Rh5AG499900

KIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000315
Physical Location & Seq
Forward (+)
84698477 .. 84703143
4667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG499900.1

Sequence Viewer

Length: 990 bp
ATGGATAACAACAATGGGAGTCCTCCCCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGTGCCAATTGCAGGTGGATTCGCGGCAAAGAATTGTCATTAAGATAATGGATACATTGAAGAGGCATATTCCTTACTCCAGTGAAGATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATGGGGCTGCCACAAGTCAGTCAGATTATCTACGGATAATTTCTCTAAAGATGCTCACTATGGAGACCGAGTCTCAGAACAGAATGGCCAATTCTTTACAATCCAACTGTGCTGGGTCTTCTGGTATACAACTCCTATCAGAGAACATTCAGAATAACATTCCACCTGCTGGAATTCAAAGTTCTACTGGCTTACCATCCGTGCTACCCCATGACTCCGGGCCACAACAGCAAACATTGCCCCCACAGCATCAACAGCAACAATTTATAGGTCTGATGGATAACAACAATGGGATTCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGAGCCAATTGCAGGCGGATTCGCGGCAAAGAATTGTCAATAAGATGATGGATACATTGAAGAGGCATCTTCCTTTCTCCGGTCAAGAGGGATTGGATGAACTCAAGAAAATTGCTGTTAGATTTGAGGAAAAGATTTATGTTGCTGCCACAAGTCAGTCAGATTATCTACGGACAATTTCTCTAAAGATGCTCACTATGGACACCAAGTCTCAGAACACAATGGCGAATTCTTTACAATCCAACTCTGCTGGGTCTGATGTTATGCAACCTCAAGTCGCCAATCAAGGACAACTCTTATCAGAGAACATTCAAAGTAACATTCCACCTGCTGGAGTTCAAAATTCTACTGGCTTATCATCTGCACTACCTCCTACCTCCGGGCCACAACAGCAAATATTGCCCCCACAACATCAACCGCAGCAATTAATTGGTTCGTATACCTCTCAATTCCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

329

Amino Acids

36.11

Weight (kDa)

5.58

Isoelectric Point (pI)

