aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 449 |
| AciI | CCGC | 4 cut(s) | 124, 159, 170, 289 |
| AclWI | GGATC | 1 cut(s) | 278 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AcyI | GRCGYC | 1 cut(s) | 353 |
| AdeI | CACNNNGTG | 2 cut(s) | 302, 482 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 130, 143, 439, 440, 441 |
| AgsI | TTSAA | 1 cut(s) | 497 |
| AluBI | AGCT | 1 cut(s) | 404 |
| AluI | AGCT | 1 cut(s) | 404 |
| Alw26I | GTCTC | 2 cut(s) | 73, 182 |
| AlwI | GGATC | 1 cut(s) | 278 |
| ApeKI | GCWGC | 1 cut(s) | 404 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| AspLEI | GCGC | 3 cut(s) | 40, 49, 60 |
| AspS9I | GGNCC | 1 cut(s) | 71 |
| AsuHPI | GGTGA | 1 cut(s) | 542 |
| AvaII | GGWCC | 1 cut(s) | 71 |
| BaeGI | GKGCMC | 1 cut(s) | 447 |
| BanI | GGYRCC | 1 cut(s) | 449 |
| BbsI | GAAGAC | 1 cut(s) | 187 |
| BbvI | GCAGC | 1 cut(s) | 391 |
| BceAI | ACGGC | 1 cut(s) | 485 |
| BciVI | GTATCC | 1 cut(s) | 95 |
| BcoDI | GTCTC | 2 cut(s) | 73, 182 |
| BfaI | CTAG | 1 cut(s) | 162 |
| BfuI | GTATCC | 1 cut(s) | 95 |
| BisI | GCNGC | 1 cut(s) | 405 |
| BlsI | GCNGC | 1 cut(s) | 406 |
| Bme18I | GGWCC | 1 cut(s) | 71 |
| BmgT120I | GGNCC | 1 cut(s) | 71 |
| BmiI | GGNNCC | 4 cut(s) | 72, 135, 451, 507 |
| BmsI | GCATC | 1 cut(s) | 349 |
| BpiI | GAAGAC | 1 cut(s) | 187 |
| BsaHI | GRCGYC | 1 cut(s) | 353 |
| BsaJI | CCNNGG | 3 cut(s) | 233, 501, 554 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 477, 507 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 130, 143, 439, 440, 441 |
| BseDI | CCNNGG | 3 cut(s) | 233, 501, 554 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 130, 143, 439, 440, 441 |
| BseMII | CTCAG | 2 cut(s) | 89, 468 |
| BseSI | GKGCMC | 1 cut(s) | 447 |
| BseXI | GCAGC | 1 cut(s) | 391 |
| BshNI | GGYRCC | 1 cut(s) | 449 |
| BslFI | GGGAC | 1 cut(s) | 416 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 130, 143, 439, 440, 441 |
| BsmAI | GTCTC | 2 cut(s) | 73, 182 |
| BsmBI | CGTCTC | 1 cut(s) | 73 |
| BsmFI | GGGAC | 1 cut(s) | 416 |
| Bsp1286I | GDGCHC | 1 cut(s) | 447 |
| Bsp143I | GATC | 3 cut(s) | 148, 283, 389 |
| BspACI | CCGC | 4 cut(s) | 124, 159, 170, 289 |
| BspCNI | CTCAG | 2 cut(s) | 88, 467 |
| BspLI | GGNNCC | 4 cut(s) | 72, 135, 451, 507 |
| BspPI | GGATC | 1 cut(s) | 278 |
| BspT107I | GGYRCC | 1 cut(s) | 449 |
| BssECI | CCNNGG | 3 cut(s) | 233, 501, 554 |
| BssMI | GATC | 3 cut(s) | 148, 283, 389 |
| BssNI | GRCGYC | 1 cut(s) | 353 |
| BssT1I | CCWWGG | 2 cut(s) | 233, 554 |
| Bst4CI | ACNGT | 2 cut(s) | 193, 449 |
| Bst6I | CTCTTC | 2 cut(s) | 512, 557 |
