Rh5CG545200

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000315
Physical Location & Seq
Forward (+)
76064003 .. 76067346
3344 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG545200.1

Sequence Viewer

Length: 1140 bp
ATGGATACATTGAAGGGGCATCTTCCTTTCTCCGGTCAAGAGGGATTGGATGAACTCAAGAAAATTGCTGTTAGATTTGAGGAAAAGATTTATGTGGCTGCCACAAGTCAGTCAGATTATCTACGGAAAATTTCTCTAAAGATGCTCAATATGGACACCAAGTCTCAGAAAACAATGGCGAATTCTTTACAATCCAACTCTGCTGGGTCTTCTGTTATGCAACCTCAAGTCTCCAATCAAGGACAACTCTTATCAGAGACCATTCAAAGTAACATTCCACCTGCTGGAGTTCAAAATTCTACTGGCTTATCATCTGCACTACCTCCTACCTCCGGGCCACAACAGCAAATATTGCCCCCACAACATCAACCGCAGCAATTAATTGCATCTTTGGATTCCACAGCCCAGACTGGGAATACAAATGGAGCTGATTGGAAAGAGGAGGTCTATCAAAAGTATCAAAAGAAGATGCAGAAGCTGCATATATTTCAACATGATGTACAACGACAGAAAGAGCAGCAGCAGCAGCTGCCTGGACAATTGCAGCCCGAGCTACACCAAATCAGTGTTGTTAACGACGTCAACACGAATGCCCTGTCATCATCACAGAGTGGGATTAACATGTTGCAGGCAAACGTTATTCCACCCAGTATTCTTCAGAATCAACTCCAAATCCAGCTACTACATCAGCGACAGATGCAGCTGCAGTTGATGGAGAAGCAGCAGAGAATTCAGCAACAGCAGCAAGCAAAGCAGCAGGTGCCTGGACTGTCACAGGCACACCAACTACCACAGCTTCACCAAATCAGTGTTGTTAAAGACGTTAATGTGGATGCCTCGTCATCACAAACTGGGAGTAGTATGCTGCAGGCAAACATTGATCCCCCTCAGCCAAAATCTATTATTGTTGATAACAAGCATCTGAAGCAAGATCAGGAGCAGAAAATGTTTCAGAATCAACTGAAGCAACCATATCAGCAGCAATGGATGCAGAAGCAGCAGATGCTTCAAGAAATGCAACAGCTGCAGATACAAAAACTACAACAGCAAGCAAAGCAGCAGCTGCATCATATTTATGTTATATATCTCATCTTTCTCACCATTCTGTTGGGTGCTGCCTTTTTCGGGACTGGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

379

Amino Acids

42.86

Weight (kDa)

9.14

Isoelectric Point (pI)

