aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 17 |
| AccB1I | GGYRCC | 1 cut(s) | 437 |
| AciI | CCGC | 4 cut(s) | 113, 147, 158, 277 |
| AclWI | GGATC | 1 cut(s) | 266 |
| AcuI | CTGAAG | 1 cut(s) | 305 |
| AfaI | GTAC | 1 cut(s) | 28 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 131, 194, 427, 428 |
| Alw26I | GTCTC | 2 cut(s) | 62, 170 |
| AlwI | GGATC | 1 cut(s) | 266 |
| AoxI | GGCC | 1 cut(s) | 486 |
| AspLEI | GCGC | 2 cut(s) | 18, 49 |
| AspS9I | GGNCC | 1 cut(s) | 60 |
| AsuHPI | GGTGA | 1 cut(s) | 146 |
| AvaII | GGWCC | 1 cut(s) | 60 |
| BanI | GGYRCC | 1 cut(s) | 437 |
| BbsI | GAAGAC | 1 cut(s) | 175 |
| BccI | CCATC | 1 cut(s) | 196 |
| BciVI | GTATCC | 1 cut(s) | 84 |
| BcoDI | GTCTC | 2 cut(s) | 62, 170 |
| BfaI | CTAG | 1 cut(s) | 150 |
| BfuI | GTATCC | 1 cut(s) | 84 |
| Bme18I | GGWCC | 1 cut(s) | 60 |
| BmgT120I | GGNCC | 1 cut(s) | 60 |
| BmiI | GGNNCC | 5 cut(s) | 61, 123, 439, 460, 495 |
| BmsI | GCATC | 2 cut(s) | 330, 337 |
| BpiI | GAAGAC | 1 cut(s) | 175 |
| BsaBI | GATNNNNATC | 1 cut(s) | 95 |
| BsaJI | CCNNGG | 1 cut(s) | 489 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 465, 495 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 131, 194, 427, 428 |
| Bse8I | GATNNNNATC | 1 cut(s) | 95 |
| BseDI | CCNNGG | 1 cut(s) | 489 |
| BseJI | GATNNNNATC | 1 cut(s) | 95 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 131, 194, 427, 428 |
| BseMII | CTCAG | 1 cut(s) | 78 |
| BshFI | GGCC | 1 cut(s) | 488 |
| BshNI | GGYRCC | 1 cut(s) | 437 |
| BslFI | GGGAC | 2 cut(s) | 236, 404 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 131, 194, 427, 428 |
| BsmAI | GTCTC | 2 cut(s) | 62, 170 |
| BsmFI | GGGAC | 2 cut(s) | 236, 404 |
| BsnI | GGCC | 1 cut(s) | 488 |
| Bsp143I | GATC | 3 cut(s) | 136, 271, 377 |
| Bsp19I | CCATGG | 1 cut(s) | 489 |
| BspACI | CCGC | 4 cut(s) | 113, 147, 158, 277 |
| BspANI | GGCC | 1 cut(s) | 488 |
| BspCNI | CTCAG | 1 cut(s) | 77 |
| BspLI | GGNNCC | 5 cut(s) | 61, 123, 439, 460, 495 |
| BspPI | GGATC | 1 cut(s) | 266 |
| BspT107I | GGYRCC | 1 cut(s) | 437 |
| BssECI | CCNNGG | 1 cut(s) | 489 |
| BssMI | GATC | 3 cut(s) | 136, 271, 377 |
| BssT1I | CCWWGG | 1 cut(s) | 489 |
| Bst4CI | ACNGT | 1 cut(s) | 181 |
| Bst6I | CTCTTC | 1 cut(s) | 500 |
| BstC8I | GCNNGC | 1 cut(s) | 405 |
| BstDEI | CTNAG | 3 cut(s) | 64, 221, 442 |
| BstDSI | CCRYGG | 1 cut(s) | 489 |
