FvH4_3g41702

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000161
Physical Location & Seq
Reverse (-)
34982816 .. 34989258
6443 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g41702.t1

Sequence Viewer

Length: 3621 bp
ATGATTGGATCTTCTCCTGTGATCCTCGGGCGTGGAACCGCTCACTTTTTTCTGCCTCATGGCACTATGATCCACGTCACGGACGCTCTCTATGCTCCAAAAGAGCATCGAACCTTGTTGAGCTTTAAGGACATTCGTGCTAATGGCTTTCACTTGGAAACTCATTGTGAAGATGGAACTGAGTTCTTATGTATTACTTCTCATGATTACGGACGTAAACGTCTATTGGAGAAACTTATGAGTCAATCTAGTGGTCTTTACCTTACTACGATTCGGATTATTGAATCTCATGTAGTCGCCAAACAGGATCTTTGGGACTCTGACTCATACAAGCTTTGGCATGATCGACTTGGTCATCCCGGAAGGGACATAATGATCCGAATTCTGTGTAATTCTCATGGACATCCCTTCTTTAGATCAAAACGAAAATATTTGGGATCTTTGCATCATAGTGGTGATGCCACAAATGGCCATCACGACCCAAAGGGGACTTTGACGTCTCCCGGGCTAGGCACCAACTTCCTGGCTGCCGCTCGGCCGACCCCTCAAGCAATTGAGGCGCGCCGTTCTTCCCTTGATGTGTCGTTGACTCTTTCCAACGCTCATCGTTCGTTTTGCCCAGCTTGCTCTTTAGCGAAAGTAGTATCGAGACCGTCTTACGCTAAAGATAATACCGAGAACATTCCATTTCTACATCGTATCCAAGGTGATATTTGTGGACCTATCCATCCTGAATGTGGACCATTTCGATACTTTATGGTCCTGGTGGATGCTTCAACGCGTTGGACTCATGTCGCGCTCTTGTCCACACGTAATGCTGCATTTCCAAAACTCCTAGCACAGATTATTAAACTTCGGGCTCACCACCCGGATTATCTAATTAAGTCAATTCGACTGGATAACGCTGGAAAATTTACATCAAAGACTTTTGATGACTATTGCATGTCTATCGGGATTGAGGTTGAACATCCCGTACCCCATGTTCACACGCAAAACGGTCTCGCCGAAGCTACAATTAAGCGGCTTCAACTCATTGCTCGAGCGTTGGTAATGCTCACCAACCTCCCTATTGCGTCATGGGGCTATGCAATATTGCATGCAGCCTTACTTATTCATTTTAGACCCACTGCTAAACAACCATTTAGTGCGTACCAGTTGGTCACTGGATATGAGCCTGACATTTCGCATCTACGCATTTTCGGTTGTTCGGTTTATGTGCCAATTACGCCTCCATTGCGTTCTAAGATGGGTCATCAGAGACGATTAGATGTCTACGTTGGTTATGAATCTCCAACGATTATCAGATACCTTGAGCCATTAACAGGTGATCTCTTTACCGCACGGTTTGCGGATTGTCACTTTGATGAGACATTCTTCCCGCCGTTAGGGGGAGATACGAACAAACGTGTTCATGTGGAACTACAGGAATTGTCGTGGTTTGTCCCAACTTTGTCTCATTATGATCCCCGCACCTCACAAACTGAAAGTGAAGTGAAACGTATTCTCGAACTTCAGAAAGTAGCAGATACGATGCCTGACTCTTTCTCCGAGATTGCGAAACTGACAAGATCAGATATACCAGCTGCAAATGCTCCTGCTAGGCTCGAAGTCCTTAGAAAGGGCACGGCCATCCCCGGGCTCGGTATTGCCGCCGCCGACGGCGCCGCCCATGGTGGGGGGATGGAGGCCGTGGCTCCCCAAAGGAAAAGGGGGAGGCCACCTGGTTCGGTTGACACACGTCCGAGAAAGAAACGGACCGACAAGGCACGAGTCAATCCTTTGATCATCGATGTTGAGAATCCTTCTCACGAGATCATCTCTGATTACAGCTATGTCCACGAATTGATGTTAGAATCCATGAGGGACGCCTCAGGGTCAATGTCAACATCAGAGAACATAGAGATCTCCATGTGTTATGATAACACTTATGAATTGATGAACCGGTCATCAACCCGTGTCGATGATATATTTGCTTATTCCGTGGCTCATGAAATCATTGAGCACGATGACATCGAACCATGCTCTGTTGCAAAATGCCAAAGCAGAGCAGATTGGCCTAAATGGAAAGTTGCAATCCAGGCAGAATTAGACTCTCTAACAAAGAGACAGGTCTTCGGCCCAGCAGTGCTAACTCCGCCGAGTGTAAAGCCTGTTGGACATAAATGGGTCTTTGTTAGAAAGCGTAATGAGAATAATGAGGTACTTCGGTATAAGACCCGCCTTGTGGCGCAAGGTTTCTCACAACGCCTTGGGATTGATTACGAAGAGATTTACTCTCCGGTCATGGACGTCATAACTTTTCGCTACTTAGTCAGTTTGGTAGTGTCCGAAAAACTTGACATGCAGCTCATGGATGTGGTTACGGCATATCTCTATGGGGATCTAGATTCAGAGATATATATGAAAGTGCCTAATGGCATTACGTTTCCTAAATCAAGTGACTCTAAACCACGGAGTGCGTATGCTATAAGGTTGCAACGCTCACTTTACGGATTAAAGCAATCCGGACGAATGTGGTATACTCGTCTAAATGACTACTTGATTAGGGAGGGATATAAGAACGATGAACTATGCCCGTGCGTGTTCATAAAGACGACAAGTTCCAGATTTGCAATAGTGGCCGTCTATGTCAATGACATGAATGTTATCGGTACTCTTGATGAAATCAAAGAGACCGCGGCTTATCTGAAATCCGAGTTTGAGATGAAAGATCTTGGGAAGACTCGATTTTGCCTAGGTCTTGAACTAGAGCACCGAGTTTGTGGAATTCTTATCCACCAGTCTGCATATGTCCAAAAGATGCTCAGGCGATTTAATATGGACAAGATGTACCCTGTTAGCACTCCCATGATTGGTCGAAGTCTGGATATATCTAAGGATCCATTCCGTCCGAAAGAGGACGACGAAAAGGTCTTGGGCGCAGAGGTTCCATACCTTAGTGCAATAGGCGCGTTATTGTACTTAGCTCAATGCACTCGACCAGACATTGCATTCTCAGTCAACTTGTTAGCTAGATTTAGCTCAGCGCCAACCCAACGTCCTTGGATCGGTGTCAAGAACATCTTTCGATACCTTAAGGGTACCATTGACTTGGGACTGTTCTTTCCCTACACTAATTCAAGGATAGAAGATGGTCCTATGGCTCATGGAAGACACGGCACTGATCCACCAAGGGACCGGATCGCCTCCATGGACGCCGCCGGCACGGCTGCTGGACACGGGACCAATAGCGAGCACGCCACCGCCGCCTTGTCCTATGCCGAGACACCAAACAATACATTGGTGGGTTTCGCCGACGCCGGATATTTATCTGATCCTCATAAAGGACGTTCTCAAACTGGTTATGTATTCACCATTGGGAATACAGCGATATCTTGGAGGTCTACAAAGCAGACTCTAGGGGCGACATCTTCAAATCATGCAGAGATTATAGCTTTGCATGAAGCTGTTCGGGAATGTTCGGAGAGTCCCTCTCTCTCTCTCTCAAAACCAACACAGAAAAGTGAGAAAACCCTAGCGCCGGGTTTAGGAGTTTGTCATGGCTTCAAAGCGGATCAGCAAGGAATTGAAGGACCTGCAGAAAGACCCGCCGGCATCCTGCAGTGCTGGTCCTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

1207

Amino Acids

134.71

Weight (kDa)

8.52

Isoelectric Point (pI)

45.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 85 - 133 7.9e-06 GAG-pre-integrase domain
rve PF00665 233 - 323 5.2e-08 Integrase core domain
RVT_2 PF07727 709 - 951 1.3e-58 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 219, 496, 2909
AatII GACGTC 2 cut(s) 500, 2292
Acc36I ACCTGC 1 cut(s) 3583
Acc65I GGTACC 1 cut(s) 3080
AccB1I GGYRCC 3 cut(s) 512, 1661, 3080
AccBSI CCGCTC 2 cut(s) 41, 533
AccI GTMKAC 3 cut(s) 1272, 2519, 3383
AccII CGCG 5 cut(s) 562, 781, 797, 2678, 2951
AccIII TCCGGA 1 cut(s) 2504
AcoI YGGCCR 4 cut(s) 469, 536, 1626, 2619
AcsI RAATTY 3 cut(s) 381, 911, 2766
AcuI CTGAAG 1 cut(s) 1496
AcyI GRCGYC 6 cut(s) 497, 1662, 1866, 2289, 3195, 3297
AdeI CACNNNGTG 1 cut(s) 2456
AfaI GTAC 7 cut(s) 975, 1151, 2202, 2653, 2831, 2960, 3082
AflII CTTAAG 1 cut(s) 3074
AflIII ACRYGT 4 cut(s) 779, 809, 1405, 1736
AgeI ACCGGT 1 cut(s) 1941
AgsI TTSAA 9 cut(s) 284, 777, 965, 1028, 2744, 3120, 3414, 3547, 3569
AjiI CACGTC 2 cut(s) 76, 1739
