aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 312 |
| Acc16I | TGCGCA | 1 cut(s) | 197 |
| Acc36I | ACCTGC | 1 cut(s) | 289 |
| AciI | CCGC | 2 cut(s) | 174, 356 |
| AclI | AACGTT | 1 cut(s) | 102 |
| AclWI | GGATC | 1 cut(s) | 158 |
| AcsI | RAATTY | 1 cut(s) | 201 |
| AcyI | GRCGYC | 1 cut(s) | 309 |
| AdeI | CACNNNGTG | 1 cut(s) | 182 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 86, 89 |
| AjiI | CACGTC | 2 cut(s) | 125, 135 |
| AluBI | AGCT | 2 cut(s) | 16, 257 |
| AluI | AGCT | 2 cut(s) | 16, 257 |
| Alw21I | GWGCWC | 2 cut(s) | 8, 18 |
| Alw26I | GTCTC | 2 cut(s) | 62, 159 |
| AlwI | GGATC | 1 cut(s) | 158 |
| ApeKI | GCWGC | 1 cut(s) | 284 |
| ApoI | RAATTY | 1 cut(s) | 201 |
| AspLEI | GCGC | 1 cut(s) | 198 |
| BanII | GRGCYC | 1 cut(s) | 18 |
| BbsI | GAAGAC | 1 cut(s) | 67 |
| Bbv12I | GWGCWC | 2 cut(s) | 8, 18 |
| BbvI | GCAGC | 1 cut(s) | 271 |
| BccI | CCATC | 2 cut(s) | 329, 346 |
| BcoDI | GTCTC | 2 cut(s) | 62, 159 |
| BfaI | CTAG | 3 cut(s) | 42, 206, 254 |
| BfmI | CTRYAG | 2 cut(s) | 212, 315 |
| BfuAI | ACCTGC | 1 cut(s) | 289 |
| BglII | AGATCT | 1 cut(s) | 28 |
| BisI | GCNGC | 2 cut(s) | 285, 357 |
| BlsI | GCNGC | 2 cut(s) | 286, 358 |
| BmgBI | CACGTC | 2 cut(s) | 125, 135 |
| BpiI | GAAGAC | 1 cut(s) | 67 |
| BsaHI | GRCGYC | 1 cut(s) | 309 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 86, 89 |
| Bse1I | ACTGG | 1 cut(s) | 364 |
| BseGI | GGATG | 2 cut(s) | 326, 327 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 86, 89 |
| BseNI | ACTGG | 1 cut(s) | 364 |
| BseXI | GCAGC | 1 cut(s) | 271 |
| BsiHKAI | GWGCWC | 2 cut(s) | 8, 18 |
| BslFI | GGGAC | 3 cut(s) | 128, 296, 359 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 86, 89 |
| BsmAI | GTCTC | 2 cut(s) | 62, 159 |
| BsmFI | GGGAC | 3 cut(s) | 128, 296, 359 |
| Bsp1286I | GDGCHC | 2 cut(s) | 8, 18 |
| Bsp143I | GATC | 4 cut(s) | 28, 163, 250, 269 |
| BspACI | CCGC | 2 cut(s) | 174, 356 |
| BspMI | ACCTGC | 1 cut(s) | 289 |
| BspPI | GGATC | 1 cut(s) | 158 |
| BsrI | ACTGG | 1 cut(s) | 364 |
| BssMI | GATC | 4 cut(s) | 28, 163, 250, 269 |
| BssNI | GRCGYC | 1 cut(s) | 309 |
| Bst4CI | ACNGT | 2 cut(s) | 73, 84 |
| BstACI | GRCGYC | 1 cut(s) | 309 |
| BstC8I | GCNNGC | 1 cut(s) | 297 |
| BstF5I | GGATG | 2 cut(s) | 326, 327 |
| BstHHI | GCGC | 1 cut(s) | 198 |
| BstKTI | GATC | 4 cut(s) | 31, 166, 253, 272 |
| BstMAI | GTCTC | 2 cut(s) | 62, 159 |
| BstMBI | GATC | 4 cut(s) | 28, 163, 250, 269 |
| BstSFI | CTRYAG | 2 cut(s) | 212, 315 |
| BstV1I | GCAGC | 1 cut(s) | 271 |
| BstV2I | GAAGAC | 1 cut(s) | 67 |
| BstX2I | RGATCY | 1 cut(s) | 28 |
| BstYI | RGATCY | 1 cut(s) | 28 |
| BtrI | CACGTC | 2 cut(s) | 125, 135 |
| BtsCI | GGATG | 2 cut(s) | 326, 327 |
| BveI | ACCTGC | 1 cut(s) | 289 |
| Cac8I | GCNNGC | 1 cut(s) | 297 |
| CfoI | GCGC | 1 cut(s) | 198 |
| CseI | GACGC | 1 cut(s) | 248 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 28, 63 |
| CviJI | RGCY | 4 cut(s) | 16, 224, 257, 359 |
| CviKI_1 | RGCY | 4 cut(s) | 16, 224, 257, 359 |
| DpnI | GATC | 4 cut(s) | 30, 165, 252, 271 |
| DpnII | GATC | 4 cut(s) | 28, 163, 250, 269 |
| DraIII | CACNNNGTG | 1 cut(s) | 182 |