| BstHHI | GCGC | 2 cut(s) | 18, 49 |
| BstKTI | GATC | 3 cut(s) | 139, 274, 380 |
| BstMAI | GTCTC | 2 cut(s) | 62, 170 |
| BstMBI | GATC | 3 cut(s) | 136, 271, 377 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 155 |
| BstV2I | GAAGAC | 1 cut(s) | 175 |
| BsuI | GTATCC | 1 cut(s) | 84 |
| BsuRI | GGCC | 1 cut(s) | 488 |
| BtgI | CCRYGG | 1 cut(s) | 489 |
| Cac8I | GCNNGC | 1 cut(s) | 405 |
| CfoI | GCGC | 2 cut(s) | 18, 49 |
| Cfr13I | GGNCC | 1 cut(s) | 60 |
| Csp6I | GTAC | 1 cut(s) | 27 |
| CviAII | CATG | 2 cut(s) | 434, 490 |
| CviJI | RGCY | 2 cut(s) | 488, 494 |
| CviKI_1 | RGCY | 2 cut(s) | 488, 494 |
| CviQI | GTAC | 1 cut(s) | 27 |
| DdeI | CTNAG | 3 cut(s) | 64, 221, 442 |
| DpnI | GATC | 3 cut(s) | 138, 273, 379 |
| DpnII | GATC | 3 cut(s) | 136, 271, 377 |
| Eam1104I | CTCTTC | 1 cut(s) | 500 |
| EarI | CTCTTC | 1 cut(s) | 500 |
| Eco130I | CCWWGG | 1 cut(s) | 489 |
| Eco32I | GATATC | 1 cut(s) | 361 |
| Eco47I | GGWCC | 1 cut(s) | 60 |
| Eco57I | CTGAAG | 1 cut(s) | 305 |
| EcoO109I | RGGNCCY | 1 cut(s) | 60 |
| EcoRV | GATATC | 1 cut(s) | 361 |
| EcoT14I | CCWWGG | 1 cut(s) | 489 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| ErhI | CCWWGG | 1 cut(s) | 489 |
| FaeI | CATG | 2 cut(s) | 437, 493 |
| FaiI | YATR | 7 cut(s) | 6, 23, 25, 291, 386, 435, 491 |
| FaqI | GGGAC | 2 cut(s) | 236, 404 |
| FatI | CATG | 2 cut(s) | 433, 489 |
| FauI | CCCGC | 1 cut(s) | 284 |
| FspBI | CTAG | 1 cut(s) | 150 |
| FspI | TGCGCA | 1 cut(s) | 17 |
| GlaI | GCGC | 2 cut(s) | 17, 48 |
| HaeIII | GGCC | 1 cut(s) | 488 |
| HhaI | GCGC | 2 cut(s) | 18, 49 |
| Hin1II | CATG | 2 cut(s) | 437, 493 |
| Hin6I | GCGC | 2 cut(s) | 16, 47 |
| HinP1I | GCGC | 2 cut(s) | 16, 47 |
| HinfI | GANTC | 1 cut(s) | 335 |
| HphI | GGTGA | 1 cut(s) | 146 |
| Hpy166II | GTNNAC | 1 cut(s) | 354 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 1 cut(s) | 323 |
| Hpy8I | GTNNAC | 1 cut(s) | 354 |
| Hpy99I | CGWCG | 1 cut(s) | 540 |
| HpyCH4III | ACNGT | 1 cut(s) | 181 |
| HpyCH4IV | ACGT | 2 cut(s) | 327, 538 |
| HpyCH4V | TGCA | 3 cut(s) | 4, 36, 407 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 155 |
| HpyF3I | CTNAG | 3 cut(s) | 64, 221, 442 |
| HpySE526I | ACGT | 2 cut(s) | 327, 538 |
| Hsp92II | CATG | 2 cut(s) | 437, 493 |
| HspAI | GCGC | 2 cut(s) | 16, 47 |
| Kzo9I | GATC | 3 cut(s) | 136, 271, 377 |
| LmnI | GCTCC | 1 cut(s) | 499 |
