Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 468 |
| AccII | CGCG | 1 cut(s) | 1165 |
| AciI | CCGC | 4 cut(s) | 320, 541, 1154, 1298 |
| AclI | AACGTT | 1 cut(s) | 430 |
| AclWI | GGATC | 2 cut(s) | 26, 785 |
| AcsI | RAATTY | 2 cut(s) | 693, 940 |
| AcuI | CTGAAG | 2 cut(s) | 722, 1343 |
| AfaI | GTAC | 3 cut(s) | 62, 953, 980 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 468, 482, 596, 833, 836, 1217 |
| AgeI | ACCGGT | 1 cut(s) | 113 |
| AgsI | TTSAA | 6 cut(s) | 154, 577, 617, 760, 1013, 1025 |
| AjnI | CCWGG | 2 cut(s) | 1293, 1395 |
| AluBI | AGCT | 5 cut(s) | 109, 536, 747, 911, 1355 |
| AluI | AGCT | 5 cut(s) | 109, 536, 747, 911, 1355 |
| Alw21I | GWGCWC | 1 cut(s) | 913 |
| Alw26I | GTCTC | 3 cut(s) | 713, 775, 1334 |
| AlwI | GGATC | 2 cut(s) | 26, 785 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1094 |
| Ama87I | CYCGRG | 1 cut(s) | 1241 |
| AoxI | GGCC | 2 cut(s) | 65, 824 |
| ApoI | RAATTY | 2 cut(s) | 693, 940 |
| AsiGI | ACCGGT | 1 cut(s) | 113 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 272, 694 |
| AspS9I | GGNCC | 3 cut(s) | 66, 599, 842 |
| AsuHPI | GGTGA | 4 cut(s) | 193, 344, 521, 1324 |
| AvaI | CYCGRG | 1 cut(s) | 1241 |
| AvaII | GGWCC | 2 cut(s) | 599, 842 |
| BanII | GRGCYC | 2 cut(s) | 476, 913 |
| Bbv12I | GWGCWC | 1 cut(s) | 913 |
| BccI | CCATC | 4 cut(s) | 448, 853, 891, 1369 |
| BceAI | ACGGC | 4 cut(s) | 399, 862, 941, 1265 |
| BciT130I | CCWGG | 2 cut(s) | 1295, 1397 |
| BciVI | GTATCC | 1 cut(s) | 562 |
| BclI | TGATCA | 2 cut(s) | 126, 198 |
| BcoDI | GTCTC | 3 cut(s) | 713, 775, 1334 |
| BfaI | CTAG | 2 cut(s) | 6, 1411 |
| BfuI | GTATCC | 1 cut(s) | 562 |
| BmcAI | AGTACT | 1 cut(s) | 953 |
| Bme1390I | CCNGG | 2 cut(s) | 1295, 1397 |
| Bme18I | GGWCC | 2 cut(s) | 599, 842 |
| BmeT110I | CYCGRG | 1 cut(s) | 1241 |
| BmgT120I | GGNCC | 3 cut(s) | 66, 599, 842 |
| BmiI | GGNNCC | 4 cut(s) | 68, 473, 521, 1116 |
| BmrFI | CCNGG | 2 cut(s) | 1295, 1397 |
| BmsI | GCATC | 9 cut(s) | 166, 328, 448, 592, 696, 874, 1100, 1132, 1317 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 463, 495 |
| BpmI | CTGGAG | 2 cut(s) | 35, 605 |
| BpuEI | CTTGAG | 5 cut(s) | 299, 465, 816, 1273, 1283 |
| BsaI | GGTCTC | 1 cut(s) | 713 |
| BsaJI | CCNNGG | 3 cut(s) | 35, 1335, 1395 |
| BsaWI | WCCGGW | 2 cut(s) | 113, 203 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 603, 633, 710, 740, 987, 990, 1017, 1020 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 468, 482, 596, 833, 836, 1217 |