68.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 96 3e-28 KIX domain
KIX_2 PF16987 175 - 255 2.6e-34 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 67, 367, 868
Acc36I ACCTGC 3 cut(s) 67, 367, 868
AccB1I GGYRCC 1 cut(s) 66
AccB7I CCANNNNNTGG 2 cut(s) 362, 863
AccI GTMKAC 2 cut(s) 319, 971
AccII CGCG 2 cut(s) 88, 556
AciI CCGC 4 cut(s) 88, 548, 556, 950
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 3 cut(s) 366, 760, 874
AgsI TTSAA 5 cut(s) 124, 371, 592, 845, 872
AhdI GACNNNNNGTC 1 cut(s) 739
Alw26I GTCTC 3 cut(s) 251, 270, 747
AoxI GGCC 3 cut(s) 279, 413, 914
ApeKI GCWGC 3 cut(s) 200, 677, 952
ApoI RAATTY 3 cut(s) 366, 760, 874
AseI ATTAAT 1 cut(s) 959
AspS9I GGNCC 2 cut(s) 413, 914
AsuC2I CCSGG 2 cut(s) 412, 913
BalI TGGCCA 1 cut(s) 281
BanI GGYRCC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 303
BbvI GCAGC 3 cut(s) 187, 664, 964
BccI CCATC 3 cut(s) 397, 463, 574
BciVI GTATCC 2 cut(s) 109, 577
BcnI CCSGG 2 cut(s) 412, 913
BcoDI GTCTC 3 cut(s) 251, 270, 747
BfuAI ACCTGC 3 cut(s) 67, 367, 868
BfuI GTATCC 2 cut(s) 109, 577
BisI GCNGC 5 cut(s) 89, 201, 557, 678, 953
BlsI GCNGC 5 cut(s) 90, 202, 558, 679, 954
Bme1390I CCNGG 2 cut(s) 412, 913
BmeRI GACNNNNNGTC 1 cut(s) 739
BmgT120I GGNCC 2 cut(s) 413, 914
BmiI GGNNCC 2 cut(s) 68, 536
BmrFI CCNGG 2 cut(s) 412, 913
BmsI GCATC 6 cut(s) 40, 234, 451, 508, 607, 711
BpiI GAAGAC 1 cut(s) 303
BpmI CTGGAG 2 cut(s) 127, 885
BpuEI CTTGAG 4 cut(s) 143, 480, 620, 789
BpuMI CCSGG 2 cut(s) 412, 913
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 1 cut(s) 28
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bse1I ACTGG 3 cut(s) 144, 385, 886
Bse3DI GCAATG 3 cut(s) 51, 428, 519
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 4 cut(s) 55, 389, 523, 634
BseMI GCAATG 3 cut(s) 51, 428, 519
BseMII CTCAG 2 cut(s) 281, 758
BseNI ACTGG 3 cut(s) 144, 385, 886
BseRI GAGGAG 2 cut(s) 483, 547
BseXI GCAGC 3 cut(s) 187, 664, 964
BseYI CCCAGC 2 cut(s) 305, 782
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 2 cut(s) 88, 556
BshFI GGCC 3 cut(s) 281, 415, 916
BshNI GGYRCC 1 cut(s) 66
BsiSI CCGG 3 cut(s) 411, 612, 912
BsmAI GTCTC 3 cut(s) 251, 270, 747
BsnI GGCC 3 cut(s) 281, 415, 916
Bso31I GGTCTC 1 cut(s) 251
BspACI CCGC 4 cut(s) 88, 548, 556, 950
BspANI GGCC 3 cut(s) 281, 415, 916
BspCNI CTCAG 2 cut(s) 280, 757
BspFNI CGCG 2 cut(s) 88, 556
BspLI GGNNCC 2 cut(s) 68, 536
BspMI ACCTGC 3 cut(s) 67, 367, 868
BspT107I GGYRCC 1 cut(s) 66
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 3 cut(s) 51, 428, 519
BsrI ACTGG 3 cut(s) 144, 385, 886
BssECI CCNNGG 1 cut(s) 28
BssNAI GTATAC 2 cut(s) 320, 972
BssT1I CCWWGG 1 cut(s) 28
Bst1107I GTATAC 2 cut(s) 320, 972
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 2 cut(s) 119, 587
BstAPI GCANNNNNTGC 4 cut(s) 50, 430, 518, 931
BstC8I GCNNGC 3 cut(s) 42, 510, 546
BstDEI CTNAG 2 cut(s) 267, 744
BstENI CCTNNNNNAGG 2 cut(s) 27, 495
BstF5I GGATG 4 cut(s) 55, 389, 523, 634
BstFNI CGCG 2 cut(s) 88, 556
BstMAI GTCTC 3 cut(s) 251, 270, 747
BstMWI GCNNNNNNNGC 8 cut(s) 50, 421, 430, 439, 448, 518, 922, 931
BstSCI CCNGG 2 cut(s) 410, 911
BstUI CGCG 2 cut(s) 88, 556
BstV1I GCAGC 3 cut(s) 187, 664, 964
BstV2I GAAGAC 1 cut(s) 303
BstZ17I GTATAC 2 cut(s) 320, 972
BsuI GTATCC 2 cut(s) 109, 577
BsuRI GGCC 3 cut(s) 281, 415, 916
BtsCI GGATG 4 cut(s) 55, 389, 523, 634
BtsIMutI CAGTG 1 cut(s) 151
BveI ACCTGC 3 cut(s) 67, 367, 868
Cac8I GCNNGC 3 cut(s) 42, 510, 546
Cfr13I GGNCC 2 cut(s) 413, 914
CviAII CATG 1 cut(s) 404
