Rh5CG544900

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000315
Physical Location & Seq
Forward (+)
76048964 .. 76053156
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG544900.1

Sequence Viewer

Length: 801 bp
ATGTATAGGTTTGAAAAGGAAACTGAAGGCATTGCCTTGGGTTTGGCCATTTGGGTAGAGAGTAGAGATCATATTCTGGCTTTATTGCGAATGCTAGTAGCTGCTGTTATAACATTGGAGGGTATGCAACTTCATCGGCAAAGGGAACTAGAGCAGTGGCATCTAAATCAACCCACCCACAAGAAGAAGAAGCAATTTCAGCGACTCTGCGACACTTGCGAGGTCGGTGGAAGCTTCCCAGATGAGGGATTGGGTGTCCAAGACAGGAATAGATCTGCCCAAGAGAAATTGGACCAAGCCCAGATTGAAGCTCTTCTCAAAGGTGGAGAGGAAGTCACTTCTGTCCTTGAAGAAATGATCACCCTCCCATTTTGTATCTTCAAATCCCTCCAAGAAAACGGTTTGATGGATAACAACAATGGGAGTCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCACTTGCAGGCAGATTCGCGGCAAAGAATTGTCAATAAGTTAACGGATATATTGAAGAGGCATATTCCTTACTCTGGTGAAGATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATGTTGCTGCCCCAAGTCAGTCAGATTATCTATCGAAAATTTCTCTAAAGATGCTCTCTTTGGAGACCAAGTCTCAGAACACAATGGCCAATTCTTTACAATCCAACTCTGCTGGGTCTTCTGGTATGCAACCTCAAGTCACCAGTCAAGGACAACTCTTATCAGAGAACATTCAAAGTAACATTCCACCTGCTGGAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

266

Amino Acids

29.7

Weight (kDa)

5.61

Isoelectric Point (pI)

