Rorug05G0442600

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000315
Physical Location & Seq
Forward (+)
61455949 .. 61456695
747 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0442600.1

Sequence Viewer

Length: 630 bp
ATGCTAGCAGCTGGTACTTGTTTCCCGCTTGCTCCTCTCTTCCTTAGTCACCTATACAGGAAGCTTGATCAGATTTCAAAGGATGATAAAGAAGGTGCGGGGCAGTATGGCATTGATTCTTTAGTATCTAGCTTCTTCCTTCAACTATGGTTCAGAAGAAAAGTCAAGAAGGGTGCATTTAGGCCTGCTATCCTGGATGACATCGGACAATTCAATTTCAGGCCGTATTTTACTGCTCACGGCTTCCAGACATTTCCTTTCTTTTATCCAGAGGGTAACTCATCACTGTTCTTAGGATCCGTCGAGCTGCTTCTAGCCACTAATGCGCAGACCATAATAGCATATGGTGATTTCTTTGACCTTGCGATGGAGATATATTCCCCTCATAGAGTTAAGAGGCAATTTGGAATCGATCAAGATGTGCCATCGCATCTATCTTACGATGGACATCTCCGGAATTGCATCATGGATTTCACTCGGACATATGGTATTTCTTGCAGTGGTATACTTTTTGATAGTCTCGGGAAAAAGGACAGTGTCAGCTCCCCTACATTAACATTCTTGAGATTTTGGGAGCTACAGCGGTGCGAGTTCAGGAGGTTTATTAAGACCAGTGGTACTAGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

209

Amino Acids

23.95

Weight (kDa)

