MD03G1031700.v1.1

KIX domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000315
Physical Location & Seq
Reverse (-)
2548269 .. 2552400
4132 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1031700.v1.1.491

Sequence Viewer

Length: 1179 bp
ATGGAGGCAGGTGATGATTGGAGGACCCAATTACAGTCAGAGTCACGACACAGAATTGTCGCAAAGATAATTGAAACGATGAAAAGGCACATTCCCTTTGATGGTCCGGAGGGATTACGTGAAATCGAGAGGATTGCTGTAACGTTTGAGGAAAATATGTATGTTGGTGCCTCAAGTCAGTCGGATTATTTACGGAAAATTTCTCTCAAGATGCTCACCATGGAGACCAAATCTCAGACCGCAGTCTCCCACGCATCTCTTGATCCAGTTTTGATGGATACCAATATTCAGACCAGACCTCCTCAAGGTGGAGAACCTCCAATGGAGGCAGGTGTTGATTGGAGGAGCCAATTACAGTCAGAGTCACGACACAGAATTGTCGCCAAGATAATTGAAACGATGGTGAGGCACATTCCCTTTGATGGTCCGGAGGGATTACGTGAACTCGAGAGGATTGCTGTAACATTTGAGGAAGAGATATATGTTTCTGCCTCAAGTCAGACAGATTATTTACGGAAAATTTCTATCAAGATGTTCACCATAGAGACCAAATCTCAGGTTGCAGTCTCCCACGTAAGTCTTGATCCAGTTTTGATGGATGGCAATTATCAGAGACCCCTTGAAGGTGGAGAGCCTTCAATGGAGACAGGTGATTGGAGAAGCCAATTGCAGCTAGATTCACGACGCAGAATTGTTGCCAAGATAATGGAAACATTAAAGAGGCACCTTCCTTTCAGTGGGGAGGAGGGATTACGTGAACTCGAGAAGATTGCTGCAAGGTTTGAGGAAAAGATATATGTTGCTGCCTCAAGCCAGTCGGATTATCTTCAGAAAATTTCTATGAAGATGCTCACCATGGAAAACAAGCCTCAAGGTGGAGAAACTTCAATGGTCACAAGCAATTGGAGGAGCCAATTACAGCCAGATTCACGACACAGAATTATTGCCAAGATAACGGAAGTGTTGAAGAGGTTCCTTCCCTTTAATGGTGAGGAGGGACTACGTGAACTCGAGAGGATTGCTGTAAGGTTTGAGGAAAAAATCTATACTGTTGCCCTCGGCCAGTCGGATTATCTACGGAAAATTTCTCTCAAGATGCTCGCCATGGAGAACAAGTCTCAGACCGCAGCGTCCGCAACACCCCCCTTGAACCTGAAACGGCGTCGTGTCTGTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

393

Amino Acids

44.86

Weight (kDa)

7.81

Isoelectric Point (pI)

