Rroxscaffold_1G00005830

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000315
Physical Location & Seq
Reverse (-)
7862263 .. 7866282
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00005830.1

Sequence Viewer

Length: 1257 bp
ATGAAGGAGATGTACTACCCCGAACTAAGTGAAATGTACCAAAAACTTGCTACTAAACTTCATCAGCTCGACTCCCTTCCACAACAGCCAAAGTCAGATCAGCTTGAGAAGCTGAAGATGTTTAAGACCATGCTGGAGCGCCTGATAACAGTCTTACAAGTTTCCAAAGCTAGCATATCACCTAGTTTGAAGGAGAAGTTGGGTTTATACGAAAAGCAGATAATAAATTTTATTAATACAAATAGACCCAGGAAGCCTGTTTCTTCTCTGCCGCCAGGGCAGCTCACCCCACCGCATATGCAGTCCATGCAGCAGTCACAATCCCAGATTACGCAAGTGCAAGCTCATGAAAATCAGATGAACCCACAATTGCAATCCCTGAACTTGCAAGGTTCTGTGCCAACAATGCAGCAGAACAATATGACGGGCTTGCAGCAAAATTCTATGTCTTCTTTATCCGGGGTTTCAACAGCACAGCAAAACATGATGAATCCGTTGCAGCCAAGTTCCAATATGGATCCAGGACAAGGAACCGCACTGAACTCACTGCAGCAGGTCCCTGTGGGCTCTATCCAACAGACTCCTGTCAGTGCTCCCCAACAAGCTAACATGAATGCCTTATCATCACAGAATGGGATTAACATGCTGCAGAGCAACATTAATCAGCTTCAGTCAAATTCTGGTATGCTTCATAGCCAACATCTGAAACAGCAGGAACAGCAATTGTATCAGAATCAACTCAAGCAACAATATCAACAACGACAGATGCAGCAGCAGCAGTTGATGCAGAAGCAGCAGATATTTCAACAACAACAACAGCAAGAGCTACAGAAGCTGCTGCCTGGACAGTTGGAACCCCAGCTTCACCAAATCAGTGTTGTTAATGACATGAACATGAATCACTTGTCATCATCAAAGAGTGGGAGTAATATGCTGCAGGAAAGCACTGATTCCCTCAAGTCAAACTCTGGTATGCTTGATAACCAGCGTCAGAAGCAAGAAACTTCCTCTGTGAACCTCAAGCACCTGTATCAACAGCTACAGATGCAGCAGCAATGGATGCAGATGCTTCAAGAAAAGCAACAGCAGCAGATACAGCAACTACAGCAGCAAGCAAAGCAGCGGCTGCATTTTATTTATTTCATCTGTCTCATCTTTCTCATCATTCTATTCGGTGCTGCTCTTTTCGGGAAGGCTATTGGATTTAATCAGGCTTTGTTCTCCCCTACCTACTATCTGTTGTTTGTAATTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

418

Amino Acids

47.88

Weight (kDa)

9.45

Isoelectric Point (pI)

