GGATC 1 cut(s) 1263
AcoI YGGCCR 1 cut(s) 1428
AcsI RAATTY 3 cut(s) 568, 1380, 1549
AcuI CTGAAG 4 cut(s) 650, 698, 875, 1409
AfaI GTAC 7 cut(s) 291, 399, 845, 869, 1148, 1458, 1608
AgsI TTSAA 8 cut(s) 13, 332, 440, 1010, 1264, 1300, 1520, 1547
AhdI GACNNNNNGTC 1 cut(s) 239
AjnI CCWGG 5 cut(s) 514, 529, 659, 1409, 1665
AleI CACNNNNGTG 1 cut(s) 152
AluBI AGCT 4 cut(s) 898, 1030, 1249, 1688
AluI AGCT 4 cut(s) 898, 1030, 1249, 1688
Alw26I GTCTC 4 cut(s) 247, 1064, 1400, 1419
AlwI GGATC 1 cut(s) 1263
AlwNI CAGNNNCTG 2 cut(s) 224, 565
Ama87I CYCGRG 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 1066
AoxI GGCC 4 cut(s) 496, 1124, 1428, 1589
ApaI GGGCCC 1 cut(s) 1593
ApeKI GCWGC 7 cut(s) 95, 98, 895, 964, 998, 1030, 1349
ApoI RAATTY 3 cut(s) 568, 1380, 1549
AspLEI GCGC 1 cut(s) 19
AspS9I GGNCC 2 cut(s) 1589, 1590
AsuC2I CCSGG 2 cut(s) 1593, 1622
AsuHPI GGTGA 2 cut(s) 806, 1477
AvaI CYCGRG 1 cut(s) 56
BaeGI GKGCMC 1 cut(s) 1593
BanII GRGCYC 1 cut(s) 1593
BarI GAAGNNNNNNTAC 2 cut(s) 827, 859
BbsI GAAGAC 1 cut(s) 1455
BbvI GCAGC 7 cut(s) 82, 85, 907, 976, 1010, 1017, 1336
BccI CCATC 2 cut(s) 35, 1148
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BciT130I CCWGG 5 cut(s) 516, 531, 661, 1411, 1667
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 2 cut(s) 1593, 1622
BcoDI GTCTC 4 cut(s) 247, 1064, 1400, 1419
BfaI CTAG 3 cut(s) 105, 188, 1088
BfmI CTRYAG 2 cut(s) 644, 996
BisI GCNGC 8 cut(s) 96, 99, 896, 965, 999, 1031, 1229, 1350
BlsI GCNGC 8 cut(s) 97, 100, 897, 966, 1000, 1032, 1230, 1351
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 7 cut(s) 516, 531, 661, 1411, 1593, 1622, 1667
BmeRI GACNNNNNGTC 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 56
BmgT120I GGNCC 2 cut(s) 1589, 1590
BmiI GGNNCC 2 cut(s) 1208, 1591
BmrFI CCNGG 7 cut(s) 516, 531, 661, 1411, 1593, 1622, 1667
BmrI ACTGGG 1 cut(s) 351
BmsI GCATC 7 cut(s) 28, 132, 629, 989, 1180, 1279, 1383
BmuI ACTGGG 1 cut(s) 351
BpiI GAAGAC 1 cut(s) 1455
BplI GAGNNNNNCTC 2 cut(s) 1150, 1182
BpmI CTGGAG 5 cut(s) 345, 453, 600, 1267, 1560
BpuEI CTTGAG 3 cut(s) 1152, 1292, 1464
BpuMI CCSGG 2 cut(s) 1593, 1622
BsaI GGTCTC 2 cut(s) 1400, 1419
BsaJI CCNNGG 5 cut(s) 383, 514, 659, 1620, 1665
BsaWI WCCGGW 2 cut(s) 476, 1066
BsaXI ACNNNNNCTCC 4 cut(s) 1167, 1197, 1397, 1427