72.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 5 - 82 1.5e-28 KIX domain
KIX_2 PF16987 112 - 187 2.2e-26 KIX domain
KIX_2 PF16987 216 - 292 3.1e-31 KIX domain
KIX_2 PF16987 300 - 379 7.2e-28 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 320
AasI GACNNNNNNGTC 1 cut(s) 1129
Acc36I ACCTGC 1 cut(s) 320
AccB1I GGYRCC 2 cut(s) 167, 723
AccIII TCCGGA 2 cut(s) 106, 427
AciI CCGC 3 cut(s) 240, 1125, 1134
AclI AACGTT 1 cut(s) 143
AclWI GGATC 2 cut(s) 257, 578
AcoI YGGCCR 1 cut(s) 1060
AcsI RAATTY 4 cut(s) 198, 519, 834, 1083
AcuI CTGAAG 1 cut(s) 812
AcyI GRCGYC 1 cut(s) 1162
AfiI CCNNNNNNNGG 8 cut(s) 101, 305, 308, 422, 623, 737, 875, 986
AgsI TTSAA 7 cut(s) 74, 395, 623, 639, 888, 967, 1150
AluBI AGCT 1 cut(s) 673
AluI AGCT 1 cut(s) 673
Alw26I GTCTC 7 cut(s) 218, 250, 539, 571, 607, 638, 1122
AlwI GGATC 2 cut(s) 257, 578
Ama87I CYCGRG 3 cut(s) 446, 761, 1010
Aor13HI TCCGGA 2 cut(s) 106, 427
AoxI GGCC 1 cut(s) 1060
ApeKI GCWGC 4 cut(s) 670, 773, 803, 1127
ApoI RAATTY 4 cut(s) 198, 519, 834, 1083
Asp700I GAANNNNTTC 1 cut(s) 971
AspS9I GGNCC 3 cut(s) 24, 104, 425
AsuHPI GGTGA 7 cut(s) 23, 208, 415, 529, 662, 844, 1001
AvaI CYCGRG 3 cut(s) 446, 761, 1010
AvaII GGWCC 3 cut(s) 24, 104, 425
BanI GGYRCC 2 cut(s) 167, 723
BbvI GCAGC 4 cut(s) 682, 760, 790, 1139
BccI CCATC 6 cut(s) 95, 268, 394, 416, 589, 593
BceAI ACGGC 1 cut(s) 1175
BcgI CGANNNNNNTGC 8 cut(s) 116, 150, 437, 471, 752, 786, 1001, 1035
BciVI GTATCC 1 cut(s) 271
BcoDI GTCTC 7 cut(s) 218, 250, 539, 571, 607, 638, 1122
BfaI CTAG 1 cut(s) 674
BfuAI ACCTGC 1 cut(s) 320
BfuI GTATCC 1 cut(s) 271
BisI GCNGC 4 cut(s) 671, 774, 804, 1128
BlsI GCNGC 4 cut(s) 672, 775, 805, 1129
Bme18I GGWCC 3 cut(s) 24, 104, 425
BmeT110I CYCGRG 3 cut(s) 446, 761, 1010
BmgT120I GGNCC 3 cut(s) 24, 104, 425
BmiI GGNNCC 6 cut(s) 26, 169, 347, 725, 911, 974
BmsI GCATC 4 cut(s) 201, 263, 837, 1086
BoxI GACNNNNGTC 1 cut(s) 242
BpuEI CTTGAG 7 cut(s) 157, 191, 288, 478, 793, 855, 1076
BsaAI YACGTR 5 cut(s) 119, 440, 574, 755, 1004
BsaHI GRCGYC 1 cut(s) 1162
BsaI GGTCTC 3 cut(s) 218, 539, 607
BsaJI CCNNGG 4 cut(s) 219, 855, 1057, 1104
BsaWI WCCGGW 2 cut(s) 106, 427
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 1100, 1130
Bsc4I CCNNNNNNNGG 8 cut(s) 101, 305, 308, 422, 623, 737, 875, 986
Bse1I ACTGG 4 cut(s) 266, 587, 814, 1063
BseAI TCCGGA 2 cut(s) 106, 427
BseDI CCNNGG 4 cut(s) 219, 855, 1057, 1104
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 8 cut(s) 101, 305, 308, 422, 623, 737, 875, 986
BseMII CTCAG 3 cut(s) 248, 569, 1133
BseNI ACTGG 4 cut(s) 266, 587, 814, 1063
BseRI GAGGAG 5 cut(s) 291, 358, 758, 922, 1007
BseXI GCAGC 4 cut(s) 682, 760, 790, 1139
BshFI GGCC 1 cut(s) 1062
BshNI GGYRCC 2 cut(s) 167, 723
BsiHKCI CYCGRG 3 cut(s) 446, 761, 1010
BsiSI CCGG 2 cut(s) 107, 428
BslFI GGGAC 1 cut(s) 1011
BslI CCNNNNNNNGG 8 cut(s) 101, 305, 308, 422, 623, 737, 875, 986
BsmAI GTCTC 7 cut(s) 218, 250, 539, 571, 607, 638, 1122
