aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 248 |
| AccI | GTMKAC | 1 cut(s) | 142 |
| AciI | CCGC | 1 cut(s) | 104 |
| AclWI | GGATC | 1 cut(s) | 249 |
| AcoI | YGGCCR | 1 cut(s) | 105 |
| AcuI | CTGAAG | 1 cut(s) | 152 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 152 |
| AgsI | TTSAA | 1 cut(s) | 159 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 216, 248 |
| AlwI | GGATC | 1 cut(s) | 249 |
| AoxI | GGCC | 1 cut(s) | 105 |
| AseI | ATTAAT | 2 cut(s) | 75, 246 |
| BccI | CCATC | 1 cut(s) | 124 |
| BfaI | CTAG | 1 cut(s) | 174 |
| BfmI | CTRYAG | 1 cut(s) | 279 |
| BisI | GCNGC | 1 cut(s) | 105 |
| BlsI | GCNGC | 1 cut(s) | 106 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 152 |
| Bse118I | RCCGGY | 1 cut(s) | 64 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 152 |
| BseYI | CCCAGC | 1 cut(s) | 49 |
| BsgI | GTGCAG | 1 cut(s) | 108 |
| BshFI | GGCC | 1 cut(s) | 107 |
| BsiSI | CCGG | 1 cut(s) | 65 |
| BslFI | GGGAC | 1 cut(s) | 65 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 152 |
| BsmFI | GGGAC | 1 cut(s) | 65 |
| BsmI | GAATGC | 1 cut(s) | 11 |
| BsnI | GGCC | 1 cut(s) | 107 |
| Bsp143I | GATC | 2 cut(s) | 38, 254 |
| BspACI | CCGC | 1 cut(s) | 104 |
| BspANI | GGCC | 1 cut(s) | 107 |
| BspHI | TCATGA | 1 cut(s) | 265 |
| BspPI | GGATC | 1 cut(s) | 249 |
| BsrFI | RCCGGY | 1 cut(s) | 64 |
| BssAI | RCCGGY | 1 cut(s) | 64 |
| BssMI | GATC | 2 cut(s) | 38, 254 |
| Bst6I | CTCTTC | 2 cut(s) | 219, 279 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 59 |
| BstC8I | GCNNGC | 1 cut(s) | 127 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 150 |
| BstKTI | GATC | 2 cut(s) | 41, 257 |
| BstMBI | GATC | 2 cut(s) | 38, 254 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 59 |
| BstSFI | CTRYAG | 1 cut(s) | 279 |
| BsuRI | GGCC | 1 cut(s) | 107 |
| BtsIMutI | CAGTG | 1 cut(s) | 15 |
| Cac8I | GCNNGC | 1 cut(s) | 127 |
| CciI | TCATGA | 1 cut(s) | 265 |
| Cfr10I | RCCGGY | 1 cut(s) | 64 |
| CviAII | CATG | 2 cut(s) | 221, 266 |
| CviJI | RGCY | 2 cut(s) | 107, 115 |
| CviKI_1 | RGCY | 2 cut(s) | 107, 115 |
| DpnI | GATC | 2 cut(s) | 40, 256 |
| DpnII | GATC | 2 cut(s) | 38, 254 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 248 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 248 |
| EaeI | YGGCCR | 1 cut(s) | 105 |
| Eam1104I | CTCTTC | 2 cut(s) | 219, 279 |
| EarI | CTCTTC | 2 cut(s) | 219, 279 |
| Eco32I | GATATC | 1 cut(s) | 214 |
| Eco57I | CTGAAG | 1 cut(s) | 152 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 150 |
| EcoRV | GATATC | 1 cut(s) | 214 |
| EcoT22I | ATGCAT | 1 cut(s) | 13 |
| FaeI | CATG | 2 cut(s) | 224, 269 |
| FaiI | YATR | 3 cut(s) | 45, 222, 267 |
| FaqI | GGGAC | 1 cut(s) | 65 |
| FatI | CATG | 2 cut(s) | 220, 265 |
| FblI | GTMKAC | 1 cut(s) | 142 |