64.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AciI CCGC 4 cut(s) 272, 293, 534, 1123
AclWI GGATC 2 cut(s) 512, 525
AcsI RAATTY 3 cut(s) 226, 439, 676
AcuI CTGAAG 2 cut(s) 134, 653
AfaI GTAC 2 cut(s) 14, 38
AfiI CCNNNNNNNGG 1 cut(s) 527
AgsI TTSAA 4 cut(s) 190, 468, 806, 1073
AjnI CCWGG 4 cut(s) 248, 274, 520, 841
AjuI GAANNNNNNNTTGG 2 cut(s) 182, 214
Alw21I GWGCWC 1 cut(s) 595
Alw26I GTCTC 1 cut(s) 1154
AlwI GGATC 2 cut(s) 512, 525
AlwNI CAGNNNCTG 2 cut(s) 835, 1126
ApoI RAATTY 3 cut(s) 226, 439, 676
AseI ATTAAT 2 cut(s) 234, 660
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 556
AsuC2I CCSGG 1 cut(s) 460
AsuHPI GGTGA 3 cut(s) 171, 277, 857
AsuNHI GCTAGC 1 cut(s) 170
AvaII GGWCC 1 cut(s) 556
BamHI GGATCC 1 cut(s) 517
BanII GRGCYC 1 cut(s) 569
BarI GAAGNNNNNNTAC 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 595
BciT130I CCWGG 4 cut(s) 250, 276, 522, 843
BcnI CCSGG 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 1154
BfaI CTAG 2 cut(s) 171, 183
BfmI CTRYAG 7 cut(s) 548, 647, 827, 935, 1040, 1103, 1253
BfoI RGCGCY 1 cut(s) 142
BfuAI ACCTGC 1 cut(s) 544
BglI GCCNNNNNGGC 1 cut(s) 277
Bme1390I CCNGG 5 cut(s) 250, 276, 460, 522, 843
Bme18I GGWCC 1 cut(s) 556
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 4 cut(s) 519, 532, 558, 855
BmrFI CCNGG 5 cut(s) 250, 276, 460, 522, 843
BmsI GCATC 5 cut(s) 756, 774, 1035, 1050, 1056
BmtI GCTAGC 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 584
BpiI GAAGAC 1 cut(s) 441
BpmI CTGGAG 1 cut(s) 155
BpuEI CTTGAG 4 cut(s) 125, 725, 941, 1004
BpuMI CCSGG 1 cut(s) 460
BsaJI CCNNGG 3 cut(s) 248, 275, 459
Bsc4I CCNNNNNNNGG 1 cut(s) 527
Bse3DI GCAATG 1 cut(s) 1061
BseBI CCWGG 4 cut(s) 250, 276, 522, 843
BseDI CCNNGG 3 cut(s) 248, 275, 459
BseGI GGATG 1 cut(s) 1065
BseLI CCNNNNNNNGG 1 cut(s) 527
BseMI GCAATG 1 cut(s) 1061
BseYI CCCAGC 1 cut(s) 858
BsiHKAI GWGCWC 1 cut(s) 595
BsiSI CCGG 1 cut(s) 459
BslFI GGGAC 1 cut(s) 542
BslI CCNNNNNNNGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 1154
BsmFI GGGAC 1 cut(s) 542
BsmI GAATGC 1 cut(s) 619
Bsp1286I GDGCHC 2 cut(s) 569, 595
Bsp143I GATC 2 cut(s) 97, 517
BspACI CCGC 4 cut(s) 272, 293, 534, 1123
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 4 cut(s) 519, 532, 558, 855
BspMAI CTGCAG 3 cut(s) 552, 651, 939
BspMI ACCTGC 1 cut(s) 544
BspOI GCTAGC 1 cut(s) 174
BspPI GGATC 2 cut(s) 512, 525
BsrDI GCAATG 1 cut(s) 1061
BssECI CCNNGG 3 cut(s) 248, 275, 459
BssMI GATC 2 cut(s) 97, 517
Bst2UI CCWGG 4 cut(s) 250, 276, 522, 843
Bst4CI ACNGT 2 cut(s) 151, 849
BstAPI GCANNNNNTGC 4 cut(s) 307, 784, 1060, 1126
