Rorug05G0442700

Mediator of RNA polymerase II transcription subunit 15a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000315
Physical Location & Seq
Forward (+)
61464930 .. 61465429
500 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0442700.1

Sequence Viewer

Length: 312 bp
ATGTCCGGCCGCGGCAAGGGAGGCAAGGGTTTGGGCAAAGGAGGAGCCAAGCGTCACAGGAAGGTCCTCCGCGATAACATCCAGGGCATCACCAAGCCCGCCATTCGCCGTCTGGCTCGCCGTGGCGGCGTCAAGCGTATCTCCGGCCTGATCTACGAGGAGACACGTGGCGTGCTCAAGATCTTTCTCGAGAACGTCATCCGTGACGCCGTCACTTACACTGAGCACGCTCGGAGGAAGACCGTCACCGCCATGGATGTCGTCTATGCTTTGAAGAGGCAGGGTAGGACCCTTTACGGGTTCGGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
KIX_2

Protein Analysis

103

Amino Acids

11.41

Weight (kDa)

11.48

Isoelectric Point (pI)

45.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-T_C PF15511 44 - 96 3.4e-08 Centromere kinetochore component CENP-T histone fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000315)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G15790 AT1G15790 AT1G15790 AT1G15790 AT1G15790
fragaria_vesca FvH4_3g12350 FvH4_3g37483 FvH4_3g42320 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42330 FvH4_3g42361
malus_domestica MD03G1031600.v1.1 MD03G1031700.v1.1 MD03G1033500.v1.1 MD07G1261500.v1.1 MD11G1035800.v1.1
prunus_persica Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1 Prupe.6G027700_v2.0.a1
pyrus_communis pycom03g02570 pycom03g02580
rosa_chinensis RchiOBHm_Chr4g0419101 RchiOBHm_Chr5g0075711 RchiOBHm_Chr5g0075731 RchiOBHm_Chr5g0075781 RchiOBHm_Chr5g0075801 RchiOBHm_Chr5g0075811 RchiOBHm_Chr5g0075831 RchiOBHm_Chr5g0075841 RchiOBHm_Chr5g0075851 RchiOBHm_Chr5g0075861
rosa_laevigata RLG00000029834 RLG00000036588 RLG00000036591 RLG00000036592 RLG00000036594 RLG00000036595 RLG00000036597 RLG00000036647
rosa_multiflora Rmu_co8127406.1_g000001 Rmu_co8138284.1_g000001 Rmu_sc0002101.1_g000001 Rmu_sc0002101.1_g000006 Rmu_sc0002652.1_g000027 Rmu_sc0002652.1_g000029 Rmu_sc0008562.1_g000002 Rmu_sc0019599.1_g000001 Rmu_sc0019861.1_g000005 Rmu_sc0029317.1_g000001 Rmu_sc0031378.1_g000001 Rmu_ssc0000402.1_g000001
rosa_roxburghii Rroxscaffold_1G00005820 Rroxscaffold_1G00005830 Rroxscaffold_1G00005840 Rroxscaffold_1G00005850 Rroxscaffold_1G00005860 Rroxscaffold_1G00005870 Rroxscaffold_1G00005890 Rroxscaffold_5G00340930 Rroxscaffold_5G00343250 Rroxscaffold_5G00369260
rosa_rugosa Rorug05G0442200 Rorug05G0442300.1 Rorug05G0442400 Rorug05G0442500 Rorug05G0442600 Rorug05G0442700 Rorug05G0442900
rosa_samantha Rh1AG189900 Rh2DG257500 Rh5AG498900 Rh5AG499100 Rh5AG499400 Rh5AG499500 Rh5AG499600 Rh5AG499700 Rh5AG499900 Rh5AG506800 Rh5BG519900 Rh5BG520100 Rh5BG520400 Rh5BG520500 Rh5BG520600 Rh5BG520800 Rh5BG520900 Rh5BG521100 Rh5CG543900 Rh5CG544100 Rh5CG544400 Rh5CG544500 Rh5CG544600 Rh5CG544800 Rh5CG544900 Rh5CG545000 Rh5CG545200 Rh5DG532700 Rh5DG533100 Rh5DG533200 Rh5DG533400 Rh5DG533500 Rh5DG533600 Rh5DG533700
rosa_wichuraiana Rw5G046310 Rw5G046340 Rw5G046350 Rw5G046360 Rw5G046370 Rw5G046380 Rw5G046400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 12, 72
AciI CCGC 6 cut(s) 10, 12, 70, 99, 126, 249
AcoI YGGCCR 1 cut(s) 7
AcvI CACGTG 1 cut(s) 167
AcyI GRCGYC 2 cut(s) 129, 207
AfiI CCNNNNNNNGG 2 cut(s) 16, 297
