aspartic-type endopeptidase activity
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 278 |
| AccB1I | GGYRCC | 1 cut(s) | 98 |
| AciI | CCGC | 1 cut(s) | 102 |
| AcsI | RAATTY | 1 cut(s) | 422 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 88, 89, 90 |
| AgeI | ACCGGT | 1 cut(s) | 95 |
| AgsI | TTSAA | 2 cut(s) | 335, 470 |
| AluBI | AGCT | 1 cut(s) | 53 |
| AluI | AGCT | 1 cut(s) | 53 |
| Alw21I | GWGCWC | 1 cut(s) | 459 |
| Alw26I | GTCTC | 1 cut(s) | 286 |
| AoxI | GGCC | 3 cut(s) | 147, 176, 509 |
| ApeKI | GCWGC | 1 cut(s) | 53 |
| ApoI | RAATTY | 1 cut(s) | 422 |
| AseI | ATTAAT | 1 cut(s) | 242 |
| AsiGI | ACCGGT | 1 cut(s) | 95 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 339 |
| AspS9I | GGNCC | 1 cut(s) | 93 |
| AsuHPI | GGTGA | 1 cut(s) | 191 |
| AvaII | GGWCC | 1 cut(s) | 93 |
| BanI | GGYRCC | 1 cut(s) | 98 |
| Bbv12I | GWGCWC | 1 cut(s) | 459 |
| BbvI | GCAGC | 1 cut(s) | 40 |
| BceAI | ACGGC | 1 cut(s) | 134 |
| BcoDI | GTCTC | 1 cut(s) | 286 |
| BfaI | CTAG | 1 cut(s) | 532 |
| BglII | AGATCT | 1 cut(s) | 398 |
| BisI | GCNGC | 2 cut(s) | 54, 102 |
| BlsI | GCNGC | 2 cut(s) | 55, 103 |
| Bme18I | GGWCC | 1 cut(s) | 93 |
| BmgT120I | GGNCC | 1 cut(s) | 93 |
| BmiI | GGNNCC | 3 cut(s) | 94, 100, 156 |
| BmsI | GCATC | 3 cut(s) | 310, 314, 514 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 248, 280 |
| BpmI | CTGGAG | 1 cut(s) | 335 |
| BpuEI | CTTGAG | 1 cut(s) | 426 |
| BsaJI | CCNNGG | 2 cut(s) | 150, 203 |
| BsaWI | WCCGGW | 1 cut(s) | 95 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 126, 156 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 88, 89, 90 |
| Bse118I | RCCGGY | 1 cut(s) | 95 |
| Bse1I | ACTGG | 1 cut(s) | 318 |
| BseDI | CCNNGG | 2 cut(s) | 150, 203 |
| BseGI | GGATG | 2 cut(s) | 222, 325 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 88, 89, 90 |
| BseNI | ACTGG | 1 cut(s) | 318 |
| BseRI | GAGGAG | 1 cut(s) | 147 |
| BseXI | GCAGC | 1 cut(s) | 40 |
| BshFI | GGCC | 3 cut(s) | 149, 178, 511 |
| BshNI | GGYRCC | 1 cut(s) | 98 |
| BshTI | ACCGGT | 1 cut(s) | 95 |
| BsiHKAI | GWGCWC | 1 cut(s) | 459 |
| BsiSI | CCGG | 1 cut(s) | 96 |
| BslFI | GGGAC | 2 cut(s) | 65, 106 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 88, 89, 90 |
| BsmAI | GTCTC | 1 cut(s) | 286 |
| BsmFI | GGGAC | 2 cut(s) | 65, 106 |
| BsnI | GGCC | 3 cut(s) | 149, 178, 511 |
| Bsp1286I | GDGCHC | 1 cut(s) | 459 |
| Bsp143I | GATC | 3 cut(s) | 38, 235, 398 |
| BspACI | CCGC | 1 cut(s) | 102 |
| BspANI | GGCC | 3 cut(s) | 149, 178, 511 |
| BspHI | TCATGA | 1 cut(s) | 268 |
| BspLI | GGNNCC | 3 cut(s) | 94, 100, 156 |
| BspT107I | GGYRCC | 1 cut(s) | 98 |
| BsrFI | RCCGGY | 1 cut(s) | 95 |