| Fnu4HI | GCNGC | 1 cut(s) | 105 |
| Fsp4HI | GCNGC | 1 cut(s) | 105 |
| FspBI | CTAG | 1 cut(s) | 174 |
| GluI | GCNGC | 1 cut(s) | 105 |
| GsaI | CCCAGC | 1 cut(s) | 53 |
| HaeIII | GGCC | 1 cut(s) | 107 |
| HapII | CCGG | 1 cut(s) | 65 |
| Hin1II | CATG | 2 cut(s) | 224, 269 |
| HinfI | GANTC | 3 cut(s) | 164, 202, 269 |
| HpaII | CCGG | 1 cut(s) | 65 |
| Hpy166II | GTNNAC | 1 cut(s) | 143 |
| Hpy188I | TCNGA | 2 cut(s) | 201, 254 |
| Hpy188III | TCNNGA | 1 cut(s) | 266 |
| Hpy8I | GTNNAC | 1 cut(s) | 143 |
| HpyCH4IV | ACGT | 1 cut(s) | 150 |
| HpyCH4V | TGCA | 3 cut(s) | 11, 62, 125 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 59 |
| HpySE526I | ACGT | 1 cut(s) | 150 |
| Hsp92II | CATG | 2 cut(s) | 224, 269 |
| Kzo9I | GATC | 2 cut(s) | 38, 254 |
| LpnPI | CCDG | 5 cut(s) | 18, 63, 78, 143, 159 |
| MaeI | CTAG | 1 cut(s) | 174 |
| MaeII | ACGT | 1 cut(s) | 150 |
| MaeIII | GTNAC | 2 cut(s) | 31, 274 |
| MalI | GATC | 2 cut(s) | 40, 256 |
| MboI | GATC | 2 cut(s) | 38, 254 |
| MboII | GAAGA | 2 cut(s) | 236, 296 |
| MfeI | CAATTG | 1 cut(s) | 84 |
| MluCI | AATT | 3 cut(s) | 72, 84, 261 |
| MnlI | CCTC | 2 cut(s) | 174, 280 |
| Mph1103I | ATGCAT | 1 cut(s) | 13 |
| MseI | TTAA | 2 cut(s) | 75, 246 |
| MspI | CCGG | 1 cut(s) | 65 |
| MunI | CAATTG | 1 cut(s) | 84 |
| Mva1269I | GAATGC | 1 cut(s) | 11 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 59 |
| NdeII | GATC | 2 cut(s) | 38, 254 |
| NlaIII | CATG | 2 cut(s) | 224, 269 |
| NmuCI | GTSAC | 2 cut(s) | 31, 274 |
| NsiI | ATGCAT | 1 cut(s) | 13 |
| PagI | TCATGA | 1 cut(s) | 265 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 133 |
| PctI | GAATGC | 1 cut(s) | 11 |
| PfeI | GAWTC | 3 cut(s) | 164, 202, 269 |
| PkrI | GCNGC | 1 cut(s) | 106 |
| PshBI | ATTAAT | 2 cut(s) | 75, 246 |
| PspFI | CCCAGC | 1 cut(s) | 49 |
| SaqAI | TTAA | 2 cut(s) | 75, 246 |
| SatI | GCNGC | 1 cut(s) | 105 |
| Sau3AI | GATC | 2 cut(s) | 38, 254 |
| SetI | ASST | 3 cut(s) | 148, 153, 157 |
| SfcI | CTRYAG | 1 cut(s) | 279 |
| Sse9I | AATT | 3 cut(s) | 72, 84, 261 |
| SsiI | CCGC | 1 cut(s) | 104 |
| SspI | AATATT | 1 cut(s) | 232 |
| SspMI | CTAG | 1 cut(s) | 174 |
| TaiI | ACGT | 1 cut(s) | 153 |
| TasI | AATT | 3 cut(s) | 72, 84, 261 |
| TauI | GCSGC | 1 cut(s) | 107 |
| TfiI | GAWTC | 3 cut(s) | 164, 202, 269 |
| Tru1I | TTAA | 2 cut(s) | 75, 246 |
| Tru9I | TTAA | 2 cut(s) | 75, 246 |
| TscAI | CASTG | 1 cut(s) | 22 |
| TseFI | GTSAC | 2 cut(s) | 31, 274 |
| Tsp45I | GTSAC | 2 cut(s) | 31, 274 |
| TspDTI | ATGAA | 2 cut(s) | 237, 254 |
| TspRI | CASTG | 1 cut(s) | 22 |
| VspI | ATTAAT | 2 cut(s) | 75, 246 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 150 |
| XmiI | GTMKAC | 1 cut(s) | 142 |
| XspI | CTAG | 1 cut(s) | 174 |
| Zsp2I | ATGCAT | 1 cut(s) | 13 |