MD03G1031600.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000315
Physical Location & Seq
Reverse (-)
2542687 .. 2545294
2608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1031600.v1.1.491

Sequence Viewer

Length: 291 bp
ATGGAGGCACGTGATTGGAGAAGCCAATTGCAGCAAGATTCACGACGCAGAATTGTTCACAAGATAATGGAAACGTTGAAGAGGCACCTTCCCTTCCAGGGCGAGGAGGGATTACGTGAACTCGAGAGGATTGCTGTAAGGTTTGAGGAAAAGATATATGTTGCTGCCTCAAGCCAGTCGGATTACCTACGGAAAATTTCTCTCAAGATGCTCACCATGGAGACCAAGAATCAGACCGCAGTGTCCGCGACACCCCCGTCCAACCTGAAGCGCCATCGCGTCCTTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

97

Amino Acids

11.36

Weight (kDa)

10.26

Isoelectric Point (pI)

78.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 4 - 82 5.2e-33 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 241, 256
AccB1I GGYRCC 1 cut(s) 84
AccII CGCG 2 cut(s) 248, 279
AciI CCGC 2 cut(s) 237, 246
AclI AACGTT 1 cut(s) 74
AcsI RAATTY 1 cut(s) 195
AcuI CTGAAG 1 cut(s) 287
AcvI CACGTG 1 cut(s) 11
AfiI CCNNNNNNNGG 2 cut(s) 98, 103
AgsI TTSAA 1 cut(s) 79
AjnI CCWGG 1 cut(s) 96
Alw26I GTCTC 1 cut(s) 215
Ama87I CYCGRG 1 cut(s) 122
ApeKI GCWGC 2 cut(s) 31, 164
ApoI RAATTY 1 cut(s) 195
AspLEI GCGC 1 cut(s) 273
AsuHPI GGTGA 1 cut(s) 205
AvaI CYCGRG 1 cut(s) 122
BanI GGYRCC 1 cut(s) 84
BbrPI CACGTG 1 cut(s) 11
BbvI GCAGC 2 cut(s) 43, 151
BccI CCATC 1 cut(s) 282
BcgI CGANNNNNNTGC 2 cut(s) 113, 147
BciT130I CCWGG 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 215
BfoI RGCGCY 1 cut(s) 274
BisI GCNGC 2 cut(s) 32, 165
BlsI GCNGC 2 cut(s) 33, 166
Bme1390I CCNGG 1 cut(s) 98
BmeT110I CYCGRG 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 198
BpuEI CTTGAG 2 cut(s) 154, 188
BsaAI YACGTR 2 cut(s) 11, 116
BsaI GGTCTC 1 cut(s) 215
BsaJI CCNNGG 2 cut(s) 97, 216
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 103
Bse1I ACTGG 1 cut(s) 175
BseBI CCWGG 1 cut(s) 98
BseDI CCNNGG 2 cut(s) 97, 216
BseLI CCNNNNNNNGG 2 cut(s) 98, 103
BseNI ACTGG 1 cut(s) 175
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 2 cut(s) 43, 151
Bsh1236I CGCG 2 cut(s) 248, 279
BshNI GGYRCC 1 cut(s) 84
BsiHKCI CYCGRG 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 98, 103
BsmAI GTCTC 1 cut(s) 215
Bso31I GGTCTC 1 cut(s) 215
BsoBI CYCGRG 1 cut(s) 122
Bsp19I CCATGG 1 cut(s) 216
BspACI CCGC 2 cut(s) 237, 246
BspFNI CGCG 2 cut(s) 248, 279
BspLI GGNNCC 1 cut(s) 86
BspT107I GGYRCC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 215
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 97, 216
BssT1I CCWWGG 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 98
Bst6I CTCTTC 1 cut(s) 74
BstBAI YACGTR 2 cut(s) 11, 116
BstDSI CCRYGG 1 cut(s) 216
BstFNI CGCG 2 cut(s) 248, 279
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 1 cut(s) 273
BstMAI GTCTC 1 cut(s) 215
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 98
BstSCI CCNGG 1 cut(s) 96
BstUI CGCG 2 cut(s) 248, 279
BstV1I GCAGC 2 cut(s) 43, 151