| Bse118I | RCCGGY | 1 cut(s) | 113 |
| Bse1I | ACTGG | 2 cut(s) | 351, 810 |
| Bse3DI | GCAATG | 2 cut(s) | 334, 1118 |
| BseBI | CCWGG | 2 cut(s) | 1295, 1397 |
| BseDI | CCNNGG | 3 cut(s) | 35, 1335, 1395 |
| BseGI | GGATG | 3 cut(s) | 463, 902, 1361 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 468, 482, 596, 833, 836, 1217 |
| BseMI | GCAATG | 2 cut(s) | 334, 1118 |
| BseMII | CTCAG | 5 cut(s) | 777, 959, 1079, 1094, 1134 |
| BseNI | ACTGG | 2 cut(s) | 351, 810 |
| BseRI | GAGGAG | 5 cut(s) | 501, 532, 819, 995, 1280 |
| Bsh1236I | CGCG | 1 cut(s) | 1165 |
| BshFI | GGCC | 2 cut(s) | 67, 826 |
| BshTI | ACCGGT | 1 cut(s) | 113 |
| BsiHKAI | GWGCWC | 1 cut(s) | 913 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1241 |
| BsiSI | CCGG | 2 cut(s) | 114, 204 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 468, 482, 596, 833, 836, 1217 |
| BsmAI | GTCTC | 3 cut(s) | 713, 775, 1334 |
| BsmBI | CGTCTC | 1 cut(s) | 1334 |
| BsnI | GGCC | 2 cut(s) | 67, 826 |
| Bso31I | GGTCTC | 1 cut(s) | 713 |
| BsoBI | CYCGRG | 1 cut(s) | 1241 |
| Bsp1286I | GDGCHC | 2 cut(s) | 476, 913 |
| Bsp143I | GATC | 4 cut(s) | 31, 126, 198, 790 |
| Bsp19I | CCATGG | 2 cut(s) | 35, 1335 |
| Bsp68I | TCGCGA | 1 cut(s) | 1165 |
| BspACI | CCGC | 4 cut(s) | 320, 541, 1154, 1298 |
| BspANI | GGCC | 2 cut(s) | 67, 826 |
| BspCNI | CTCAG | 5 cut(s) | 776, 958, 1078, 1093, 1135 |
| BspFNI | CGCG | 1 cut(s) | 1165 |
| BspLI | GGNNCC | 4 cut(s) | 68, 473, 521, 1116 |
| BspPI | GGATC | 2 cut(s) | 26, 785 |
| BspTNI | GGTCTC | 1 cut(s) | 713 |
| BsrDI | GCAATG | 2 cut(s) | 334, 1118 |
| BsrFI | RCCGGY | 1 cut(s) | 113 |
| BsrI | ACTGG | 2 cut(s) | 351, 810 |
| BssAI | RCCGGY | 1 cut(s) | 113 |
| BssECI | CCNNGG | 3 cut(s) | 35, 1335, 1395 |
| BssMI | GATC | 4 cut(s) | 31, 126, 198, 790 |
| BssT1I | CCWWGG | 2 cut(s) | 35, 1335 |
| Bst2UI | CCWGG | 2 cut(s) | 1295, 1397 |
| Bst4CI | ACNGT | 3 cut(s) | 495, 990, 1373 |
| Bst6I | CTCTTC | 2 cut(s) | 278, 572 |
| BstC8I | GCNNGC | 3 cut(s) | 325, 534, 769 |
| BstDEI | CTNAG | 5 cut(s) | 763, 945, 1065, 1080, 1143 |
| BstDSI | CCRYGG | 2 cut(s) | 35, 1335 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 480, 831 |
| BstF5I | GGATG | 3 cut(s) | 463, 902, 1361 |
| BstFNI | CGCG | 1 cut(s) | 1165 |
| BstKTI | GATC | 4 cut(s) | 34, 129, 201, 793 |
| BstMAI | GTCTC | 3 cut(s) | 713, 775, 1334 |
