MD11G1035800.v1.1

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000315
Physical Location & Seq
Reverse (-)
3129279 .. 3130624
1346 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1035800.v1.1.491

Sequence Viewer

Length: 408 bp
ATGGAATGGGAGAGGGGAAATGGAAGGGAAATTATAGGTTTTACTCCTAGGGTTGGAGTTGGTCTCAATAACCAGAGACCTGCTCAAGTGGAGGCAGGTGATTGGAGAAGCCAATTCCAGCTAGATTTACGACACAGAGTTCTTAGCAAGCAAATTGAAACGTTTAAGAGGCTCATTCCGTTCGAGGGCGAGGAGGGATTACTTGAACTCGAGAGGATTGCCGGAGGGCTTGAGGAAAAGATATATGTTGCTGCCTCAAGCCAGCCGGATTATCTATGGAAAATTTCTCTCAAGATGCTCACCATGGAGATGAAGTGTCAGACCGCACCACCCCCCCTCCAACCTGAAACGCTATCGCATCTCTGCTTGACTTTTGTAGGTACCCATTTATCAAAAATGGTCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

136

Amino Acids

15.39

Weight (kDa)

6.31

Isoelectric Point (pI)

48.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 33 - 108 4e-19 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 2 cut(s) 86, 88
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 380
AciI CCGC 1 cut(s) 324
AclI AACGTT 1 cut(s) 161
AcsI RAATTY 1 cut(s) 282
AfaI GTAC 1 cut(s) 382
AfiI CCNNNNNNNGG 2 cut(s) 53, 185
AgsI TTSAA 2 cut(s) 158, 206
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
Alw26I GTCTC 2 cut(s) 68, 70
Ama87I CYCGRG 1 cut(s) 209
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 282
Asp718I GGTACC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 47
AspS9I GGNCC 1 cut(s) 400
AsuHPI GGTGA 2 cut(s) 110, 292
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 1 cut(s) 400
AvrII CCTAGG 1 cut(s) 47
BanI GGYRCC 1 cut(s) 380
BbvI GCAGC 1 cut(s) 238
BcgI CGANNNNNNTGC 2 cut(s) 200, 234
BcoDI GTCTC 2 cut(s) 68, 70
BfaI CTAG 3 cut(s) 48, 122, 406
BfuAI ACCTGC 2 cut(s) 86, 88
BisI GCNGC 1 cut(s) 252
BlnI CCTAGG 1 cut(s) 47
BlsI GCNGC 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 400
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 382, 402
BmsI GCATC 2 cut(s) 285, 367
BplI GAGNNNNNCTC 4 cut(s) 48, 80, 67, 99
BpuEI CTTGAG 4 cut(s) 69, 241, 251, 275
BsaI GGTCTC 2 cut(s) 68, 70
BsaJI CCNNGG 2 cut(s) 47, 303
BsaXI ACNNNNNCTCC 4 cut(s) 299, 321, 329, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 185
BseDI CCNNGG 2 cut(s) 47, 303
BseLI CCNNNNNNNGG 2 cut(s) 53, 185
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 238
BshNI GGYRCC 1 cut(s) 380
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 2 cut(s) 222, 266
BslFI GGGAC 1 cut(s) 386
BslI CCNNNNNNNGG 2 cut(s) 53, 185
BsmAI GTCTC 2 cut(s) 68, 70
BsmFI GGGAC 1 cut(s) 386
Bso31I GGTCTC 2 cut(s) 68, 70
BsoBI CYCGRG 1 cut(s) 209
Bsp19I CCATGG 1 cut(s) 303
BspACI CCGC 1 cut(s) 324
BspLI GGNNCC 2 cut(s) 382, 402
BspMI ACCTGC 2 cut(s) 86, 88
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 68, 70
BssECI CCNNGG 2 cut(s) 47, 303
BssT1I CCWWGG 2 cut(s) 47, 303