AjnI CCWGG 4 cut(s) 522, 762, 1720, 2076
Alw21I GWGCWC 3 cut(s) 2004, 2754, 3237
Alw26I GTCTC 9 cut(s) 504, 643, 1004, 1252, 1361, 1458, 2098, 2666, 3257
Ama87I CYCGRG 4 cut(s) 26, 503, 1038, 1634
Aor13HI TCCGGA 1 cut(s) 2504
AoxI GGCC 8 cut(s) 469, 536, 1626, 1686, 1715, 2054, 2116, 2619
ApeKI GCWGC 6 cut(s) 527, 818, 1100, 1583, 2344, 3209
ApoI RAATTY 3 cut(s) 381, 911, 2766
AscI GGCGCGCC 1 cut(s) 560
AsiGI ACCGGT 1 cut(s) 1941
Asp700I GAANNNNTTC 1 cut(s) 3447
Asp718I GGTACC 1 cut(s) 3080
AspA2I CCTAGG 1 cut(s) 2734
AspLEI GCGC 9 cut(s) 562, 564, 799, 1664, 2230, 2921, 2951, 3028, 3520
AsuC2I CCSGG 7 cut(s) 360, 504, 505, 869, 1635, 1636, 3522
AsuHPI GGTGA 6 cut(s) 467, 719, 854, 1048, 1337, 3343
AvaI CYCGRG 4 cut(s) 26, 503, 1038, 1634
AvaII GGWCC 9 cut(s) 719, 740, 760, 1755, 3134, 3175, 3222, 3572, 3609
AvrII CCTAGG 1 cut(s) 2734
AxyI CCTNAGG 1 cut(s) 1870
BaeGI GKGCMC 1 cut(s) 1625
BaeI ACNNNNGTAYC 2 cut(s) 2192, 2225
BalI TGGCCA 1 cut(s) 471
BamHI GGATCC 1 cut(s) 2878
BanI GGYRCC 3 cut(s) 512, 1661, 3080
BanII GRGCYC 2 cut(s) 862, 1641
BauI CACGAG 2 cut(s) 1767, 1808
BbsI GAAGAC 3 cut(s) 2104, 2726, 3157
Bbv12I GWGCWC 3 cut(s) 2004, 2754, 3237
BbvI GCAGC 6 cut(s) 514, 805, 1112, 1570, 2356, 3196
BccI CCATC 7 cut(s) 167, 480, 735, 1240, 1637, 1675, 3125
BceAI ACGGC 9 cut(s) 549, 1366, 1641, 1673, 1675, 2379, 2606, 3172, 3222
BcgI CGANNNNNNTGC 2 cut(s) 931, 965
BciT130I CCWGG 4 cut(s) 524, 764, 1722, 2078
BciVI GTATCC 1 cut(s) 710
BclI TGATCA 1 cut(s) 1782
BcnI CCSGG 7 cut(s) 360, 504, 505, 869, 1635, 1636, 3522
BcoDI GTCTC 9 cut(s) 504, 643, 1004, 1252, 1361, 1458, 2098, 2666, 3257
BfmI CTRYAG 3 cut(s) 1421, 3576, 3599
BfoI RGCGCY 3 cut(s) 1665, 3029, 3521
BfrI CTTAAG 1 cut(s) 3074
BfuAI ACCTGC 1 cut(s) 3583
BfuI GTATCC 1 cut(s) 710
BglI GCCNNNNNGGC 1 cut(s) 3206
BglII AGATCT 2 cut(s) 1902, 2710
BlnI CCTAGG 1 cut(s) 2734
BlpI GCTNAGC 1 cut(s) 3022
Bme18I GGWCC 9 cut(s) 719, 740, 760, 1755, 3134, 3175, 3222, 3572, 3609
BmeT110I CYCGRG 4 cut(s) 26, 503, 1038, 1634
BmgBI CACGTC 2 cut(s) 76, 1739
BmiI GGNNCC 9 cut(s) 37, 514, 1663, 1695, 2880, 2928, 3082, 3176, 3223
BmsI GCATC 8 cut(s) 115, 448, 454, 760, 1195, 1521, 2790, 3603
BoxI GACNNNNGTC 2 cut(s) 791, 1737
BpiI GAAGAC 3 cut(s) 2104, 2726, 3157
BplI GAGNNNNNCTC 8 cut(s) 1802, 1834, 2258, 2290, 3455, 3487, 3457, 3489
Bpu10I CCTNAGC 1 cut(s) 2804
Bpu1102I GCTNAGC 1 cut(s) 3022
BpuEI CTTGAG 2 cut(s) 531, 1331
BpuMI CCSGG 7 cut(s) 360, 504, 505, 869, 1635, 1636, 3522
Bsa29I ATCGAT 1 cut(s) 1788