59.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 154 - 231 6e-28 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 110
AarI CACCTGC 1 cut(s) 796
Acc36I ACCTGC 1 cut(s) 796
AccB7I CCANNNNNTGG 1 cut(s) 791
AccII CGCG 1 cut(s) 493
AciI CCGC 1 cut(s) 493
AcoI YGGCCR 2 cut(s) 45, 684
AcsI RAATTY 1 cut(s) 636
AcuI CTGAAG 1 cut(s) 45
AfiI CCNNNNNNNGG 7 cut(s) 244, 245, 434, 437, 481, 548, 791
AgsI TTSAA 6 cut(s) 14, 308, 350, 382, 529, 773
AluBI AGCT 3 cut(s) 101, 234, 311
AluI AGCT 3 cut(s) 101, 234, 311
Alw26I GTCTC 2 cut(s) 656, 675
AoxI GGCC 2 cut(s) 45, 684
ApeKI GCWGC 2 cut(s) 101, 605
ApoI RAATTY 1 cut(s) 636
Asp700I GAANNNNTTC 1 cut(s) 312
AspS9I GGNCC 1 cut(s) 292
AsuHPI GGTGA 3 cut(s) 352, 563, 730
AvaII GGWCC 1 cut(s) 292
BalI TGGCCA 2 cut(s) 47, 686
BbsI GAAGAC 1 cut(s) 708
BbvI GCAGC 2 cut(s) 88, 592
BccI CCATC 1 cut(s) 400
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BclI TGATCA 1 cut(s) 357
BcoDI GTCTC 2 cut(s) 656, 675
BfaI CTAG 2 cut(s) 95, 149
BfuAI ACCTGC 1 cut(s) 796
BglII AGATCT 1 cut(s) 272
BisI GCNGC 3 cut(s) 102, 494, 606
BlsI GCNGC 3 cut(s) 103, 495, 607
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 1 cut(s) 473
BmsI GCATC 3 cut(s) 169, 445, 639
BpiI GAAGAC 1 cut(s) 708
BplI GAGNNNNNCTC 2 cut(s) 415, 447
BpuEI CTTGAG 3 cut(s) 417, 548, 717
BsaI GGTCTC 1 cut(s) 656
BsaJI CCNNGG 1 cut(s) 36
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 653, 683
Bsc4I CCNNNNNNNGG 7 cut(s) 244, 245, 434, 437, 481, 548, 791
Bse1I ACTGG 1 cut(s) 741
Bse3DI GCAATG 2 cut(s) 30, 456
BseDI CCNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 460
BseLI CCNNNNNNNGG 7 cut(s) 244, 245, 434, 437, 481, 548, 791
BseMI GCAATG 2 cut(s) 30, 456
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 1 cut(s) 741
BseRI GAGGAG 2 cut(s) 420, 484
BseXI GCAGC 2 cut(s) 88, 592
BseYI CCCAGC 1 cut(s) 710
Bsh1236I CGCG 1 cut(s) 493
BshFI GGCC 2 cut(s) 47, 686
BslI CCNNNNNNNGG 7 cut(s) 244, 245, 434, 437, 481, 548, 791
BsmAI GTCTC 2 cut(s) 656, 675
BsmI GAATGC 1 cut(s) 96
BsnI GGCC 2 cut(s) 47, 686
Bso31I GGTCTC 1 cut(s) 656
Bsp143I GATC 3 cut(s) 67, 272, 357
BspACI CCGC 1 cut(s) 493
BspANI GGCC 2 cut(s) 47, 686
BspCNI CTCAG 1 cut(s) 685
BspFNI CGCG 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 473
BspMI ACCTGC 1 cut(s) 796
BspQI GCTCTTC 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 656
BsrDI GCAATG 2 cut(s) 30, 456
BsrI ACTGG 1 cut(s) 741
BssECI CCNNGG 1 cut(s) 36
BssMI GATC 3 cut(s) 67, 272, 357
BssT1I CCWWGG 1 cut(s) 36
Bst4CI ACNGT 1 cut(s) 401
Bst6I CTCTTC 2 cut(s) 318, 524
BstAPI GCANNNNNTGC 1 cut(s) 455
BstC8I GCNNGC 2 cut(s) 447, 483
BstDEI CTNAG 1 cut(s) 672
BstENI CCTNNNNNAGG 1 cut(s) 432
BstF5I GGATG 1 cut(s) 460
BstFNI CGCG 1 cut(s) 493
BstKTI GATC 3 cut(s) 70, 275, 360
BstMAI GTCTC 2 cut(s) 656, 675
BstMBI GATC 3 cut(s) 67, 272, 357
BstMWI GCNNNNNNNGC 3 cut(s) 199, 216, 455
BstUI CGCG 1 cut(s) 493
BstV1I GCAGC 2 cut(s) 88, 592
BstV2I GAAGAC 1 cut(s) 708
BstX2I RGATCY 1 cut(s) 272
BstYI RGATCY 1 cut(s) 272
BsuRI GGCC 2 cut(s) 47, 686
BtsCI GGATG 1 cut(s) 460
BtsI GCAGTG 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 161
BveI ACCTGC 1 cut(s) 796
Cac8I GCNNGC 2 cut(s) 447, 483
Cfr13I GGNCC 1 cut(s) 292
CviJI RGCY 9 cut(s) 47, 80, 101, 234, 299, 311, 445, 474, 686
CviKI_1 RGCY 9 cut(s) 47, 80, 101, 234, 299, 311, 445, 474, 686
DdeI CTNAG 1 cut(s) 672
DpnI GATC 3 cut(s) 69, 274, 359
DpnII GATC 3 cut(s) 67, 272, 357
EaeI YGGCCR 2 cut(s) 45, 684
Eam1104I CTCTTC 2 cut(s) 318, 524
EarI CTCTTC 2 cut(s) 318, 524