BsmFI GGGAC 1 cut(s) 1011
BsnI GGCC 1 cut(s) 1062
Bso31I GGTCTC 3 cut(s) 218, 539, 607
BsoBI CYCGRG 3 cut(s) 446, 761, 1010
Bsp13I TCCGGA 2 cut(s) 106, 427
Bsp143I GATC 2 cut(s) 262, 583
Bsp19I CCATGG 3 cut(s) 219, 855, 1104
BspACI CCGC 3 cut(s) 240, 1125, 1134
BspANI GGCC 1 cut(s) 1062
BspCNI CTCAG 3 cut(s) 247, 568, 1132
BspEI TCCGGA 2 cut(s) 106, 427
BspLI GGNNCC 6 cut(s) 26, 169, 347, 725, 911, 974
BspMI ACCTGC 1 cut(s) 320
BspPI GGATC 2 cut(s) 257, 578
BspT107I GGYRCC 2 cut(s) 167, 723
BspTNI GGTCTC 3 cut(s) 218, 539, 607
BsrI ACTGG 4 cut(s) 266, 587, 814, 1063
BssECI CCNNGG 4 cut(s) 219, 855, 1057, 1104
BssMI GATC 2 cut(s) 262, 583
BssNI GRCGYC 1 cut(s) 1162
BssT1I CCWWGG 3 cut(s) 219, 855, 1104
Bst4CI ACNGT 3 cut(s) 36, 357, 1051
Bst6I CTCTTC 2 cut(s) 468, 962
BstACI GRCGYC 1 cut(s) 1162
BstBAI YACGTR 5 cut(s) 119, 440, 574, 755, 1004
BstC8I GCNNGC 1 cut(s) 1101
BstDEI CTNAG 3 cut(s) 234, 555, 1119
BstDSI CCRYGG 3 cut(s) 219, 855, 1104
BstENI CCTNNNNNAGG 1 cut(s) 303
BstF5I GGATG 1 cut(s) 604
BstKTI GATC 2 cut(s) 265, 586
BstMAI GTCTC 7 cut(s) 218, 250, 539, 571, 607, 638, 1122
BstMBI GATC 2 cut(s) 262, 583
BstMWI GCNNNNNNNGC 1 cut(s) 1133
BstPAI GACNNNNGTC 1 cut(s) 242
BstV1I GCAGC 4 cut(s) 682, 760, 790, 1139
BstXI CCANNNNNNTGG 1 cut(s) 706
BsuI GTATCC 1 cut(s) 271
BsuRI GGCC 1 cut(s) 1062
BtgI CCRYGG 3 cut(s) 219, 855, 1104
BtsCI GGATG 1 cut(s) 604
BtsIMutI CAGTG 1 cut(s) 742
BveI ACCTGC 1 cut(s) 320
Cac8I GCNNGC 1 cut(s) 1101
Cfr13I GGNCC 3 cut(s) 24, 104, 425
CseI GACGC 3 cut(s) 693, 1119, 1151
CviAII CATG 3 cut(s) 220, 856, 1105
CviJI RGCY 9 cut(s) 348, 634, 663, 673, 813, 868, 912, 922, 1062
CviKI_1 RGCY 9 cut(s) 348, 634, 663, 673, 813, 868, 912, 922, 1062
DdeI CTNAG 3 cut(s) 234, 555, 1119
DpnI GATC 2 cut(s) 264, 585
DpnII GATC 2 cut(s) 262, 583
DrdI GACNNNNNNGTC 1 cut(s) 1129
DseDI GACNNNNNNGTC 1 cut(s) 1129
EaeI YGGCCR 1 cut(s) 1060
Eam1104I CTCTTC 2 cut(s) 468, 962
EarI CTCTTC 2 cut(s) 468, 962
Eco130I CCWWGG 3 cut(s) 219, 855, 1104
Eco31I GGTCTC 3 cut(s) 218, 539, 607
Eco47I GGWCC 3 cut(s) 24, 104, 425
Eco57I CTGAAG 1 cut(s) 812
Eco88I CYCGRG 3 cut(s) 446, 761, 1010
EcoNI CCTNNNNNAGG 1 cut(s) 303
EcoO109I RGGNCCY 1 cut(s) 24
EcoT14I CCWWGG 3 cut(s) 219, 855, 1104
ErhI CCWWGG 3 cut(s) 219, 855, 1104
FaeI CATG 3 cut(s) 223, 859, 1108
FaqI GGGAC 1 cut(s) 1011
FatI CATG 3 cut(s) 219, 855, 1104
Fnu4HI GCNGC 4 cut(s) 671, 774, 804, 1128
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 4 cut(s) 671, 774, 804, 1128
FspBI CTAG 1 cut(s) 674
GluI GCNGC 4 cut(s) 671, 774, 804, 1128
HaeIII GGCC 1 cut(s) 1062
HapII CCGG 2 cut(s) 107, 428
HgaI GACGC 3 cut(s) 693, 1119, 1151
Hin1I GRCGYC 1 cut(s) 1162
Hin1II CATG 3 cut(s) 223, 859, 1108
HinfI GANTC 4 cut(s) 41, 362, 677, 926
HpaII CCGG 2 cut(s) 107, 428
HphI GGTGA 7 cut(s) 23, 208, 415, 529, 662, 844, 1001
Hpy166II GTNNAC 4 cut(s) 443, 537, 758, 1007
Hpy8I GTNNAC 4 cut(s) 443, 537, 758, 1007