BsaAI YACGTR 1 cut(s) 812
BsaBI GATNNNNATC 1 cut(s) 3307
BsaHI GRCGYC 6 cut(s) 497, 1662, 1866, 2289, 3195, 3297
BsaI GGTCTC 3 cut(s) 643, 1004, 2666
BsaWI WCCGGW 4 cut(s) 1941, 2278, 2504, 3177
BsaXI ACNNNNNCTCC 2 cut(s) 1529, 1559
Bse118I RCCGGY 3 cut(s) 1941, 3200, 3590
Bse1I ACTGG 5 cut(s) 900, 1153, 1168, 2779, 3343
Bse21I CCTNAGG 1 cut(s) 1870
Bse3DI GCAATG 3 cut(s) 1032, 1232, 2985
Bse8I GATNNNNATC 1 cut(s) 3307
BseAI TCCGGA 1 cut(s) 2504
BseBI CCWGG 4 cut(s) 524, 764, 1722, 2078
BseCI ATCGAT 1 cut(s) 1788
BseGI GGATG 9 cut(s) 355, 403, 727, 775, 967, 1629, 1686, 2359, 3594
BseJI GATNNNNATC 1 cut(s) 3307
BseMI GCAATG 3 cut(s) 1032, 1232, 2985
BseMII CTCAG 5 cut(s) 171, 1884, 2818, 3009, 3036
BseNI ACTGG 5 cut(s) 900, 1153, 1168, 2779, 3343
BsePI GCGCGC 1 cut(s) 560
BseSI GKGCMC 1 cut(s) 1625
BseX3I CGGCCG 1 cut(s) 536
BseXI GCAGC 6 cut(s) 514, 805, 1112, 1570, 2356, 3196
BseYI CCCAGC 2 cut(s) 619, 2119
Bsh1236I CGCG 5 cut(s) 562, 781, 797, 2678, 2951
Bsh1285I CGRYCG 1 cut(s) 539
BshFI GGCC 8 cut(s) 471, 538, 1628, 1688, 1717, 2056, 2118, 2621
BshNI GGYRCC 3 cut(s) 512, 1661, 3080
BshTI ACCGGT 1 cut(s) 1941
BshVI ATCGAT 1 cut(s) 1788
BsiEI CGRYCG 1 cut(s) 539
BsiHKAI GWGCWC 3 cut(s) 2004, 2754, 3237
BsiHKCI CYCGRG 4 cut(s) 26, 503, 1038, 1634
BslFI GGGAC 9 cut(s) 329, 380, 502, 1427, 1877, 3108, 3188, 3235, 3453
BsmAI GTCTC 9 cut(s) 504, 643, 1004, 1252, 1361, 1458, 2098, 2666, 3257
BsmBI CGTCTC 2 cut(s) 504, 1252
BsmFI GGGAC 9 cut(s) 329, 380, 502, 1427, 1877, 3108, 3188, 3235, 3453
BsmI GAATGC 1 cut(s) 2990
BsnI GGCC 8 cut(s) 471, 538, 1628, 1688, 1717, 2056, 2118, 2621
Bso31I GGTCTC 3 cut(s) 643, 1004, 2666
BsoBI CYCGRG 4 cut(s) 26, 503, 1038, 1634
Bsp1286I GDGCHC 6 cut(s) 862, 1625, 1641, 2004, 2754, 3237
Bsp13I TCCGGA 1 cut(s) 2504
Bsp1720I GCTNAGC 1 cut(s) 3022
Bsp19I CCATGG 2 cut(s) 1669, 3189
BspANI GGCC 8 cut(s) 471, 538, 1628, 1688, 1717, 2056, 2118, 2621
BspCNI CTCAG 5 cut(s) 172, 1883, 2817, 3008, 3035
BspDI ATCGAT 1 cut(s) 1788
BspEI TCCGGA 1 cut(s) 2504
BspFNI CGCG 5 cut(s) 562, 781, 797, 2678, 2951
BspHI TCATGA 2 cut(s) 202, 1987
BspLI GGNNCC 9 cut(s) 37, 514, 1663, 1695, 2880, 2928, 3082, 3176, 3223
BspMAI CTGCAG 2 cut(s) 3580, 3603
BspMI ACCTGC 1 cut(s) 3583
BspT107I GGYRCC 3 cut(s) 512, 1661, 3080
BspTI CTTAAG 1 cut(s) 3074
BspTNI GGTCTC 3 cut(s) 643, 1004, 2666
BsrBI CCGCTC 2 cut(s) 41, 533
BsrDI GCAATG 3 cut(s) 1032, 1232, 2985
BsrFI RCCGGY 3 cut(s) 1941, 3200, 3590
BsrI ACTGG 5 cut(s) 900, 1153, 1168, 2779, 3343