8.88

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMD PF10536 122 - 145 7.6e-06 Plant mobile domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 327
AccI GTMKAC 1 cut(s) 505
AccIII TCCGGA 1 cut(s) 453
AciI CCGC 3 cut(s) 26, 98, 583
AclWI GGATC 2 cut(s) 291, 304
AfaI GTAC 2 cut(s) 16, 619
AfiI CCNNNNNNNGG 1 cut(s) 367
AgsI TTSAA 3 cut(s) 78, 143, 214
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 6 cut(s) 11, 64, 132, 307, 543, 577
AluI AGCT 6 cut(s) 11, 64, 132, 307, 543, 577
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 2 cut(s) 291, 304
Ama87I CYCGRG 1 cut(s) 521
Aor13HI TCCGGA 1 cut(s) 453
AoxI GGCC 2 cut(s) 182, 221
ApeKI GCWGC 2 cut(s) 8, 307
AspLEI GCGC 1 cut(s) 328
AsuHPI GGTGA 2 cut(s) 41, 359
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 521
BamHI GGATCC 1 cut(s) 296
BbvI GCAGC 2 cut(s) 20, 294
BccI CCATC 3 cut(s) 361, 433, 437
BceAI ACGGC 2 cut(s) 208, 256
BciT130I CCWGG 1 cut(s) 194
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 4 cut(s) 5, 129, 314, 621
BfmI CTRYAG 1 cut(s) 578
BisI GCNGC 2 cut(s) 9, 308
BlsI GCNGC 2 cut(s) 10, 309
Bme1390I CCNGG 1 cut(s) 194
BmeT110I CYCGRG 1 cut(s) 521
BmiI GGNNCC 1 cut(s) 298
BmrFI CCNGG 1 cut(s) 194
BmsI GCATC 2 cut(s) 439, 471
BmtI GCTAGC 1 cut(s) 8
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpuEI CTTGAG 1 cut(s) 583
Bsa29I ATCGAT 1 cut(s) 411
BsaBI GATNNNNATC 1 cut(s) 447
BsaWI WCCGGW 1 cut(s) 453
Bsc4I CCNNNNNNNGG 1 cut(s) 367
Bse1I ACTGG 1 cut(s) 612
Bse8I GATNNNNATC 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 453
BseBI CCWGG 1 cut(s) 194
BseCI ATCGAT 1 cut(s) 411
BseGI GGATG 2 cut(s) 88, 202
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 1 cut(s) 367
BseNI ACTGG 1 cut(s) 612
BseRI GAGGAG 1 cut(s) 24
BseXI GCAGC 2 cut(s) 20, 294
BshFI GGCC 2 cut(s) 184, 223
BshVI ATCGAT 1 cut(s) 411
BsiHKCI CYCGRG 1 cut(s) 521
BsiSI CCGG 1 cut(s) 454
BslI CCNNNNNNNGG 1 cut(s) 367
BsmAI GTCTC 1 cut(s) 524
BsnI GGCC 2 cut(s) 184, 223
BsoBI CYCGRG 1 cut(s) 521
Bsp13I TCCGGA 1 cut(s) 453
Bsp143I GATC 3 cut(s) 67, 296, 412
BspACI CCGC 3 cut(s) 26, 98, 583
BspANI GGCC 2 cut(s) 184, 223
BspDI ATCGAT 1 cut(s) 411
BspEI TCCGGA 1 cut(s) 453
BspLI GGNNCC 1 cut(s) 298
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 291, 304
BsrI ACTGG 1 cut(s) 612
BssMI GATC 3 cut(s) 67, 296, 412
BssNAI GTATAC 1 cut(s) 506
Bst1107I GTATAC 1 cut(s) 506
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 2 cut(s) 288, 536
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 6, 30, 186
BstDEI CTNAG 2 cut(s) 44, 292
BstF5I GGATG 2 cut(s) 88, 202
BstHHI GCGC 1 cut(s) 328
BstKTI GATC 3 cut(s) 70, 299, 415
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 3 cut(s) 67, 296, 412
BstMWI GCNNNNNNNGC 1 cut(s) 323
BstNI CCWGG 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 192
BstSFI CTRYAG 1 cut(s) 578
BstV1I GCAGC 2 cut(s) 20, 294
BstX2I RGATCY 1 cut(s) 296
BstYI RGATCY 1 cut(s) 296
BstZ17I GTATAC 1 cut(s) 506
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 184, 223
BsuTUI ATCGAT 1 cut(s) 411
BtgZI GCGATG 2 cut(s) 380, 411
BtsCI GGATG 2 cut(s) 88, 202
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 4 cut(s) 284, 505, 541, 619
Cac8I GCNNGC 3 cut(s) 6, 30, 186
CfoI GCGC 1 cut(s) 328
ClaI ATCGAT 1 cut(s) 411
Csp6I GTAC 2 cut(s) 15, 618
CviAII CATG 1 cut(s) 466
CviQI GTAC 2 cut(s) 15, 618
DdeI CTNAG 2 cut(s) 44, 292