62.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 1 - 61 3.2e-22 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 289, 748
AatII GACGTC 1 cut(s) 582
Acc36I ACCTGC 2 cut(s) 289, 748
AccB1I GGYRCC 1 cut(s) 760
AccB7I CCANNNNNTGG 1 cut(s) 284
AciI CCGC 1 cut(s) 371
AclI AACGTT 1 cut(s) 636
AclWI GGATC 1 cut(s) 875
AcsI RAATTY 4 cut(s) 129, 181, 295, 729
AcuI CTGAAG 3 cut(s) 641, 944, 983
AcyI GRCGYC 1 cut(s) 579
AdeI CACNNNGTG 1 cut(s) 611
AfaI GTAC 1 cut(s) 501
AfiI CCNNNNNNNGG 5 cut(s) 32, 284, 332, 411, 1125
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 5 cut(s) 13, 266, 293, 491, 1010
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 2 cut(s) 532, 763
AluBI AGCT 9 cut(s) 428, 478, 529, 553, 679, 703, 796, 1024, 1063
AluI AGCT 9 cut(s) 428, 478, 529, 553, 679, 703, 796, 1024, 1063
Alw26I GTCTC 3 cut(s) 168, 235, 251
AlwI GGATC 1 cut(s) 875
AlwNI CAGNNNCTG 3 cut(s) 478, 529, 1063
Ama87I CYCGRG 1 cut(s) 548
AoxI GGCC 1 cut(s) 335
ApoI RAATTY 4 cut(s) 129, 181, 295, 729
ArsI GACNNNNNNTTYG 2 cut(s) 581, 613
AseI ATTAAT 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 334
AsuHPI GGTGA 2 cut(s) 791, 1090
AvaI CYCGRG 1 cut(s) 548
BanI GGYRCC 1 cut(s) 760
BbsI GAAGAC 1 cut(s) 201
BbvCI CCTCAGC 1 cut(s) 888
BccI CCATC 1 cut(s) 706
BciT130I CCWGG 2 cut(s) 534, 765
BcnI CCSGG 1 cut(s) 334
BcoDI GTCTC 3 cut(s) 168, 235, 251
BfmI CTRYAG 3 cut(s) 704, 866, 1025
BfuAI ACCTGC 2 cut(s) 289, 748
Bme1390I CCNGG 3 cut(s) 334, 534, 765
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 1 cut(s) 548
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 1 cut(s) 762
BmrFI CCNGG 3 cut(s) 334, 534, 765
BmrI ACTGGG 3 cut(s) 420, 642, 861
BmuI ACTGGG 3 cut(s) 420, 642, 861
BpiI GAAGAC 1 cut(s) 201
BpmI CTGGAG 1 cut(s) 306
Bpu10I CCTNAGC 1 cut(s) 888
BpuEI CTTGAG 2 cut(s) 41, 210
BpuMI CCSGG 1 cut(s) 334
BsaHI GRCGYC 1 cut(s) 579
BsaI GGTCTC 1 cut(s) 251
BsaWI WCCGGW 1 cut(s) 32
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 284, 332, 411, 1125
Bse1I ACTGG 5 cut(s) 307, 415, 648, 856, 1135
Bse3DI GCAATG 1 cut(s) 989
BseBI CCWGG 2 cut(s) 534, 765
BseGI GGATG 3 cut(s) 55, 838, 993
BseLI CCNNNNNNNGG 5 cut(s) 32, 284, 332, 411, 1125
BseMI GCAATG 1 cut(s) 989
BseMII CTCAG 2 cut(s) 179, 902
BseNI ACTGG 5 cut(s) 307, 415, 648, 856, 1135
BseRI GAGGAG 1 cut(s) 455
BseYI CCCAGC 1 cut(s) 203
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 1 cut(s) 337
BshNI GGYRCC 1 cut(s) 760
BsiHKCI CYCGRG 1 cut(s) 548
BsiSI CCGG 2 cut(s) 33, 333
BslI CCNNNNNNNGG 5 cut(s) 32, 284, 332, 411, 1125
BsmAI GTCTC 3 cut(s) 168, 235, 251