Bse1I ACTGG 6 cut(s) 109, 346, 1039, 1111, 1284, 1543
Bse3DI GCAATG 1 cut(s) 251
BseAI TCCGGA 1 cut(s) 1066
BseBI CCWGG 5 cut(s) 516, 531, 661, 1411, 1667
BseDI CCNNGG 5 cut(s) 383, 514, 659, 1620, 1665
BseGI GGATG 2 cut(s) 176, 1195
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 2 cut(s) 149, 1430
BseNI ACTGG 6 cut(s) 109, 346, 1039, 1111, 1284, 1543
BseRI GAGGAG 4 cut(s) 543, 821, 1155, 1219
BseSI GKGCMC 1 cut(s) 1593
BseXI GCAGC 7 cut(s) 82, 85, 907, 976, 1010, 1017, 1336
BsgI GTGCAG 4 cut(s) 447, 561, 1554, 1635
Bsh1236I CGCG 1 cut(s) 1228
BshFI GGCC 4 cut(s) 498, 1126, 1430, 1591
BsiHKCI CYCGRG 1 cut(s) 56
BsiSI CCGG 4 cut(s) 477, 1067, 1593, 1622
BslFI GGGAC 2 cut(s) 219, 253
BsmAI GTCTC 4 cut(s) 247, 1064, 1400, 1419
BsmFI GGGAC 2 cut(s) 219, 253
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 4 cut(s) 498, 1126, 1430, 1591
Bso31I GGTCTC 2 cut(s) 1400, 1419
BsoBI CYCGRG 1 cut(s) 56
Bsp120I GGGCCC 1 cut(s) 1589
Bsp1286I GDGCHC 1 cut(s) 1593
Bsp13I TCCGGA 1 cut(s) 1066
Bsp143I GATC 3 cut(s) 226, 822, 1255
BspACI CCGC 4 cut(s) 401, 1220, 1228, 1532
BspANI GGCC 4 cut(s) 498, 1126, 1430, 1591
BspCNI CTCAG 2 cut(s) 148, 1429
BspEI TCCGGA 1 cut(s) 1066
BspFNI CGCG 1 cut(s) 1228
BspHI TCATGA 2 cut(s) 831, 1140
BspLI GGNNCC 2 cut(s) 1208, 1591
BspMAI CTGCAG 2 cut(s) 648, 1000
BspPI GGATC 1 cut(s) 1263
BspTNI GGTCTC 2 cut(s) 1400, 1419
BsrDI GCAATG 1 cut(s) 251
BsrI ACTGG 6 cut(s) 109, 346, 1039, 1111, 1284, 1543
BssECI CCNNGG 5 cut(s) 383, 514, 659, 1620, 1665
BssMI GATC 3 cut(s) 226, 822, 1255
BssT1I CCWWGG 1 cut(s) 383
Bst2UI CCWGG 5 cut(s) 516, 531, 661, 1411, 1667
Bst4CI ACNGT 5 cut(s) 151, 762, 921, 939, 1461
Bst6I CTCTTC 2 cut(s) 703, 1259
BstAPI GCANNNNNTGC 3 cut(s) 584, 745, 961
BstC8I GCNNGC 7 cut(s) 321, 929, 1098, 1218, 1558, 1682, 1686
BstDEI CTNAG 2 cut(s) 135, 1416
BstENI CCTNNNNNAGG 1 cut(s) 1167
BstF5I GGATG 2 cut(s) 176, 1195
BstFNI CGCG 1 cut(s) 1228
BstHHI GCGC 1 cut(s) 19
BstKTI GATC 3 cut(s) 229, 825, 1258
BstMAI GTCTC 4 cut(s) 247, 1064, 1400, 1419
BstMBI GATC 3 cut(s) 226, 822, 1255
BstMWI GCNNNNNNNGC 6 cut(s) 584, 736, 745, 754, 961, 970
BstNI CCWGG 5 cut(s) 516, 531, 661, 1411, 1667
BstNSI RCATGY 1 cut(s) 931
BstSCI CCNGG 7 cut(s) 514, 529, 659, 1409, 1591, 1620, 1665