| BstACI | GRCGYC | 1 cut(s) | 353 |
| BstC8I | GCNNGC | 1 cut(s) | 417 |
| BstDEI | CTNAG | 3 cut(s) | 75, 454, 479 |
| BstDSI | CCRYGG | 1 cut(s) | 501 |
| BstHHI | GCGC | 3 cut(s) | 40, 49, 60 |
| BstKTI | GATC | 3 cut(s) | 151, 286, 392 |
| BstMAI | GTCTC | 2 cut(s) | 73, 182 |
| BstMBI | GATC | 3 cut(s) | 148, 283, 389 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 44, 46, 55, 167 |
| BstSLI | GKGCMC | 1 cut(s) | 447 |
| BstV1I | GCAGC | 1 cut(s) | 391 |
| BstV2I | GAAGAC | 1 cut(s) | 187 |
| BsuI | GTATCC | 1 cut(s) | 95 |
| BtgI | CCRYGG | 1 cut(s) | 501 |
| BtsI | GCAGTG | 1 cut(s) | 465 |
| BtsIMutI | CAGTG | 1 cut(s) | 465 |
| Cac8I | GCNNGC | 1 cut(s) | 417 |
| CfoI | GCGC | 3 cut(s) | 40, 49, 60 |
| Cfr13I | GGNCC | 1 cut(s) | 71 |
| CseI | GACGC | 1 cut(s) | 361 |
| CviJI | RGCY | 4 cut(s) | 6, 404, 500, 506 |
| CviKI_1 | RGCY | 4 cut(s) | 6, 404, 500, 506 |
| DdeI | CTNAG | 3 cut(s) | 75, 454, 479 |
| DpnI | GATC | 3 cut(s) | 150, 285, 391 |
| DpnII | GATC | 3 cut(s) | 148, 283, 389 |
| DraIII | CACNNNGTG | 2 cut(s) | 302, 482 |
| Eam1104I | CTCTTC | 2 cut(s) | 512, 557 |
| EarI | CTCTTC | 2 cut(s) | 512, 557 |
| Eco130I | CCWWGG | 2 cut(s) | 233, 554 |
| Eco32I | GATATC | 1 cut(s) | 373 |
| Eco47I | GGWCC | 1 cut(s) | 71 |
| EcoO109I | RGGNCCY | 1 cut(s) | 71 |
| EcoRV | GATATC | 1 cut(s) | 373 |
| EcoT14I | CCWWGG | 2 cut(s) | 233, 554 |
| EcoT22I | ATGCAT | 1 cut(s) | 28 |
| ErhI | CCWWGG | 2 cut(s) | 233, 554 |
| Esp3I | CGTCTC | 1 cut(s) | 73 |
| FaiI | YATR | 5 cut(s) | 24, 28, 96, 396, 398 |
| FaqI | GGGAC | 1 cut(s) | 416 |
| FauI | CCCGC | 1 cut(s) | 296 |
| Fnu4HI | GCNGC | 1 cut(s) | 405 |
| Fsp4HI | GCNGC | 1 cut(s) | 405 |
| FspBI | CTAG | 1 cut(s) | 162 |
| GlaI | GCGC | 3 cut(s) | 39, 48, 59 |
| GluI | GCNGC | 1 cut(s) | 405 |
| HgaI | GACGC | 1 cut(s) | 361 |
| HhaI | GCGC | 3 cut(s) | 40, 49, 60 |
| Hin1I | GRCGYC | 1 cut(s) | 353 |
| Hin6I | GCGC | 3 cut(s) | 38, 47, 58 |
| HinP1I | GCGC | 3 cut(s) | 38, 47, 58 |
| HinfI | GANTC | 1 cut(s) | 347 |
| HphI | GGTGA | 1 cut(s) | 542 |
| Hpy166II | GTNNAC | 1 cut(s) | 366 |
| Hpy188I | TCNGA | 3 cut(s) | 78, 336, 457 |
| Hpy8I | GTNNAC | 1 cut(s) | 366 |
| Hpy99I | CGWCG | 1 cut(s) | 355 |
| HpyCH4III | ACNGT | 2 cut(s) | 193, 449 |
| HpyCH4IV | ACGT | 1 cut(s) | 339 |
| HpyCH4V | TGCA | 4 cut(s) | 26, 407, 419, 470 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 44, 46, 55, 167 |
| HpyF3I | CTNAG | 3 cut(s) | 75, 454, 479 |
| HpySE526I | ACGT | 1 cut(s) | 339 |
| Hsp92I | GRCGYC | 1 cut(s) | 353 |
| HspAI | GCGC | 3 cut(s) | 38, 47, 58 |
