Rh5CG544400

KIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000315
Physical Location & Seq
Forward (+)
75966958 .. 75972036
5079 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG544400.1

Sequence Viewer

Length: 621 bp
ATGGATATCAATATCGATACTGAGATGCATCTGCAAGGTGGAGAACCCTTGATGGTCTCCGTCGATTGGCGTAGCTACTTGATGCCAGAATCACGAGAAAGATTACAGGAACTCCAGAAAATGGATTTAAGGTTTGAGGAAAAGGTTTATACTGCTGCCACAAGCCAGTTGGATTATCTGCGGAAAATTTCTGTGAAAATGCTAACAATGGATAGATCAAGTCTCAGAACACAATGGCCAACTCCTTGCAATTCAACTCTGCTGGAAATAGCAACACAACCCCGATCCATTGATCAGTGGCCTCCACAGGGTGGAGAAGCCTTGAATGGCGCCGGCGATTGGAGAATCTACTTGATGCCAGATTCAAGGCAAAGAATTGTCAGAAGATGTAAAACTGATGTGTTGAAGAGGCATCTTCCCTTCTCTGGCCAAGAGGGGTTACTTGAACGGCAGAGAATTGCTGCAAGGTTCGAGGATAAGTTGTATGCTACTGCATCAAGCCAGTCAGATTATCTACGAAAAATTTCTCTGAAGTTGCTTGCCATTGAGACCTCGGGAGTTGCAACAAGTCCCCCCTCCAACCTGAAACGGCATCGTGCCCTTGCTTTTGAAAGAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

206

Amino Acids

23.8

Weight (kDa)

9.12

Isoelectric Point (pI)

