aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 535 |
| AccB1I | GGYRCC | 1 cut(s) | 87 |
| AciI | CCGC | 1 cut(s) | 79 |
| AdeI | CACNNNGTG | 1 cut(s) | 212 |
| AfaI | GTAC | 1 cut(s) | 608 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 169, 170, 171 |
| AgsI | TTSAA | 1 cut(s) | 17 |
| AluBI | AGCT | 1 cut(s) | 134 |
| AluI | AGCT | 1 cut(s) | 134 |
| Alw21I | GWGCWC | 1 cut(s) | 441 |
| AoxI | GGCC | 2 cut(s) | 256, 491 |
| ApeKI | GCWGC | 1 cut(s) | 134 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 510 |
| AspA2I | CCTAGG | 1 cut(s) | 82 |
| AspS9I | GGNCC | 1 cut(s) | 554 |
| AsuC2I | CCSGG | 1 cut(s) | 93 |
| AsuHPI | GGTGA | 2 cut(s) | 22, 271 |
| AvaII | GGWCC | 1 cut(s) | 554 |
| AvrII | CCTAGG | 1 cut(s) | 82 |
| BaeGI | GKGCMC | 2 cut(s) | 90, 177 |
| BanI | GGYRCC | 1 cut(s) | 87 |
| Bbv12I | GWGCWC | 1 cut(s) | 441 |
| BbvI | GCAGC | 1 cut(s) | 121 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 77, 111 |
| BclI | TGATCA | 1 cut(s) | 9 |
| BcnI | CCSGG | 1 cut(s) | 93 |
| BfaI | CTAG | 1 cut(s) | 83 |
| BfuAI | ACCTGC | 1 cut(s) | 535 |
| BisI | GCNGC | 1 cut(s) | 135 |
| BlnI | CCTAGG | 1 cut(s) | 82 |
| BlsI | GCNGC | 1 cut(s) | 136 |
| Bme1390I | CCNGG | 1 cut(s) | 93 |
| Bme18I | GGWCC | 1 cut(s) | 554 |
| BmgT120I | GGNCC | 1 cut(s) | 554 |
| BmiI | GGNNCC | 2 cut(s) | 89, 237 |
| BmrFI | CCNGG | 1 cut(s) | 93 |
| BpuEI | CTTGAG | 2 cut(s) | 408, 578 |
| BpuMI | CCSGG | 1 cut(s) | 93 |
| BsaBI | GATNNNNATC | 1 cut(s) | 8 |
| BsaJI | CCNNGG | 4 cut(s) | 82, 91, 241, 283 |
| BsaWI | WCCGGW | 1 cut(s) | 556 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 207, 237 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 169, 170, 171 |
| Bse8I | GATNNNNATC | 1 cut(s) | 8 |
| BseDI | CCNNGG | 4 cut(s) | 82, 91, 241, 283 |
| BseJI | GATNNNNATC | 1 cut(s) | 8 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 169, 170, 171 |
| BseSI | GKGCMC | 2 cut(s) | 90, 177 |
| BseXI | GCAGC | 1 cut(s) | 121 |
| BsgI | GTGCAG | 1 cut(s) | 189 |
| Bsh1285I | CGRYCG | 1 cut(s) | 97 |
| BshFI | GGCC | 2 cut(s) | 258, 493 |
| BshNI | GGYRCC | 1 cut(s) | 87 |
| BsiEI | CGRYCG | 1 cut(s) | 97 |
| BsiHKAI | GWGCWC | 1 cut(s) | 441 |
| BsiSI | CCGG | 2 cut(s) | 93, 557 |
| BslFI | GGGAC | 2 cut(s) | 62, 146 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 169, 170, 171 |
| BsmFI | GGGAC | 2 cut(s) | 62, 146 |
| BsnI | GGCC | 2 cut(s) | 258, 493 |
| Bsp1286I | GDGCHC | 3 cut(s) | 90, 177, 441 |
| Bsp143I | GATC | 3 cut(s) | 9, 108, 119 |
| Bsp19I | CCATGG | 1 cut(s) | 283 |
| BspACI | CCGC | 1 cut(s) | 79 |
| BspANI | GGCC | 2 cut(s) | 258, 493 |
| BspLI | GGNNCC | 2 cut(s) | 89, 237 |
| BspMI | ACCTGC | 1 cut(s) | 535 |
| BspT107I | GGYRCC | 1 cut(s) | 87 |
| BssECI | CCNNGG | 4 cut(s) | 82, 91, 241, 283 |
| BssMI | GATC | 3 cut(s) | 9, 108, 119 |
