aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 58, 93 |
| AclWI | GGATC | 1 cut(s) | 212 |
| AdeI | CACNNNGTG | 1 cut(s) | 335 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 64, 77, 140, 292, 293, 294 |
| AluBI | AGCT | 1 cut(s) | 257 |
| AluI | AGCT | 1 cut(s) | 257 |
| Alw26I | GTCTC | 3 cut(s) | 7, 116, 213 |
| AlwI | GGATC | 1 cut(s) | 212 |
| AoxI | GGCC | 2 cut(s) | 351, 380 |
| ApeKI | GCWGC | 2 cut(s) | 257, 305 |
| AseI | ATTAAT | 1 cut(s) | 20 |
| AspS9I | GGNCC | 1 cut(s) | 5 |
| AsuHPI | GGTGA | 1 cut(s) | 395 |
| AvaII | GGWCC | 1 cut(s) | 5 |
| BbsI | GAAGAC | 1 cut(s) | 121 |
| BbvI | GCAGC | 2 cut(s) | 244, 292 |
| BceAI | ACGGC | 2 cut(s) | 191, 338 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BcoDI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BfaI | CTAG | 1 cut(s) | 96 |
| BfmI | CTRYAG | 1 cut(s) | 306 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BisI | GCNGC | 2 cut(s) | 258, 306 |
| BlsI | GCNGC | 2 cut(s) | 259, 307 |
| Bme18I | GGWCC | 1 cut(s) | 5 |
| BmgT120I | GGNCC | 1 cut(s) | 5 |
| BmiI | GGNNCC | 3 cut(s) | 6, 69, 360 |
| BpiI | GAAGAC | 1 cut(s) | 121 |
| BsaBI | GATNNNNATC | 1 cut(s) | 40 |
| BsaJI | CCNNGG | 2 cut(s) | 354, 407 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 330, 360 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 64, 77, 140, 292, 293, 294 |
| Bse8I | GATNNNNATC | 1 cut(s) | 40 |
| BseDI | CCNNGG | 2 cut(s) | 354, 407 |
| BseJI | GATNNNNATC | 1 cut(s) | 40 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 64, 77, 140, 292, 293, 294 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseXI | GCAGC | 2 cut(s) | 244, 292 |
| BshFI | GGCC | 2 cut(s) | 353, 382 |
| BslFI | GGGAC | 2 cut(s) | 182, 269 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 64, 77, 140, 292, 293, 294 |
| BsmAI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BsmBI | CGTCTC | 1 cut(s) | 7 |
| BsmFI | GGGAC | 2 cut(s) | 182, 269 |
| BsnI | GGCC | 2 cut(s) | 353, 382 |
| Bsp143I | GATC | 3 cut(s) | 82, 217, 242 |
| BspACI | CCGC | 2 cut(s) | 58, 93 |
| BspANI | GGCC | 2 cut(s) | 353, 382 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspLI | GGNNCC | 3 cut(s) | 6, 69, 360 |
| BspMAI | CTGCAG | 1 cut(s) | 310 |
| BspPI | GGATC | 1 cut(s) | 212 |
| BssECI | CCNNGG | 2 cut(s) | 354, 407 |
| BssMI | GATC | 3 cut(s) | 82, 217, 242 |
| BssT1I | CCWWGG | 1 cut(s) | 407 |
| Bst4CI | ACNGT | 1 cut(s) | 127 |
| Bst6I | CTCTTC | 1 cut(s) | 365 |
| BstC8I | GCNNGC | 1 cut(s) | 270 |
| BstDEI | CTNAG | 2 cut(s) | 9, 332 |
| BstDSI | CCRYGG | 1 cut(s) | 354 |
| BstKTI | GATC | 3 cut(s) | 85, 220, 245 |
| BstMAI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BstMBI | GATC | 3 cut(s) | 82, 217, 242 |
| BstSFI | CTRYAG | 1 cut(s) | 306 |
| BstV1I | GCAGC | 2 cut(s) | 244, 292 |
| BstV2I | GAAGAC | 1 cut(s) | 121 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuRI | GGCC | 2 cut(s) | 353, 382 |
| BtgI | CCRYGG | 1 cut(s) | 354 |
| BtsI | GCAGTG | 1 cut(s) | 318 |
| BtsIMutI | CAGTG | 1 cut(s) | 318 |
| Cac8I | GCNNGC | 1 cut(s) | 270 |
| Cfr13I | GGNCC | 1 cut(s) | 5 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 372, 407 |
| CviAII | CATG | 1 cut(s) | 299 |
| CviJI | RGCY | 4 cut(s) | 257, 353, 359, 382 |
| CviKI_1 | RGCY | 4 cut(s) | 257, 353, 359, 382 |
| DdeI | CTNAG | 2 cut(s) | 9, 332 |
| DpnI | GATC | 3 cut(s) | 84, 219, 244 |