BstC8I GCNNGC 4 cut(s) 172, 342, 431, 1113
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 1 cut(s) 1065
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 2 cut(s) 100, 520
BstMAI GTCTC 1 cut(s) 1154
BstMBI GATC 2 cut(s) 97, 517
BstNI CCWGG 4 cut(s) 250, 276, 522, 843
BstNSI RCATGY 1 cut(s) 646
BstPAI GACNNNNGTC 1 cut(s) 584
BstSCI CCNGG 5 cut(s) 248, 274, 458, 520, 841
BstSFI CTRYAG 7 cut(s) 548, 647, 827, 935, 1040, 1103, 1253
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 517
BstYI RGATCY 1 cut(s) 517
BtsCI GGATG 1 cut(s) 1065
BtsI GCAGTG 1 cut(s) 545
BtsIMutI CAGTG 5 cut(s) 536, 545, 595, 880, 945
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 172, 342, 431, 1113
CaiI CAGNNNCTG 2 cut(s) 835, 1126
CciI TCATGA 1 cut(s) 346
CfoI GCGC 1 cut(s) 141
Cfr13I GGNCC 1 cut(s) 556
CseI GACGC 1 cut(s) 977
Csp6I GTAC 2 cut(s) 13, 37
CviAII CATG 8 cut(s) 130, 307, 347, 484, 610, 643, 889, 895
CviQI GTAC 2 cut(s) 13, 37
DdeI CTNAG 1 cut(s) 26
DpnI GATC 2 cut(s) 99, 519
DpnII GATC 2 cut(s) 97, 517
Eco24I GRGCYC 1 cut(s) 569
Eco47I GGWCC 1 cut(s) 556
Eco57I CTGAAG 2 cut(s) 134, 653
EcoO109I RGGNCCY 1 cut(s) 556
EcoRII CCWGG 4 cut(s) 248, 274, 520, 841
EcoT38I GRGCYC 1 cut(s) 569
FaeI CATG 8 cut(s) 133, 310, 350, 487, 613, 646, 892, 898
FaqI GGGAC 1 cut(s) 542
FatI CATG 8 cut(s) 129, 306, 346, 483, 609, 642, 888, 894
FauNDI CATATG 1 cut(s) 297
FokI GGATG 1 cut(s) 1072
FriOI GRGCYC 1 cut(s) 569
FspBI CTAG 2 cut(s) 171, 183
GlaI GCGC 1 cut(s) 140
GsaI CCCAGC 1 cut(s) 862
GsuI CTGGAG 1 cut(s) 155
HaeII RGCGCY 1 cut(s) 142
HapII CCGG 1 cut(s) 459
HgaI GACGC 1 cut(s) 977
HhaI GCGC 1 cut(s) 141
Hin1II CATG 8 cut(s) 133, 310, 350, 487, 613, 646, 892, 898
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HinfI GANTC 6 cut(s) 71, 490, 580, 733, 898, 950
HpaII CCGG 1 cut(s) 459
HphI GGTGA 3 cut(s) 171, 277, 857
Hpy166II GTNNAC 1 cut(s) 1015
Hpy188I TCNGA 5 cut(s) 97, 357, 705, 732, 993
Hpy188III TCNNGA 3 cut(s) 347, 1073, 1189
Hpy8I GTNNAC 1 cut(s) 1015
HpyAV CCTTC 3 cut(s) 86, 184, 1186
HpyCH4III ACNGT 2 cut(s) 151, 849
HpyF3I CTNAG 1 cut(s) 26
Hsp92II CATG 8 cut(s) 133, 310, 350, 487, 613, 646, 892, 898
HspAI GCGC 1 cut(s) 139
Kzo9I GATC 2 cut(s) 97, 517
LmnI GCTCC 2 cut(s) 136, 598
LweI GCATC 5 cut(s) 756, 774, 1035, 1050, 1056
MaeI CTAG 2 cut(s) 171, 183
MaeIII GTNAC 1 cut(s) 315
MalI GATC 2 cut(s) 99, 519
MboI GATC 2 cut(s) 97, 517
MboII GAAGA 3 cut(s) 127, 255, 441
MfeI CAATTG 2 cut(s) 368, 722
MflI RGATCY 1 cut(s) 517
MhlI GDGCHC 2 cut(s) 569, 595
MluCI AATT 6 cut(s) 226, 368, 439, 676, 722, 1248
MlyI GAGTC 2 cut(s) 65, 574