BstC8I GCNNGC 2 cut(s) 149, 263
BstDEI CTNAG 1 cut(s) 143
BstDSI CCRYGG 1 cut(s) 303
BstMAI GTCTC 2 cut(s) 68, 70
BstV1I GCAGC 1 cut(s) 238
BtgI CCRYGG 1 cut(s) 303
BveI ACCTGC 2 cut(s) 86, 88
Cac8I GCNNGC 2 cut(s) 149, 263
Cfr13I GGNCC 1 cut(s) 400
Csp6I GTAC 1 cut(s) 381
CviAII CATG 1 cut(s) 304
CviJI RGCY 6 cut(s) 111, 121, 172, 229, 261, 265
CviKI_1 RGCY 6 cut(s) 111, 121, 172, 229, 261, 265
CviQI GTAC 1 cut(s) 381
DdeI CTNAG 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 47, 303
Eco31I GGTCTC 2 cut(s) 68, 70
Eco47I GGWCC 1 cut(s) 400
Eco88I CYCGRG 1 cut(s) 209
EcoT14I CCWWGG 2 cut(s) 47, 303
ErhI CCWWGG 2 cut(s) 47, 303
FaeI CATG 1 cut(s) 307
FaiI YATR 5 cut(s) 35, 244, 246, 277, 305
FaqI GGGAC 1 cut(s) 386
FatI CATG 1 cut(s) 303
Fnu4HI GCNGC 1 cut(s) 252
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 3 cut(s) 48, 122, 406
GluI GCNGC 1 cut(s) 252
HapII CCGG 2 cut(s) 222, 266
Hin1II CATG 1 cut(s) 307
HpaII CCGG 2 cut(s) 222, 266
HphI GGTGA 2 cut(s) 110, 292
Hpy188I TCNGA 1 cut(s) 321
Hpy188III TCNNGA 2 cut(s) 211, 292
HpyAV CCTTC 1 cut(s) 18
HpyCH4IV ACGT 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 161
Hsp92II CATG 1 cut(s) 307
KpnI GGTACC 1 cut(s) 384
LpnPI CCDG 8 cut(s) 81, 86, 93, 131, 235, 275, 279, 357
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 2 cut(s) 285, 367
MaeI CTAG 3 cut(s) 48, 122, 406
MaeII ACGT 1 cut(s) 161
MluCI AATT 4 cut(s) 30, 113, 153, 282
MmeI TCCRAC 2 cut(s) 34, 364
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 308
MspI CCGG 2 cut(s) 222, 266
NcoI CCATGG 1 cut(s) 303
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 2 cut(s) 382, 402
PaeR7I CTCGAG 1 cut(s) 209
PaqCI CACCTGC 1 cut(s) 86
PkrI GCNGC 1 cut(s) 253
Psp1406I AACGTT 1 cut(s) 161
PspN4I GGNNCC 2 cut(s) 382, 402
PspPI GGNCC 1 cut(s) 400
RsaI GTAC 1 cut(s) 382
RsaNI GTAC 1 cut(s) 381
RseI CAYNNNNRTG 1 cut(s) 308
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 1 cut(s) 252
Sau96I GGNCC 1 cut(s) 400
SetI ASST 7 cut(s) 40, 82, 100, 123, 164, 346, 382
SfaNI GCATC 2 cut(s) 285, 367
Sfr274I CTCGAG 1 cut(s) 209
SinI GGWCC 1 cut(s) 400
SlaI CTCGAG 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 308
SmlI CTYRAG 5 cut(s) 84, 209, 230, 256, 290
SmoI CTYRAG 5 cut(s) 84, 209, 230, 256, 290
Sse9I AATT 4 cut(s) 30, 113, 153, 282
SsiI CCGC 1 cut(s) 324
SspMI CTAG 3 cut(s) 48, 122, 406
StyI CCWWGG 2 cut(s) 47, 303
TaiI ACGT 1 cut(s) 164
TaqI TCGA 2 cut(s) 183, 210
TasI AATT 4 cut(s) 30, 113, 153, 282
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 326
TspGWI ACGGA 1 cut(s) 168
VpaK11BI GGWCC 1 cut(s) 400
XapI RAATTY 1 cut(s) 282
XhoI CTCGAG 1 cut(s) 209
XmaJI CCTAGG 1 cut(s) 47
XspI CTAG 3 cut(s) 48, 122, 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.