| BsrI | ACTGG | 1 cut(s) | 318 |
| BssAI | RCCGGY | 1 cut(s) | 95 |
| BssECI | CCNNGG | 2 cut(s) | 150, 203 |
| BssMI | GATC | 3 cut(s) | 38, 235, 398 |
| BssT1I | CCWWGG | 1 cut(s) | 203 |
| Bst4CI | ACNGT | 2 cut(s) | 293, 436 |
| Bst6I | CTCTTC | 2 cut(s) | 161, 206 |
| BstC8I | GCNNGC | 1 cut(s) | 66 |
| BstDEI | CTNAG | 1 cut(s) | 128 |
| BstDSI | CCRYGG | 1 cut(s) | 150 |
| BstF5I | GGATG | 2 cut(s) | 222, 325 |
| BstKTI | GATC | 3 cut(s) | 41, 238, 401 |
| BstMAI | GTCTC | 1 cut(s) | 286 |
| BstMBI | GATC | 3 cut(s) | 38, 235, 398 |
| BstV1I | GCAGC | 1 cut(s) | 40 |
| BstX2I | RGATCY | 1 cut(s) | 398 |
| BstYI | RGATCY | 1 cut(s) | 398 |
| BsuRI | GGCC | 3 cut(s) | 149, 178, 511 |
| BtgI | CCRYGG | 1 cut(s) | 150 |
| BtsCI | GGATG | 2 cut(s) | 222, 325 |
| BtsI | GCAGTG | 1 cut(s) | 114 |
| BtsIMutI | CAGTG | 2 cut(s) | 114, 432 |
| Cac8I | GCNNGC | 1 cut(s) | 66 |
| CciI | TCATGA | 1 cut(s) | 268 |
| Cfr10I | RCCGGY | 1 cut(s) | 95 |
| Cfr13I | GGNCC | 1 cut(s) | 93 |
| CspAI | ACCGGT | 1 cut(s) | 95 |
| CviAII | CATG | 3 cut(s) | 269, 302, 474 |
| CviJI | RGCY | 5 cut(s) | 53, 149, 155, 178, 511 |
| CviKI_1 | RGCY | 5 cut(s) | 53, 149, 155, 178, 511 |
| DdeI | CTNAG | 1 cut(s) | 128 |
| DpnI | GATC | 3 cut(s) | 40, 237, 400 |
| DpnII | GATC | 3 cut(s) | 38, 235, 398 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 278 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 278 |
| Eam1104I | CTCTTC | 2 cut(s) | 161, 206 |
| EarI | CTCTTC | 2 cut(s) | 161, 206 |
| Eco130I | CCWWGG | 1 cut(s) | 203 |
| Eco32I | GATATC | 1 cut(s) | 22 |
| Eco47I | GGWCC | 1 cut(s) | 93 |
| EcoRV | GATATC | 1 cut(s) | 22 |
| EcoT14I | CCWWGG | 1 cut(s) | 203 |
| EcoT22I | ATGCAT | 2 cut(s) | 13, 307 |
| ErhI | CCWWGG | 1 cut(s) | 203 |
| FaeI | CATG | 3 cut(s) | 272, 305, 477 |
| FaqI | GGGAC | 2 cut(s) | 65, 106 |
| FatI | CATG | 3 cut(s) | 268, 301, 473 |
| Fnu4HI | GCNGC | 2 cut(s) | 54, 102 |
| FokI | GGATG | 2 cut(s) | 229, 332 |
| Fsp4HI | GCNGC | 2 cut(s) | 54, 102 |
| FspBI | CTAG | 1 cut(s) | 532 |
| GluI | GCNGC | 2 cut(s) | 54, 102 |
| GsuI | CTGGAG | 1 cut(s) | 335 |
| HaeIII | GGCC | 3 cut(s) | 149, 178, 511 |
| HapII | CCGG | 1 cut(s) | 96 |
| Hin1II | CATG | 3 cut(s) | 272, 305, 477 |
| HincII | GTYRAC | 1 cut(s) | 494 |
| HindII | GTYRAC | 1 cut(s) | 494 |
| HinfI | GANTC | 2 cut(s) | 259, 340 |
| HpaII | CCGG | 1 cut(s) | 96 |
| HphI | GGTGA | 1 cut(s) | 191 |
| Hpy166II | GTNNAC | 1 cut(s) | 494 |
| Hpy188I | TCNGA | 2 cut(s) | 286, 327 |
| Hpy188III | TCNNGA | 3 cut(s) | 269, 344, 443 |
| Hpy8I | GTNNAC | 1 cut(s) | 494 |
| HpyCH4III | ACNGT | 2 cut(s) | 293, 436 |
| HpyCH4V | TGCA | 7 cut(s) | 11, 56, 68, 119, 305, 485, 527 |
