aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 58, 104, 223 |
| AclWI | GGATC | 2 cut(s) | 212, 515 |
| AcsI | RAATTY | 3 cut(s) | 182, 544, 770 |
| AdeI | CACNNNGTG | 2 cut(s) | 236, 416 |
| AfaI | GTAC | 2 cut(s) | 25, 602 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 64, 373, 374, 375 |
| AgsI | TTSAA | 2 cut(s) | 617, 776 |
| AjnI | CCWGG | 1 cut(s) | 813 |
| AluBI | AGCT | 1 cut(s) | 338 |
| AluI | AGCT | 1 cut(s) | 338 |
| Alw21I | GWGCWC | 1 cut(s) | 741 |
| Alw26I | GTCTC | 3 cut(s) | 7, 213, 568 |
| AlwI | GGATC | 2 cut(s) | 212, 515 |
| AoxI | GGCC | 2 cut(s) | 461, 791 |
| ApeKI | GCWGC | 2 cut(s) | 338, 386 |
| ApoI | RAATTY | 3 cut(s) | 182, 544, 770 |
| AseI | ATTAAT | 1 cut(s) | 527 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 621 |
| AspS9I | GGNCC | 1 cut(s) | 5 |
| AsuHPI | GGTGA | 2 cut(s) | 92, 476 |
| AvaII | GGWCC | 1 cut(s) | 5 |
| BaeGI | GKGCMC | 1 cut(s) | 381 |
| BbsI | GAAGAC | 1 cut(s) | 121 |
| Bbv12I | GWGCWC | 1 cut(s) | 741 |
| BbvI | GCAGC | 2 cut(s) | 325, 373 |
| BciT130I | CCWGG | 1 cut(s) | 815 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BcoDI | GTCTC | 3 cut(s) | 7, 213, 568 |
| BfaI | CTAG | 1 cut(s) | 96 |
| BfmI | CTRYAG | 2 cut(s) | 351, 387 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BisI | GCNGC | 2 cut(s) | 339, 387 |
| BlsI | GCNGC | 2 cut(s) | 340, 388 |
| BmcAI | AGTACT | 1 cut(s) | 25 |
| Bme1390I | CCNGG | 1 cut(s) | 815 |
| Bme18I | GGWCC | 1 cut(s) | 5 |
| BmgT120I | GGNCC | 1 cut(s) | 5 |
| BmiI | GGNNCC | 1 cut(s) | 6 |
| BmrFI | CCNGG | 1 cut(s) | 815 |
| BmsI | GCATC | 1 cut(s) | 276 |
| BpiI | GAAGAC | 1 cut(s) | 121 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 533, 565, 548, 580 |
| BpmI | CTGGAG | 1 cut(s) | 617 |
| BpuEI | CTTGAG | 1 cut(s) | 708 |
| Bsa29I | ATCGAT | 1 cut(s) | 261 |
| BsaBI | GATNNNNATC | 1 cut(s) | 40 |
| BsaJI | CCNNGG | 2 cut(s) | 167, 488 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 411, 441 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 64, 373, 374, 375 |
| Bse1I | ACTGG | 1 cut(s) | 600 |
| Bse8I | GATNNNNATC | 1 cut(s) | 40 |
| BseBI | CCWGG | 1 cut(s) | 815 |
| BseCI | ATCGAT | 1 cut(s) | 261 |
| BseDI | CCNNGG | 2 cut(s) | 167, 488 |
| BseJI | GATNNNNATC | 1 cut(s) | 40 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 64, 373, 374, 375 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseNI | ACTGG | 1 cut(s) | 600 |
| BseSI | GKGCMC | 1 cut(s) | 381 |
| BseXI | GCAGC | 2 cut(s) | 325, 373 |
| BshFI | GGCC | 2 cut(s) | 463, 793 |
| BshVI | ATCGAT | 1 cut(s) | 261 |
| BsiHKAI | GWGCWC | 1 cut(s) | 741 |
| BslFI | GGGAC | 2 cut(s) | 182, 350 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 64, 373, 374, 375 |
| BsmAI | GTCTC | 3 cut(s) | 7, 213, 568 |
| BsmBI | CGTCTC | 1 cut(s) | 7 |
| BsmFI | GGGAC | 2 cut(s) | 182, 350 |
| BsnI | GGCC | 2 cut(s) | 463, 793 |
| Bsp1286I | GDGCHC | 2 cut(s) | 381, 741 |
| Bsp143I | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| Bsp19I | CCATGG | 1 cut(s) | 488 |
| BspACI | CCGC | 3 cut(s) | 58, 104, 223 |
| BspANI | GGCC | 2 cut(s) | 463, 793 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspDI | ATCGAT | 1 cut(s) | 261 |
| BspHI | TCATGA | 2 cut(s) | 553, 583 |
| BspLI | GGNNCC | 1 cut(s) | 6 |
| BspMAI | CTGCAG | 2 cut(s) | 355, 391 |
| BspPI | GGATC | 2 cut(s) | 212, 515 |
| BsrI | ACTGG | 1 cut(s) | 600 |
| BssECI | CCNNGG | 2 cut(s) | 167, 488 |
| BssMI | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| BssT1I | CCWWGG | 2 cut(s) | 167, 488 |
| Bst2UI | CCWGG | 1 cut(s) | 815 |
| Bst4CI | ACNGT | 1 cut(s) | 383 |
| Bst6I | CTCTTC | 2 cut(s) | 446, 491 |
| BstC8I | GCNNGC | 1 cut(s) | 351 |
| BstDEI | CTNAG | 2 cut(s) | 9, 413 |
| BstDSI | CCRYGG | 1 cut(s) | 488 |
| BstKTI | GATC | 5 cut(s) | 85, 220, 265, 326, 523 |
| BstMAI | GTCTC | 3 cut(s) | 7, 213, 568 |
| BstMBI | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 101 |
| BstNI | CCWGG | 1 cut(s) | 815 |
| BstSCI | CCNGG | 1 cut(s) | 813 |
| BstSFI | CTRYAG | 2 cut(s) | 351, 387 |
| BstSLI | GKGCMC | 1 cut(s) | 381 |
| BstV1I | GCAGC | 2 cut(s) | 325, 373 |
| BstV2I | GAAGAC | 1 cut(s) | 121 |
| Bsu15I | ATCGAT | 1 cut(s) | 261 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuRI | GGCC | 2 cut(s) | 463, 793 |
| BsuTUI | ATCGAT | 1 cut(s) | 261 |
| BtgI | CCRYGG | 1 cut(s) | 488 |
| BtsI | GCAGTG | 1 cut(s) | 441 |
| BtsIMutI | CAGTG | 1 cut(s) | 441 |
| Cac8I | GCNNGC | 1 cut(s) | 351 |
| CciI | TCATGA | 2 cut(s) | 553, 583 |
| Cfr13I | GGNCC | 1 cut(s) | 5 |
| ClaI | ATCGAT | 1 cut(s) | 261 |
| Csp6I | GTAC | 2 cut(s) | 24, 601 |
| CviAII | CATG | 3 cut(s) | 489, 554, 584 |
| CviJI | RGCY | 3 cut(s) | 338, 463, 793 |
| CviKI_1 | RGCY | 3 cut(s) | 338, 463, 793 |
| CviQI | GTAC | 2 cut(s) | 24, 601 |
| DdeI | CTNAG | 2 cut(s) | 9, 413 |
| DpnI | GATC | 5 cut(s) | 84, 219, 264, 325, 522 |
| DpnII | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| DraIII | CACNNNGTG | 2 cut(s) | 236, 416 |
| Eam1104I | CTCTTC | 2 cut(s) | 446, 491 |
| EarI | CTCTTC | 2 cut(s) | 446, 491 |
| Eco130I | CCWWGG | 2 cut(s) | 167, 488 |
| Eco32I | GATATC | 2 cut(s) | 307, 748 |
| Eco47I | GGWCC | 1 cut(s) | 5 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5 |
| EcoRI | GAATTC | 1 cut(s) | 544 |
| EcoRII | CCWGG | 1 cut(s) | 813 |
| EcoRV | GATATC | 2 cut(s) | 307, 748 |
| EcoT14I | CCWWGG | 2 cut(s) | 167, 488 |
| ErhI | CCWWGG | 2 cut(s) | 167, 488 |
| Esp3I | CGTCTC | 1 cut(s) | 7 |
| FaeI | CATG | 3 cut(s) | 492, 557, 587 |
| FaqI | GGGAC | 2 cut(s) | 182, 350 |
| FatI | CATG | 3 cut(s) | 488, 553, 583 |
| FauI | CCCGC | 1 cut(s) | 230 |
| Fnu4HI | GCNGC | 2 cut(s) | 339, 387 |
| Fsp4HI | GCNGC | 2 cut(s) | 339, 387 |
| FspBI | CTAG | 1 cut(s) | 96 |
| GluI | GCNGC | 2 cut(s) | 339, 387 |
| GsuI | CTGGAG | 1 cut(s) | 617 |
| HaeIII | GGCC | 2 cut(s) | 463, 793 |
| Hin1II | CATG | 3 cut(s) | 492, 557, 587 |
| HinfI | GANTC | 5 cut(s) | 41, 68, 281, 587, 622 |
| HphI | GGTGA | 2 cut(s) | 92, 476 |
| Hpy188I | TCNGA | 3 cut(s) | 12, 568, 609 |
| Hpy188III | TCNNGA | 4 cut(s) | 554, 584, 626, 725 |
| HpyCH4III | ACNGT | 1 cut(s) | 383 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 5 cut(s) | 341, 353, 389, 710, 767 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 101 |
| HpyF3I | CTNAG | 2 cut(s) | 9, 413 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92II | CATG | 3 cut(s) | 492, 557, 587 |
| Kzo9I | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| LmnI | GCTCC | 1 cut(s) | 610 |
| LpnPI | CCDG | 8 cut(s) | 63, 164, 303, 339, 363, 581, 639, 800 |
| Lsp1109I | GCAGC | 2 cut(s) | 325, 373 |
| LweI | GCATC | 1 cut(s) | 276 |
| MaeI | CTAG | 1 cut(s) | 96 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 1 cut(s) | 316 |
| MalI | GATC | 5 cut(s) | 84, 219, 264, 325, 522 |
| MboI | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| MboII | GAAGA | 5 cut(s) | 121, 257, 463, 505, 508 |
| MhlI | GDGCHC | 2 cut(s) | 381, 741 |
| MluCI | AATT | 3 cut(s) | 182, 544, 770 |
| MmeI | TCCRAC | 2 cut(s) | 13, 71 |
| MnlI | CCTC | 8 cut(s) | 18, 389, 413, 424, 447, 453, 492, 559 |
| MroXI | GAANNNNTTC | 1 cut(s) | 621 |
| MseI | TTAA | 3 cut(s) | 20, 140, 527 |
| MslI | CAYNNNNRTG | 2 cut(s) | 117, 696 |
| MspR9I | CCNGG | 1 cut(s) | 815 |
| MvaI | CCWGG | 1 cut(s) | 815 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 101 |
| NcoI | CCATGG | 1 cut(s) | 488 |
| NdeII | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| NlaIII | CATG | 3 cut(s) | 492, 557, 587 |
| NlaIV | GGNNCC | 1 cut(s) | 6 |
| NmuCI | GTSAC | 1 cut(s) | 316 |
| PagI | TCATGA | 2 cut(s) | 553, 583 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 747 |
| PdmI | GAANNNNTTC | 1 cut(s) | 621 |
| PfeI | GAWTC | 5 cut(s) | 41, 68, 281, 587, 622 |
| PkrI | GCNGC | 2 cut(s) | 340, 388 |
| PpuMI | RGGWCCY | 1 cut(s) | 5 |
| PshBI | ATTAAT | 1 cut(s) | 527 |
| Psp5II | RGGWCCY | 1 cut(s) | 5 |
| Psp6I | CCWGG | 1 cut(s) | 813 |
| PspGI | CCWGG | 1 cut(s) | 813 |
| PspN4I | GGNNCC | 1 cut(s) | 6 |
| PspPI | GGNCC | 1 cut(s) | 5 |
| PspPPI | RGGWCCY | 1 cut(s) | 5 |
| PstI | CTGCAG | 2 cut(s) | 355, 391 |
| RsaI | GTAC | 2 cut(s) | 25, 602 |
| RsaNI | GTAC | 2 cut(s) | 24, 601 |
| RseI | CAYNNNNRTG | 2 cut(s) | 117, 696 |
| SaqAI | TTAA | 3 cut(s) | 20, 140, 527 |
| SatI | GCNGC | 2 cut(s) | 339, 387 |
| Sau3AI | GATC | 5 cut(s) | 82, 217, 262, 323, 520 |
| Sau96I | GGNCC | 1 cut(s) | 5 |
| SbfI | CCTGCAGG | 1 cut(s) | 355 |
| ScaI | AGTACT | 1 cut(s) | 25 |
| ScrFI | CCNGG | 1 cut(s) | 815 |
| SdaI | CCTGCAGG | 1 cut(s) | 355 |
| SduI | GDGCHC | 2 cut(s) | 381, 741 |
| SetI | ASST | 4 cut(s) | 82, 276, 340, 424 |
| SfaNI | GCATC | 1 cut(s) | 276 |
| SfcI | CTRYAG | 2 cut(s) | 351, 387 |
| SinI | GGWCC | 1 cut(s) | 5 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 117, 696 |
| SmlI | CTYRAG | 1 cut(s) | 723 |
| SmoI | CTYRAG | 1 cut(s) | 723 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 355 |
| Sse9I | AATT | 3 cut(s) | 182, 544, 770 |
| SsiI | CCGC | 3 cut(s) | 58, 104, 223 |
| SspMI | CTAG | 1 cut(s) | 96 |
| StyD4I | CCNGG | 1 cut(s) | 813 |
| StyI | CCWWGG | 2 cut(s) | 167, 488 |
| TaaI | ACNGT | 1 cut(s) | 383 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 4 cut(s) | 261, 284, 473, 750 |
| TasI | AATT | 3 cut(s) | 182, 544, 770 |
| TatI | WGTACW | 1 cut(s) | 23 |
| TfiI | GAWTC | 5 cut(s) | 41, 68, 281, 587, 622 |
| Tru1I | TTAA | 3 cut(s) | 20, 140, 527 |
| Tru9I | TTAA | 3 cut(s) | 20, 140, 527 |
| TscAI | CASTG | 1 cut(s) | 441 |
| TseFI | GTSAC | 1 cut(s) | 316 |
| TseI | GCWGC | 2 cut(s) | 338, 386 |
| Tsp45I | GTSAC | 1 cut(s) | 316 |
| TspDTI | ATGAA | 3 cut(s) | 257, 572, 600 |
| TspRI | CASTG | 1 cut(s) | 441 |
| VpaK11BI | GGWCC | 1 cut(s) | 5 |
| VspI | ATTAAT | 1 cut(s) | 527 |
| XapI | RAATTY | 3 cut(s) | 182, 544, 770 |
| XmnI | GAANNNNTTC | 1 cut(s) | 621 |
| XspI | CTAG | 1 cut(s) | 96 |
| ZrmI | AGTACT | 1 cut(s) | 25 |