BssAI RCCGGY 3 cut(s) 1941, 3200, 3590
BssHII GCGCGC 1 cut(s) 560
BssNAI GTATAC 1 cut(s) 2520
BssNI GRCGYC 6 cut(s) 497, 1662, 1866, 2289, 3195, 3297
BssSI CACGAG 2 cut(s) 1767, 1808
BssT1I CCWWGG 7 cut(s) 703, 1669, 2248, 2734, 3041, 3170, 3189
Bst1107I GTATAC 1 cut(s) 2520
Bst2BI CACGAG 2 cut(s) 1767, 1808
Bst2UI CCWGG 4 cut(s) 524, 764, 1722, 2078
Bst4CI ACNGT 4 cut(s) 654, 998, 1344, 3099
Bst6I CTCTTC 1 cut(s) 2259
BstACI GRCGYC 6 cut(s) 497, 1662, 1866, 2289, 3195, 3297
BstAFI CTTAAG 1 cut(s) 3074
BstAPI GCANNNNNTGC 1 cut(s) 1346
BstBAI YACGTR 1 cut(s) 812
BstC8I GCNNGC 7 cut(s) 562, 625, 1098, 3202, 3233, 3237, 3592
BstDSI CCRYGG 6 cut(s) 1669, 1689, 1980, 2450, 2676, 3189
BstENI CCTNNNNNAGG 2 cut(s) 1616, 3321
BstF5I GGATG 9 cut(s) 355, 403, 727, 775, 967, 1629, 1686, 2359, 3594
BstFNI CGCG 5 cut(s) 562, 781, 797, 2678, 2951
BstH2I RGCGCY 3 cut(s) 1665, 3029, 3521
BstHHI GCGC 9 cut(s) 562, 564, 799, 1664, 2230, 2921, 2951, 3028, 3520
BstMAI GTCTC 9 cut(s) 504, 643, 1004, 1252, 1361, 1458, 2098, 2666, 3257
BstMCI CGRYCG 1 cut(s) 539
BstNI CCWGG 4 cut(s) 524, 764, 1722, 2078
BstNSI RCATGY 3 cut(s) 946, 1100, 2344
BstPAI GACNNNNGTC 2 cut(s) 791, 1737
BstSFI CTRYAG 3 cut(s) 1421, 3576, 3599
BstSLI GKGCMC 1 cut(s) 1625
BstUI CGCG 5 cut(s) 562, 781, 797, 2678, 2951
BstV1I GCAGC 6 cut(s) 514, 805, 1112, 1570, 2356, 3196
BstV2I GAAGAC 3 cut(s) 2104, 2726, 3157
BstX2I RGATCY 7 cut(s) 8, 307, 437, 1902, 2380, 2710, 2878
BstXI CCANNNNNNTGG 2 cut(s) 523, 3091
BstYI RGATCY 7 cut(s) 8, 307, 437, 1902, 2380, 2710, 2878
BstZ17I GTATAC 1 cut(s) 2520
BstZI CGGCCG 1 cut(s) 536
Bsu15I ATCGAT 1 cut(s) 1788
Bsu36I CCTNAGG 1 cut(s) 1870
BsuI GTATCC 1 cut(s) 710
BsuRI GGCC 8 cut(s) 471, 538, 1628, 1688, 1717, 2056, 2118, 2621
BsuTUI ATCGAT 1 cut(s) 1788
BtgI CCRYGG 6 cut(s) 1669, 1689, 1980, 2450, 2676, 3189
BtrI CACGTC 2 cut(s) 76, 1739
BtsCI GGATG 9 cut(s) 355, 403, 727, 775, 967, 1629, 1686, 2359, 3594
BtsI GCAGTG 3 cut(s) 1125, 2130, 3608
BtsIMutI CAGTG 5 cut(s) 1125, 1161, 2130, 3159, 3608
BveI ACCTGC 1 cut(s) 3583
Cac8I GCNNGC 7 cut(s) 562, 625, 1098, 3202, 3233, 3237, 3592
CciI TCATGA 2 cut(s) 202, 1987
CfoI GCGC 9 cut(s) 562, 564, 799, 1664, 2230, 2921, 2951, 3028, 3520
Cfr10I RCCGGY 3 cut(s) 1941, 3200, 3590
Cfr42I CCGCGG 1 cut(s) 2679
Cfr9I CCCGGG 2 cut(s) 503, 1634
ClaI ATCGAT 1 cut(s) 1788
CpoI CGGWCCG 1 cut(s) 1755
CseI GACGC 5 cut(s) 92, 1062, 1874, 3203, 3305
CsiI ACCWGGT 1 cut(s) 1720
Csp6I GTAC 7 cut(s) 974, 1150, 2201, 2652, 2830, 2959, 3081