| HpyF3I | CTNAG | 1 cut(s) | 128 |
| Hsp92II | CATG | 3 cut(s) | 272, 305, 477 |
| Kzo9I | GATC | 3 cut(s) | 38, 235, 398 |
| LmnI | GCTCC | 2 cut(s) | 160, 328 |
| LpnPI | CCDG | 6 cut(s) | 18, 63, 78, 109, 299, 357 |
| Lsp1109I | GCAGC | 1 cut(s) | 40 |
| LweI | GCATC | 3 cut(s) | 310, 314, 514 |
| MaeI | CTAG | 1 cut(s) | 532 |
| MalI | GATC | 3 cut(s) | 40, 237, 400 |
| MboI | GATC | 3 cut(s) | 38, 235, 398 |
| MboII | GAAGA | 4 cut(s) | 178, 220, 223, 393 |
| MflI | RGATCY | 1 cut(s) | 398 |
| MhlI | GDGCHC | 1 cut(s) | 459 |
| MluCI | AATT | 3 cut(s) | 29, 276, 422 |
| MnlI | CCTC | 9 cut(s) | 104, 125, 128, 139, 162, 168, 207, 274, 310 |
| Mph1103I | ATGCAT | 2 cut(s) | 13, 307 |
| MroXI | GAANNNNTTC | 1 cut(s) | 339 |
| MseI | TTAA | 1 cut(s) | 242 |
| MslI | CAYNNNNRTG | 1 cut(s) | 414 |
| MspA1I | CMGCKG | 1 cut(s) | 53 |
| MspI | CCGG | 1 cut(s) | 96 |
| NdeII | GATC | 3 cut(s) | 38, 235, 398 |
| NlaIII | CATG | 3 cut(s) | 272, 305, 477 |
| NlaIV | GGNNCC | 3 cut(s) | 94, 100, 156 |
| NsiI | ATGCAT | 2 cut(s) | 13, 307 |
| PagI | TCATGA | 1 cut(s) | 268 |
| PdmI | GAANNNNTTC | 1 cut(s) | 339 |
| PfeI | GAWTC | 2 cut(s) | 259, 340 |
| PinAI | ACCGGT | 1 cut(s) | 95 |
| PkrI | GCNGC | 2 cut(s) | 55, 103 |
| PshBI | ATTAAT | 1 cut(s) | 242 |
| PspN4I | GGNNCC | 3 cut(s) | 94, 100, 156 |
| PspPI | GGNCC | 1 cut(s) | 93 |
| PsuI | RGATCY | 1 cut(s) | 398 |
| PvuII | CAGCTG | 1 cut(s) | 53 |
| RseI | CAYNNNNRTG | 1 cut(s) | 414 |
| SaqAI | TTAA | 1 cut(s) | 242 |
| SatI | GCNGC | 2 cut(s) | 54, 102 |
| Sau3AI | GATC | 3 cut(s) | 38, 235, 398 |
| Sau96I | GGNCC | 1 cut(s) | 93 |
| SduI | GDGCHC | 1 cut(s) | 459 |
| SetI | ASST | 2 cut(s) | 55, 139 |
| SfaNI | GCATC | 3 cut(s) | 310, 314, 514 |
| SinI | GGWCC | 1 cut(s) | 93 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 414 |
| SmlI | CTYRAG | 1 cut(s) | 441 |
| SmoI | CTYRAG | 1 cut(s) | 441 |
| Sse9I | AATT | 3 cut(s) | 29, 276, 422 |
| SsiI | CCGC | 1 cut(s) | 102 |
| SspMI | CTAG | 1 cut(s) | 532 |
| StyI | CCWWGG | 1 cut(s) | 203 |
| TaaI | ACNGT | 2 cut(s) | 293, 436 |
| TaqI | TCGA | 1 cut(s) | 188 |
| TasI | AATT | 3 cut(s) | 29, 276, 422 |
| TauI | GCSGC | 1 cut(s) | 104 |
| TfiI | GAWTC | 2 cut(s) | 259, 340 |
| Tru1I | TTAA | 1 cut(s) | 242 |
| Tru9I | TTAA | 1 cut(s) | 242 |
| TscAI | CASTG | 2 cut(s) | 121, 439 |
| TseI | GCWGC | 1 cut(s) | 53 |
| TspDTI | ATGAA | 2 cut(s) | 233, 435 |
| TspRI | CASTG | 2 cut(s) | 121, 439 |
| VpaK11BI | GGWCC | 1 cut(s) | 93 |
| VspI | ATTAAT | 1 cut(s) | 242 |
| XapI | RAATTY | 1 cut(s) | 422 |
| XmnI | GAANNNNTTC | 1 cut(s) | 339 |
| XspI | CTAG | 1 cut(s) | 532 |
| Zsp2I | ATGCAT | 2 cut(s) | 13, 307 |