| Ecl136II | GAGCTC | 1 cut(s) | 16 |
| Eco24I | GRGCYC | 1 cut(s) | 18 |
| Eco53kI | GAGCTC | 1 cut(s) | 16 |
| EcoICRI | GAGCTC | 1 cut(s) | 16 |
| EcoT38I | GRGCYC | 1 cut(s) | 18 |
| FaiI | YATR | 8 cut(s) | 152, 233, 274, 276, 278, 317, 370, 372 |
| FaqI | GGGAC | 3 cut(s) | 128, 296, 359 |
| FauNDI | CATATG | 1 cut(s) | 370 |
| Fnu4HI | GCNGC | 2 cut(s) | 285, 357 |
| FokI | GGATG | 2 cut(s) | 314, 333 |
| FriOI | GRGCYC | 1 cut(s) | 18 |
| Fsp4HI | GCNGC | 2 cut(s) | 285, 357 |
| FspBI | CTAG | 3 cut(s) | 42, 206, 254 |
| FspI | TGCGCA | 1 cut(s) | 197 |
| GlaI | GCGC | 1 cut(s) | 197 |
| GluI | GCNGC | 2 cut(s) | 285, 357 |
| HgaI | GACGC | 1 cut(s) | 248 |
| HhaI | GCGC | 1 cut(s) | 198 |
| Hin1I | GRCGYC | 1 cut(s) | 309 |
| Hin6I | GCGC | 1 cut(s) | 196 |
| HinP1I | GCGC | 1 cut(s) | 196 |
| Hpy188I | TCNGA | 1 cut(s) | 250 |
| Hpy188III | TCNNGA | 4 cut(s) | 19, 113, 161, 206 |
| Hpy99I | CGWCG | 2 cut(s) | 126, 352 |
| HpyCH4III | ACNGT | 2 cut(s) | 73, 84 |
| HpyCH4IV | ACGT | 4 cut(s) | 102, 124, 134, 309 |
| HpyCH4V | TGCA | 2 cut(s) | 287, 299 |
| HpySE526I | ACGT | 4 cut(s) | 102, 124, 134, 309 |
| Hsp92I | GRCGYC | 1 cut(s) | 309 |
| HspAI | GCGC | 1 cut(s) | 196 |
| Kzo9I | GATC | 4 cut(s) | 28, 163, 250, 269 |
| LpnPI | CCDG | 7 cut(s) | 9, 126, 249, 294, 309, 345, 377 |
| Lsp1109I | GCAGC | 1 cut(s) | 271 |
| MaeI | CTAG | 3 cut(s) | 42, 206, 254 |
| MaeII | ACGT | 4 cut(s) | 102, 124, 134, 309 |
| MaeIII | GTNAC | 2 cut(s) | 56, 262 |
| MalI | GATC | 4 cut(s) | 30, 165, 252, 271 |
| MboI | GATC | 4 cut(s) | 28, 163, 250, 269 |
| MboII | GAAGA | 1 cut(s) | 67 |
| MflI | RGATCY | 1 cut(s) | 28 |
| MhlI | GDGCHC | 2 cut(s) | 8, 18 |
| MluCI | AATT | 3 cut(s) | 52, 128, 201 |
| MnlI | CCTC | 1 cut(s) | 335 |
| MslI | CAYNNNNRTG | 1 cut(s) | 63 |
| NdeI | CATATG | 1 cut(s) | 370 |
| NdeII | GATC | 4 cut(s) | 28, 163, 250, 269 |
| NmuCI | GTSAC | 2 cut(s) | 56, 262 |
| NsbI | TGCGCA | 1 cut(s) | 197 |
| PkrI | GCNGC | 2 cut(s) | 286, 358 |
| Psp124BI | GAGCTC | 1 cut(s) | 18 |
| Psp1406I | AACGTT | 1 cut(s) | 102 |
| PsuI | RGATCY | 1 cut(s) | 28 |
| RseI | CAYNNNNRTG | 1 cut(s) | 63 |
| SacI | GAGCTC | 1 cut(s) | 18 |
| SatI | GCNGC | 2 cut(s) | 285, 357 |
| Sau3AI | GATC | 4 cut(s) | 28, 163, 250, 269 |
| SduI | GDGCHC | 2 cut(s) | 8, 18 |
| SetI | ASST | 8 cut(s) | 18, 105, 127, 137, 213, 259, 283, 312 |
| SfcI | CTRYAG | 2 cut(s) | 212, 315 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 63 |
| Sse9I | AATT | 3 cut(s) | 52, 128, 201 |
| SsiI | CCGC | 2 cut(s) | 174, 356 |
| SspMI | CTAG | 3 cut(s) | 42, 206, 254 |
| SstI | GAGCTC | 1 cut(s) | 18 |
| TaaI | ACNGT | 2 cut(s) | 73, 84 |
| TaiI | ACGT | 4 cut(s) | 105, 127, 137, 312 |
| TaqI | TCGA | 1 cut(s) | 12 |
| TasI | AATT | 3 cut(s) | 52, 128, 201 |
| TauI | GCSGC | 1 cut(s) | 359 |
| TseFI | GTSAC | 2 cut(s) | 56, 262 |
| TseI | GCWGC | 1 cut(s) | 284 |
| Tsp45I | GTSAC | 2 cut(s) | 56, 262 |
| TspDTI | ATGAA | 2 cut(s) | 132, 204 |
| XapI | RAATTY | 1 cut(s) | 201 |
| XbaI | TCTAGA | 1 cut(s) | 205 |
| XspI | CTAG | 3 cut(s) | 42, 206, 254 |
| ZraI | GACGTC | 1 cut(s) | 310 |