Rh2DG257500

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000315
Physical Location & Seq
Reverse (-)
27409303 .. 27409638
336 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG257500.1

Sequence Viewer

Length: 240 bp
ATGGATACAAACAGTGGGAGTCCTCCTCAAGGTGGAGAGCCTGCAATGGATGCAGGAGATTGGAGGTGCCAATTGCAGGTGGATTCGCGGCAAAGAATTGTCATTAAGATAATGGATACATTGAAGAGGCATATTCCTTACTCTAGTGAAGATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATGTGGCTGCCACAAGTCAGGTACATATTCTGTATGCAGTGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

79

Amino Acids

8.93

Weight (kDa)

6.05

Isoelectric Point (pI)

54.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 72 5.6e-18 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 67
Acc36I ACCTGC 1 cut(s) 67
AccB1I GGYRCC 1 cut(s) 66
AccII CGCG 1 cut(s) 88
AciI CCGC 1 cut(s) 88
AfaI GTAC 1 cut(s) 216
AfiI CCNNNNNNNGG 3 cut(s) 29, 32, 76
AgsI TTSAA 1 cut(s) 124
ApeKI GCWGC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 66
BbvI GCAGC 1 cut(s) 187
BciVI GTATCC 1 cut(s) 109
BfaI CTAG 1 cut(s) 144
BfuAI ACCTGC 1 cut(s) 67
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 2 cut(s) 89, 201
BlsI GCNGC 2 cut(s) 90, 202
BmiI GGNNCC 1 cut(s) 68
BmsI GCATC 1 cut(s) 40
BplI GAGNNNNNCTC 2 cut(s) 10, 42
BpuEI CTTGAG 2 cut(s) 12, 143
Bsc4I CCNNNNNNNGG 3 cut(s) 29, 32, 76
Bse3DI GCAATG 1 cut(s) 51
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 3 cut(s) 29, 32, 76
BseMI GCAATG 1 cut(s) 51
BseRI GAGGAG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 187
Bsh1236I CGCG 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 66
BslI CCNNNNNNNGG 3 cut(s) 29, 32, 76
BspACI CCGC 1 cut(s) 88
BspFNI CGCG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 68
BspMI ACCTGC 1 cut(s) 67
BspT107I GGYRCC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 51
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 42
BstDEI CTNAG 1 cut(s) 237
BstENI CCTNNNNNAGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 88
BstMWI GCNNNNNNNGC 1 cut(s) 50
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 187
BsuI GTATCC 1 cut(s) 109
BtsCI GGATG 1 cut(s) 55
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 2 cut(s) 19, 237
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 215
CviJI RGCY 2 cut(s) 40, 200
CviKI_1 RGCY 2 cut(s) 40, 200
CviQI GTAC 1 cut(s) 215
DdeI CTNAG 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 27
FaiI YATR 4 cut(s) 132, 195, 219, 228
Fnu4HI GCNGC 2 cut(s) 89, 201
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 2 cut(s) 89, 201
FspBI CTAG 1 cut(s) 144
GluI GCNGC 2 cut(s) 89, 201
HinfI GANTC 2 cut(s) 19, 83
Hpy188III TCNNGA 1 cut(s) 160
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 4 cut(s) 44, 53, 76, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 50
HpyF3I CTNAG 1 cut(s) 237
LpnPI CCDG 4 cut(s) 39, 54, 62, 197
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 1 cut(s) 40
MaeI CTAG 1 cut(s) 144
MaeIII GTNAC 1 cut(s) 232
MboII GAAGA 2 cut(s) 136, 161
MfeI CAATTG 1 cut(s) 71
MluCI AATT 3 cut(s) 71, 96, 165
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 5 cut(s) 33, 36, 57, 120, 175
MseI TTAA 1 cut(s) 105
MunI CAATTG 1 cut(s) 71
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 1 cut(s) 50
NlaIV GGNNCC 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 232
PaqCI CACCTGC 1 cut(s) 67
PfeI GAWTC 1 cut(s) 83
PkrI GCNGC 2 cut(s) 90, 202
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 68
RsaI GTAC 1 cut(s) 216
RsaNI GTAC 1 cut(s) 215
SaqAI TTAA 1 cut(s) 105
SatI GCNGC 2 cut(s) 89, 201
SchI GAGTC 1 cut(s) 28
SetI ASST 5 cut(s) 34, 68, 81, 179, 216
SfaNI GCATC 1 cut(s) 40
SgeI CNNG 9 cut(s) 41, 53, 66, 89, 99, 156, 172, 219, 224
SmlI CTYRAG 2 cut(s) 27, 158
SmoI CTYRAG 2 cut(s) 27, 158
Sse9I AATT 3 cut(s) 71, 96, 165
SsiI CCGC 1 cut(s) 88
SspMI CTAG 1 cut(s) 144
TaaI ACNGT 1 cut(s) 14
TasI AATT 3 cut(s) 71, 96, 165
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 105
Tru9I TTAA 1 cut(s) 105
TscAI CASTG 2 cut(s) 19, 237
TseFI GTSAC 1 cut(s) 232
TseI GCWGC 1 cut(s) 200
Tsp45I GTSAC 1 cut(s) 232
TspDTI ATGAA 1 cut(s) 168
TspRI CASTG 2 cut(s) 19, 237
XagI CCTNNNNNAGG 1 cut(s) 27
XspI CTAG 1 cut(s) 144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.