Rroxscaffold_1G00005820

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000315
Physical Location & Seq
Reverse (-)
7855091 .. 7858099
3009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005820.1

Sequence Viewer

Length: 1110 bp
ATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATACTGCTGCCACAGCCAGATTATCTTCAGAAAATTTCTCTGAAGATGCTCACTATGGAGACCAAGTCTCAGACCACAATGGCCAATTCTTTACAATCCAACTCTGCTGGGTCTTCTGGTATGCAACCTCAAGTCACTAGTCAAGGACAACTGTTATCAGAGAACATTCAAAGTAACATTCCACCTGCTGGAGTTCAAAATTCTGCTGGCTTATCATCTGCACTACCTCCTACCTCTGGGCCACAACAGCAAATATTTCCCCCTCAGCATCAACCGCAAAAATTAATAGCATCTTTGGATTCCACAGCCCAGACTGGGAATACAAATGGAGCTGATTGGAAAGAGGAGGTCTGTCAAAAGTATCAAAAGAAGATGCAGAAGCTGTATATATTTCAACATGATGTACAACGACAGAAAGAGCTGCAGCAGCAGCTGCCTGGACAATTGCAGCCTGAGCTACACCAAATCAGTGTTGTTAACAACGTCAACACAAATGCCTTGTCATCATCACAGAGTGGGATTAACATGTTGCAGGCAAACGTTATTCCACCCAGGATTCTTCAGAAACAAAAAGTATATCAGAATCAACTCCAAATCCAGCTACTACATCAGCGACTGATGCAGCTGCAGTTGATGGAGAAGCAGCAAAGAATTCAGCAACAGCAACAGCAGCAAGCAAAGCAGCAGGTGCCTGGACTGTCACAGGCATACCAACTACCACAGCTTCACCAAATCAGTGTTGTTAAAGATGTTAACGTGGATGCCTCGTCATCACAAACTGGGAGTAGTATGCTGCAGGCAAACATTGATCCCCCTCAGCCAAATTCTATTACTGTTGATAACAAGCATCTGAAGCAAGATCAGGAGCAGAAAATGTTTCAGAATCAAGTAAAGCAACCATATCAGCAGCAATGGATGCAGAAGCAGCAGATGCTTCAAGAAATGCAACAGCTGCAGATACAAAAACTACAACAGCAAGCAAAGCAGCAGCTGTGTCATATTTATGTTATATATCTCATCTTTCTCACCATTCTGTTGGGTGCTGCCTTTTTCGGGACTGACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

369

Amino Acids

41.97

Weight (kDa)

9.6

Isoelectric Point (pI)

