Rroxscaffold_5G00343250

dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000315
Physical Location & Seq
Forward (+)
12546015 .. 12547217
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343250.1

Sequence Viewer

Length: 516 bp
ATGGATACAAACAGTGGGAGTCCTCCTCAAGGTGGAGAGCCTACAATGGATGCAGGAGATTGGAGGTGCCAATTGCAGGTGGATTCGCGGCAAAGAATTGTCATTAAGATAATGGATACATTGAAGAGGCATATTCCTTACTCTAGTGAAGATGAACTCAAGAAAATTGCTGTTAGGTTTGAGGAAAAGATTTATGTGGCTGCCACAAGTCGGGTCTTCGGTATACAACTCCTATCAAAGAACATTCGAATAACATTCCACCTGCTGGGTTTGCCTAATAAGCCTTTGGAGTTGCCTATATTTCCATTGGTTCTGGGGAGGAAGCTCGTTGGTGGAAGCGACATTGGAGGGATAAAAGAGACGCAGGAGATGCTTGACTTTTGTGCCAAACACAATATTACCTCCGAAATCGAGCTAATTAAAATGGACTACATTAACACAGCTATGGAGCGACTTGCCAAATCAGATGTCGGGTATCGGTTTGTGATCGATGTGGGGAACTCCTTGTCTCACTAG

Protein Analysis

171

Amino Acids

19.23

Weight (kDa)