| BstMBI | GATC | 4 cut(s) | 31, 126, 198, 790 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 538, 928, 1036, 1153, 1162 |
| BstNI | CCWGG | 2 cut(s) | 1295, 1397 |
| BstSCI | CCNGG | 2 cut(s) | 1293, 1395 |
| BstUI | CGCG | 1 cut(s) | 1165 |
| BstX2I | RGATCY | 1 cut(s) | 31 |
| BstYI | RGATCY | 1 cut(s) | 31 |
| BsuI | GTATCC | 1 cut(s) | 562 |
| BsuRI | GGCC | 2 cut(s) | 67, 826 |
| BtgI | CCRYGG | 2 cut(s) | 35, 1335 |
| BtsCI | GGATG | 3 cut(s) | 463, 902, 1361 |
| BtuMI | TCGCGA | 1 cut(s) | 1165 |
| Cac8I | GCNNGC | 3 cut(s) | 325, 534, 769 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1094 |
| Cfr10I | RCCGGY | 1 cut(s) | 113 |
| Cfr13I | GGNCC | 3 cut(s) | 66, 599, 842 |
| CseI | GACGC | 1 cut(s) | 1351 |
| Csp6I | GTAC | 3 cut(s) | 61, 952, 979 |
| CspAI | ACCGGT | 1 cut(s) | 113 |
| CviAII | CATG | 5 cut(s) | 36, 79, 130, 893, 1336 |
| CviQI | GTAC | 3 cut(s) | 61, 952, 979 |
| DdeI | CTNAG | 5 cut(s) | 763, 945, 1065, 1080, 1143 |
| DpnI | GATC | 4 cut(s) | 33, 128, 200, 792 |
| DpnII | GATC | 4 cut(s) | 31, 126, 198, 790 |
| Eam1104I | CTCTTC | 2 cut(s) | 278, 572 |
| EarI | CTCTTC | 2 cut(s) | 278, 572 |
| EciI | GGCGGA | 1 cut(s) | 1313 |
| Ecl136II | GAGCTC | 1 cut(s) | 911 |
| Eco130I | CCWWGG | 2 cut(s) | 35, 1335 |
| Eco24I | GRGCYC | 2 cut(s) | 476, 913 |
| Eco31I | GGTCTC | 1 cut(s) | 713 |
| Eco47I | GGWCC | 2 cut(s) | 599, 842 |
| Eco53kI | GAGCTC | 1 cut(s) | 911 |
| Eco57I | CTGAAG | 2 cut(s) | 722, 1343 |
| Eco88I | CYCGRG | 1 cut(s) | 1241 |
| EcoICRI | GAGCTC | 1 cut(s) | 911 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 480, 831 |
| EcoO109I | RGGNCCY | 1 cut(s) | 66 |
| EcoRII | CCWGG | 2 cut(s) | 1293, 1395 |
| EcoT14I | CCWWGG | 2 cut(s) | 35, 1335 |
| EcoT38I | GRGCYC | 2 cut(s) | 476, 913 |
| ErhI | CCWWGG | 2 cut(s) | 35, 1335 |
| Esp3I | CGTCTC | 1 cut(s) | 1334 |
| FaeI | CATG | 5 cut(s) | 39, 82, 133, 896, 1339 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 163, 195, 537, 569 |
| FatI | CATG | 5 cut(s) | 35, 78, 129, 892, 1335 |
| FbaI | TGATCA | 2 cut(s) | 126, 198 |
| FokI | GGATG | 3 cut(s) | 470, 909, 1348 |
| FriOI | GRGCYC | 2 cut(s) | 476, 913 |
| FspBI | CTAG | 2 cut(s) | 6, 1411 |
| GsuI | CTGGAG | 2 cut(s) | 35, 605 |
| HaeIII | GGCC | 2 cut(s) | 67, 826 |
| HapII | CCGG | 2 cut(s) | 114, 204 |
| HgaI | GACGC | 1 cut(s) | 1351 |
| Hin1II | CATG | 5 cut(s) | 39, 82, 133, 896, 1339 |