BstSFI CTRYAG 2 cut(s) 644, 996
BstSLI GKGCMC 1 cut(s) 1593
BstUI CGCG 1 cut(s) 1228
BstV1I GCAGC 7 cut(s) 82, 85, 907, 976, 1010, 1017, 1336
BstV2I GAAGAC 1 cut(s) 1455
BstX2I RGATCY 1 cut(s) 1255
BstYI RGATCY 1 cut(s) 1255
BsuRI GGCC 4 cut(s) 498, 1126, 1430, 1591
BtgZI GCGATG 1 cut(s) 5
BtsCI GGATG 2 cut(s) 176, 1195
BtsI GCAGTG 3 cut(s) 545, 641, 732
BtsIMutI CAGTG 3 cut(s) 545, 641, 732
Cac8I GCNNGC 7 cut(s) 321, 929, 1098, 1218, 1558, 1682, 1686
CaiI CAGNNNCTG 2 cut(s) 224, 565
CciI TCATGA 2 cut(s) 831, 1140
CfoI GCGC 1 cut(s) 19
Cfr13I GGNCC 2 cut(s) 1589, 1590
CsiI ACCWGGT 1 cut(s) 1409
Csp6I GTAC 7 cut(s) 290, 398, 844, 868, 1147, 1457, 1607
CviAII CATG 5 cut(s) 709, 832, 928, 1141, 1719
CviQI GTAC 7 cut(s) 290, 398, 844, 868, 1147, 1457, 1607
DdeI CTNAG 2 cut(s) 135, 1416
DpnI GATC 3 cut(s) 228, 824, 1257
DpnII GATC 3 cut(s) 226, 822, 1255
DriI GACNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 1428
Eam1104I CTCTTC 2 cut(s) 703, 1259
Eam1105I GACNNNNNGTC 1 cut(s) 239
EarI CTCTTC 2 cut(s) 703, 1259
EciI GGCGGA 2 cut(s) 1235, 1521
Eco130I CCWWGG 1 cut(s) 383
Eco147I AGGCCT 1 cut(s) 1126
Eco24I GRGCYC 1 cut(s) 1593
Eco31I GGTCTC 2 cut(s) 1400, 1419
Eco57I CTGAAG 4 cut(s) 650, 698, 875, 1409
Eco88I CYCGRG 1 cut(s) 56
EcoNI CCTNNNNNAGG 1 cut(s) 1167
EcoRII CCWGG 5 cut(s) 514, 529, 659, 1409, 1665
EcoT14I CCWWGG 1 cut(s) 383
EcoT38I GRGCYC 1 cut(s) 1593
ErhI CCWWGG 1 cut(s) 383
FaeI CATG 5 cut(s) 712, 835, 931, 1144, 1722
FaqI GGGAC 2 cut(s) 219, 253
FatI CATG 5 cut(s) 708, 831, 927, 1140, 1718
FauNDI CATATG 1 cut(s) 541
FbaI TGATCA 1 cut(s) 226
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 8 cut(s) 96, 99, 896, 965, 999, 1031, 1229, 1350
FokI GGATG 2 cut(s) 163, 1202
FriOI GRGCYC 1 cut(s) 1593
Fsp4HI GCNGC 8 cut(s) 96, 99, 896, 965, 999, 1031, 1229, 1350
FspBI CTAG 3 cut(s) 105, 188, 1088
GlaI GCGC 1 cut(s) 18
GluI GCNGC 8 cut(s) 96, 99, 896, 965, 999, 1031, 1229, 1350
GsuI CTGGAG 5 cut(s) 345, 453, 600, 1267, 1560
HaeIII GGCC 4 cut(s) 498, 1126, 1430, 1591
HapII CCGG 4 cut(s) 477, 1067, 1593, 1622
HhaI GCGC 1 cut(s) 19
Hin1II CATG 5 cut(s) 712, 835, 931, 1144, 1722
Hin6I GCGC 1 cut(s) 17
HinP1I GCGC 1 cut(s) 17
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 1247