CviJI RGCY 9 cut(s) 40, 200, 281, 384, 415, 508, 537, 885, 916
CviKI_1 RGCY 9 cut(s) 40, 200, 281, 384, 415, 508, 537, 885, 916
DdeI CTNAG 2 cut(s) 267, 744
DriI GACNNNNNGTC 1 cut(s) 739
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 2 cut(s) 119, 587
Eam1105I GACNNNNNGTC 1 cut(s) 739
EarI CTCTTC 2 cut(s) 119, 587
EciI GGCGGA 1 cut(s) 563
Eco130I CCWWGG 1 cut(s) 28
Eco31I GGTCTC 1 cut(s) 251
EcoNI CCTNNNNNAGG 2 cut(s) 27, 495
EcoRI GAATTC 2 cut(s) 366, 760
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 1 cut(s) 407
FatI CATG 1 cut(s) 403
FblI GTMKAC 2 cut(s) 319, 971
Fnu4HI GCNGC 5 cut(s) 89, 201, 557, 678, 953
FokI GGATG 4 cut(s) 62, 376, 530, 641
Fsp4HI GCNGC 5 cut(s) 89, 201, 557, 678, 953
GluI GCNGC 5 cut(s) 89, 201, 557, 678, 953
GsaI CCCAGC 2 cut(s) 309, 786
GsuI CTGGAG 2 cut(s) 127, 885
HaeIII GGCC 3 cut(s) 281, 415, 916
HapII CCGG 3 cut(s) 411, 612, 912
Hin1II CATG 1 cut(s) 407
HinfI GANTC 6 cut(s) 19, 83, 263, 407, 487, 551
HpaII CCGG 3 cut(s) 411, 612, 912
Hpy166II GTNNAC 2 cut(s) 320, 972
Hpy188I TCNGA 9 cut(s) 217, 270, 334, 345, 468, 694, 747, 790, 835
Hpy188III TCNNGA 3 cut(s) 160, 617, 637
Hpy8I GTNNAC 2 cut(s) 320, 972
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4V TGCA 8 cut(s) 44, 53, 76, 512, 521, 544, 799, 896
HpyF10VI GCNNNNNNNGC 8 cut(s) 50, 421, 430, 439, 448, 518, 922, 931
HpyF3I CTNAG 2 cut(s) 267, 744
Hsp92II CATG 1 cut(s) 407
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 3 cut(s) 187, 664, 964
LweI GCATC 6 cut(s) 40, 234, 451, 508, 607, 711
MaeIII GTNAC 1 cut(s) 848
MboII GAAGA 5 cut(s) 136, 161, 303, 593, 604
MfeI CAATTG 2 cut(s) 71, 539
MlsI TGGCCA 1 cut(s) 281
MluNI TGGCCA 1 cut(s) 281
MlyI GAGTC 3 cut(s) 28, 272, 401
MmeI TCCRAC 2 cut(s) 321, 798
Mox20I TGGCCA 1 cut(s) 281
MscI TGGCCA 1 cut(s) 281
MseI TTAA 2 cut(s) 105, 959
Msp20I TGGCCA 1 cut(s) 281
MspI CCGG 3 cut(s) 411, 612, 912
MspR9I CCNGG 2 cut(s) 412, 913
MunI CAATTG 2 cut(s) 71, 539
MvnI CGCG 2 cut(s) 88, 556
MwoI GCNNNNNNNGC 8 cut(s) 50, 421, 430, 439, 448, 518, 922, 931
NciI CCSGG 2 cut(s) 412, 913
NlaIII CATG 1 cut(s) 407
NlaIV GGNNCC 2 cut(s) 68, 536
PaqCI CACCTGC 3 cut(s) 67, 367, 868
PfeI GAWTC 3 cut(s) 83, 487, 551
PflFI GACNNNGTC 1 cut(s) 262
PflMI CCANNNNNTGG 2 cut(s) 362, 863
PkrI GCNGC 5 cut(s) 90, 202, 558, 679, 954
PleI GAGTC 3 cut(s) 27, 271, 401
PpsI GAGTC 3 cut(s) 27, 271, 401
PshBI ATTAAT 1 cut(s) 959
PspFI CCCAGC 2 cut(s) 305, 782
PspN4I GGNNCC 2 cut(s) 68, 536
PspPI GGNCC 2 cut(s) 413, 914
PsyI GACNNNGTC 1 cut(s) 262
SaqAI TTAA 2 cut(s) 105, 959
SatI GCNGC 5 cut(s) 89, 201, 557, 678, 953
Sau96I GGNCC 2 cut(s) 413, 914
SchI GAGTC 3 cut(s) 28, 272, 401
ScrFI CCNGG 2 cut(s) 412, 913
SfaNI GCATC 6 cut(s) 40, 234, 451, 508, 607, 711
SmlI CTYRAG 4 cut(s) 158, 495, 635, 804
SmoI CTYRAG 4 cut(s) 158, 495, 635, 804
SsiI CCGC 4 cut(s) 88, 548, 556, 950
SspI AATATT 1 cut(s) 930
StyD4I CCNGG 2 cut(s) 410, 911
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 1 cut(s) 302
TaqII GACCGA 1 cut(s) 275
TauI GCSGC 2 cut(s) 91, 559
TfiI GAWTC 3 cut(s) 83, 487, 551
Tru1I TTAA 2 cut(s) 105, 959
Tru9I TTAA 2 cut(s) 105, 959
TscAI CASTG 1 cut(s) 151
TseI GCWGC 3 cut(s) 200, 677, 952
TspDTI ATGAA 2 cut(s) 168, 645
TspGWI ACGGA 3 cut(s) 241, 382, 718
TspRI CASTG 1 cut(s) 151
Tth111I GACNNNGTC 1 cut(s) 262
Van91I CCANNNNNTGG 2 cut(s) 362, 863
VspI ATTAAT 1 cut(s) 959
XagI CCTNNNNNAGG 2 cut(s) 27, 495
XapI RAATTY 3 cut(s) 366, 760, 874
XmiI GTMKAC 2 cut(s) 319, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.