AflIII ACRYGT 1 cut(s) 164
AgsI TTSAA 1 cut(s) 274
AjnI CCWGG 1 cut(s) 81
Alw21I GWGCWC 2 cut(s) 177, 228
Alw26I GTCTC 1 cut(s) 155
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 7, 145
AspS9I GGNCC 2 cut(s) 64, 288
AsuHPI GGTGA 2 cut(s) 82, 238
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 64, 288
BbrPI CACGTG 1 cut(s) 167
BbsI GAAGAC 1 cut(s) 245
Bbv12I GWGCWC 2 cut(s) 177, 228
BceAI ACGGC 3 cut(s) 93, 105, 194
BciT130I CCWGG 1 cut(s) 83
BcoDI GTCTC 1 cut(s) 155
BglI GCCNNNNNGGC 1 cut(s) 126
BglII AGATCT 1 cut(s) 180
BisI GCNGC 3 cut(s) 10, 13, 127
BlsI GCNGC 3 cut(s) 11, 14, 128
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 2 cut(s) 64, 288
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 64, 288
BmiI GGNNCC 2 cut(s) 46, 290
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 96
BpiI GAAGAC 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 167
BsaHI GRCGYC 2 cut(s) 129, 207
BsaJI CCNNGG 4 cut(s) 10, 82, 121, 252
Bsc4I CCNNNNNNNGG 2 cut(s) 16, 297
BseBI CCWGG 1 cut(s) 83
BseDI CCNNGG 4 cut(s) 10, 82, 121, 252
BseGI GGATG 3 cut(s) 78, 198, 262
BseLI CCNNNNNNNGG 2 cut(s) 16, 297
BseMII CTCAG 1 cut(s) 213
BseRI GAGGAG 2 cut(s) 57, 173
BseX3I CGGCCG 1 cut(s) 7
Bsh1236I CGCG 2 cut(s) 12, 72
Bsh1285I CGRYCG 1 cut(s) 10
BshFI GGCC 2 cut(s) 9, 147
BsiEI CGRYCG 1 cut(s) 10
BsiHKAI GWGCWC 2 cut(s) 177, 228
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 2 cut(s) 6, 144
BslI CCNNNNNNNGG 2 cut(s) 16, 297
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 9, 147
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 177, 228
Bsp143I GATC 2 cut(s) 150, 180
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 6 cut(s) 10, 12, 70, 99, 126, 249
BspANI GGCC 2 cut(s) 9, 147
BspCNI CTCAG 1 cut(s) 214
BspFNI CGCG 2 cut(s) 12, 72
BspLI GGNNCC 2 cut(s) 46, 290
BssECI CCNNGG 4 cut(s) 10, 82, 121, 252
BssMI GATC 2 cut(s) 150, 180
BssNI GRCGYC 2 cut(s) 129, 207
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 269
BstACI GRCGYC 2 cut(s) 129, 207
BstBAI YACGTR 1 cut(s) 167
BstC8I GCNNGC 4 cut(s) 99, 118, 173, 228
BstDEI CTNAG 1 cut(s) 222
BstDSI CCRYGG 3 cut(s) 10, 121, 252
BstF5I GGATG 3 cut(s) 78, 198, 262
BstFNI CGCG 2 cut(s) 12, 72
BstKTI GATC 2 cut(s) 153, 183
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 2 cut(s) 150, 180
BstMCI CGRYCG 1 cut(s) 10
BstMWI GCNNNNNNNGC 2 cut(s) 21, 126
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstUI CGCG 2 cut(s) 12, 72
BstV2I GAAGAC 1 cut(s) 245
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 1 cut(s) 7
BsuRI GGCC 2 cut(s) 9, 147
BtgI CCRYGG 3 cut(s) 10, 121, 252
BtsCI GGATG 3 cut(s) 78, 198, 262
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 4 cut(s) 99, 118, 173, 228
Cfr13I GGNCC 2 cut(s) 64, 288
Cfr42I CCGCGG 1 cut(s) 13
CseI GACGC 3 cut(s) 41, 118, 215
CviAII CATG 1 cut(s) 253
CviJI RGCY 5 cut(s) 9, 47, 97, 116, 147
CviKI_1 RGCY 5 cut(s) 9, 47, 97, 116, 147
DdeI CTNAG 1 cut(s) 222
DpnI GATC 2 cut(s) 152, 182
DpnII GATC 2 cut(s) 150, 180
EaeI YGGCCR 1 cut(s) 7
EagI CGGCCG 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
EclXI CGGCCG 1 cut(s) 7
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 2 cut(s) 64, 288
Eco52I CGGCCG 1 cut(s) 7
