aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 307 |
| AccI | GTMKAC | 2 cut(s) | 223, 270 |
| AciI | CCGC | 4 cut(s) | 14, 108, 130, 261 |
| AclI | AACGTT | 1 cut(s) | 36 |
| AclWI | GGATC | 1 cut(s) | 92 |
| AcsI | RAATTY | 1 cut(s) | 135 |
| AcyI | GRCGYC | 1 cut(s) | 304 |
| AdeI | CACNNNGTG | 1 cut(s) | 116 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 20, 23, 280 |
| AjiI | CACGTC | 2 cut(s) | 59, 69 |
| AluBI | AGCT | 1 cut(s) | 191 |
| AluI | AGCT | 1 cut(s) | 191 |
| Alw26I | GTCTC | 1 cut(s) | 93 |
| AlwI | GGATC | 1 cut(s) | 92 |
| ApeKI | GCWGC | 1 cut(s) | 328 |
| ApoI | RAATTY | 1 cut(s) | 135 |
| BccI | CCATC | 1 cut(s) | 285 |
| BcoDI | GTCTC | 1 cut(s) | 93 |
| BfaI | CTAG | 2 cut(s) | 140, 188 |
| BfmI | CTRYAG | 1 cut(s) | 146 |
| BglII | AGATCT | 1 cut(s) | 142 |
| BisI | GCNGC | 2 cut(s) | 261, 329 |
| BlsI | GCNGC | 2 cut(s) | 262, 330 |
| BmgBI | CACGTC | 2 cut(s) | 59, 69 |
| BsaHI | GRCGYC | 1 cut(s) | 304 |
| BsaJI | CCNNGG | 1 cut(s) | 264 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 20, 23, 280 |
| Bse1I | ACTGG | 1 cut(s) | 257 |
| BseDI | CCNNGG | 1 cut(s) | 264 |
| BseGI | GGATG | 1 cut(s) | 291 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 20, 23, 280 |
| BseNI | ACTGG | 1 cut(s) | 257 |
| BslFI | GGGAC | 3 cut(s) | 62, 256, 297 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 20, 23, 280 |
| BsmAI | GTCTC | 1 cut(s) | 93 |
| BsmFI | GGGAC | 3 cut(s) | 62, 256, 297 |
| Bsp143I | GATC | 4 cut(s) | 97, 142, 184, 203 |
| Bsp19I | CCATGG | 1 cut(s) | 264 |
| BspACI | CCGC | 4 cut(s) | 14, 108, 130, 261 |
| BspPI | GGATC | 1 cut(s) | 92 |
| BsrI | ACTGG | 1 cut(s) | 257 |
| BssECI | CCNNGG | 1 cut(s) | 264 |
| BssMI | GATC | 4 cut(s) | 97, 142, 184, 203 |
| BssNI | GRCGYC | 1 cut(s) | 304 |
| BssT1I | CCWWGG | 1 cut(s) | 264 |
| Bst4CI | ACNGT | 1 cut(s) | 43 |
| BstACI | GRCGYC | 1 cut(s) | 304 |
| BstC8I | GCNNGC | 1 cut(s) | 318 |
| BstDSI | CCRYGG | 1 cut(s) | 264 |
| BstF5I | GGATG | 1 cut(s) | 291 |
| BstKTI | GATC | 4 cut(s) | 100, 145, 187, 206 |
| BstMAI | GTCTC | 1 cut(s) | 93 |
| BstMBI | GATC | 4 cut(s) | 97, 142, 184, 203 |
| BstSFI | CTRYAG | 1 cut(s) | 146 |
| BstX2I | RGATCY | 1 cut(s) | 142 |
| BstYI | RGATCY | 1 cut(s) | 142 |
| BtgI | CCRYGG | 1 cut(s) | 264 |
| BtrI | CACGTC | 2 cut(s) | 59, 69 |
| BtsCI | GGATG | 1 cut(s) | 291 |
| Cac8I | GCNNGC | 1 cut(s) | 318 |
| CviAII | CATG | 2 cut(s) | 265, 299 |
| CviJI | RGCY | 3 cut(s) | 158, 191, 260 |
| CviKI_1 | RGCY | 3 cut(s) | 158, 191, 260 |
| DpnI | GATC | 4 cut(s) | 99, 144, 186, 205 |
| DpnII | GATC | 4 cut(s) | 97, 142, 184, 203 |
| DraIII | CACNNNGTG | 1 cut(s) | 116 |
| Eco130I | CCWWGG | 1 cut(s) | 264 |
| EcoRI | GAATTC | 1 cut(s) | 135 |