66.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 235, 718
Acc36I ACCTGC 2 cut(s) 235, 718
AccB1I GGYRCC 1 cut(s) 730
AccB7I CCANNNNNTGG 1 cut(s) 230
AciI CCGC 1 cut(s) 317
AclI AACGTT 1 cut(s) 582
AclWI GGATC 1 cut(s) 845
AcoI YGGCCR 1 cut(s) 123
AcsI RAATTY 4 cut(s) 75, 241, 693, 865
AcuI CTGAAG 4 cut(s) 53, 104, 587, 914
AdeI CACNNNGTG 1 cut(s) 557
AfaI GTAC 1 cut(s) 447
AfiI CCNNNNNNNGG 4 cut(s) 230, 278, 357, 1095
AflIII ACRYGT 1 cut(s) 567
AgsI TTSAA 4 cut(s) 212, 239, 437, 980
AhlI ACTAGT 1 cut(s) 179
AjnI CCWGG 3 cut(s) 478, 593, 733
Alw26I GTCTC 2 cut(s) 95, 114
AlwI GGATC 1 cut(s) 845
AlwNI CAGNNNCTG 4 cut(s) 424, 475, 658, 1033
AoxI GGCC 2 cut(s) 123, 281
ApoI RAATTY 4 cut(s) 75, 241, 693, 865
ArsI GACNNNNNNTTYG 2 cut(s) 527, 559
AseI ATTAAT 1 cut(s) 326
AspS9I GGNCC 1 cut(s) 281
AsuHPI GGTGA 2 cut(s) 761, 1060
BalI TGGCCA 1 cut(s) 125
BanI GGYRCC 1 cut(s) 730
BbsI GAAGAC 1 cut(s) 147
BbvCI CCTCAGC 2 cut(s) 306, 858
BccI CCATC 1 cut(s) 670
BciT130I CCWGG 3 cut(s) 480, 595, 735
BcoDI GTCTC 2 cut(s) 95, 114
BcuI ACTAGT 1 cut(s) 179
BfaI CTAG 1 cut(s) 180
BfmI CTRYAG 4 cut(s) 464, 668, 836, 995
BfuAI ACCTGC 2 cut(s) 235, 718
Bme1390I CCNGG 3 cut(s) 480, 595, 735
BmgT120I GGNCC 1 cut(s) 281
BmiI GGNNCC 1 cut(s) 732
BmrFI CCNGG 3 cut(s) 480, 595, 735
BmrI ACTGGG 2 cut(s) 366, 831
BmsI GCATC 9 cut(s) 78, 319, 341, 405, 651, 793, 898, 948, 963
BmuI ACTGGG 2 cut(s) 366, 831
BpiI GAAGAC 1 cut(s) 147
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 3 cut(s) 306, 495, 858
BpuEI CTTGAG 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 1 cut(s) 593
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bsc4I CCNNNNNNNGG 4 cut(s) 230, 278, 357, 1095
Bse1I ACTGG 2 cut(s) 361, 826
Bse3DI GCAATG 1 cut(s) 959
BseBI CCWGG 3 cut(s) 480, 595, 735
BseDI CCNNGG 1 cut(s) 593
BseGI GGATG 2 cut(s) 808, 963
BseLI CCNNNNNNNGG 4 cut(s) 230, 278, 357, 1095
BseMI GCAATG 1 cut(s) 959
BseMII CTCAG 4 cut(s) 125, 320, 486, 872
BseNI ACTGG 2 cut(s) 361, 826
BseRI GAGGAG 1 cut(s) 401
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 246
BshFI GGCC 2 cut(s) 125, 283
BshNI GGYRCC 1 cut(s) 730
BslI CCNNNNNNNGG 4 cut(s) 230, 278, 357, 1095
BsmAI GTCTC 2 cut(s) 95, 114
BsnI GGCC 2 cut(s) 125, 283
Bso31I GGTCTC 1 cut(s) 95
Bsp1407I TGTACA 1 cut(s) 445
Bsp143I GATC 2 cut(s) 850, 901
BspACI CCGC 1 cut(s) 317
BspANI GGCC 2 cut(s) 125, 283
BspCNI CTCAG 4 cut(s) 124, 319, 487, 871
BspLI GGNNCC 1 cut(s) 732
BspMAI CTGCAG 4 cut(s) 468, 672, 840, 999
BspMI ACCTGC 2 cut(s) 235, 718