MmeI TCCRAC 2 cut(s) 598, 831
MnlI CCTC 3 cut(s) 965, 1018, 1028
MseI TTAA 6 cut(s) 123, 234, 639, 660, 882, 1206
MslI CAYNNNNRTG 1 cut(s) 893
MspA1I CMGCKG 1 cut(s) 1123
MspI CCGG 1 cut(s) 459
MspR9I CCNGG 5 cut(s) 250, 276, 460, 522, 843
MunI CAATTG 2 cut(s) 368, 722
Mva1269I GAATGC 1 cut(s) 619
MvaI CCWGG 4 cut(s) 250, 276, 522, 843
NciI CCSGG 1 cut(s) 460
NdeI CATATG 1 cut(s) 297
NdeII GATC 2 cut(s) 97, 517
NheI GCTAGC 1 cut(s) 170
NlaIII CATG 8 cut(s) 133, 310, 350, 487, 613, 646, 892, 898
NlaIV GGNNCC 4 cut(s) 519, 532, 558, 855
NmuCI GTSAC 1 cut(s) 315
NspI RCATGY 1 cut(s) 646
PagI TCATGA 1 cut(s) 346
PctI GAATGC 1 cut(s) 619
PfeI GAWTC 4 cut(s) 490, 733, 898, 950
PfoI TCCNGGA 1 cut(s) 520
PleI GAGTC 2 cut(s) 65, 574
PpsI GAGTC 2 cut(s) 65, 574
PpuMI RGGWCCY 1 cut(s) 556
PshAI GACNNNNGTC 1 cut(s) 584
PshBI ATTAAT 2 cut(s) 234, 660
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 4 cut(s) 248, 274, 520, 841
PspFI CCCAGC 1 cut(s) 858
PspGI CCWGG 4 cut(s) 248, 274, 520, 841
PspN4I GGNNCC 4 cut(s) 519, 532, 558, 855
PspPI GGNCC 1 cut(s) 556
PspPPI RGGWCCY 1 cut(s) 556
PstI CTGCAG 3 cut(s) 552, 651, 939
PstNI CAGNNNCTG 2 cut(s) 835, 1126
PsuI RGATCY 1 cut(s) 517
RsaI GTAC 2 cut(s) 14, 38
RsaNI GTAC 2 cut(s) 13, 37
RseI CAYNNNNRTG 1 cut(s) 893
SaqAI TTAA 6 cut(s) 123, 234, 639, 660, 882, 1206
Sau3AI GATC 2 cut(s) 97, 517
Sau96I GGNCC 1 cut(s) 556
SchI GAGTC 2 cut(s) 65, 574
ScrFI CCNGG 5 cut(s) 250, 276, 460, 522, 843
SduI GDGCHC 2 cut(s) 569, 595
SfaNI GCATC 5 cut(s) 756, 774, 1035, 1050, 1056
SfcI CTRYAG 7 cut(s) 548, 647, 827, 935, 1040, 1103, 1253
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 893
SmlI CTYRAG 4 cut(s) 104, 740, 956, 1019
SmoI CTYRAG 4 cut(s) 104, 740, 956, 1019
Sse9I AATT 6 cut(s) 226, 368, 439, 676, 722, 1248
SsiI CCGC 4 cut(s) 272, 293, 534, 1123
SspMI CTAG 2 cut(s) 171, 183
StyD4I CCNGG 5 cut(s) 248, 274, 458, 520, 841
TaaI ACNGT 2 cut(s) 151, 849
TaqI TCGA 1 cut(s) 69
TasI AATT 6 cut(s) 226, 368, 439, 676, 722, 1248
TatI WGTACW 1 cut(s) 12
TauI GCSGC 2 cut(s) 274, 1126
TfiI GAWTC 4 cut(s) 490, 733, 898, 950
Tru1I TTAA 6 cut(s) 123, 234, 639, 660, 882, 1206
Tru9I TTAA 6 cut(s) 123, 234, 639, 660, 882, 1206
TscAI CASTG 5 cut(s) 543, 552, 595, 880, 952
TseFI GTSAC 1 cut(s) 315
Tsp45I GTSAC 1 cut(s) 315
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 5 cut(s) 543, 552, 595, 880, 952
VpaK11BI GGWCC 1 cut(s) 556
VspI ATTAAT 2 cut(s) 234, 660
XapI RAATTY 3 cut(s) 226, 439, 676
XceI RCATGY 1 cut(s) 646
XspI CTAG 2 cut(s) 171, 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.