6.91

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIX_2 PF16987 19 - 72 6.8e-17 KIX domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 67, 270
Acc36I ACCTGC 2 cut(s) 67, 270
AccB1I GGYRCC 1 cut(s) 66
AccB7I CCANNNNNTGG 1 cut(s) 265
AccI GTMKAC 1 cut(s) 223
AccII CGCG 1 cut(s) 88
AciI CCGC 1 cut(s) 88
AfiI CCNNNNNNNGG 5 cut(s) 29, 32, 76, 210, 265
AgsI TTSAA 1 cut(s) 124
AluBI AGCT 3 cut(s) 325, 415, 443
AluI AGCT 3 cut(s) 325, 415, 443
Alw26I GTCTC 1 cut(s) 353
ApeKI GCWGC 1 cut(s) 200
ArsI GACNNNNNNTTYG 2 cut(s) 453, 485
AsuII TTCGAA 1 cut(s) 247
BanI GGYRCC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 187
BciVI GTATCC 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 353
BfaI CTAG 2 cut(s) 144, 514
BfuAI ACCTGC 2 cut(s) 67, 270
BfuI GTATCC 1 cut(s) 109
BisI GCNGC 2 cut(s) 89, 201
BlsI GCNGC 2 cut(s) 90, 202
BmiI GGNNCC 1 cut(s) 68
BmsI GCATC 2 cut(s) 40, 360
BpiI GAAGAC 1 cut(s) 208
BplI GAGNNNNNCTC 2 cut(s) 10, 42
Bpu14I TTCGAA 1 cut(s) 247
BpuEI CTTGAG 2 cut(s) 12, 143
Bsa29I ATCGAT 1 cut(s) 489
Bsc4I CCNNNNNNNGG 5 cut(s) 29, 32, 76, 210, 265
BseCI ATCGAT 1 cut(s) 489
BseGI GGATG 1 cut(s) 55
BseLI CCNNNNNNNGG 5 cut(s) 29, 32, 76, 210, 265
BseRI GAGGAG 1 cut(s) 15
BseXI GCAGC 1 cut(s) 187
BseYI CCCAGC 1 cut(s) 265
Bsh1236I CGCG 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 66
BshVI ATCGAT 1 cut(s) 489
BslI CCNNNNNNNGG 5 cut(s) 29, 32, 76, 210, 265
BsmAI GTCTC 1 cut(s) 353
BsmBI CGTCTC 1 cut(s) 353
Bsp119I TTCGAA 1 cut(s) 247
Bsp143I GATC 1 cut(s) 486
BspACI CCGC 1 cut(s) 88
BspDI ATCGAT 1 cut(s) 489
BspFNI CGCG 1 cut(s) 88
BspLI GGNNCC 1 cut(s) 68
BspMI ACCTGC 2 cut(s) 67, 270
BspT104I TTCGAA 1 cut(s) 247
BspT107I GGYRCC 1 cut(s) 66
BssMI GATC 1 cut(s) 486
BssNAI GTATAC 1 cut(s) 224
Bst1107I GTATAC 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 14
Bst6I CTCTTC 1 cut(s) 119
BstAPI GCANNNNNTGC 1 cut(s) 370
BstBI TTCGAA 1 cut(s) 247
BstENI CCTNNNNNAGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 55
BstFNI CGCG 1 cut(s) 88
BstKTI GATC 1 cut(s) 489
BstMAI GTCTC 1 cut(s) 353
BstMBI GATC 1 cut(s) 486
BstMWI GCNNNNNNNGC 3 cut(s) 271, 280, 370
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 1 cut(s) 187
BstV2I GAAGAC 1 cut(s) 208
BstZ17I GTATAC 1 cut(s) 224
Bsu15I ATCGAT 1 cut(s) 489
BsuI GTATCC 1 cut(s) 109
BsuTUI ATCGAT 1 cut(s) 489
BtsCI GGATG 1 cut(s) 55
BtsIMutI CAGTG 1 cut(s) 19
BveI ACCTGC 2 cut(s) 67, 270
ClaI ATCGAT 1 cut(s) 489
CseI GACGC 1 cut(s) 370
CviJI RGCY 6 cut(s) 40, 200, 283, 325, 415, 443
CviKI_1 RGCY 6 cut(s) 40, 200, 283, 325, 415, 443
DpnI GATC 1 cut(s) 488
DpnII GATC 1 cut(s) 486
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
EcoNI CCTNNNNNAGG 1 cut(s) 27
Esp3I CGTCTC 1 cut(s) 353
FaiI YATR 5 cut(s) 132, 195, 224, 299, 446
FblI GTMKAC 1 cut(s) 223
Fnu4HI GCNGC 2 cut(s) 89, 201
FokI GGATG 1 cut(s) 62
Fsp4HI GCNGC 2 cut(s) 89, 201
FspBI CTAG 2 cut(s) 144, 514
GluI GCNGC 2 cut(s) 89, 201
GsaI CCCAGC 1 cut(s) 269
HgaI GACGC 1 cut(s) 370
HinfI GANTC 2 cut(s) 19, 83
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 2 cut(s) 406, 466
Hpy188III TCNNGA 1 cut(s) 160
Hpy8I GTNNAC 1 cut(s) 224
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 2 cut(s) 53, 76
HpyF10VI GCNNNNNNNGC 3 cut(s) 271, 280, 370
Kzo9I GATC 1 cut(s) 486
LmnI GCTCC 1 cut(s) 448
LpnPI CCDG 6 cut(s) 39, 62, 251, 275, 299, 350
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 2 cut(s) 40, 360
MaeI CTAG 2 cut(s) 144, 514
MalI GATC 1 cut(s) 488
MboI GATC 1 cut(s) 486
MboII GAAGA 3 cut(s) 136, 161, 208
MfeI CAATTG 1 cut(s) 71
MluCI AATT 4 cut(s) 71, 96, 165, 417
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 8 cut(s) 33, 36, 57, 120, 175, 312, 341, 412
MseI TTAA 3 cut(s) 105, 420, 435
MslI CAYNNNNRTG 1 cut(s) 443
MunI CAATTG 1 cut(s) 71
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 3 cut(s) 271, 280, 370
NdeII GATC 1 cut(s) 486
NlaIV GGNNCC 1 cut(s) 68
NspV TTCGAA 1 cut(s) 247
PaqCI CACCTGC 2 cut(s) 67, 270
PfeI GAWTC 1 cut(s) 83
PflMI CCANNNNNTGG 1 cut(s) 265
PkrI GCNGC 2 cut(s) 90, 202
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PspFI CCCAGC 1 cut(s) 265
PspN4I GGNNCC 1 cut(s) 68
RseI CAYNNNNRTG 1 cut(s) 443
SaqAI TTAA 3 cut(s) 105, 420, 435
SatI GCNGC 2 cut(s) 89, 201
Sau3AI GATC 1 cut(s) 486
SchI GAGTC 1 cut(s) 28
SetI ASST 9 cut(s) 34, 68, 81, 179, 264, 327, 404, 417, 445
SfaNI GCATC 2 cut(s) 40, 360
SfuI TTCGAA 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 443
SmlI CTYRAG 2 cut(s) 27, 158
SmoI CTYRAG 2 cut(s) 27, 158
Sse9I AATT 4 cut(s) 71, 96, 165, 417
SsiI CCGC 1 cut(s) 88
SspI AATATT 1 cut(s) 397
SspMI CTAG 2 cut(s) 144, 514
TaaI ACNGT 1 cut(s) 14
TaqI TCGA 3 cut(s) 247, 411, 489
TasI AATT 4 cut(s) 71, 96, 165, 417
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 1 cut(s) 83
Tru1I TTAA 3 cut(s) 105, 420, 435
Tru9I TTAA 3 cut(s) 105, 420, 435
TscAI CASTG 1 cut(s) 19
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 1 cut(s) 168
TspRI CASTG 1 cut(s) 19
Van91I CCANNNNNTGG 1 cut(s) 265
XagI CCTNNNNNAGG 1 cut(s) 27
XmiI GTMKAC 1 cut(s) 223
XspI CTAG 2 cut(s) 144, 514
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.