BspPI GGATC 1 cut(s) 845
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 1 cut(s) 95
BsrDI GCAATG 1 cut(s) 959
BsrGI TGTACA 1 cut(s) 445
BsrI ACTGG 2 cut(s) 361, 826
BssECI CCNNGG 1 cut(s) 593
BssMI GATC 2 cut(s) 850, 901
Bst2UI CCWGG 3 cut(s) 480, 595, 735
Bst4CI ACNGT 3 cut(s) 195, 741, 877
BstAPI GCANNNNNTGC 5 cut(s) 475, 730, 958, 973, 994
BstAUI TGTACA 1 cut(s) 445
BstC8I GCNNGC 5 cut(s) 250, 576, 717, 840, 1020
BstDEI CTNAG 4 cut(s) 111, 306, 495, 858
BstF5I GGATG 2 cut(s) 808, 963
BstKTI GATC 2 cut(s) 853, 904
BstMAI GTCTC 2 cut(s) 95, 114
BstMBI GATC 2 cut(s) 850, 901
BstNI CCWGG 3 cut(s) 480, 595, 735
BstNSI RCATGY 1 cut(s) 571
BstSCI CCNGG 3 cut(s) 478, 593, 733
BstSFI CTRYAG 4 cut(s) 464, 668, 836, 995
BstV2I GAAGAC 1 cut(s) 147
BstXI CCANNNNNNTGG 1 cut(s) 1078
BsuRI GGCC 2 cut(s) 125, 283
BtsCI GGATG 2 cut(s) 808, 963
BtsIMutI CAGTG 2 cut(s) 517, 784
BveI ACCTGC 2 cut(s) 235, 718
Cac8I GCNNGC 5 cut(s) 250, 576, 717, 840, 1020
CaiI CAGNNNCTG 4 cut(s) 424, 475, 658, 1033
Cfr13I GGNCC 1 cut(s) 281
Csp6I GTAC 1 cut(s) 446
CviAII CATG 2 cut(s) 440, 568
CviQI GTAC 1 cut(s) 446
DdeI CTNAG 4 cut(s) 111, 306, 495, 858
DpnI GATC 2 cut(s) 852, 903
DpnII GATC 2 cut(s) 850, 901
DraIII CACNNNGTG 1 cut(s) 557
EaeI YGGCCR 1 cut(s) 123
Eco31I GGTCTC 1 cut(s) 95
Eco57I CTGAAG 4 cut(s) 53, 104, 587, 914
EcoRI GAATTC 1 cut(s) 693
EcoRII CCWGG 3 cut(s) 478, 593, 733
FaeI CATG 2 cut(s) 443, 571
FatI CATG 2 cut(s) 439, 567
FokI GGATG 2 cut(s) 815, 970
FspBI CTAG 1 cut(s) 180
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 2 cut(s) 125, 283
Hin1II CATG 2 cut(s) 443, 571
HincII GTYRAC 3 cut(s) 520, 529, 796
HindII GTYRAC 3 cut(s) 520, 529, 796
HinfI GANTC 4 cut(s) 341, 598, 625, 925
HpaI GTTAAC 2 cut(s) 520, 796
HphI GGTGA 2 cut(s) 761, 1060
Hpy166II GTNNAC 3 cut(s) 520, 529, 796
Hpy188I TCNGA 8 cut(s) 72, 84, 114, 202, 606, 624, 894, 924
Hpy188III TCNNGA 4 cut(s) 9, 905, 980, 1096
Hpy8I GTNNAC 3 cut(s) 520, 529, 796
HpyCH4III ACNGT 3 cut(s) 195, 741, 877
HpyCH4IV ACGT 3 cut(s) 525, 582, 798
HpyF3I CTNAG 4 cut(s) 111, 306, 495, 858
HpySE526I ACGT 3 cut(s) 525, 582, 798
Hsp92II CATG 2 cut(s) 443, 571
KspAI GTTAAC 2 cut(s) 520, 796
Kzo9I GATC 2 cut(s) 850, 901
LmnI GCTCC 2 cut(s) 371, 907
LweI GCATC 9 cut(s) 78, 319, 341, 405, 651, 793, 898, 948, 963
MaeI CTAG 1 cut(s) 180
MaeII ACGT 3 cut(s) 525, 582, 798
MaeIII GTNAC 3 cut(s) 175, 215, 741
MalI GATC 2 cut(s) 852, 903
MboI GATC 2 cut(s) 850, 901
MboII GAAGA 5 cut(s) 59, 97, 147, 424, 593