CspAI ACCGGT 1 cut(s) 1941
CspI CGGWCCG 1 cut(s) 1755
CviQI GTAC 7 cut(s) 974, 1150, 2201, 2652, 2830, 2959, 3081
DinI GGCGCC 1 cut(s) 1663
DraIII CACNNNGTG 1 cut(s) 2456
DrdI GACNNNNNNGTC 3 cut(s) 219, 496, 2909
DseDI GACNNNNNNGTC 3 cut(s) 219, 496, 2909
EaeI YGGCCR 4 cut(s) 469, 536, 1626, 2619
EagI CGGCCG 1 cut(s) 536
Eam1104I CTCTTC 1 cut(s) 2259
EarI CTCTTC 1 cut(s) 2259
EciI GGCGGA 1 cut(s) 2124
EclXI CGGCCG 1 cut(s) 536
Eco130I CCWWGG 7 cut(s) 703, 1669, 2248, 2734, 3041, 3170, 3189
Eco24I GRGCYC 2 cut(s) 862, 1641
Eco31I GGTCTC 3 cut(s) 643, 1004, 2666
Eco32I GATATC 1 cut(s) 3372
Eco47I GGWCC 9 cut(s) 719, 740, 760, 1755, 3134, 3175, 3222, 3572, 3609
Eco52I CGGCCG 1 cut(s) 536
Eco57I CTGAAG 1 cut(s) 1496
Eco81I CCTNAGG 1 cut(s) 1870
Eco88I CYCGRG 4 cut(s) 26, 503, 1038, 1634
EcoNI CCTNNNNNAGG 2 cut(s) 1616, 3321
EcoO109I RGGNCCY 1 cut(s) 3572
EcoRI GAATTC 2 cut(s) 381, 2766
EcoRII CCWGG 4 cut(s) 522, 762, 1720, 2076
EcoRV GATATC 1 cut(s) 3372
EcoT14I CCWWGG 7 cut(s) 703, 1669, 2248, 2734, 3041, 3170, 3189
EcoT38I GRGCYC 2 cut(s) 862, 1641
EgeI GGCGCC 1 cut(s) 1663
EheI GGCGCC 1 cut(s) 1663
ErhI CCWWGG 7 cut(s) 703, 1669, 2248, 2734, 3041, 3170, 3189
Esp3I CGTCTC 2 cut(s) 504, 1252
FalI AAGNNNNNCTT 2 cut(s) 3047, 3079
FaqI GGGAC 9 cut(s) 329, 380, 502, 1427, 1877, 3108, 3188, 3235, 3453
FauI CCCGC 4 cut(s) 1386, 1475, 2225, 3595
FauNDI CATATG 1 cut(s) 2788
FbaI TGATCA 1 cut(s) 1782
FblI GTMKAC 3 cut(s) 1272, 2519, 3383
FokI GGATG 9 cut(s) 342, 390, 714, 782, 954, 1616, 1693, 2366, 3581
FriOI GRGCYC 2 cut(s) 862, 1641
GlaI GCGC 9 cut(s) 561, 563, 798, 1663, 2229, 2920, 2950, 3027, 3519
GsaI CCCAGC 2 cut(s) 623, 2123
HaeII RGCGCY 3 cut(s) 1665, 3029, 3521
HaeIII GGCC 8 cut(s) 471, 538, 1628, 1688, 1717, 2056, 2118, 2621
HgaI GACGC 5 cut(s) 92, 1062, 1874, 3203, 3305
HhaI GCGC 9 cut(s) 562, 564, 799, 1664, 2230, 2921, 2951, 3028, 3520
Hin1I GRCGYC 6 cut(s) 497, 1662, 1866, 2289, 3195, 3297
Hin6I GCGC 9 cut(s) 560, 562, 797, 1662, 2228, 2919, 2949, 3026, 3518
HinP1I GCGC 9 cut(s) 560, 562, 797, 1662, 2228, 2919, 2949, 3026, 3518
HincII GTYRAC 4 cut(s) 588, 1732, 1884, 3001
HindII GTYRAC 4 cut(s) 588, 1732, 1884, 3001
HindIII AAGCTT 1 cut(s) 332
HphI GGTGA 6 cut(s) 467, 719, 854, 1048, 1337, 3343
Hpy99I CGWCG 3 cut(s) 1661, 2906, 3299
HpyAV CCTTC 4 cut(s) 357, 418, 1812, 3563
HpyCH4III ACNGT 4 cut(s) 654, 998, 1344, 3099
Hsp92I GRCGYC 6 cut(s) 497, 1662, 1866, 2289, 3195, 3297
HspAI GCGC 9 cut(s) 560, 562, 797, 1662, 2228, 2919, 2949, 3026, 3518