BsmI GAATGC 1 cut(s) 595
BsnI GGCC 1 cut(s) 337
Bso31I GGTCTC 1 cut(s) 251
BsoBI CYCGRG 1 cut(s) 548
Bsp1407I TGTACA 1 cut(s) 499
Bsp143I GATC 2 cut(s) 880, 931
BspACI CCGC 1 cut(s) 371
BspANI GGCC 1 cut(s) 337
BspCNI CTCAG 2 cut(s) 178, 901
BspLI GGNNCC 1 cut(s) 762
BspMAI CTGCAG 3 cut(s) 708, 870, 1029
BspMI ACCTGC 2 cut(s) 289, 748
BspPI GGATC 1 cut(s) 875
BspT107I GGYRCC 1 cut(s) 760
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 989
BsrGI TGTACA 1 cut(s) 499
BsrI ACTGG 5 cut(s) 307, 415, 648, 856, 1135
BssMI GATC 2 cut(s) 880, 931
BssNI GRCGYC 1 cut(s) 579
Bst2UI CCWGG 2 cut(s) 534, 765
Bst4CI ACNGT 1 cut(s) 771
BstACI GRCGYC 1 cut(s) 579
BstAPI GCANNNNNTGC 8 cut(s) 352, 478, 529, 760, 988, 1003, 1024, 1063
BstAUI TGTACA 1 cut(s) 499
BstC8I GCNNGC 4 cut(s) 630, 747, 870, 1050
BstDEI CTNAG 2 cut(s) 165, 888
BstF5I GGATG 3 cut(s) 55, 838, 993
BstKTI GATC 2 cut(s) 883, 934
BstMAI GTCTC 3 cut(s) 168, 235, 251
BstMBI GATC 2 cut(s) 880, 931
BstNI CCWGG 2 cut(s) 534, 765
BstNSI RCATGY 1 cut(s) 625
BstSCI CCNGG 3 cut(s) 332, 532, 763
BstSFI CTRYAG 3 cut(s) 704, 866, 1025
BstV2I GAAGAC 1 cut(s) 201
BstXI CCANNNNNNTGG 1 cut(s) 1108
BsuRI GGCC 1 cut(s) 337
BtsCI GGATG 3 cut(s) 55, 838, 993
BtsIMutI CAGTG 2 cut(s) 571, 814
BveI ACCTGC 2 cut(s) 289, 748
Cac8I GCNNGC 4 cut(s) 630, 747, 870, 1050
CaiI CAGNNNCTG 3 cut(s) 478, 529, 1063
Cfr13I GGNCC 1 cut(s) 335
Csp6I GTAC 1 cut(s) 500
CviAII CATG 2 cut(s) 494, 622
CviQI GTAC 1 cut(s) 500
DdeI CTNAG 2 cut(s) 165, 888
DpnI GATC 2 cut(s) 882, 933
DpnII GATC 2 cut(s) 880, 931
DraIII CACNNNGTG 1 cut(s) 611
DriI GACNNNNNGTC 1 cut(s) 160
Eam1105I GACNNNNNGTC 1 cut(s) 160
Eco31I GGTCTC 1 cut(s) 251
Eco57I CTGAAG 3 cut(s) 641, 944, 983
Eco88I CYCGRG 1 cut(s) 548
EcoRI GAATTC 2 cut(s) 181, 729
EcoRII CCWGG 2 cut(s) 532, 763
FaeI CATG 2 cut(s) 497, 625
FatI CATG 2 cut(s) 493, 621
FokI GGATG 3 cut(s) 62, 845, 1000
GsaI CCCAGC 1 cut(s) 207
GsuI CTGGAG 1 cut(s) 306
HaeIII GGCC 1 cut(s) 337
HapII CCGG 2 cut(s) 33, 333
Hin1I GRCGYC 1 cut(s) 579
Hin1II CATG 2 cut(s) 497, 625
HincII GTYRAC 2 cut(s) 574, 583
HindII GTYRAC 2 cut(s) 574, 583
HinfI GANTC 3 cut(s) 395, 661, 955
HpaI GTTAAC 1 cut(s) 574
HpaII CCGG 2 cut(s) 33, 333
HphI GGTGA 2 cut(s) 791, 1090
Hpy166II GTNNAC 2 cut(s) 574, 583
Hpy188I TCNGA 6 cut(s) 115, 168, 256, 660, 924, 954
Hpy188III TCNNGA 5 cut(s) 38, 58, 935, 1010, 1126
Hpy8I GTNNAC 2 cut(s) 574, 583
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 1 cut(s) 7
HpyCH4III ACNGT 1 cut(s) 771
HpyCH4IV ACGT 3 cut(s) 579, 636, 822
HpyF3I CTNAG 2 cut(s) 165, 888