| HincII | GTYRAC | 1 cut(s) | 1180 |
| HindII | GTYRAC | 1 cut(s) | 1180 |
| HpaII | CCGG | 2 cut(s) | 114, 204 |
| HphI | GGTGA | 4 cut(s) | 193, 344, 521, 1324 |
| Hpy166II | GTNNAC | 1 cut(s) | 1180 |
| Hpy188I | TCNGA | 8 cut(s) | 90, 170, 679, 702, 862, 883, 1144, 1323 |
| Hpy188III | TCNNGA | 5 cut(s) | 52, 602, 622, 1164, 1243 |
| Hpy8I | GTNNAC | 1 cut(s) | 1180 |
| HpyAV | CCTTC | 5 cut(s) | 258, 486, 1019, 1116, 1149 |
| HpyCH4III | ACNGT | 3 cut(s) | 495, 990, 1373 |
| HpyCH4IV | ACGT | 1 cut(s) | 430 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 538, 928, 1036, 1153, 1162 |
| HpyF3I | CTNAG | 5 cut(s) | 763, 945, 1065, 1080, 1143 |
| HpySE526I | ACGT | 1 cut(s) | 430 |
| Hsp92II | CATG | 5 cut(s) | 39, 82, 133, 896, 1339 |
| Ksp22I | TGATCA | 2 cut(s) | 126, 198 |
| Kzo9I | GATC | 4 cut(s) | 31, 126, 198, 790 |
| LmnI | GCTCC | 8 cut(s) | 54, 145, 471, 519, 908, 916, 1114, 1360 |
| LweI | GCATC | 9 cut(s) | 166, 328, 448, 592, 696, 874, 1100, 1132, 1317 |
| MaeI | CTAG | 2 cut(s) | 6, 1411 |
| MaeII | ACGT | 1 cut(s) | 430 |
| MaeIII | GTNAC | 2 cut(s) | 812, 990 |
| MalI | GATC | 4 cut(s) | 33, 128, 200, 792 |
| MboI | GATC | 4 cut(s) | 31, 126, 198, 790 |
| MfeI | CAATTG | 4 cut(s) | 159, 524, 872, 1148 |
| MflI | RGATCY | 1 cut(s) | 31 |
| MhlI | GDGCHC | 2 cut(s) | 476, 913 |
| MlyI | GAGTC | 3 cut(s) | 23, 777, 877 |
| MmeI | TCCRAC | 2 cut(s) | 397, 657 |
| MroXI | GAANNNNTTC | 2 cut(s) | 272, 694 |
| MseI | TTAA | 5 cut(s) | 213, 446, 555, 800, 1199 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1128 |
| MspI | CCGG | 2 cut(s) | 114, 204 |
| MspR9I | CCNGG | 2 cut(s) | 1295, 1397 |
| MunI | CAATTG | 4 cut(s) | 159, 524, 872, 1148 |
| MvaI | CCWGG | 2 cut(s) | 1295, 1397 |
| MvnI | CGCG | 1 cut(s) | 1165 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 538, 928, 1036, 1153, 1162 |
| NcoI | CCATGG | 2 cut(s) | 35, 1335 |
| NdeII | GATC | 4 cut(s) | 31, 126, 198, 790 |
| NlaIII | CATG | 5 cut(s) | 39, 82, 133, 896, 1339 |
| NlaIV | GGNNCC | 4 cut(s) | 68, 473, 521, 1116 |
| NruI | TCGCGA | 1 cut(s) | 1165 |
| PaeR7I | CTCGAG | 1 cut(s) | 1241 |
| PdmI | GAANNNNTTC | 2 cut(s) | 272, 694 |
| PfeI | GAWTC | 7 cut(s) | 43, 91, 1048, 1160, 1207, 1248, 1379 |
| PflFI | GACNNNGTC | 1 cut(s) | 724 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 468 |
| PinAI | ACCGGT | 1 cut(s) | 113 |
| PleI | GAGTC | 3 cut(s) | 22, 777, 876 |
| PpsI | GAGTC | 3 cut(s) | 22, 777, 876 |