KasI GGCGCC 1 cut(s) 1661
Kpn2I TCCGGA 1 cut(s) 2504
KpnI GGTACC 1 cut(s) 3084
KroI GCCGGC 2 cut(s) 3200, 3590
KroNI GCCGGC 2 cut(s) 3202, 3592
Ksp22I TGATCA 1 cut(s) 1782
KspI CCGCGG 1 cut(s) 2679
LmnI GCTCC 3 cut(s) 100, 1597, 1699
Lsp1109I GCAGC 6 cut(s) 514, 805, 1112, 1570, 2356, 3196
LweI GCATC 8 cut(s) 115, 448, 454, 760, 1195, 1521, 2790, 3603
MabI ACCWGGT 1 cut(s) 1720
MaeIII GTNAC 5 cut(s) 76, 1159, 1355, 2359, 2438
MbiI CCGCTC 2 cut(s) 41, 533
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 7 cut(s) 8, 307, 437, 1902, 2380, 2710, 2878
MhlI GDGCHC 6 cut(s) 862, 1625, 1641, 2004, 2754, 3237
MlsI TGGCCA 1 cut(s) 471
MluI ACGCGT 1 cut(s) 779
MluNI TGGCCA 1 cut(s) 471
Mly113I GGCGCC 1 cut(s) 1662
MmeI TCCRAC 4 cut(s) 621, 764, 1316, 2134
Mox20I TGGCCA 1 cut(s) 471
MroI TCCGGA 1 cut(s) 2504
MroNI GCCGGC 2 cut(s) 3200, 3590
MroXI GAANNNNTTC 1 cut(s) 3447
MscI TGGCCA 1 cut(s) 471
MseI TTAA 8 cut(s) 126, 849, 882, 1017, 1319, 2495, 2814, 3075
MslI CAYNNNNRTG 5 cut(s) 450, 453, 1362, 2354, 2846
Msp20I TGGCCA 1 cut(s) 471
MspA1I CMGCKG 2 cut(s) 1583, 2678
MspCI CTTAAG 1 cut(s) 3074
MunI CAATTG 1 cut(s) 552
Mva1269I GAATGC 1 cut(s) 2990
MvaI CCWGG 4 cut(s) 524, 764, 1722, 2078
MvnI CGCG 5 cut(s) 562, 781, 797, 2678, 2951
NaeI GCCGGC 2 cut(s) 3202, 3592
NarI GGCGCC 1 cut(s) 1662
NciI CCSGG 7 cut(s) 360, 504, 505, 869, 1635, 1636, 3522
NcoI CCATGG 2 cut(s) 1669, 3189
NdeI CATATG 1 cut(s) 2788
NgoMIV GCCGGC 2 cut(s) 3200, 3590
NlaIV GGNNCC 9 cut(s) 37, 514, 1663, 1695, 2880, 2928, 3082, 3176, 3223
NmeAIII GCCGAG 3 cut(s) 514, 2163, 3286
NmuCI GTSAC 4 cut(s) 76, 1159, 1355, 2438
NspI RCATGY 3 cut(s) 946, 1100, 2344
PaeI GCATGC 1 cut(s) 1100
PaeR7I CTCGAG 1 cut(s) 1038
PagI TCATGA 2 cut(s) 202, 1987
PalAI GGCGCGCC 1 cut(s) 560
PauI GCGCGC 1 cut(s) 560
PcsI WCGNNNNNNNCGW 2 cut(s) 1002, 2010
PctI GAATGC 1 cut(s) 2990
PdiI GCCGGC 2 cut(s) 3202, 3592
PdmI GAANNNNTTC 1 cut(s) 3447
PfeI GAWTC 6 cut(s) 271, 284, 1286, 1798, 1853, 2387
PflFI GACNNNGTC 1 cut(s) 351
PfoI TCCNGGA 1 cut(s) 358
PinAI ACCGGT 1 cut(s) 1941
PluTI GGCGCC 1 cut(s) 1665
Ppu21I YACGTR 1 cut(s) 812
PpuMI RGGWCCY 1 cut(s) 3572
PshAI GACNNNNGTC 2 cut(s) 791, 1737
Psp5II RGGWCCY 1 cut(s) 3572
Psp6I CCWGG 4 cut(s) 522, 762, 1720, 2076
PspFI CCCAGC 2 cut(s) 619, 2119
PspGI CCWGG 4 cut(s) 522, 762, 1720, 2076
PspN4I GGNNCC 9 cut(s) 37, 514, 1663, 1695, 2880, 2928, 3082, 3176, 3223
PspPPI RGGWCCY 1 cut(s) 3572
PspXI VCTCGAGB 1 cut(s) 1038