| Kzo9I | GATC | 3 cut(s) | 148, 283, 389 |
| LmnI | GCTCC | 1 cut(s) | 511 |
| LpnPI | CCDG | 5 cut(s) | 20, 129, 230, 369, 414 |
| Lsp1109I | GCAGC | 1 cut(s) | 391 |
| LweI | GCATC | 1 cut(s) | 349 |
| MaeI | CTAG | 1 cut(s) | 162 |
| MaeII | ACGT | 1 cut(s) | 339 |
| MaeIII | GTNAC | 4 cut(s) | 176, 340, 382, 528 |
| MalI | GATC | 3 cut(s) | 150, 285, 391 |
| MboI | GATC | 3 cut(s) | 148, 283, 389 |
| MboII | GAAGA | 3 cut(s) | 187, 529, 571 |
| MhlI | GDGCHC | 1 cut(s) | 447 |
| MluCI | AATT | 3 cut(s) | 107, 117, 248 |
| MmeI | TCCRAC | 2 cut(s) | 79, 137 |
| MnlI | CCTC | 7 cut(s) | 84, 455, 463, 479, 513, 519, 558 |
| Mph1103I | ATGCAT | 1 cut(s) | 28 |
| MseI | TTAA | 1 cut(s) | 86 |
| MslI | CAYNNNNRTG | 3 cut(s) | 21, 65, 183 |
| MspA1I | CMGCKG | 1 cut(s) | 404 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 44, 46, 55, 167 |
| NdeII | GATC | 3 cut(s) | 148, 283, 389 |
| NlaIV | GGNNCC | 4 cut(s) | 72, 135, 451, 507 |
| NmuCI | GTSAC | 3 cut(s) | 176, 382, 528 |
| NsiI | ATGCAT | 1 cut(s) | 28 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 359 |
| PfeI | GAWTC | 1 cut(s) | 347 |
| PkrI | GCNGC | 1 cut(s) | 406 |
| PpuMI | RGGWCCY | 1 cut(s) | 71 |
| Psp5II | RGGWCCY | 1 cut(s) | 71 |
| PspN4I | GGNNCC | 4 cut(s) | 72, 135, 451, 507 |
| PspPI | GGNCC | 1 cut(s) | 71 |
| PspPPI | RGGWCCY | 1 cut(s) | 71 |
| PvuII | CAGCTG | 1 cut(s) | 404 |
| RseI | CAYNNNNRTG | 3 cut(s) | 21, 65, 183 |
| SaqAI | TTAA | 1 cut(s) | 86 |
| SatI | GCNGC | 1 cut(s) | 405 |
| Sau3AI | GATC | 3 cut(s) | 148, 283, 389 |
| Sau96I | GGNCC | 1 cut(s) | 71 |
| SduI | GDGCHC | 1 cut(s) | 447 |
| SetI | ASST | 6 cut(s) | 342, 406, 425, 490, 524, 530 |
| SfaNI | GCATC | 1 cut(s) | 349 |
| SinI | GGWCC | 1 cut(s) | 71 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 21, 65, 183 |
| Sse9I | AATT | 3 cut(s) | 107, 117, 248 |
| SsiI | CCGC | 4 cut(s) | 124, 159, 170, 289 |
| SspMI | CTAG | 1 cut(s) | 162 |
| StyI | CCWWGG | 2 cut(s) | 233, 554 |
| TaaI | ACNGT | 2 cut(s) | 193, 449 |
| TaiI | ACGT | 1 cut(s) | 342 |
| TaqI | TCGA | 2 cut(s) | 350, 539 |
| TasI | AATT | 3 cut(s) | 107, 117, 248 |
| TfiI | GAWTC | 1 cut(s) | 347 |
| Tru1I | TTAA | 1 cut(s) | 86 |
| Tru9I | TTAA | 1 cut(s) | 86 |
| TscAI | CASTG | 1 cut(s) | 472 |
| TseFI | GTSAC | 3 cut(s) | 176, 382, 528 |
| TseI | GCWGC | 1 cut(s) | 404 |
| Tsp45I | GTSAC | 3 cut(s) | 176, 382, 528 |
| TspRI | CASTG | 1 cut(s) | 472 |
| VpaK11BI | GGWCC | 1 cut(s) | 71 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XspI | CTAG | 1 cut(s) | 162 |
| Zsp2I | ATGCAT | 1 cut(s) | 28 |