| BssT1I | CCWWGG | 3 cut(s) | 82, 241, 283 |
| Bst4CI | ACNGT | 4 cut(s) | 28, 38, 179, 418 |
| Bst6I | CTCTTC | 2 cut(s) | 241, 286 |
| BstC8I | GCNNGC | 1 cut(s) | 147 |
| BstDEI | CTNAG | 2 cut(s) | 209, 343 |
| BstDSI | CCRYGG | 1 cut(s) | 283 |
| BstKTI | GATC | 3 cut(s) | 12, 111, 122 |
| BstMBI | GATC | 3 cut(s) | 9, 108, 119 |
| BstMCI | CGRYCG | 1 cut(s) | 97 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 515 |
| BstSCI | CCNGG | 1 cut(s) | 91 |
| BstSLI | GKGCMC | 2 cut(s) | 90, 177 |
| BstV1I | GCAGC | 1 cut(s) | 121 |
| BsuRI | GGCC | 2 cut(s) | 258, 493 |
| BtgI | CCRYGG | 1 cut(s) | 283 |
| BtsI | GCAGTG | 1 cut(s) | 195 |
| BtsIMutI | CAGTG | 4 cut(s) | 43, 51, 195, 414 |
| BveI | ACCTGC | 1 cut(s) | 535 |
| Cac8I | GCNNGC | 1 cut(s) | 147 |
| Cfr13I | GGNCC | 1 cut(s) | 554 |
| CseI | GACGC | 1 cut(s) | 311 |
| Csp6I | GTAC | 1 cut(s) | 607 |
| CviAII | CATG | 3 cut(s) | 74, 103, 284 |
| CviJI | RGCY | 7 cut(s) | 67, 134, 230, 236, 258, 493, 587 |
| CviKI_1 | RGCY | 7 cut(s) | 67, 134, 230, 236, 258, 493, 587 |
| CviQI | GTAC | 1 cut(s) | 607 |
| DdeI | CTNAG | 2 cut(s) | 209, 343 |
| DpnI | GATC | 3 cut(s) | 11, 110, 121 |
| DpnII | GATC | 3 cut(s) | 9, 108, 119 |
| DraI | TTTAAA | 1 cut(s) | 316 |
| DraIII | CACNNNGTG | 1 cut(s) | 212 |
| Eam1104I | CTCTTC | 2 cut(s) | 241, 286 |
| EarI | CTCTTC | 2 cut(s) | 241, 286 |
| Eco130I | CCWWGG | 3 cut(s) | 82, 241, 283 |
| Eco32I | GATATC | 1 cut(s) | 448 |
| Eco47I | GGWCC | 1 cut(s) | 554 |
| EcoRV | GATATC | 1 cut(s) | 448 |
| EcoT14I | CCWWGG | 3 cut(s) | 82, 241, 283 |
| ErhI | CCWWGG | 3 cut(s) | 82, 241, 283 |
| FaeI | CATG | 3 cut(s) | 77, 106, 287 |
| FaqI | GGGAC | 2 cut(s) | 62, 146 |
| FatI | CATG | 3 cut(s) | 73, 102, 283 |
| FbaI | TGATCA | 1 cut(s) | 9 |
| Fnu4HI | GCNGC | 1 cut(s) | 135 |
| Fsp4HI | GCNGC | 1 cut(s) | 135 |
| FspBI | CTAG | 1 cut(s) | 83 |
| GluI | GCNGC | 1 cut(s) | 135 |
| HaeIII | GGCC | 2 cut(s) | 258, 493 |
| HapII | CCGG | 2 cut(s) | 93, 557 |
| HgaI | GACGC | 1 cut(s) | 311 |
| Hin1II | CATG | 3 cut(s) | 77, 106, 287 |
| HincII | GTYRAC | 1 cut(s) | 476 |
| HindII | GTYRAC | 1 cut(s) | 476 |
| HinfI | GANTC | 5 cut(s) | 4, 17, 56, 322, 527 |
| HpaII | CCGG | 2 cut(s) | 93, 557 |
| HphI | GGTGA | 2 cut(s) | 22, 271 |
| Hpy166II | GTNNAC | 1 cut(s) | 476 |
| Hpy188I | TCNGA | 3 cut(s) | 9, 309, 534 |
| Hpy188III | TCNNGA | 4 cut(s) | 98, 112, 326, 425 |
| Hpy8I | GTNNAC | 1 cut(s) | 476 |
| HpyCH4III | ACNGT | 4 cut(s) | 28, 38, 179, 418 |
| HpyCH4V | TGCA | 6 cut(s) | 137, 149, 200, 206, 410, 600 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 515 |
| HpyF3I | CTNAG | 2 cut(s) | 209, 343 |
| Hsp92II | CATG | 3 cut(s) | 77, 106, 287 |
| Ksp22I | TGATCA | 1 cut(s) | 9 |
| Kzo9I | GATC | 3 cut(s) | 9, 108, 119 |
| LmnI | GCTCC | 2 cut(s) | 241, 310 |