PsrI GAACNNNNNNTAC 4 cut(s) 957, 966, 989, 998
PstI CTGCAG 2 cut(s) 3580, 3603
PsuI RGATCY 7 cut(s) 8, 307, 437, 1902, 2380, 2710, 2878
PsyI GACNNNGTC 1 cut(s) 351
PteI GCGCGC 1 cut(s) 560
PvuII CAGCTG 1 cut(s) 1583
RsaI GTAC 7 cut(s) 975, 1151, 2202, 2653, 2831, 2960, 3082
RsaNI GTAC 7 cut(s) 974, 1150, 2201, 2652, 2830, 2959, 3081
RseI CAYNNNNRTG 5 cut(s) 450, 453, 1362, 2354, 2846
Rsr2I CGGWCCG 1 cut(s) 1755
RsrII CGGWCCG 1 cut(s) 1755
SacII CCGCGG 1 cut(s) 2679
SaqAI TTAA 8 cut(s) 126, 849, 882, 1017, 1319, 2495, 2814, 3075
SduI GDGCHC 6 cut(s) 862, 1625, 1641, 2004, 2754, 3237
SexAI ACCWGGT 1 cut(s) 1720
SfaNI GCATC 8 cut(s) 115, 448, 454, 760, 1195, 1521, 2790, 3603
SfcI CTRYAG 3 cut(s) 1421, 3576, 3599
SfoI GGCGCC 1 cut(s) 1663
Sfr274I CTCGAG 1 cut(s) 1038
Sfr303I CCGCGG 1 cut(s) 2679
SgrBI CCGCGG 1 cut(s) 2679
SgsI GGCGCGCC 1 cut(s) 560
SinI GGWCC 9 cut(s) 719, 740, 760, 1755, 3134, 3175, 3222, 3572, 3609
SlaI CTCGAG 1 cut(s) 1038
SmaI CCCGGG 2 cut(s) 505, 1636
SmiMI CAYNNNNRTG 5 cut(s) 450, 453, 1362, 2354, 2846
SmlI CTYRAG 4 cut(s) 546, 1038, 1310, 3074
SmoI CTYRAG 4 cut(s) 546, 1038, 1310, 3074
SphI GCATGC 1 cut(s) 1100
SspDI GGCGCC 1 cut(s) 1661
SspI AATATT 2 cut(s) 431, 1092
StyI CCWWGG 7 cut(s) 703, 1669, 2248, 2734, 3041, 3170, 3189
TaaI ACNGT 4 cut(s) 654, 998, 1344, 3099
TaqII GACCGA 1 cut(s) 1772
TatI WGTACW 1 cut(s) 2958
TauI GCSGC 8 cut(s) 533, 1024, 1652, 1655, 1667, 2681, 3200, 3248
TfiI GAWTC 6 cut(s) 271, 284, 1286, 1798, 1853, 2387
Tru1I TTAA 8 cut(s) 126, 849, 882, 1017, 1319, 2495, 2814, 3075
Tru9I TTAA 8 cut(s) 126, 849, 882, 1017, 1319, 2495, 2814, 3075
TscAI CASTG 5 cut(s) 1132, 1168, 2130, 3166, 3608
TseFI GTSAC 4 cut(s) 76, 1159, 1355, 2438
TseI GCWGC 6 cut(s) 527, 818, 1100, 1583, 2344, 3209
Tsp45I GTSAC 4 cut(s) 76, 1159, 1355, 2438
TspGWI ACGGA 7 cut(s) 95, 225, 1768, 1969, 2467, 2505, 2876
TspMI CCCGGG 2 cut(s) 503, 1634
TspRI CASTG 5 cut(s) 1132, 1168, 2130, 3166, 3608
Tth111I GACNNNGTC 1 cut(s) 351
Vha464I CTTAAG 1 cut(s) 3074
VpaK11BI GGWCC 9 cut(s) 719, 740, 760, 1755, 3134, 3175, 3222, 3572, 3609
XagI CCTNNNNNAGG 2 cut(s) 1616, 3321
XapI RAATTY 3 cut(s) 381, 911, 2766
XbaI TCTAGA 1 cut(s) 2383
XceI RCATGY 3 cut(s) 946, 1100, 2344
XcmI CCANNNNNNNNNTGG 2 cut(s) 734, 1160
XhoI CTCGAG 1 cut(s) 1038
XmaI CCCGGG 2 cut(s) 503, 1634
XmaJI CCTAGG 1 cut(s) 2734
XmiI GTMKAC 3 cut(s) 1272, 2519, 3383
XmnI GAANNNNTTC 1 cut(s) 3447
ZraI GACGTC 2 cut(s) 498, 2290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.