DpnI GATC 3 cut(s) 69, 298, 414
DpnII GATC 3 cut(s) 67, 296, 412
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco147I AGGCCT 1 cut(s) 184
Eco88I CYCGRG 1 cut(s) 521
EcoRII CCWGG 1 cut(s) 192
FaeI CATG 1 cut(s) 469
FatI CATG 1 cut(s) 465
FauI CCCGC 2 cut(s) 33, 91
FauNDI CATATG 2 cut(s) 343, 484
FbaI TGATCA 1 cut(s) 67
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 2 cut(s) 9, 308
FokI GGATG 2 cut(s) 95, 209
Fsp4HI GCNGC 2 cut(s) 9, 308
FspBI CTAG 4 cut(s) 5, 129, 314, 621
FspI TGCGCA 1 cut(s) 327
GlaI GCGC 1 cut(s) 327
GluI GCNGC 2 cut(s) 9, 308
HaeIII GGCC 2 cut(s) 184, 223
HapII CCGG 1 cut(s) 454
HhaI GCGC 1 cut(s) 328
Hin1II CATG 1 cut(s) 469
Hin6I GCGC 1 cut(s) 326
HinP1I GCGC 1 cut(s) 326
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 2 cut(s) 116, 408
HpaII CCGG 1 cut(s) 454
HphI GGTGA 2 cut(s) 41, 359
Hpy166II GTNNAC 1 cut(s) 506
Hpy188I TCNGA 4 cut(s) 72, 155, 206, 480
Hpy188III TCNNGA 8 cut(s) 166, 247, 269, 416, 454, 523, 562, 595
Hpy8I GTNNAC 1 cut(s) 506
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 3 cut(s) 86, 149, 163
HpyCH4III ACNGT 2 cut(s) 288, 536
HpyCH4V TGCA 3 cut(s) 176, 462, 498
HpyF10VI GCNNNNNNNGC 1 cut(s) 323
HpyF3I CTNAG 2 cut(s) 44, 292
Hsp92II CATG 1 cut(s) 469
HspAI GCGC 1 cut(s) 326
Kpn2I TCCGGA 1 cut(s) 453
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 3 cut(s) 67, 296, 412
LmnI GCTCC 3 cut(s) 37, 548, 574
Lsp1109I GCAGC 2 cut(s) 20, 294
LweI GCATC 2 cut(s) 439, 471
MaeI CTAG 4 cut(s) 5, 129, 314, 621
MaeIII GTNAC 2 cut(s) 47, 275
MalI GATC 3 cut(s) 69, 298, 414
MboI GATC 3 cut(s) 67, 296, 412
MboII GAAGA 3 cut(s) 31, 127, 168
MflI RGATCY 1 cut(s) 296
MluCI AATT 4 cut(s) 209, 214, 401, 457
MnlI CCTC 5 cut(s) 45, 265, 390, 393, 591
MroI TCCGGA 1 cut(s) 453
MseI TTAA 3 cut(s) 393, 554, 606
MspA1I CMGCKG 2 cut(s) 11, 583
MspI CCGG 1 cut(s) 454
MspR9I CCNGG 1 cut(s) 194
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 323
NdeI CATATG 2 cut(s) 343, 484
NdeII GATC 3 cut(s) 67, 296, 412
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 1 cut(s) 298
NmuCI GTSAC 1 cut(s) 47
NsbI TGCGCA 1 cut(s) 327
PceI AGGCCT 1 cut(s) 184
PfeI GAWTC 2 cut(s) 116, 408
PflFI GACNNNGTC 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 192
PkrI GCNGC 2 cut(s) 10, 309
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 298
PsuI RGATCY 1 cut(s) 296
PsyI GACNNNGTC 1 cut(s) 536
PvuII CAGCTG 1 cut(s) 11
RsaI GTAC 2 cut(s) 16, 619
RsaNI GTAC 2 cut(s) 15, 618
SaqAI TTAA 3 cut(s) 393, 554, 606
SatI GCNGC 2 cut(s) 9, 308
Sau3AI GATC 3 cut(s) 67, 296, 412
ScrFI CCNGG 1 cut(s) 194
SfaNI GCATC 2 cut(s) 439, 471
SfcI CTRYAG 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 4 cut(s) 209, 214, 401, 457
SseBI AGGCCT 1 cut(s) 184
SsiI CCGC 3 cut(s) 26, 98, 583
SspMI CTAG 4 cut(s) 5, 129, 314, 621
StuI AGGCCT 1 cut(s) 184
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 2 cut(s) 288, 536
TaqI TCGA 2 cut(s) 303, 411
TasI AATT 4 cut(s) 209, 214, 401, 457
TfiI GAWTC 2 cut(s) 116, 408
Tru1I TTAA 3 cut(s) 393, 554, 606
Tru9I TTAA 3 cut(s) 393, 554, 606
TscAI CASTG 4 cut(s) 291, 505, 541, 619
TseFI GTSAC 1 cut(s) 47
TseI GCWGC 2 cut(s) 8, 307
Tsp45I GTSAC 1 cut(s) 47
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 4 cut(s) 291, 505, 541, 619
Tth111I GACNNNGTC 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 4 cut(s) 5, 129, 314, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.