| Psp124BI | GAGCTC | 1 cut(s) | 913 |
| Psp1406I | AACGTT | 1 cut(s) | 430 |
| Psp6I | CCWGG | 2 cut(s) | 1293, 1395 |
| PspGI | CCWGG | 2 cut(s) | 1293, 1395 |
| PspN4I | GGNNCC | 4 cut(s) | 68, 473, 521, 1116 |
| PspPI | GGNCC | 3 cut(s) | 66, 599, 842 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1094 |
| PsuI | RGATCY | 1 cut(s) | 31 |
| PsyI | GACNNNGTC | 1 cut(s) | 724 |
| RruI | TCGCGA | 1 cut(s) | 1165 |
| RsaI | GTAC | 3 cut(s) | 62, 953, 980 |
| RsaNI | GTAC | 3 cut(s) | 61, 952, 979 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1128 |
| SacI | GAGCTC | 1 cut(s) | 913 |
| SaqAI | TTAA | 5 cut(s) | 213, 446, 555, 800, 1199 |
| Sau3AI | GATC | 4 cut(s) | 31, 126, 198, 790 |
| Sau96I | GGNCC | 3 cut(s) | 66, 599, 842 |
| ScaI | AGTACT | 1 cut(s) | 953 |
| SchI | GAGTC | 3 cut(s) | 23, 777, 877 |
| ScrFI | CCNGG | 2 cut(s) | 1295, 1397 |
| SduI | GDGCHC | 2 cut(s) | 476, 913 |
| SfaNI | GCATC | 9 cut(s) | 166, 328, 448, 592, 696, 874, 1100, 1132, 1317 |
| Sfr274I | CTCGAG | 1 cut(s) | 1241 |
| SinI | GGWCC | 2 cut(s) | 599, 842 |
| SlaI | CTCGAG | 1 cut(s) | 1241 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1128 |
| SmlI | CTYRAG | 6 cut(s) | 278, 480, 831, 1241, 1262, 1288 |
| SmoI | CTYRAG | 6 cut(s) | 278, 480, 831, 1241, 1262, 1288 |
| SsiI | CCGC | 4 cut(s) | 320, 541, 1154, 1298 |
| SspMI | CTAG | 2 cut(s) | 6, 1411 |
| SstI | GAGCTC | 1 cut(s) | 913 |
| StyD4I | CCNGG | 2 cut(s) | 1293, 1395 |
| StyI | CCWWGG | 2 cut(s) | 35, 1335 |
| TaaI | ACNGT | 3 cut(s) | 495, 990, 1373 |
| TaiI | ACGT | 1 cut(s) | 433 |
| TaqI | TCGA | 2 cut(s) | 236, 1242 |
| TatI | WGTACW | 2 cut(s) | 60, 951 |
| TauI | GCSGC | 3 cut(s) | 323, 544, 1156 |
| TfiI | GAWTC | 7 cut(s) | 43, 91, 1048, 1160, 1207, 1248, 1379 |
| Tru1I | TTAA | 5 cut(s) | 213, 446, 555, 800, 1199 |
| Tru9I | TTAA | 5 cut(s) | 213, 446, 555, 800, 1199 |
| TspDTI | ATGAA | 2 cut(s) | 699, 818 |
| TspGWI | ACGGA | 1 cut(s) | 1200 |
| Tth111I | GACNNNGTC | 1 cut(s) | 724 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 468 |
| VpaK11BI | GGWCC | 2 cut(s) | 599, 842 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 480, 831 |
| XapI | RAATTY | 2 cut(s) | 693, 940 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1372 |
| XhoI | CTCGAG | 1 cut(s) | 1241 |
| XmnI | GAANNNNTTC | 2 cut(s) | 272, 694 |
| XspI | CTAG | 2 cut(s) | 6, 1411 |
| ZrmI | AGTACT | 1 cut(s) | 953 |