MfeI CAATTG 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 125
MluCI AATT 8 cut(s) 14, 75, 127, 241, 323, 485, 693, 865
MluNI TGGCCA 1 cut(s) 125
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 9 cut(s) 24, 180, 279, 286, 315, 379, 382, 817, 867
Mox20I TGGCCA 1 cut(s) 125
MscI TGGCCA 1 cut(s) 125
MseI TTAA 5 cut(s) 326, 519, 564, 786, 795
MslI CAYNNNNRTG 1 cut(s) 1044
Msp20I TGGCCA 1 cut(s) 125
MspA1I CMGCKG 4 cut(s) 475, 667, 994, 1033
MspR9I CCNGG 3 cut(s) 480, 595, 735
MunI CAATTG 1 cut(s) 485
MvaI CCWGG 3 cut(s) 480, 595, 735
NdeII GATC 2 cut(s) 850, 901
NlaIII CATG 2 cut(s) 443, 571
NlaIV GGNNCC 1 cut(s) 732
NmuCI GTSAC 2 cut(s) 175, 741
NspI RCATGY 1 cut(s) 571
PaqCI CACCTGC 2 cut(s) 235, 718
PciI ACATGT 1 cut(s) 567
PfeI GAWTC 4 cut(s) 341, 598, 625, 925
PflFI GACNNNGTC 1 cut(s) 106
PflMI CCANNNNNTGG 1 cut(s) 230
PscI ACATGT 1 cut(s) 567
PshBI ATTAAT 1 cut(s) 326
Psp1406I AACGTT 1 cut(s) 582
Psp6I CCWGG 3 cut(s) 478, 593, 733
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 3 cut(s) 478, 593, 733
PspN4I GGNNCC 1 cut(s) 732
PspPI GGNCC 1 cut(s) 281
PstI CTGCAG 4 cut(s) 468, 672, 840, 999
PstNI CAGNNNCTG 4 cut(s) 424, 475, 658, 1033
PsyI GACNNNGTC 1 cut(s) 106
PvuII CAGCTG 4 cut(s) 475, 667, 994, 1033
RsaI GTAC 1 cut(s) 447
RsaNI GTAC 1 cut(s) 446
RseI CAYNNNNRTG 1 cut(s) 1044
SaqAI TTAA 5 cut(s) 326, 519, 564, 786, 795
Sau3AI GATC 2 cut(s) 850, 901
Sau96I GGNCC 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 480, 595, 735
SfaNI GCATC 9 cut(s) 78, 319, 341, 405, 651, 793, 898, 948, 963
SfcI CTRYAG 4 cut(s) 464, 668, 836, 995
SmiMI CAYNNNNRTG 1 cut(s) 1044
SmlI CTYRAG 2 cut(s) 7, 171
SmoI CTYRAG 2 cut(s) 7, 171
SpeI ACTAGT 1 cut(s) 179
Sse9I AATT 8 cut(s) 14, 75, 127, 241, 323, 485, 693, 865
SsiI CCGC 1 cut(s) 317
SspI AATATT 1 cut(s) 297
SspMI CTAG 1 cut(s) 180
StyD4I CCNGG 3 cut(s) 478, 593, 733
TaaI ACNGT 3 cut(s) 195, 741, 877
TaiI ACGT 3 cut(s) 528, 585, 801
TasI AATT 8 cut(s) 14, 75, 127, 241, 323, 485, 693, 865
TatI WGTACW 1 cut(s) 445
TfiI GAWTC 4 cut(s) 341, 598, 625, 925
Tru1I TTAA 5 cut(s) 326, 519, 564, 786, 795
Tru9I TTAA 5 cut(s) 326, 519, 564, 786, 795
TscAI CASTG 2 cut(s) 517, 784
TseFI GTSAC 2 cut(s) 175, 741
Tsp45I GTSAC 2 cut(s) 175, 741
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 517, 784
Tth111I GACNNNGTC 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 230
VspI ATTAAT 1 cut(s) 326
XapI RAATTY 4 cut(s) 75, 241, 693, 865
XceI RCATGY 1 cut(s) 571
XspI CTAG 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.