BtgI CCRYGG 1 cut(s) 216
BtgZI GCGATG 1 cut(s) 260
BtsI GCAGTG 1 cut(s) 246
BtsIMutI CAGTG 1 cut(s) 246
CfoI GCGC 1 cut(s) 273
CseI GACGC 2 cut(s) 54, 268
CviAII CATG 1 cut(s) 217
CviJI RGCY 2 cut(s) 24, 174
CviKI_1 RGCY 2 cut(s) 24, 174
DrdI GACNNNNNNGTC 2 cut(s) 241, 256
DseDI GACNNNNNNGTC 2 cut(s) 241, 256
Eam1104I CTCTTC 1 cut(s) 74
EarI CTCTTC 1 cut(s) 74
Eco130I CCWWGG 1 cut(s) 216
Eco31I GGTCTC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 287
Eco72I CACGTG 1 cut(s) 11
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 96
EcoT14I CCWWGG 1 cut(s) 216
ErhI CCWWGG 1 cut(s) 216
FaeI CATG 1 cut(s) 220
FaiI YATR 3 cut(s) 157, 159, 218
FatI CATG 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 32, 165
Fsp4HI GCNGC 2 cut(s) 32, 165
GlaI GCGC 1 cut(s) 272
GluI GCNGC 2 cut(s) 32, 165
HaeII RGCGCY 1 cut(s) 274
HgaI GACGC 2 cut(s) 54, 268
HhaI GCGC 1 cut(s) 273
Hin1II CATG 1 cut(s) 220
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HinfI GANTC 2 cut(s) 38, 229
HphI GGTGA 1 cut(s) 205
Hpy166II GTNNAC 2 cut(s) 58, 119
Hpy188I TCNGA 2 cut(s) 181, 234
Hpy188III TCNNGA 3 cut(s) 42, 124, 205
Hpy8I GTNNAC 2 cut(s) 58, 119
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 2 cut(s) 98, 103
HpyCH4IV ACGT 3 cut(s) 10, 74, 115
HpyCH4V TGCA 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpySE526I ACGT 3 cut(s) 10, 74, 115
Hsp92II CATG 1 cut(s) 220
HspAI GCGC 1 cut(s) 271
LpnPI CCDG 4 cut(s) 83, 110, 188, 278
Lsp1109I GCAGC 2 cut(s) 43, 151
LweI GCATC 1 cut(s) 198
MaeII ACGT 3 cut(s) 10, 74, 115
MboII GAAGA 1 cut(s) 91
MfeI CAATTG 1 cut(s) 26
MluCI AATT 3 cut(s) 26, 51, 195
MmeI TCCRAC 2 cut(s) 159, 285
MnlI CCTC 6 cut(s) 75, 97, 100, 120, 139, 178
MspR9I CCNGG 1 cut(s) 98
MunI CAATTG 1 cut(s) 26
MvaI CCWGG 1 cut(s) 98
MvnI CGCG 2 cut(s) 248, 279
MwoI GCNNNNNNNGC 1 cut(s) 245
NcoI CCATGG 1 cut(s) 216
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 1 cut(s) 86
PaeR7I CTCGAG 1 cut(s) 122
PfeI GAWTC 2 cut(s) 38, 229
PkrI GCNGC 2 cut(s) 33, 166
PmaCI CACGTG 1 cut(s) 11
PmlI CACGTG 1 cut(s) 11
Ppu21I YACGTR 2 cut(s) 11, 116
Psp1406I AACGTT 1 cut(s) 74
Psp6I CCWGG 1 cut(s) 96
PspCI CACGTG 1 cut(s) 11
PspGI CCWGG 1 cut(s) 96
PspN4I GGNNCC 1 cut(s) 86
SatI GCNGC 2 cut(s) 32, 165
ScrFI CCNGG 1 cut(s) 98
SetI ASST 7 cut(s) 13, 77, 90, 118, 143, 189, 267
SfaNI GCATC 1 cut(s) 198
Sfr274I CTCGAG 1 cut(s) 122
SlaI CTCGAG 1 cut(s) 122
SmlI CTYRAG 3 cut(s) 122, 169, 203
SmoI CTYRAG 3 cut(s) 122, 169, 203
Sse9I AATT 3 cut(s) 26, 51, 195
SsiI CCGC 2 cut(s) 237, 246
StyD4I CCNGG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 216
TaiI ACGT 3 cut(s) 13, 77, 118
TaqI TCGA 1 cut(s) 123
TasI AATT 3 cut(s) 26, 51, 195
TfiI GAWTC 2 cut(s) 38, 229
TscAI CASTG 1 cut(s) 246
TseI GCWGC 2 cut(s) 31, 164
TspGWI ACGGA 1 cut(s) 205
TspRI CASTG 1 cut(s) 246
XapI RAATTY 1 cut(s) 195
XhoI CTCGAG 1 cut(s) 122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.