67.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 28 - 75 1.6e-12 KIX domain
KIX_2 PF16987 112 - 185 3.1e-22 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 329
AccB7I CCANNNNNTGG 2 cut(s) 121, 311
AciI CCGC 1 cut(s) 181
AclWI GGATC 1 cut(s) 279
AcoI YGGCCR 2 cut(s) 236, 427
AcsI RAATTY 2 cut(s) 186, 522
AcuI CTGAAG 1 cut(s) 551
AcyI GRCGYC 1 cut(s) 330
AdeI CACNNNGTG 1 cut(s) 311
AfiI CCNNNNNNNGG 6 cut(s) 66, 121, 308, 311, 339, 425
AgsI TTSAA 6 cut(s) 255, 325, 366, 406, 446, 611
AluBI AGCT 1 cut(s) 75
AluI AGCT 1 cut(s) 75
Alw26I GTCTC 4 cut(s) 61, 227, 542, 609
AlwI GGATC 1 cut(s) 279
Ama87I CYCGRG 1 cut(s) 553
AoxI GGCC 3 cut(s) 236, 299, 427
ApeKI GCWGC 2 cut(s) 155, 461
ApoI RAATTY 2 cut(s) 186, 522
Asp700I GAANNNNTTC 1 cut(s) 523
AspLEI GCGC 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 553
BaeGI GKGCMC 1 cut(s) 601
BalI TGGCCA 2 cut(s) 238, 429
BanI GGYRCC 1 cut(s) 329
BauI CACGAG 1 cut(s) 93
BbvI GCAGC 2 cut(s) 142, 448
BccI CCATC 1 cut(s) 46
BceAI ACGGC 2 cut(s) 464, 605
BclI TGATCA 1 cut(s) 292
BcoDI GTCTC 4 cut(s) 61, 227, 542, 609
BfoI RGCGCY 1 cut(s) 333
BisI GCNGC 2 cut(s) 156, 462
BlsI GCNGC 2 cut(s) 157, 463
BmeT110I CYCGRG 1 cut(s) 553
BmiI GGNNCC 1 cut(s) 331
BmsI GCATC 7 cut(s) 15, 37, 72, 345, 421, 503, 601
BpmI CTGGAG 1 cut(s) 98
Bsa29I ATCGAT 1 cut(s) 15
BsaHI GRCGYC 1 cut(s) 330
BsaI GGTCTC 2 cut(s) 61, 542
BsaJI CCNNGG 1 cut(s) 552
BsaXI ACNNNNNCTCC 2 cut(s) 96, 126
Bsc4I CCNNNNNNNGG 6 cut(s) 66, 121, 308, 311, 339, 425
Bse118I RCCGGY 1 cut(s) 332
Bse1I ACTGG 2 cut(s) 166, 502
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 1 cut(s) 552
BseLI CCNNNNNNNGG 6 cut(s) 66, 121, 308, 311, 339, 425
BseMII CTCAG 2 cut(s) 12, 238
BseNI ACTGG 2 cut(s) 166, 502
BseSI GKGCMC 1 cut(s) 601
BseXI GCAGC 2 cut(s) 142, 448
BshFI GGCC 3 cut(s) 238, 301, 429
BshNI GGYRCC 1 cut(s) 329
BshVI ATCGAT 1 cut(s) 15
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 1 cut(s) 333
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 6 cut(s) 66, 121, 308, 311, 339, 425
BsmAI GTCTC 4 cut(s) 61, 227, 542, 609
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 3 cut(s) 238, 301, 429
Bso31I GGTCTC 2 cut(s) 61, 542
BsoBI CYCGRG 1 cut(s) 553
Bsp1286I GDGCHC 1 cut(s) 601
Bsp143I GATC 3 cut(s) 215, 284, 292
BspACI CCGC 1 cut(s) 181
BspANI GGCC 3 cut(s) 238, 301, 429
BspCNI CTCAG 2 cut(s) 13, 237
BspDI ATCGAT 1 cut(s) 15
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 1 cut(s) 279
BspT107I GGYRCC 1 cut(s) 329
BspTNI GGTCTC 2 cut(s) 61, 542
BsrFI RCCGGY 1 cut(s) 332
BsrI ACTGG 2 cut(s) 166, 502
BssAI RCCGGY 1 cut(s) 332
BssECI CCNNGG 1 cut(s) 552
BssMI GATC 3 cut(s) 215, 284, 292
BssNI GRCGYC 1 cut(s) 330
BssSI CACGAG 1 cut(s) 93
Bst2BI CACGAG 1 cut(s) 93
Bst6I CTCTTC 1 cut(s) 401
BstACI GRCGYC 1 cut(s) 330
BstC8I GCNNGC 2 cut(s) 334, 540
BstDEI CTNAG 2 cut(s) 21, 224
BstH2I RGCGCY 1 cut(s) 333
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 218, 287, 295
BstMAI GTCTC 4 cut(s) 61, 227, 542, 609
BstMBI GATC 3 cut(s) 215, 284, 292
BstSLI GKGCMC 1 cut(s) 601
BstV1I GCAGC 2 cut(s) 142, 448
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 3 cut(s) 238, 301, 429
BsuTUI ATCGAT 1 cut(s) 15
BtsIMutI CAGTG 1 cut(s) 302
Cac8I GCNNGC 2 cut(s) 334, 540
CfoI GCGC 1 cut(s) 332
Cfr10I RCCGGY 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 15
CviJI RGCY 7 cut(s) 75, 165, 238, 301, 320, 429, 501