Eco72I CACGTG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 188
EcoO109I RGGNCCY 2 cut(s) 64, 288
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 1 cut(s) 256
FaiI YATR 2 cut(s) 254, 267
FatI CATG 1 cut(s) 252
FauI CCCGC 1 cut(s) 106
Fnu4HI GCNGC 3 cut(s) 10, 13, 127
FokI GGATG 3 cut(s) 65, 185, 269
Fsp4HI GCNGC 3 cut(s) 10, 13, 127
GluI GCNGC 3 cut(s) 10, 13, 127
HaeIII GGCC 2 cut(s) 9, 147
HapII CCGG 2 cut(s) 6, 144
HgaI GACGC 3 cut(s) 41, 118, 215
Hin1I GRCGYC 2 cut(s) 129, 207
Hin1II CATG 1 cut(s) 256
HpaII CCGG 2 cut(s) 6, 144
HphI GGTGA 2 cut(s) 82, 238
Hpy188I TCNGA 1 cut(s) 234
Hpy188III TCNNGA 3 cut(s) 178, 188, 190
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4IV ACGT 2 cut(s) 166, 195
HpyF10VI GCNNNNNNNGC 2 cut(s) 21, 126
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 2 cut(s) 166, 195
Hsp92I GRCGYC 2 cut(s) 129, 207
Hsp92II CATG 1 cut(s) 256
KspI CCGCGG 1 cut(s) 13
Kzo9I GATC 2 cut(s) 150, 180
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 8 cut(s) 19, 43, 68, 95, 98, 157, 161, 266
LweI GCATC 1 cut(s) 96
MaeII ACGT 2 cut(s) 166, 195
MaeIII GTNAC 4 cut(s) 53, 203, 211, 244
MalI GATC 2 cut(s) 152, 182
MboI GATC 2 cut(s) 150, 180
MboII GAAGA 2 cut(s) 250, 286
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 2 cut(s) 177, 228
MnlI CCTC 6 cut(s) 14, 35, 77, 151, 228, 270
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 12
MspI CCGG 2 cut(s) 6, 144
MspR9I CCNGG 1 cut(s) 83
MvaI CCWGG 1 cut(s) 83
MvnI CGCG 2 cut(s) 12, 72
MwoI GCNNNNNNNGC 2 cut(s) 21, 126
NcoI CCATGG 1 cut(s) 252
NdeII GATC 2 cut(s) 150, 180
NlaIII CATG 1 cut(s) 256
NlaIV GGNNCC 2 cut(s) 46, 290
NmuCI GTSAC 4 cut(s) 53, 203, 211, 244
PaeR7I CTCGAG 1 cut(s) 188
PflFI GACNNNGTC 1 cut(s) 209
PkrI GCNGC 3 cut(s) 11, 14, 128
PmaCI CACGTG 1 cut(s) 167
PmlI CACGTG 1 cut(s) 167
Ppu21I YACGTR 1 cut(s) 167
PpuMI RGGWCCY 2 cut(s) 64, 288
Psp5II RGGWCCY 2 cut(s) 64, 288
Psp6I CCWGG 1 cut(s) 81
PspCI CACGTG 1 cut(s) 167
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 2 cut(s) 46, 290
PspPI GGNCC 2 cut(s) 64, 288
PspPPI RGGWCCY 2 cut(s) 64, 288
PsuI RGATCY 1 cut(s) 180
PsyI GACNNNGTC 1 cut(s) 209
RseI CAYNNNNRTG 1 cut(s) 251
SacII CCGCGG 1 cut(s) 13
SatI GCNGC 3 cut(s) 10, 13, 127
Sau3AI GATC 2 cut(s) 150, 180
Sau96I GGNCC 2 cut(s) 64, 288
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 2 cut(s) 177, 228
SetI ASST 3 cut(s) 66, 169, 198
SfaNI GCATC 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 188
Sfr303I CCGCGG 1 cut(s) 13
SgrBI CCGCGG 1 cut(s) 13
SinI GGWCC 2 cut(s) 64, 288
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 2 cut(s) 176, 188
SmoI CTYRAG 2 cut(s) 176, 188
SsiI CCGC 6 cut(s) 10, 12, 70, 99, 126, 249
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 1 cut(s) 244
TaiI ACGT 2 cut(s) 169, 198
TaqI TCGA 1 cut(s) 189
TauI GCSGC 3 cut(s) 12, 15, 129
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 4 cut(s) 53, 203, 211, 244
Tsp45I GTSAC 4 cut(s) 53, 203, 211, 244
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 1 cut(s) 226
Tth111I GACNNNGTC 1 cut(s) 209
VpaK11BI GGWCC 2 cut(s) 64, 288
XcmI CCANNNNNNNNNTGG 1 cut(s) 109
XhoI CTCGAG 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.