HpySE526I ACGT 3 cut(s) 579, 636, 822
Hsp92I GRCGYC 1 cut(s) 579
Hsp92II CATG 2 cut(s) 497, 625
KspAI GTTAAC 1 cut(s) 574
Kzo9I GATC 2 cut(s) 880, 931
LmnI GCTCC 2 cut(s) 425, 937
MaeII ACGT 3 cut(s) 579, 636, 822
MaeIII GTNAC 2 cut(s) 269, 771
MalI GATC 2 cut(s) 882, 933
MboI GATC 2 cut(s) 880, 931
MboII GAAGA 4 cut(s) 14, 201, 478, 647
MfeI CAATTG 1 cut(s) 539
MluCI AATT 8 cut(s) 63, 129, 181, 295, 377, 381, 539, 729
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 9 cut(s) 34, 73, 234, 333, 340, 433, 436, 847, 897
MseI TTAA 5 cut(s) 380, 573, 618, 816, 825
MslI CAYNNNNRTG 1 cut(s) 1074
MspA1I CMGCKG 4 cut(s) 529, 703, 1024, 1063
MspI CCGG 2 cut(s) 33, 333
MspR9I CCNGG 3 cut(s) 334, 534, 765
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 595
MvaI CCWGG 2 cut(s) 534, 765
NciI CCSGG 1 cut(s) 334
NdeII GATC 2 cut(s) 880, 931
NlaIII CATG 2 cut(s) 497, 625
NlaIV GGNNCC 1 cut(s) 762
NmuCI GTSAC 1 cut(s) 771
NspI RCATGY 1 cut(s) 625
PaqCI CACCTGC 2 cut(s) 289, 748
PciI ACATGT 1 cut(s) 621
PctI GAATGC 1 cut(s) 595
PfeI GAWTC 3 cut(s) 395, 661, 955
PflMI CCANNNNNTGG 1 cut(s) 284
PscI ACATGT 1 cut(s) 621
PshBI ATTAAT 1 cut(s) 380
Psp1406I AACGTT 1 cut(s) 636
Psp6I CCWGG 2 cut(s) 532, 763
PspFI CCCAGC 1 cut(s) 203
PspGI CCWGG 2 cut(s) 532, 763
PspN4I GGNNCC 1 cut(s) 762
PspPI GGNCC 1 cut(s) 335
PstI CTGCAG 3 cut(s) 708, 870, 1029
PstNI CAGNNNCTG 3 cut(s) 478, 529, 1063
PvuII CAGCTG 4 cut(s) 529, 703, 1024, 1063
RsaI GTAC 1 cut(s) 501
RsaNI GTAC 1 cut(s) 500
RseI CAYNNNNRTG 1 cut(s) 1074
SaqAI TTAA 5 cut(s) 380, 573, 618, 816, 825
Sau3AI GATC 2 cut(s) 880, 931
Sau96I GGNCC 1 cut(s) 335
ScrFI CCNGG 3 cut(s) 334, 534, 765
SfcI CTRYAG 3 cut(s) 704, 866, 1025
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 2 cut(s) 56, 225
SmoI CTYRAG 2 cut(s) 56, 225
Sse9I AATT 8 cut(s) 63, 129, 181, 295, 377, 381, 539, 729
SsiI CCGC 1 cut(s) 371
SspI AATATT 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 332, 532, 763
TaaI ACNGT 1 cut(s) 771
TaiI ACGT 3 cut(s) 582, 639, 825
TasI AATT 8 cut(s) 63, 129, 181, 295, 377, 381, 539, 729
TatI WGTACW 1 cut(s) 499
TfiI GAWTC 3 cut(s) 395, 661, 955
Tru1I TTAA 5 cut(s) 380, 573, 618, 816, 825
Tru9I TTAA 5 cut(s) 380, 573, 618, 816, 825
TscAI CASTG 2 cut(s) 571, 814
TseFI GTSAC 1 cut(s) 771
Tsp45I GTSAC 1 cut(s) 771
TspDTI ATGAA 1 cut(s) 66
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 2 cut(s) 571, 814
Van91I CCANNNNNTGG 1 cut(s) 284
VspI ATTAAT 1 cut(s) 380
XapI RAATTY 4 cut(s) 129, 181, 295, 729
XceI RCATGY 1 cut(s) 625
ZraI GACGTC 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.