HinfI GANTC 8 cut(s) 191, 215, 673, 770, 1091, 1159, 1223, 1433
HpaI GTTAAC 1 cut(s) 765
HpaII CCGG 4 cut(s) 477, 1067, 1593, 1622
HphI GGTGA 2 cut(s) 806, 1477
Hpy166II GTNNAC 2 cut(s) 6, 765
Hpy188I TCNGA 7 cut(s) 414, 426, 567, 1366, 1389, 1419, 1510
Hpy8I GTNNAC 2 cut(s) 6, 765
HpyAV CCTTC 5 cut(s) 7, 645, 829, 1073, 1715
HpyCH4III ACNGT 5 cut(s) 151, 762, 921, 939, 1461
HpyCH4IV ACGT 2 cut(s) 1325, 1634
HpyF10VI GCNNNNNNNGC 6 cut(s) 584, 736, 745, 754, 961, 970
HpyF3I CTNAG 2 cut(s) 135, 1416
HpySE526I ACGT 2 cut(s) 1325, 1634
Hsp92II CATG 5 cut(s) 712, 835, 931, 1144, 1722
HspAI GCGC 1 cut(s) 17
Kpn2I TCCGGA 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 226
KspAI GTTAAC 1 cut(s) 765
Kzo9I GATC 3 cut(s) 226, 822, 1255
LmnI GCTCC 3 cut(s) 619, 1027, 1206
Lsp1109I GCAGC 7 cut(s) 82, 85, 907, 976, 1010, 1017, 1336
LweI GCATC 7 cut(s) 28, 132, 629, 989, 1180, 1279, 1383
MabI ACCWGGT 1 cut(s) 1409
MaeI CTAG 3 cut(s) 105, 188, 1088
MaeII ACGT 2 cut(s) 1325, 1634
MaeIII GTNAC 5 cut(s) 154, 1321, 1483, 1523, 1700
MalI GATC 3 cut(s) 228, 824, 1257
MboI GATC 3 cut(s) 226, 822, 1255
MboII GAAGA 6 cut(s) 600, 690, 1265, 1276, 1402, 1455
MfeI CAATTG 1 cut(s) 1211
MflI RGATCY 1 cut(s) 1255
MhlI GDGCHC 1 cut(s) 1593
MlyI GAGTC 1 cut(s) 1168
MmeI TCCRAC 3 cut(s) 93, 526, 1470
MroI TCCGGA 1 cut(s) 1066
MseI TTAA 2 cut(s) 764, 941
MslI CAYNNNNRTG 3 cut(s) 152, 492, 830
MspI CCGG 4 cut(s) 477, 1067, 1593, 1622
MspR9I CCNGG 7 cut(s) 516, 531, 661, 1411, 1593, 1622, 1667
MunI CAATTG 1 cut(s) 1211
Mva1269I GAATGC 1 cut(s) 420
MvaI CCWGG 5 cut(s) 516, 531, 661, 1411, 1667
MvnI CGCG 1 cut(s) 1228
MwoI GCNNNNNNNGC 6 cut(s) 584, 736, 745, 754, 961, 970
NciI CCSGG 2 cut(s) 1593, 1622
NdeI CATATG 1 cut(s) 541
NdeII GATC 3 cut(s) 226, 822, 1255
NlaIII CATG 5 cut(s) 712, 835, 931, 1144, 1722
NlaIV GGNNCC 2 cut(s) 1208, 1591
NmuCI GTSAC 2 cut(s) 154, 1483
NspI RCATGY 1 cut(s) 931
OliI CACNNNNGTG 1 cut(s) 152
PaeI GCATGC 1 cut(s) 931
PaeR7I CTCGAG 1 cut(s) 56
PagI TCATGA 2 cut(s) 831, 1140
PceI AGGCCT 1 cut(s) 1126
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 7 cut(s) 191, 215, 673, 770, 1091, 1223, 1433
PflFI GACNNNGTC 1 cut(s) 1411
PkrI GCNGC 8 cut(s) 97, 100, 897, 966, 1000, 1032, 1230, 1351
PleI GAGTC 1 cut(s) 1167