Eco130I CCWWGG 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 656
Eco47I GGWCC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 45
EcoNI CCTNNNNNAGG 1 cut(s) 432
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
FaiI YATR 8 cut(s) 6, 72, 110, 125, 524, 537, 600, 725
FbaI TGATCA 1 cut(s) 357
Fnu4HI GCNGC 3 cut(s) 102, 494, 606
FokI GGATG 1 cut(s) 467
Fsp4HI GCNGC 3 cut(s) 102, 494, 606
FspBI CTAG 2 cut(s) 95, 149
GluI GCNGC 3 cut(s) 102, 494, 606
GsaI CCCAGC 1 cut(s) 714
HaeIII GGCC 2 cut(s) 47, 686
HincII GTYRAC 1 cut(s) 516
HindII GTYRAC 1 cut(s) 516
HindIII AAGCTT 1 cut(s) 232
HinfI GANTC 3 cut(s) 204, 424, 488
HpaI GTTAAC 1 cut(s) 516
HphI GGTGA 3 cut(s) 352, 563, 730
Hpy166II GTNNAC 1 cut(s) 516
Hpy188I TCNGA 3 cut(s) 622, 675, 763
Hpy188III TCNNGA 1 cut(s) 565
Hpy8I GTNNAC 1 cut(s) 516
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4V TGCA 5 cut(s) 127, 449, 458, 481, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 199, 216, 455
HpyF3I CTNAG 1 cut(s) 672
Ksp22I TGATCA 1 cut(s) 357
KspAI GTTAAC 1 cut(s) 516
Kzo9I GATC 3 cut(s) 67, 272, 357
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 471
Lsp1109I GCAGC 2 cut(s) 88, 592
LweI GCATC 3 cut(s) 169, 445, 639
MaeI CTAG 2 cut(s) 95, 149
MaeIII GTNAC 3 cut(s) 334, 736, 776
MalI GATC 3 cut(s) 69, 274, 359
MboI GATC 3 cut(s) 67, 272, 357
MboII GAAGA 8 cut(s) 196, 199, 305, 362, 370, 541, 566, 708
MflI RGATCY 1 cut(s) 272
MlsI TGGCCA 2 cut(s) 47, 686
MluCI AATT 6 cut(s) 194, 287, 501, 570, 636, 688
MluNI TGGCCA 2 cut(s) 47, 686
MlyI GAGTC 2 cut(s) 198, 433
MmeI TCCRAC 1 cut(s) 726
Mox20I TGGCCA 2 cut(s) 47, 686
MroXI GAANNNNTTC 1 cut(s) 312
MscI TGGCCA 2 cut(s) 47, 686
MseI TTAA 2 cut(s) 515, 799
Msp20I TGGCCA 2 cut(s) 47, 686
Mva1269I GAATGC 1 cut(s) 96
MvnI CGCG 1 cut(s) 493
MwoI GCNNNNNNNGC 3 cut(s) 199, 216, 455
NdeII GATC 3 cut(s) 67, 272, 357
NlaIV GGNNCC 1 cut(s) 473
NmuCI GTSAC 2 cut(s) 334, 736
PaqCI CACCTGC 1 cut(s) 796
PciSI GCTCTTC 1 cut(s) 318
PctI GAATGC 1 cut(s) 96
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 1 cut(s) 488
PflFI GACNNNGTC 1 cut(s) 667
PflMI CCANNNNNTGG 1 cut(s) 791
PkrI GCNGC 3 cut(s) 103, 495, 607
PleI GAGTC 2 cut(s) 198, 432
PpsI GAGTC 2 cut(s) 198, 432
PsiI TTATAA 1 cut(s) 110
PspFI CCCAGC 1 cut(s) 710
PspN4I GGNNCC 1 cut(s) 473
PspPI GGNCC 1 cut(s) 292
PsuI RGATCY 1 cut(s) 272
PsyI GACNNNGTC 1 cut(s) 667
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 2 cut(s) 515, 799
SatI GCNGC 3 cut(s) 102, 494, 606
Sau3AI GATC 3 cut(s) 67, 272, 357
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 2 cut(s) 198, 433
SfaNI GCATC 3 cut(s) 169, 445, 639
SinI GGWCC 1 cut(s) 292
SmlI CTYRAG 3 cut(s) 432, 563, 732
SmoI CTYRAG 3 cut(s) 432, 563, 732
Sse9I AATT 6 cut(s) 194, 287, 501, 570, 636, 688
SsiI CCGC 1 cut(s) 493
SspMI CTAG 2 cut(s) 95, 149
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 401
TaqI TCGA 1 cut(s) 632
TasI AATT 6 cut(s) 194, 287, 501, 570, 636, 688
TauI GCSGC 1 cut(s) 496
TfiI GAWTC 1 cut(s) 488
Tru1I TTAA 2 cut(s) 515, 799
Tru9I TTAA 2 cut(s) 515, 799
TscAI CASTG 1 cut(s) 161
TseFI GTSAC 2 cut(s) 334, 736
TseI GCWGC 2 cut(s) 101, 605
Tsp45I GTSAC 2 cut(s) 334, 736
TspDTI ATGAA 2 cut(s) 122, 573
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 1 cut(s) 161
Tth111I GACNNNGTC 1 cut(s) 667
Van91I CCANNNNNTGG 1 cut(s) 791
VpaK11BI GGWCC 1 cut(s) 292
XagI CCTNNNNNAGG 1 cut(s) 432
XapI RAATTY 1 cut(s) 636
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 95, 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.