| DpnII | GATC | 3 cut(s) | 82, 217, 242 |
| DraIII | CACNNNGTG | 1 cut(s) | 335 |
| Eam1104I | CTCTTC | 1 cut(s) | 365 |
| EarI | CTCTTC | 1 cut(s) | 365 |
| Eco130I | CCWWGG | 1 cut(s) | 407 |
| Eco47I | GGWCC | 1 cut(s) | 5 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5 |
| EcoT14I | CCWWGG | 1 cut(s) | 407 |
| ErhI | CCWWGG | 1 cut(s) | 407 |
| Esp3I | CGTCTC | 1 cut(s) | 7 |
| FaeI | CATG | 1 cut(s) | 302 |
| FaiI | YATR | 4 cut(s) | 30, 249, 251, 300 |
| FaqI | GGGAC | 2 cut(s) | 182, 269 |
| FatI | CATG | 1 cut(s) | 298 |
| Fnu4HI | GCNGC | 2 cut(s) | 258, 306 |
| Fsp4HI | GCNGC | 2 cut(s) | 258, 306 |
| FspBI | CTAG | 1 cut(s) | 96 |
| GluI | GCNGC | 2 cut(s) | 258, 306 |
| HaeIII | GGCC | 2 cut(s) | 353, 382 |
| Hin1II | CATG | 1 cut(s) | 302 |
| HinfI | GANTC | 1 cut(s) | 41 |
| HphI | GGTGA | 1 cut(s) | 395 |
| Hpy188I | TCNGA | 1 cut(s) | 12 |
| Hpy99I | CGWCG | 1 cut(s) | 139 |
| HpyAV | CCTTC | 1 cut(s) | 409 |
| HpyCH4III | ACNGT | 1 cut(s) | 127 |
| HpyCH4V | TGCA | 4 cut(s) | 260, 272, 308, 323 |
| HpyF3I | CTNAG | 2 cut(s) | 9, 332 |
| Hsp92II | CATG | 1 cut(s) | 302 |
| Kzo9I | GATC | 3 cut(s) | 82, 217, 242 |
| LmnI | GCTCC | 1 cut(s) | 364 |
| LpnPI | CCDG | 4 cut(s) | 63, 164, 267, 282 |
| Lsp1109I | GCAGC | 2 cut(s) | 244, 292 |
| MaeI | CTAG | 1 cut(s) | 96 |
| MaeIII | GTNAC | 1 cut(s) | 110 |
| MalI | GATC | 3 cut(s) | 84, 219, 244 |
| MboI | GATC | 3 cut(s) | 82, 217, 242 |
| MboII | GAAGA | 3 cut(s) | 121, 382, 424 |
| MfeI | CAATTG | 1 cut(s) | 233 |
| MluCI | AATT | 4 cut(s) | 21, 51, 182, 233 |
| MmeI | TCCRAC | 1 cut(s) | 13 |
| MnlI | CCTC | 7 cut(s) | 18, 233, 308, 332, 343, 366, 372 |
| MseI | TTAA | 2 cut(s) | 20, 278 |
| MslI | CAYNNNNRTG | 1 cut(s) | 117 |
| MspA1I | CMGCKG | 1 cut(s) | 257 |
| MunI | CAATTG | 1 cut(s) | 233 |
| NdeII | GATC | 3 cut(s) | 82, 217, 242 |
| NlaIII | CATG | 1 cut(s) | 302 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 69, 360 |
| NmuCI | GTSAC | 1 cut(s) | 110 |
| PfeI | GAWTC | 1 cut(s) | 41 |
| PkrI | GCNGC | 2 cut(s) | 259, 307 |
| PpuMI | RGGWCCY | 1 cut(s) | 5 |
| PshBI | ATTAAT | 1 cut(s) | 20 |
| Psp5II | RGGWCCY | 1 cut(s) | 5 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 69, 360 |
| PspPI | GGNCC | 1 cut(s) | 5 |
| PspPPI | RGGWCCY | 1 cut(s) | 5 |
| PstI | CTGCAG | 1 cut(s) | 310 |
| PvuII | CAGCTG | 1 cut(s) | 257 |
| RseI | CAYNNNNRTG | 1 cut(s) | 117 |
| SaqAI | TTAA | 2 cut(s) | 20, 278 |
| SatI | GCNGC | 2 cut(s) | 258, 306 |
| Sau3AI | GATC | 3 cut(s) | 82, 217, 242 |
| Sau96I | GGNCC | 1 cut(s) | 5 |
| SetI | ASST | 3 cut(s) | 259, 278, 343 |
| SfcI | CTRYAG | 1 cut(s) | 306 |
| SinI | GGWCC | 1 cut(s) | 5 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 117 |
| Sse9I | AATT | 4 cut(s) | 21, 51, 182, 233 |
| SsiI | CCGC | 2 cut(s) | 58, 93 |
| SspMI | CTAG | 1 cut(s) | 96 |
| StyI | CCWWGG | 1 cut(s) | 407 |
| TaaI | ACNGT | 1 cut(s) | 127 |
| TasI | AATT | 4 cut(s) | 21, 51, 182, 233 |
| TfiI | GAWTC | 1 cut(s) | 41 |
| Tru1I | TTAA | 2 cut(s) | 20, 278 |
| Tru9I | TTAA | 2 cut(s) | 20, 278 |
| TscAI | CASTG | 1 cut(s) | 325 |
| TseFI | GTSAC | 1 cut(s) | 110 |
| TseI | GCWGC | 2 cut(s) | 257, 305 |
| Tsp45I | GTSAC | 1 cut(s) | 110 |
| TspRI | CASTG | 1 cut(s) | 325 |
| VpaK11BI | GGWCC | 1 cut(s) | 5 |
| VspI | ATTAAT | 1 cut(s) | 20 |
| XspI | CTAG | 1 cut(s) | 96 |