CviKI_1 RGCY 7 cut(s) 75, 165, 238, 301, 320, 429, 501
DdeI CTNAG 2 cut(s) 21, 224
DinI GGCGCC 1 cut(s) 331
DpnI GATC 3 cut(s) 217, 286, 294
DpnII GATC 3 cut(s) 215, 284, 292
DraIII CACNNNGTG 1 cut(s) 311
EaeI YGGCCR 2 cut(s) 236, 427
Eam1104I CTCTTC 1 cut(s) 401
EarI CTCTTC 1 cut(s) 401
Eco31I GGTCTC 2 cut(s) 61, 542
Eco32I GATATC 1 cut(s) 7
Eco57I CTGAAG 1 cut(s) 551
Eco88I CYCGRG 1 cut(s) 553
EcoRV GATATC 1 cut(s) 7
EcoT22I ATGCAT 1 cut(s) 30
EgeI GGCGCC 1 cut(s) 331
EheI GGCGCC 1 cut(s) 331
FaiI YATR 2 cut(s) 150, 486
FaqI GGGAC 1 cut(s) 555
FbaI TGATCA 1 cut(s) 292
Fnu4HI GCNGC 2 cut(s) 156, 462
Fsp4HI GCNGC 2 cut(s) 156, 462
GlaI GCGC 1 cut(s) 331
GluI GCNGC 2 cut(s) 156, 462
GsuI CTGGAG 1 cut(s) 98
HaeII RGCGCY 1 cut(s) 333
HaeIII GGCC 3 cut(s) 238, 301, 429
HapII CCGG 1 cut(s) 333
HhaI GCGC 1 cut(s) 332
Hin1I GRCGYC 1 cut(s) 330
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HinfI GANTC 3 cut(s) 89, 345, 362
HpaII CCGG 1 cut(s) 333
Hpy188I TCNGA 4 cut(s) 227, 383, 508, 531
Hpy188III TCNNGA 3 cut(s) 93, 115, 555
Hpy99I CGWCG 1 cut(s) 65
HpyAV CCTTC 1 cut(s) 430
HpyCH4V TGCA 6 cut(s) 28, 34, 249, 464, 494, 563
HpyF3I CTNAG 2 cut(s) 21, 224
Hsp92I GRCGYC 1 cut(s) 330
HspAI GCGC 1 cut(s) 330
KasI GGCGCC 1 cut(s) 329
KroI GCCGGC 1 cut(s) 332
KroNI GCCGGC 1 cut(s) 334
Ksp22I TGATCA 1 cut(s) 292
Kzo9I GATC 3 cut(s) 215, 284, 292
Lsp1109I GCAGC 2 cut(s) 142, 448
LweI GCATC 7 cut(s) 15, 37, 72, 345, 421, 503, 601
MaeIII GTNAC 1 cut(s) 438
MalI GATC 3 cut(s) 217, 286, 294
MboI GATC 3 cut(s) 215, 284, 292
MboII GAAGA 3 cut(s) 396, 407, 418
MhlI GDGCHC 1 cut(s) 601
MlsI TGGCCA 2 cut(s) 238, 429
MluCI AATT 5 cut(s) 186, 250, 375, 456, 522
MluNI TGGCCA 2 cut(s) 238, 429
Mly113I GGCGCC 1 cut(s) 330
MmeI TCCRAC 2 cut(s) 150, 603
MnlI CCTC 7 cut(s) 130, 312, 402, 427, 466, 562, 586
Mox20I TGGCCA 2 cut(s) 238, 429
Mph1103I ATGCAT 1 cut(s) 30
MreI CGCCGGCG 1 cut(s) 332
MroNI GCCGGC 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 523
MscI TGGCCA 2 cut(s) 238, 429
MseI TTAA 1 cut(s) 128
Msp20I TGGCCA 2 cut(s) 238, 429
MspI CCGG 1 cut(s) 333
NaeI GCCGGC 1 cut(s) 334
NarI GGCGCC 1 cut(s) 330
NdeII GATC 3 cut(s) 215, 284, 292
NgoMIV GCCGGC 1 cut(s) 332
NlaIV GGNNCC 1 cut(s) 331
NsiI ATGCAT 1 cut(s) 30
PdiI GCCGGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 523
PfeI GAWTC 3 cut(s) 89, 345, 362
PflMI CCANNNNNTGG 2 cut(s) 121, 311
PkrI GCNGC 2 cut(s) 157, 463
PluTI GGCGCC 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 331
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 156, 462
Sau3AI GATC 3 cut(s) 215, 284, 292
SduI GDGCHC 1 cut(s) 601
SetI ASST 7 cut(s) 40, 77, 134, 147, 470, 554, 585
SfaNI GCATC 7 cut(s) 15, 37, 72, 345, 421, 503, 601
SfoI GGCGCC 1 cut(s) 331
SgrAI CRCCGGYG 1 cut(s) 332
Sse9I AATT 5 cut(s) 186, 250, 375, 456, 522
SsiI CCGC 1 cut(s) 181
SspDI GGCGCC 1 cut(s) 329
TaqI TCGA 3 cut(s) 15, 63, 471
TasI AATT 5 cut(s) 186, 250, 375, 456, 522
TfiI GAWTC 3 cut(s) 89, 345, 362
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 1 cut(s) 302
TseI GCWGC 2 cut(s) 155, 461
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 1 cut(s) 302
Van91I CCANNNNNTGG 2 cut(s) 121, 311
XapI RAATTY 2 cut(s) 186, 522
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XmnI GAANNNNTTC 1 cut(s) 523
Zsp2I ATGCAT 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.