Hpy99I CGWCG 2 cut(s) 687, 1167
HpyAV CCTTC 4 cut(s) 617, 645, 737, 986
HpyCH4III ACNGT 3 cut(s) 36, 357, 1051
HpyCH4IV ACGT 6 cut(s) 118, 143, 439, 573, 754, 1003
HpyCH4V TGCA 3 cut(s) 563, 670, 776
HpyF10VI GCNNNNNNNGC 1 cut(s) 1133
HpyF3I CTNAG 3 cut(s) 234, 555, 1119
HpySE526I ACGT 6 cut(s) 118, 143, 439, 573, 754, 1003
Hsp92I GRCGYC 1 cut(s) 1162
Hsp92II CATG 3 cut(s) 223, 859, 1108
Kpn2I TCCGGA 2 cut(s) 106, 427
Kzo9I GATC 2 cut(s) 262, 583
LmnI GCTCC 2 cut(s) 345, 909
Lsp1109I GCAGC 4 cut(s) 682, 760, 790, 1139
LweI GCATC 4 cut(s) 201, 263, 837, 1086
MaeI CTAG 1 cut(s) 674
MaeII ACGT 6 cut(s) 118, 143, 439, 573, 754, 1003
MaeIII GTNAC 5 cut(s) 42, 139, 363, 460, 892
MalI GATC 2 cut(s) 264, 585
MboI GATC 2 cut(s) 262, 583
MboII GAAGA 5 cut(s) 485, 778, 818, 856, 979
MfeI CAATTG 2 cut(s) 665, 901
MlyI GAGTC 2 cut(s) 50, 371
MmeI TCCRAC 3 cut(s) 162, 798, 1047
MroI TCCGGA 2 cut(s) 106, 427
MroXI GAANNNNTTC 1 cut(s) 971
MseI TTAA 2 cut(s) 716, 984
MspI CCGG 2 cut(s) 107, 428
MunI CAATTG 2 cut(s) 665, 901
MwoI GCNNNNNNNGC 1 cut(s) 1133
NcoI CCATGG 3 cut(s) 219, 855, 1104
NdeII GATC 2 cut(s) 262, 583
NlaIII CATG 3 cut(s) 223, 859, 1108
NlaIV GGNNCC 6 cut(s) 26, 169, 347, 725, 911, 974
NmeAIII GCCGAG 1 cut(s) 1038
NmuCI GTSAC 3 cut(s) 42, 363, 892
PaeR7I CTCGAG 3 cut(s) 446, 761, 1010
PaqCI CACCTGC 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 971
PfeI GAWTC 2 cut(s) 677, 926
PkrI GCNGC 4 cut(s) 672, 775, 805, 1129
PleI GAGTC 2 cut(s) 49, 370
PpsI GAGTC 2 cut(s) 49, 370
Ppu21I YACGTR 5 cut(s) 119, 440, 574, 755, 1004
PpuMI RGGWCCY 1 cut(s) 24
PshAI GACNNNNGTC 1 cut(s) 242
Psp1406I AACGTT 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 24
PspN4I GGNNCC 6 cut(s) 26, 169, 347, 725, 911, 974
PspPI GGNCC 3 cut(s) 24, 104, 425
PspPPI RGGWCCY 1 cut(s) 24
SaqAI TTAA 2 cut(s) 716, 984
SatI GCNGC 4 cut(s) 671, 774, 804, 1128
Sau3AI GATC 2 cut(s) 262, 583
Sau96I GGNCC 3 cut(s) 24, 104, 425
SchI GAGTC 2 cut(s) 50, 371
SfaNI GCATC 4 cut(s) 201, 263, 837, 1086
Sfr274I CTCGAG 3 cut(s) 446, 761, 1010
SinI GGWCC 3 cut(s) 24, 104, 425
SlaI CTCGAG 3 cut(s) 446, 761, 1010
SsiI CCGC 3 cut(s) 240, 1125, 1134
SspI AATATT 1 cut(s) 286
SspMI CTAG 1 cut(s) 674
StyI CCWWGG 3 cut(s) 219, 855, 1104
TaaI ACNGT 3 cut(s) 36, 357, 1051
TaiI ACGT 6 cut(s) 121, 146, 442, 576, 757, 1006
TaqI TCGA 4 cut(s) 126, 447, 762, 1011
TfiI GAWTC 2 cut(s) 677, 926
Tru1I TTAA 2 cut(s) 716, 984
Tru9I TTAA 2 cut(s) 716, 984
TscAI CASTG 1 cut(s) 742
TseFI GTSAC 3 cut(s) 42, 363, 892
TseI GCWGC 4 cut(s) 670, 773, 803, 1127
Tsp45I GTSAC 3 cut(s) 42, 363, 892
TspDTI ATGAA 2 cut(s) 95, 857
TspGWI ACGGA 4 cut(s) 208, 529, 971, 1093
TspRI CASTG 1 cut(s) 742
VpaK11BI GGWCC 3 cut(s) 24, 104, 425
XagI CCTNNNNNAGG 1 cut(s) 303
XapI RAATTY 4 cut(s) 198, 519, 834, 1083
XhoI CTCGAG 3 cut(s) 446, 761, 1010
XmnI GAANNNNTTC 1 cut(s) 971
XspI CTAG 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.