| EcoT14I | CCWWGG | 1 cut(s) | 264 |
| ErhI | CCWWGG | 1 cut(s) | 264 |
| FaeI | CATG | 2 cut(s) | 268, 302 |
| FaiI | YATR | 9 cut(s) | 86, 167, 208, 210, 212, 247, 249, 266, 300 |
| FaqI | GGGAC | 3 cut(s) | 62, 256, 297 |
| FatI | CATG | 2 cut(s) | 264, 298 |
| FauI | CCCGC | 1 cut(s) | 21 |
| FauNDI | CATATG | 1 cut(s) | 247 |
| FblI | GTMKAC | 2 cut(s) | 223, 270 |
| Fnu4HI | GCNGC | 2 cut(s) | 261, 329 |
| FokI | GGATG | 1 cut(s) | 278 |
| Fsp4HI | GCNGC | 2 cut(s) | 261, 329 |
| FspBI | CTAG | 2 cut(s) | 140, 188 |
| GluI | GCNGC | 2 cut(s) | 261, 329 |
| Hin1I | GRCGYC | 1 cut(s) | 304 |
| Hin1II | CATG | 2 cut(s) | 268, 302 |
| Hpy166II | GTNNAC | 2 cut(s) | 224, 271 |
| Hpy188I | TCNGA | 1 cut(s) | 184 |
| Hpy188III | TCNNGA | 3 cut(s) | 47, 95, 140 |
| Hpy8I | GTNNAC | 2 cut(s) | 224, 271 |
| Hpy99I | CGWCG | 1 cut(s) | 60 |
| HpyCH4III | ACNGT | 1 cut(s) | 43 |
| HpyCH4IV | ACGT | 5 cut(s) | 36, 58, 68, 174, 304 |
| HpyCH4V | TGCA | 2 cut(s) | 316, 328 |
| HpySE526I | ACGT | 5 cut(s) | 36, 58, 68, 174, 304 |
| Hsp92I | GRCGYC | 1 cut(s) | 304 |
| Hsp92II | CATG | 2 cut(s) | 268, 302 |
| Kzo9I | GATC | 4 cut(s) | 97, 142, 184, 203 |
| LpnPI | CCDG | 7 cut(s) | 60, 183, 220, 225, 238, 266, 302 |
| MaeI | CTAG | 2 cut(s) | 140, 188 |
| MaeII | ACGT | 5 cut(s) | 36, 58, 68, 174, 304 |
| MaeIII | GTNAC | 2 cut(s) | 37, 196 |
| MalI | GATC | 4 cut(s) | 99, 144, 186, 205 |
| MboI | GATC | 4 cut(s) | 97, 142, 184, 203 |
| MflI | RGATCY | 1 cut(s) | 142 |
| MluCI | AATT | 2 cut(s) | 62, 135 |
| MnlI | CCTC | 2 cut(s) | 237, 284 |
| NcoI | CCATGG | 1 cut(s) | 264 |
| NdeI | CATATG | 1 cut(s) | 247 |
| NdeII | GATC | 4 cut(s) | 97, 142, 184, 203 |
| NlaIII | CATG | 2 cut(s) | 268, 302 |
| NmuCI | GTSAC | 1 cut(s) | 196 |
| PkrI | GCNGC | 2 cut(s) | 262, 330 |
| Psp1406I | AACGTT | 1 cut(s) | 36 |
| PsuI | RGATCY | 1 cut(s) | 142 |
| SatI | GCNGC | 2 cut(s) | 261, 329 |
| Sau3AI | GATC | 4 cut(s) | 97, 142, 184, 203 |
| SetI | ASST | 9 cut(s) | 39, 61, 71, 177, 193, 217, 229, 276, 307 |
| SfcI | CTRYAG | 1 cut(s) | 146 |
| Sse9I | AATT | 2 cut(s) | 62, 135 |
| SsiI | CCGC | 4 cut(s) | 14, 108, 130, 261 |
| SspMI | CTAG | 2 cut(s) | 140, 188 |
| StyI | CCWWGG | 1 cut(s) | 264 |
| TaaI | ACNGT | 1 cut(s) | 43 |
| TaiI | ACGT | 5 cut(s) | 39, 61, 71, 177, 307 |
| TasI | AATT | 2 cut(s) | 62, 135 |
| TauI | GCSGC | 1 cut(s) | 263 |
| TseFI | GTSAC | 1 cut(s) | 196 |
| TseI | GCWGC | 1 cut(s) | 328 |
| Tsp45I | GTSAC | 1 cut(s) | 196 |
| TspDTI | ATGAA | 2 cut(s) | 66, 138 |
| XapI | RAATTY | 1 cut(s) | 135 |
| XbaI | TCTAGA | 1 cut(s) | 139 |
| XmiI | GTMKAC | 2 cut(s) | 223, 270 |
| XspI | CTAG | 2 cut(s) | 140, 188 |
| ZraI | GACGTC | 1 cut(s) | 305 |