| LpnPI | CCDG | 5 cut(s) | 117, 218, 308, 357, 417 |
| LweI | GCATC | 2 cut(s) | 330, 337 |
| MaeI | CTAG | 1 cut(s) | 150 |
| MaeII | ACGT | 2 cut(s) | 327, 538 |
| MaeIII | GTNAC | 3 cut(s) | 164, 370, 570 |
| MalI | GATC | 3 cut(s) | 138, 273, 379 |
| MboI | GATC | 3 cut(s) | 136, 271, 377 |
| MboII | GAAGA | 3 cut(s) | 175, 311, 517 |
| MluCI | AATT | 3 cut(s) | 72, 106, 236 |
| MmeI | TCCRAC | 2 cut(s) | 126, 436 |
| MnlI | CCTC | 4 cut(s) | 73, 443, 467, 478 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MseI | TTAA | 4 cut(s) | 468, 530, 543, 580 |
| MslI | CAYNNNNRTG | 3 cut(s) | 54, 171, 530 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 155 |
| NcoI | CCATGG | 1 cut(s) | 489 |
| NdeII | GATC | 3 cut(s) | 136, 271, 377 |
| NlaIII | CATG | 2 cut(s) | 437, 493 |
| NlaIV | GGNNCC | 5 cut(s) | 61, 123, 439, 460, 495 |
| NmuCI | GTSAC | 1 cut(s) | 164 |
| NsbI | TGCGCA | 1 cut(s) | 17 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| PfeI | GAWTC | 1 cut(s) | 335 |
| PpuMI | RGGWCCY | 1 cut(s) | 60 |
| Psp5II | RGGWCCY | 1 cut(s) | 60 |
| PspN4I | GGNNCC | 5 cut(s) | 61, 123, 439, 460, 495 |
| PspPI | GGNCC | 1 cut(s) | 60 |
| PspPPI | RGGWCCY | 1 cut(s) | 60 |
| RsaI | GTAC | 1 cut(s) | 28 |
| RsaNI | GTAC | 1 cut(s) | 27 |
| RseI | CAYNNNNRTG | 3 cut(s) | 54, 171, 530 |
| SaqAI | TTAA | 4 cut(s) | 468, 530, 543, 580 |
| Sau3AI | GATC | 3 cut(s) | 136, 271, 377 |
| Sau96I | GGNCC | 1 cut(s) | 60 |
| SetI | ASST | 8 cut(s) | 32, 80, 136, 330, 413, 443, 478, 541 |
| SfaNI | GCATC | 2 cut(s) | 330, 337 |
| SinI | GGWCC | 1 cut(s) | 60 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 54, 171, 530 |
| Sse9I | AATT | 3 cut(s) | 72, 106, 236 |
| SsiI | CCGC | 4 cut(s) | 113, 147, 158, 277 |
| SspMI | CTAG | 1 cut(s) | 150 |
| StyI | CCWWGG | 1 cut(s) | 489 |
| TaaI | ACNGT | 1 cut(s) | 181 |
| TaiI | ACGT | 2 cut(s) | 330, 541 |
| TaqI | TCGA | 1 cut(s) | 338 |
| TasI | AATT | 3 cut(s) | 72, 106, 236 |
| TfiI | GAWTC | 1 cut(s) | 335 |
| Tru1I | TTAA | 4 cut(s) | 468, 530, 543, 580 |
| Tru9I | TTAA | 4 cut(s) | 468, 530, 543, 580 |
| TseFI | GTSAC | 1 cut(s) | 164 |
| Tsp45I | GTSAC | 1 cut(s) | 164 |
| TspDTI | ATGAA | 1 cut(s) | 306 |
| VpaK11BI | GGWCC | 1 cut(s) | 60 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 506 |
| XspI | CTAG | 1 cut(s) | 150 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |