aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 93, 104, 223, 408 |
| AclWI | GGATC | 1 cut(s) | 212 |
| AcuI | CTGAAG | 1 cut(s) | 251 |
| AdeI | CACNNNGTG | 1 cut(s) | 416 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 77, 140, 374, 375 |
| Alw26I | GTCTC | 3 cut(s) | 7, 116, 213 |
| AlwI | GGATC | 1 cut(s) | 212 |
| AsuHPI | GGTGA | 1 cut(s) | 475 |
| BbsI | GAAGAC | 1 cut(s) | 121 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BcoDI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BfaI | CTAG | 1 cut(s) | 96 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BmiI | GGNNCC | 2 cut(s) | 69, 441 |
| BmsI | GCATC | 3 cut(s) | 276, 283, 328 |
| BpiI | GAAGAC | 1 cut(s) | 121 |
| Bsa29I | ATCGAT | 1 cut(s) | 261 |
| BsaBI | GATNNNNATC | 1 cut(s) | 40 |
| BsaJI | CCNNGG | 1 cut(s) | 445 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 411, 441 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 77, 140, 374, 375 |
| Bse8I | GATNNNNATC | 1 cut(s) | 40 |
| BseCI | ATCGAT | 1 cut(s) | 261 |
| BseDI | CCNNGG | 1 cut(s) | 445 |
| BseJI | GATNNNNATC | 1 cut(s) | 40 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 77, 140, 374, 375 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BshVI | ATCGAT | 1 cut(s) | 261 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 77, 140, 374, 375 |
| BsmAI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BsmBI | CGTCTC | 1 cut(s) | 7 |
| Bsp143I | GATC | 3 cut(s) | 217, 262, 323 |
| BspACI | CCGC | 4 cut(s) | 93, 104, 223, 408 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspDI | ATCGAT | 1 cut(s) | 261 |
| BspLI | GGNNCC | 2 cut(s) | 69, 441 |
| BspPI | GGATC | 1 cut(s) | 212 |
| BssECI | CCNNGG | 1 cut(s) | 445 |
| BssMI | GATC | 3 cut(s) | 217, 262, 323 |
| BssT1I | CCWWGG | 1 cut(s) | 445 |
| Bst4CI | ACNGT | 1 cut(s) | 127 |
| Bst6I | CTCTTC | 1 cut(s) | 445 |
| BstC8I | GCNNGC | 1 cut(s) | 351 |
| BstDEI | CTNAG | 3 cut(s) | 9, 167, 413 |
| BstKTI | GATC | 3 cut(s) | 220, 265, 326 |
| BstMAI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BstMBI | GATC | 3 cut(s) | 217, 262, 323 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 101 |
| BstV2I | GAAGAC | 1 cut(s) | 121 |
| Bsu15I | ATCGAT | 1 cut(s) | 261 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuTUI | ATCGAT | 1 cut(s) | 261 |
| BtsI | GCAGTG | 1 cut(s) | 399 |
| BtsIMutI | CAGTG | 1 cut(s) | 399 |
| Cac8I | GCNNGC | 1 cut(s) | 351 |
| ClaI | ATCGAT | 1 cut(s) | 261 |
| CviAII | CATG | 1 cut(s) | 380 |
| CviJI | RGCY | 2 cut(s) | 434, 440 |
| CviKI_1 | RGCY | 2 cut(s) | 434, 440 |
| DdeI | CTNAG | 3 cut(s) | 9, 167, 413 |
| DpnI | GATC | 3 cut(s) | 219, 264, 325 |
| DpnII | GATC | 3 cut(s) | 217, 262, 323 |
| DraIII | CACNNNGTG | 1 cut(s) | 416 |
| Eam1104I | CTCTTC | 1 cut(s) | 445 |
| EarI | CTCTTC | 1 cut(s) | 445 |
| Eco130I | CCWWGG | 1 cut(s) | 445 |
| Eco32I | GATATC | 1 cut(s) | 307 |
| Eco57I | CTGAAG | 1 cut(s) | 251 |
| EcoRV | GATATC | 1 cut(s) | 307 |
| EcoT14I | CCWWGG | 1 cut(s) | 445 |
| ErhI | CCWWGG | 1 cut(s) | 445 |
| Esp3I | CGTCTC | 1 cut(s) | 7 |
| FaeI | CATG | 1 cut(s) | 383 |
| FaiI | YATR | 5 cut(s) | 30, 330, 332, 381, 437 |
| FatI | CATG | 1 cut(s) | 379 |
| FauI | CCCGC | 1 cut(s) | 230 |
| FspBI | CTAG | 1 cut(s) | 96 |
| Hin1II | CATG | 1 cut(s) | 383 |
| HinfI | GANTC | 3 cut(s) | 4, 41, 281 |
| HphI | GGTGA | 1 cut(s) | 475 |
| Hpy166II | GTNNAC | 1 cut(s) | 300 |
| Hpy188I | TCNGA | 2 cut(s) | 12, 270 |
| Hpy8I | GTNNAC | 1 cut(s) | 300 |
| Hpy99I | CGWCG | 1 cut(s) | 139 |
| HpyCH4III | ACNGT | 1 cut(s) | 127 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 3 cut(s) | 341, 353, 404 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 101 |
| HpyF3I | CTNAG | 3 cut(s) | 9, 167, 413 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92II | CATG | 1 cut(s) | 383 |
| Kzo9I | GATC | 3 cut(s) | 217, 262, 323 |
| LmnI | GCTCC | 1 cut(s) | 445 |
| LpnPI | CCDG | 4 cut(s) | 164, 303, 348, 363 |
| LweI | GCATC | 3 cut(s) | 276, 283, 328 |
| MaeI | CTAG | 1 cut(s) | 96 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 4 cut(s) | 110, 316, 385, 461 |
| MalI | GATC | 3 cut(s) | 219, 264, 325 |
| MboI | GATC | 3 cut(s) | 217, 262, 323 |
| MboII | GAAGA | 3 cut(s) | 121, 257, 462 |
| MluCI | AATT | 2 cut(s) | 51, 182 |
| MlyI | GAGTC | 1 cut(s) | 13 |
| MmeI | TCCRAC | 1 cut(s) | 13 |
| MnlI | CCTC | 6 cut(s) | 18, 389, 413, 424, 446, 452 |
| MseI | TTAA | 1 cut(s) | 20 |
| MslI | CAYNNNNRTG | 1 cut(s) | 117 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 101 |
| NdeII | GATC | 3 cut(s) | 217, 262, 323 |
| NlaIII | CATG | 1 cut(s) | 383 |
| NlaIV | GGNNCC | 2 cut(s) | 69, 441 |
| NmuCI | GTSAC | 4 cut(s) | 110, 316, 385, 461 |
| PfeI | GAWTC | 2 cut(s) | 41, 281 |
| PleI | GAGTC | 1 cut(s) | 12 |
| PpsI | GAGTC | 1 cut(s) | 12 |
| PspN4I | GGNNCC | 2 cut(s) | 69, 441 |
| RseI | CAYNNNNRTG | 1 cut(s) | 117 |
| SaqAI | TTAA | 1 cut(s) | 20 |
| Sau3AI | GATC | 3 cut(s) | 217, 262, 323 |
| SchI | GAGTC | 1 cut(s) | 13 |
| SetI | ASST | 5 cut(s) | 276, 359, 424, 463, 488 |
| SfaNI | GCATC | 3 cut(s) | 276, 283, 328 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 117 |
| Sse9I | AATT | 2 cut(s) | 51, 182 |
| SsiI | CCGC | 4 cut(s) | 93, 104, 223, 408 |
| SspMI | CTAG | 1 cut(s) | 96 |
| StyI | CCWWGG | 1 cut(s) | 445 |
| TaaI | ACNGT | 1 cut(s) | 127 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 3 cut(s) | 261, 284, 472 |
| TasI | AATT | 2 cut(s) | 51, 182 |
| TfiI | GAWTC | 2 cut(s) | 41, 281 |
| Tru1I | TTAA | 1 cut(s) | 20 |
| Tru9I | TTAA | 1 cut(s) | 20 |
| TscAI | CASTG | 1 cut(s) | 406 |
| TseFI | GTSAC | 4 cut(s) | 110, 316, 385, 461 |
| Tsp45I | GTSAC | 4 cut(s) | 110, 316, 385, 461 |
| TspRI | CASTG | 1 cut(s) | 406 |
| XspI | CTAG | 1 cut(s) | 96 |