PpsI GAGTC 1 cut(s) 1167
Psp6I CCWGG 5 cut(s) 514, 529, 659, 1409, 1665
PspGI CCWGG 5 cut(s) 514, 529, 659, 1409, 1665
PspN4I GGNNCC 2 cut(s) 1208, 1591
PspOMI GGGCCC 1 cut(s) 1589
PspPI GGNCC 2 cut(s) 1589, 1590
PspXI VCTCGAGB 1 cut(s) 56
PsrI GAACNNNNNNTAC 2 cut(s) 381, 413
PstI CTGCAG 2 cut(s) 648, 1000
PstNI CAGNNNCTG 2 cut(s) 224, 565
PsuI RGATCY 1 cut(s) 1255
PsyI GACNNNGTC 1 cut(s) 1411
RsaI GTAC 7 cut(s) 291, 399, 845, 869, 1148, 1458, 1608
RsaNI GTAC 7 cut(s) 290, 398, 844, 868, 1147, 1457, 1607
RseI CAYNNNNRTG 3 cut(s) 152, 492, 830
SaqAI TTAA 2 cut(s) 764, 941
SatI GCNGC 8 cut(s) 96, 99, 896, 965, 999, 1031, 1229, 1350
Sau3AI GATC 3 cut(s) 226, 822, 1255
Sau96I GGNCC 2 cut(s) 1589, 1590
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 1 cut(s) 1168
ScrFI CCNGG 7 cut(s) 516, 531, 661, 1411, 1593, 1622, 1667
SduI GDGCHC 1 cut(s) 1593
SexAI ACCWGGT 1 cut(s) 1409
SfaNI GCATC 7 cut(s) 28, 132, 629, 989, 1180, 1279, 1383
SfcI CTRYAG 2 cut(s) 644, 996
Sfr274I CTCGAG 1 cut(s) 56
SlaI CTCGAG 1 cut(s) 56
SmiMI CAYNNNNRTG 3 cut(s) 152, 492, 830
SmlI CTYRAG 4 cut(s) 56, 1167, 1307, 1479
SmoI CTYRAG 4 cut(s) 56, 1167, 1307, 1479
SphI GCATGC 1 cut(s) 931
SseBI AGGCCT 1 cut(s) 1126
SsiI CCGC 4 cut(s) 401, 1220, 1228, 1532
SspI AATATT 2 cut(s) 409, 526
SspMI CTAG 3 cut(s) 105, 188, 1088
StuI AGGCCT 1 cut(s) 1126
StyD4I CCNGG 7 cut(s) 514, 529, 659, 1409, 1591, 1620, 1665
StyI CCWWGG 1 cut(s) 383
TaaI ACNGT 5 cut(s) 151, 762, 921, 939, 1461
TaiI ACGT 2 cut(s) 1328, 1637
TaqI TCGA 1 cut(s) 57
TatI WGTACW 3 cut(s) 843, 1146, 1606
TauI GCSGC 1 cut(s) 1231
TfiI GAWTC 7 cut(s) 191, 215, 673, 770, 1091, 1223, 1433
Tru1I TTAA 2 cut(s) 764, 941
Tru9I TTAA 2 cut(s) 764, 941
TscAI CASTG 3 cut(s) 552, 648, 732
TseFI GTSAC 2 cut(s) 154, 1483
TseI GCWGC 7 cut(s) 95, 98, 895, 964, 998, 1030, 1349
Tsp45I GTSAC 2 cut(s) 154, 1483
TspDTI ATGAA 4 cut(s) 66, 848, 1129, 1157
TspGWI ACGGA 2 cut(s) 585, 1390
TspRI CASTG 3 cut(s) 552, 648, 732
Tth111I GACNNNGTC 1 cut(s) 1411
XagI CCTNNNNNAGG 1 cut(s) 1167
XapI RAATTY 3 cut(s) 568, 1380, 1549
XbaI TCTAGA 2 cut(s) 187, 1087
XceI RCATGY 1 cut(s) 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 3 cut(s) 105, 188, 1088
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.