| LpnPI | CCDG | 8 cut(s) | 49, 106, 144, 159, 339, 530, 570, 585 |
| Lsp1109I | GCAGC | 1 cut(s) | 121 |
| MaeI | CTAG | 1 cut(s) | 83 |
| MaeIII | GTNAC | 3 cut(s) | 28, 98, 112 |
| MalI | GATC | 3 cut(s) | 11, 110, 121 |
| MboI | GATC | 3 cut(s) | 9, 108, 119 |
| MboII | GAAGA | 3 cut(s) | 258, 300, 303 |
| MhlI | GDGCHC | 3 cut(s) | 90, 177, 441 |
| MluCI | AATT | 3 cut(s) | 349, 510, 521 |
| MlyI | GAGTC | 1 cut(s) | 50 |
| MnlI | CCTC | 9 cut(s) | 185, 209, 220, 242, 248, 287, 534, 597, 602 |
| MroXI | GAANNNNTTC | 1 cut(s) | 510 |
| MseI | TTAA | 2 cut(s) | 315, 582 |
| MslI | CAYNNNNRTG | 1 cut(s) | 396 |
| MspA1I | CMGCKG | 1 cut(s) | 134 |
| MspI | CCGG | 2 cut(s) | 93, 557 |
| MspR9I | CCNGG | 1 cut(s) | 93 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 515 |
| NciI | CCSGG | 1 cut(s) | 93 |
| NcoI | CCATGG | 1 cut(s) | 283 |
| NdeII | GATC | 3 cut(s) | 9, 108, 119 |
| NlaIII | CATG | 3 cut(s) | 77, 106, 287 |
| NlaIV | GGNNCC | 2 cut(s) | 89, 237 |
| NmuCI | GTSAC | 3 cut(s) | 28, 98, 112 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 447 |
| PdmI | GAANNNNTTC | 1 cut(s) | 510 |
| PfeI | GAWTC | 4 cut(s) | 4, 17, 322, 527 |
| PkrI | GCNGC | 1 cut(s) | 136 |
| PleI | GAGTC | 1 cut(s) | 50 |
| PpsI | GAGTC | 1 cut(s) | 50 |
| PspN4I | GGNNCC | 2 cut(s) | 89, 237 |
| PspPI | GGNCC | 1 cut(s) | 554 |
| PvuII | CAGCTG | 1 cut(s) | 134 |
| RsaI | GTAC | 1 cut(s) | 608 |
| RsaNI | GTAC | 1 cut(s) | 607 |
| RseI | CAYNNNNRTG | 1 cut(s) | 396 |
| SaqAI | TTAA | 2 cut(s) | 315, 582 |
| SatI | GCNGC | 1 cut(s) | 135 |
| Sau3AI | GATC | 3 cut(s) | 9, 108, 119 |
| Sau96I | GGNCC | 1 cut(s) | 554 |
| SchI | GAGTC | 1 cut(s) | 50 |
| ScrFI | CCNGG | 1 cut(s) | 93 |
| SduI | GDGCHC | 3 cut(s) | 90, 177, 441 |
| SetI | ASST | 5 cut(s) | 136, 155, 220, 549, 608 |
| SinI | GGWCC | 1 cut(s) | 554 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 396 |
| SmlI | CTYRAG | 2 cut(s) | 423, 593 |
| SmoI | CTYRAG | 2 cut(s) | 423, 593 |
| Sse9I | AATT | 3 cut(s) | 349, 510, 521 |
| SsiI | CCGC | 1 cut(s) | 79 |
| SspMI | CTAG | 1 cut(s) | 83 |
| StyD4I | CCNGG | 1 cut(s) | 91 |
| StyI | CCWWGG | 3 cut(s) | 82, 241, 283 |
| TaaI | ACNGT | 4 cut(s) | 28, 38, 179, 418 |
| TaqI | TCGA | 2 cut(s) | 268, 450 |
| TasI | AATT | 3 cut(s) | 349, 510, 521 |
| TfiI | GAWTC | 4 cut(s) | 4, 17, 322, 527 |
| Tru1I | TTAA | 2 cut(s) | 315, 582 |
| Tru9I | TTAA | 2 cut(s) | 315, 582 |
| TscAI | CASTG | 4 cut(s) | 43, 58, 202, 421 |
| TseFI | GTSAC | 3 cut(s) | 28, 98, 112 |
| TseI | GCWGC | 1 cut(s) | 134 |
| Tsp45I | GTSAC | 3 cut(s) | 28, 98, 112 |
| TspDTI | ATGAA | 1 cut(s) | 376 |
| TspRI | CASTG | 4 cut(s) | 43, 58, 202, 421 |
| VpaK11BI | GGWCC | 1 cut(s) | 554 |
| XmaJI | CCTAGG | 1 cut(s) | 82 |
| XmnI | GAANNNNTTC | 1 cut(s) | 510 |
| XspI | CTAG | 1 cut(s) | 83 |